| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Protein hCdc 21 forms a stable trimeric complex with two novel human Mcm proteins , p85Mcm and p105Mcm . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| We detected in HeLa cell extracts six proteins , CDC 47 , CDC46 / MCM5 , Cdc 21 , P1 / MCM3 , Mis 5 , and BM28 / MCM2 , by their binding to a specific antibody and by partial sequencing . ^^^ The human homologs of the MCM 2 ( BM 28 ) , Mis 5 , Cdc 21 , and CDC 47 proteins were tightly bound to a histone Sepharose column and purified to near homogeneity , whereas the P1 / MCM3 and CDC46 / MCM5 proteins passed through . ^^^ Among the four core histones , the human BM28 / MCM2 , Mis 5 , Cdc 21 , and CDC 47 proteins had high affinity for histone H 3 . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| This gene family includes Saccharomyces cerevisiae MCM 2 , MCM 3 , MCM5 / CDC46 and CDC 47 , S . pombe nda 1 , nda 4 , cdc 21 and mis 5 , and genes encoding human BM 28 , P1MCM3 and P1 . 1MCM3 and mouse P1MCM3 , most of which are considered to be required for the initiation of DNA replication . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The members of the MCM protein family , including MCM 2 , MCM 3 , Cdc 21 , CDC 46 , Mis 5 and CDC 47 , are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Our group purified MCM protein complexes consisting of MCM 2 , 4 ( Cdc 21 ) , 6 ( Mis 5 ) , and 7 ( CDC 47 ) proteins from HeLa cells by using histone Sepharose column chromatography ( Ishimi , Y . , Ichinose , S . , Omori , A . , Sato K . , and Kimura , H . ( 1996 ) J . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : In the fission yeast Schizosaccharomyces pombe , Nda 1 , Nda 4 , Mis 5 and Cdc 21 proteins belong to the MCM ( minichromosome maintenance ) protein family which is thought to have six members . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| We compared the effects of disruptions of cdc 19 ( + ) ( mcm 2 ( + ) ) , cdc 21 ( + ) ( mcm 4 ( + ) ) , nda 4 ( + ) ( mcm 5 ( + ) ) and mis 5 ( + ) ( mcm 6 ( + ) ) ; in all cases , the null mutants underwent delayed S phase but were unable to proceed through the cell cycle . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Using coimmunoprecipitation , we find that Mis5p ( MCM 6 ) and Cdc21p ( MCM 4 ) are tightly associated with one another in a core complex with which Cdc19p loosely associates . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| In this study , we have expressed and isolated the mouse Mcm 2 , 4 , 6 , 7 proteins from insect cells and characterized various mutant Mcm 4 , 6 , 7 complexes in which the conserved ATPase motifs of the Mcm 4 and Mcm 6 proteins were mutated . ^^^ Biochemical analyses of these mutant Mcm 4 , 6 , 7 complexes indicated that the ATP binding activity of the Mcm 6 protein in the complex is critical for DNA helicase activity and that the Mcm 4 protein may play a role in the single stranded DNA binding activity of the complex . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Inhibition of licensing with 6 dimethylaminopurine results in chromatin containing specifically bound MCM 4 and MCM 6 . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Following exit from mitosis , the Mcm 4 subunit of the cytosolic interphase complex undergoes dephosphorylation , and the Mcm 2 , Mcm 3 , or Mcm 6 subunits are then actively phosphorylated by kinase ( s ) other than cyclin dependent kinases ( Cdks ) or Cdc 7 . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| MCM 4 and MCM 6 are also phosphorylated by huCdc 7 albeit to less extent . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Finally , using protein protein cross linking we have shown that MCM 2 interacts directly with MCM 5 and MCM 6 ; MCM 5 with MCM 3 and MCM 2 ; and MCM 6 with MCM 2 and MCM 4 . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Cdc 23 shows genetic interactions with four MCM subunits : cdc 23 M36 and cdc 23 1E2 alleles both show synthetic phenotypes with mcm 2 ( cdc 19 P1 ) and mcm 6 ( mis 5 268 ) , and cdc 23 M36 is synthetically lethal with mcm 4 ( cdc 21 K46 ) and with mcm 5 ( nda 4 108 ) . ^^^ Two hybrid analysis demonstrates interactions at the protein protein level between Cdc 23 and Mcm 4 , Mcm 5 and Mcm 6 . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Biol . 19 , 8003 8015 ) , our data indicate distinct roles of Mcm 4 , Mcm 6 , and Mcm 7 subunits in activation of the DNA helicase activity of the Mcm4 / 6 / 7 complex . . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| The MCM 8 protein from HeLa cells , a new member of the MCM family , co isolates through several steps with MCM 6 and MCM 7 , and MCM 8 co immunoprecipitates with MCM 4 , MCM 6 and MCM 7 , proteins reportedly forming a helicase complex involved in initiation of DNA replication . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Twenty three of these genes were studied at the protein level ; expression of five ( MCM 4 , P = . 002 ; MCM 3 , P = . 030 ; MCM 6 , P = . 004 ; KPNA 2 , P = . 021 ; and geminin , P = . 004 ) was statistically significantly associated with overall survival in the validation set . ^^^ In a multivariable Cox model adjusted for tumor thickness , ulceration , age , and sex , expression of MCM 4 ( hazard ratio [ HR ] of death = 4 . 04 , 95 % confidence interval [ CI ] = 1 . 39 to 11 . 76 ; P = . 010 ) and MCM 6 ( HR of death = 7 . 42 , 95 % CI = 1 . 99 to 27 . 64 ; P = . 003 ) proteins was still statistically significantly associated with overall survival . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| Introducing mutations in the N terminal six Ser and Thr residues of MCM 4 reduced the inhibition by CDK2 / cyclin A , while EBV PK inhibited the helicase activities of both wild type and mutant MCM 4 6 7 hexamers , probably since EBV PK can phosphorylate MCM 6 and another site ( s ) of MCM 4 in addition to the N terminal residues . ^^^ |
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| Interacting proteins: Q14566 and P33991 |
Pubmed |
SVM Score :0.0 |
| NA |
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