| Interacting proteins: Q15052 and Q13153 |
Pubmed |
SVM Score :0.0 |
| While overexpression of an active mutant of alpha PIX ( DeltaCH ) facilitated B ( a ) P induced activation of Cdc42 / Rac1 , PAK 1 , and JNK 1 , overexpression of mutated alphaPIX ( L383R , L384S ) , which lacks guanine nucleotide exchange factor activity , SH 3 domain deleted alphaPIX ( Delta SH 3 ) , which lacks the ability to bind PAK , kinase negative PAK 1 ( K299R ) , and kinase negative SEK 1 ( K220A , K224L ) inhibited B ( a ) P triggered JNK 1 activation . ^^^ |
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| Interacting proteins: Q15052 and Q13153 |
Pubmed |
SVM Score :0.0 |
| Using an active , truncated form of alphaPix ( amino acids 155 545 ) , we observe stimulation of Pak 1 kinase activity when alphaPix 155 545 is co expressed with Cdc 42 and wild type Pak 1 in COS 1 cells . ^^^ This activation does not occur when we co express a Pak 1 mutant unable to bind alphaPix . ^^^ The activation of wild type Pak 1 by alphaPix 155 545 also requires that alphaPix 155 545 retain functional exchange factor activity . ^^^ However , the Pak 1 ( H 83 , 86L ) mutant that does not bind Rac or Cdc 42 is activated in the absence of GTPase by alphaPix 155 545 and by a mutant of alphaPix 155 545 that no longer has exchange factor activity . ^^^ Pak 1 activity stimulated in vitro using GTPgammaS loaded Cdc 42 was also enhanced by recombinant alphaPix 155 545 in a binding dependent manner . ^^^ |
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| Interacting proteins: Q15052 and Q13153 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15052 and Q13153 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15052 and Q13153 |
Pubmed |
SVM Score :0.0 |
| NA |
|