| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.88570661 |
| The ability of the C terminus of EEA 1 to dimerize correlates with its ability to bind to Rab 5 and early endosomes , whereas its binding to PtdIns3P is independent of dimerization . 0.88570661^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.89357444 |
| These data provide evidence that EEA 1 interacts with both Rab5a and Rab5b , and that the GTPase activities of the two proteins are differentially regulated in vivo . . 0.89357444^^^ Although EEA 1 CT and EEA 1 NT interacted strongly with wild type Rab5b in the two hybrid system , we detected no interaction with wild type Rab5a , even though GTPase deficient mutants of both Rab5a and Rab5b interacted equally well with EEA 1 . 0.50033244^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.67653056 |
| EEA 1 binds to both phosphtidylinositol 3 phosphate ( PtdIns3P ) and to Rab 5 GTP in vitro , but the functional role of this dual interaction at the endosomal membrane is unclear . 0.67653056^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| This complex includes a novel protein , Rabenosyn 5 , which , like the previously characterized Rab 5 effector early endosome antigen 1 ( EEA 1 ) , contains an FYVE finger domain and is recruited in a phosphatidylinositol 3 kinase dependent fashion to early endosomes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Established Afipia containing phagosomes possess neither early endosomal marker proteins [ early endosome antigen 1 ( EEA 1 ) , Rab 5 , transferrin receptor , trytophane aspartate containing coat protein ( TACO ) ] nor late endosomal or lysosomal proteins [ cathepsin D , beta glucuronidase , vacuolar proton pumping ATPase , rab 7 , mannose 6 phosphate receptor , vesicle associated membrane protein 8 , lysosome associated membrane proteins LAMP 1 and LAMP 2 ] . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Determinants of Rab 5 interaction with the N terminus of early endosome antigen 1 . ^^^ The Rab 5 effector early endosome antigen 1 ( EEA 1 ) is a parallel coiled coil homodimer with an N terminal C ( 2 ) H ( 2 ) Zn ( 2+ ) finger and a C terminal FYVE domain . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Moreover , in the presence of PI3K inhibitors Rab 5 was found to be active , as deduced from measurements of early endosome antigen 1 binding and by photobleaching recovery determinations . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Ectopically expressed ALS 2 protein localizes with Rab 5 and early endosome antigen 1 ( EEA 1 ) onto early endosomal compartments and stimulates the enlargement of endosomes in cultured cortical neurons . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| An additional requirement for endosome fusion is the protein Early Endosome Antigen 1 ( EEA 1 ) , and its recruitment to the endosome depends on phosphatidylinositol 3 phosphate [ PI ( 3 ) P ] and the Rab 5 GTPase . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| We show that RCVs progress normally through the early stages of phagosome maturation acquiring PI3P , early endosome antigen 1 , and Rab 5 , and loosing all or much of them within minutes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Because endocytosis is involved in different cellular events and guanosine triphosphatase ( GTPase ) Rab proteins play important roles in various aspects of the membrane traffic , our aim was to characterize the expression of Rab proteins in T . cruzi infected cardiomyocytes , which displayed a downregulation of Rab 7 and Rab 11 , whereas the expression of Rab5a was maintained in the infected cultures even after longer periods of parasite internalization , but early endosome antigen 1 was partially downregulated . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| We identified a novel C terminal membrane anchoring domain in Solo / Trio8 that is required for enhanced green fluorescent protein Solo / Trio8 localization to early endosomes ( positive for both early endosome antigen 1 [ EEA 1 ] and Rab 5 ) in COS 7 cells and primary cultured neurons . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| EEA 1 is associated with early endosomes since it co localizes by immunofluorescence with the transferrin receptor and with Rab 5 but not with Rab 7 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Confocal immunofluorescence microscopy of transfected HEp 2 cells revealed that the C terminal part ( residues 1277 1411 ) of EEA 1 colocalizes extensively with a GTPase deficient mutant of the early endosomal GTPase Rab 5 , while deletion of the FYVE finger or mutations that interfere with zinc binding cause a cytosolic localization . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Treatment of the Rab 5 Q79L expressing cells with nocodazole alone led to a spatial redistribution and a significant decrease in the size of EEA 1 positive structures , whereas their number increased . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| EEA 1 links PI ( 3 ) K function to Rab 5 regulation of endosome fusion . ^^^ Here we identify the early endosomal autoantigen EEA 1 which binds the PI ( 3 ) K product phosphatidylinositol 3 phosphate , as a new Rab 5 effector that is required for endosome fusion . ^^^ The association of EEA 1 with the endosomal membrane requires Rab 5 GTP and PI ( 3 ) K activity , and excess Rab 5 GTP stabilizes the membrane association of EEA 1 even when PI ( 3 ) K is inhibited . ^^^ The identification of EEA 1 as a direct Rab 5 effector provides a molecular link between PI ( 3 ) K and Rab 5 , and its restricted distribution to early endosomes indicates that EEA 1 may confer directionality to Rab 5 dependent endocytic transport . . