| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| The first step of the biosynthesis , the transfer of N acetylglucosamine from UDP N acetylglucosamine to phosphatidylinositol , is mediated by at least three genes in mammalian cells ( PIG A , PIG H and PIG C ) and in yeast ( GPI 1 , GPI 2 and GPI3 / SPT14 / CWH6 ) . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| In mammalian cells , this reaction is mediated by a complex of PIG A , PIG H , PIG C , and GPI 1 . ^^^ A complex of PIG A , PIG H , and PIG C decreased to a nearly undetectable level , whereas a complex of PIG A and PIG H was easily detected . ^^^ Therefore , GPI 1 stabilizes the enzyme by tying up PIG C with a complex of PIG A and PIG H . . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| The mammalian N acetylglucosaminyl transferase for the first step in glycosylphosphatidylinositol biosynthesis has been shown to consist of at least four components : PIG A , PIG C , PIG H and GPI 1 . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| GPI GnT is a uniquely complex glycosyltransferase , consisting of at least four proteins , PIG A , PIG H , PIG C and GPI 1 . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| This chemically simple step is genetically complex because three or four genes are required in both yeast ( GPI 1 , GPI 2 and GPI 3 ) and mammals ( GPI 1 , PIG A , PIG H and PIG C ) , respectively . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| The human enzyme has six known subunits , at least four of which , GPI 1 , PIG A , PIG C , and PIG H , have functional homologs in the budding yeast Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| In the current study , the chromosomal assignment of Piga , as well as of Pigf and Pigh , two additional genes involved in GPI anchor biosynthesis , has been established using a mouse interspecific backcross mapping technique . ^^^ In contrast to Piga , both human and mouse Pigf and Pigh genes map to autosomes . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| Based on these results we assign specific roles in the biosynthesis of MBC to the following Pig proteins : PigI , PigG , PigA , PigJ , PigH , PigM , PigF and PigN . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| PIG A and PIG H , which participate in glycosylphosphatidylinositol anchor biosynthesis , form a protein complex in the endoplasmic reticulum . ^^^ To clarify this complexity , we analyzed the products of two cloned human genes , PIG A and PIG H . ^^^ Here we demonstrate 1 ) that PIG A is an ER transmembrane protein with a large cytoplasmic domain that has homology to a bacterial GlcNAc transferase and a small lumenal domain ; 2 ) that PIG H is a cytoplasmically oriented , ER associated protein ; and 3 ) that they form a protein complex . ^^^ Taken together with the cytoplasmic orientation of GlcNAc PI , these results indicated that PIG A and PIG H are subunits of the GPI GlcNAc transferase that transfers GlcNAc to PI on the cytoplasmic side of the ER . . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG A , PIG H , PIG C and GPI 1 . ^^^ Mammalian PIG A and PIG C are homologous to yeast GPI 3 and GPI 2 , respectively ; however , mammalian PIG H is not homologous to yeast GPI 1 . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| The products of three mammalian genes , PIG A , PIG C and PIG H , have previously been shown to be involved in the putative enzymic complex . ^^^ |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37287 and Q14442 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| In the current study , the chromosomal assignment of Piga , as well as of Pigf and Pigh , two additional genes involved in GPI anchor biosynthesis , has been established using a mouse interspecific backcross mapping technique . ^^^ In contrast to Piga , both human and mouse Pigf and Pigh genes map to autosomes . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| The first step of the biosynthesis , the transfer of N acetylglucosamine from UDP N acetylglucosamine to phosphatidylinositol , is mediated by at least three genes in mammalian cells ( PIG A , PIG H and PIG C ) and in yeast ( GPI 1 , GPI 2 and GPI3 / SPT14 / CWH6 ) . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| The products of three mammalian genes , PIG A , PIG C and PIG H , have previously been shown to be involved in the putative enzymic complex . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| In mammalian cells , this reaction is mediated by a complex of PIG A , PIG H , PIG C , and GPI 1 . ^^^ A complex of PIG A , PIG H , and PIG C decreased to a nearly undetectable level , whereas a complex of PIG A and PIG H was easily detected . ^^^ Therefore , GPI 1 stabilizes the enzyme by tying up PIG C with a complex of PIG A and PIG H . . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| The mammalian N acetylglucosaminyl transferase for the first step in glycosylphosphatidylinositol biosynthesis has been shown to consist of at least four components : PIG A , PIG C , PIG H and GPI 1 . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| GPI GnT is a uniquely complex glycosyltransferase , consisting of at least four proteins , PIG A , PIG H , PIG C and GPI 1 . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| This chemically simple step is genetically complex because three or four genes are required in both yeast ( GPI 1 , GPI 2 and GPI 3 ) and mammals ( GPI 1 , PIG A , PIG H and PIG C ) , respectively . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| Based on these results we assign specific roles in the biosynthesis of MBC to the following Pig proteins : PigI , PigG , PigA , PigJ , PigH , PigM , PigF and PigN . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| PIG A and PIG H , which participate in glycosylphosphatidylinositol anchor biosynthesis , form a protein complex in the endoplasmic reticulum . ^^^ To clarify this complexity , we analyzed the products of two cloned human genes , PIG A and PIG H . ^^^ Here we demonstrate 1 ) that PIG A is an ER transmembrane protein with a large cytoplasmic domain that has homology to a bacterial GlcNAc transferase and a small lumenal domain ; 2 ) that PIG H is a cytoplasmically oriented , ER associated protein ; and 3 ) that they form a protein complex . ^^^ Taken together with the cytoplasmic orientation of GlcNAc PI , these results indicated that PIG A and PIG H are subunits of the GPI GlcNAc transferase that transfers GlcNAc to PI on the cytoplasmic side of the ER . . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| Mammalian PIG A and PIG C are homologous to yeast GPI 3 and GPI 2 , respectively ; however , mammalian PIG H is not homologous to yeast GPI 1 . ^^^ The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG A , PIG H , PIG C and GPI 1 . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| The human enzyme has six known subunits , at least four of which , GPI 1 , PIG A , PIG C , and PIG H , have functional homologs in the budding yeast Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14442 and P37287 |
Pubmed |
SVM Score :0.0 |
| NA |
|