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| The Rab 5 effector EEA 1 is a core component of endosome docking . ^^^ Here we show that Rab 5 interacting soluble proteins can completely substitute for cytosol in an in vivo endosome fusion assay , and that the Rab 5 effector EEA 1 is the only factor necessary to confer minimal fusion activity . ^^^ Rab 5 and other associated proteins seem to act upstream of EEA 1 , implying that Rab 5 effectors comprise both regulatory molecules and mechanical components of the membrane transport machinery . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| For the endosomal coiled coil protein EEA 1 , targeting requires a carboxy terminal FYVE domain , which interacts with Rab 5 and phosphatidylinositol 3 phosphate ( PI ( 3 ) P ) , whereas the Golgi protein GM 130 interacts with Golgi membranes via the protein GRASP 65 [ 3 ] [ 6 ] [ 7 ] . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Oligomeric complexes link Rab 5 effectors with NSF and drive membrane fusion via interactions between EEA 1 and syntaxin 13 . ^^^ We now demonstrate that the Rab 5 effectors EEA 1 and Rabaptin 5 / Rabex 5 exist on the membrane in high molecular weight oligomers , which also contain NSF . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| The Rab 5 effector EEA 1 interacts directly with syntaxin 6 . ^^^ We recently identified the early endosomal autoantigen EEA 1 as an effector of Rab 5 in endocytic membrane fusion . ^^^ The binding site for syntaxin 6 overlaps with that of Rab 5 GTP at the C terminus of EEA 1 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| In an in vitro assay , the Rab 5 effector protein EEA 1 was preferentially recruited by Rab 5 positive endosomes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Several Rab 5 effectors are required in homotypic endosome fusion , including EEA 1 , which mediates endosome membrane docking , as well as Rabaptin 5 10 Rabex 5 complex and phosphatidylinositol 3 kinase hVPS 34 . ^^^ However , whereas Rab 5 and Rabaptin 5 are symmetrically distributed between CCVs and early endosomes , EEA 1 is recruited selectively onto the membrane of early endosomes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| EEA 1 is an early endosomal Rab 5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| EGF also enhanced translocation of the Rab 5 effector , early endosomal autoantigen 1 ( EEA 1 ) , from cytosol to membrane . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Purification of EEA 1 from bovine brain cytosol using Rab 5 affinity chromatography and activity assays . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| We report the requirement for 3 phosphoinositides and acquisition of Rab 5 effector early endosome autoantigen ( EEA 1 ) as essential molecular events necessary for phagosomal maturation . ^^^ Microinjection into macrophages of antibodies against EEA 1 and the PI 3K hVPS 34 reduced acquisition of late endocytic markers by latex bead phagosomes , demonstrating an essential role of these Rab 5 effectors in phagosomal biogenesis . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| While the membrane association of EEA 1 was abolished in the presence of a dominant negative mutant of the endosomal GTPase Rab 5 , the localisation of Hrs to early endosomes was Rab 5 independent . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Finally , we found that the interaction of Rab 5 with EEA 1 was markedly diminished after Rab 5 ADP ribosylation by ExoS . . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| This compound inhibits endosome fusion in vitro , possibly by inhibiting the production of phosphatidylinositol ( PtdIns ) 3 P , which co regulates EEA 1 with Rab 5 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Sequential roles for phosphatidylinositol 3 phosphate and Rab 5 in tethering and fusion of early endosomes via their interaction with EEA 1 . ^^^ The COOH terminus of EEA 1 contains a FYVE domain that interacts specifically with phosphatidylinositol 3 phosphate ( PtdIns 3 P ) and a Rab 5 GTPase binding region adjacent to the FYVE domain . ^^^ The dual interaction of EEA 1 with both PtdIns 3 P and Rab 5 has been hypothesized to provide the specificity required to target EEA 1 to early endosomes . ^^^ To test this hypothesis , we generated truncated ( amino acids 1277 1411 ) and full length EEA 1 constructs containing point mutations in the COOH terminus that impair Rab 5 but not PtdIns 3 P binding . ^^^ These results suggest that association with Rab 5 is not necessary for the initial binding and tethering functions of EEA 1 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| In contrast , rab 5 was as expected , shown to bind rabaptin 5 and rabex 5 as well as the endosomal effector protein EEA 1 but not GM 130 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Wortmannin prevented PI3P accumulation and the recruitment of EEA 1 but did not affect the association of Rab 5 with the SCV . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| We have previously proposed a model in which the Rab 5 GTPase recruits EEA 1 to the early endosome both directly , by binding to EEA 1 , and indirectly , through the recruitment of the p150 / hVps34 PI 3 kinase and the production of PI [ 3 ] P in the endosomal membrane . ^^^ We find that both endogenous hVps 34 and p 150 are targeted to enlarged endosomal structures in cells expressing constitutively activated Rab 5 , where they are significantly colocalized with EEA 1 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| It has been reported that phosphatidylinositol 3 kinases and FYVE domain proteins , such as EEA 1 , can be recruited onto early endosomes and act as Rab 5 effectors . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Lipid rafts associated molecules , such as GM 1 ganglioside , were selectively included into macropinosomes , but Rab 5 effector early endosome autoantigen ( EEA 1 ) and lysosomal glycoprotein LAMP 1 were excluded from macropinosomes containing B . abortus induced by swimming internalization . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| None of the known markers of early endosomes [ Early Endosomes Antigen 1 ( EEA 1 ) , Rab 5 and Rab 11 ] colocalized with the CD2AP / CMS positive vesicles . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| By contrast , other markers for early endocytic vesicles including clathrin , Rab 5 and EEA 1 were present in the preparation but did not colocalize with the ASOR vesicles . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| RIN 3 expressed in HeLa cells localized to cytoplasmic vesicles and the RIN 3 positive vesicles contained Rab 5 but not the early endosomal marker EEA 1 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| We have also found that early endosomal markers Rab 5 and EEA 1 ( membrane association of the latter depends on Rab 5 and hVPS 34 ) co localized with EGF receptor in the juxtranuclear region during late stages of endocytosis , both in control and upon wortmannin treatment . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| The small GTPase Rab 5 as well as the PI ( 3 ) P binding proteins EEA 1 , SARA and CISK were found to be abundant within PI ( 3 ) P containing endosomal microdomains . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| In the presence of the constitutively positive mutant Rab 5 ( Q79L ) palmitoylation resulted in polarized targeting of GAD 65 to giant Rab5a positive axonal endosomes , characterized by the absence of the Rab5a effector molecule EEA 1 and the transferrin receptor . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| These lectin stained vesicles colocalized with lysosomal marker proteins such as Limp 1 and Lamp 2 , but not with the early endosomal markers Rab 5 and EEA 1 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| The mycobacterial phagosome retain some proteins required for fusion with endocytic vesicles including Rab 5 but lack others such as early endosomal autoantigen 1 ( EEA 1 ) . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Using confocal microscopy , we demonstrated that CXCR 4 is colocalized with EEA 1 , Rab 5 , Rab 4 , and Rab 11 , which are localized in early and recycling endosomes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| After internalization , InlB and HGF colocalize with Rab 5 , EEA 1 and the transferrin receptor in classical early endosomes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| SFV were sequestered in the Rab 7 domains , and they were sorted away from the early endosomes when these domains detached as separate transport carriers devoid of Rab 5 , Rab 4 , EEA 1 , Arf 1 , and transferrin . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| VacA was subsequently pinocytosed by a clathrin independent mechanism into cell peripheral early endocytic compartments lacking caveolin 1 , the Rab 5 effector early endosomes antigen 1 ( EEA 1 ) and transferrin . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| RabGAP 5 overexpression triggers a loss of the Rab 5 effector EEA 1 from endosomes and blocks endocytic trafficking . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| We found that p38alpha can phosphorylate the Rab 5 effectors EEA 1 and Rabenosyn 5 on Thr 1392 and Ser 215 , respectively , and these phosphorylation events regulate the recruitment of EEA 1 and Rabenosyn 5 to membranes . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| After internalization , receptors are targeted to the early endosomes characterized by the molecular markers EEA 1 and rab 5 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| By 15 30 min after interleukin 6 ( IL 6 ) treatment , up to two thirds of cytoplasmic Tyr phosphorylated STAT 3 can be associated with the purified early endosome fraction ( Rab 5 , EEA 1 , transferrin receptor , and clathrin positive fraction ) . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that IL 4 ( a Th 2 cytokine ) and prostaglandin E 2 ( PGE 2 ) synergistically induce Rab 5 and several Rab effector proteins , including Rin 1 and EEA 1 , and promote the formation of an enlarged early endocytic ( EEE ) compartment . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| They subsequently acquire Rab 5 and EEA 1 before homotypic fusion with other GEECs , and heterotypic fusion with endosomes containing cargo from the clathrin dependent endocytic pathway . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Because PIKfyveWT did not substantially colocalize with EEA 1 or Rab 5 positive endosomes in COS cells , the dynamic PIKfyve catalyzed PtdIns 3 to PtdIns 3 , 5 P 2 switch was suggested to drive away PIKfyveWT from early endosomes toward later compartments . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| These endosomes are involved in transferrin uptake but lack the classical endosomal markers Rab 5 and EEA 1 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| The underlying mechanism entails phosphorylation of EGFR at a short segment ( amino acids 1002 1022 ) containing multiple serines and threonines , as well as phosphorylation of two Rab 5 effectors , EEA 1 and GDI . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| Moreover , early endosomal marker proteins ( EEA 1 and Rab 5 ) appeared to closely associate or partially co localize with SPIN 90 in endosomes and an internalized FITC dextran and Texas Red EGF were found on the endosomes in association with SPIN 90 . ^^^ |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15075 and P20339 |
Pubmed |
SVM Score :0.0 |
| NA |
|