| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.96166978 |
| In vitro evidence suggests that GKRP reversibly binds to GK and inhibits its activity ; however , less is known about the in vivo function of GKRP . 0.96166978^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.57249246 |
| Coexpression of GLUT 2 , GK and GKRP in areas implied in feeding behavior might play a role in glucose sensing , in which GLUT 2 has a permissive role and the interactions of GK with GKRP made possible a real sensor activity . 0.57249246^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.60539222 |
| We confirmed association of glucokinase immunoreactivity with rat liver mitochondria using Percoll gradient centrifugation and demonstrated its association with the 68 kDa regulatory protein ( GKRP ) but not with the binding protein phosphofructokinase 2 / fructose bisphosphatase 2 . 0.60539222^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Co localization of the ketohexokinase and glucokinase regulator genes to a 500 kb region of chromosome 2p23 . ^^^ The glucokinase regulator ( GCKR ) is a 65 kDa protein that inhibits glucokinase ( hexokinase 4 ) in liver and pancreatic islet . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Organization of the human glucokinase regulator gene GCKR . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| A novel glucokinase regulator in pancreatic beta cells : precursor of propionyl CoA carboxylase beta subunit interacts with glucokinase and augments its activity . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Fructose 1 phosphate antagonizes this inhibition by causing dissociation of the glucokinase regulatory protein complex . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| In the presence of hexose 6 phosphates , partially purified islet glucokinase was inhibited by the hepatic glucokinase regulatory protein in a D fructose 1 phosphate sensitive manner . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| We propose modifications to the simple model relating glucose concentration and GK flux , including glucose induced overexpression of the normal allele and a role of glucokinase regulatory protein . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Nine other candidate genes potentially implicated in insulin secretion or insulin action have been tested for linkage with MODY in these families , including glucokinase regulatory protein , hexokinase 2 , insulin receptor substrate 1 , fatty acid binding protein 2 , glucagon like peptide 1 receptor , apolipoprotein C 2 , glycogen synthase , adenosine deaminase ( a marker for the MODY gene on chromosome 20 ) , and phosphoenolpyruvate carboxykinase . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| That this result can not be ascribed to the levels of glucokinase regulatory protein in islets is shown by direct measurement of its activity and mRNA . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Human glucokinase regulatory protein ( GCKR ) : cDNA and genomic cloning , complete primary structure , and chromosomal localization . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Heterologous expression and characterization of rat liver glucokinase regulatory protein . ^^^ These studies establish that the protein encoded by the cDNA is identical to the glucokinase regulatory protein and also validate tools with which to carry out structure function studies on the interaction of the regulatory protein with glucokinase . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Glucokinase regulatory protein is a physiological inhibitor of GK in the liver . ^^^ Glucokinase regulatory protein may interact with glucokinase in the hepatocyte nucleus . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Gene expression of glucokinase regulatory protein in regenerating rat liver . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| This region contains several potential candidate genes for obesity , including glucokinase regulatory protein ( GCKR ) and pro opiomelanocortin ( POMC ) . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| The mechanism ( s ) of these responses remains unknown but recent studies suggest that the intracellular localization of glucokinase , controlled by glucokinase regulatory protein , may be important . ^^^ Glucokinase regulatory protein gelled F actin , and gelation was specifically inhibited by glucokinase and the regulatory protein effectors fructose 1 phosphate ( F1P ) and fructose 6 phosphate ( F6P ) . ^^^ These results suggest that glucokinase regulatory protein may play a role in metabolism sensitive glucokinase localization in vivo . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| The genes encoding glucokinase regulatory protein and ketohexokinase co localize to mouse chromosome 5 . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Northern blot analyses revealed that this binding protein was not identical to the well known liver glucokinase regulatory protein . ^^^ This hitherto unknown new protein factor may have the function of a glucokinase regulatory protein in the pancreatic beta cell , which may regulate glucokinase enzyme activity in a glucose dependent manner . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Glucokinase regulatory protein is essential for the proper subcellular localisation of liver glucokinase . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| We have studied the intracellular distribution in vivo of glucokinase ( GK ) and glucokinase regulatory protein ( GKRP ) in livers of fasted and refed rats , using specific antibodies against both proteins and laser confocal fluorescence microscopy . ^^^ Intracellular distribution of hepatic glucokinase and glucokinase regulatory protein during the fasted to refed transition in rats . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Inhibition by Stearoyl CoA and glucokinase regulatory protein and thermal stability were assayed for all mutants kinetically similar to wild type glucokinase . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Mice mutant for glucokinase regulatory protein exhibit decreased liver glucokinase : a sequestration mechanism in metabolic regulation . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Nuclear import of hepatic glucokinase depends upon glucokinase regulatory protein , whereas export is due to a nuclear export signal sequence in glucokinase . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Anomeric specificity of human liver and B cell glucokinase : modulation by the glucokinase regulatory protein . ^^^ In the presence of 0 . 2 mM d fructose 6 phosphate , the glucokinase regulatory protein caused a concentration related inhibition of d glucose phosphorylation , such an effect fading out at high concentrations of either d glucose or glucokinase relative to that of its regulatory protein . ^^^ The phosphorylation of alpha d glucose by liver glucokinase appeared more resistant than that of beta d glucose to the inhibitory action of d fructose 6 phosphate , as mediated by the glucokinase regulatory protein . ^^^ It is proposed that this information , especially the novel findings concerning the anomeric difference in both Hill number and sensitivity to the glucokinase regulatory protein , should be taken into account when considering the respective contributions of alpha and beta d glucose to the overall phosphorylation of equilibrated d glucose by glucokinase . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Evidence for glucose and sorbitol induced nuclear export of glucokinase regulatory protein in hepatocytes . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Characterization of glucokinase regulatory protein deficient mice . ^^^ The glucokinase regulatory protein ( GKRP ) inhibits glucokinase competitively with respect to glucose by forming a protein protein complex with this enzyme . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Hepatic glucokinase is inhibited by a 68 kDa glucokinase regulatory protein ( GKRP ) that is expressed in molar excess . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Glucokinase and glucokinase regulatory protein : mutual dependence for nuclear localization . ^^^ Conditional expression of the glucokinase regulatory protein in insulinoma cells , under control of the reverse tetracycline dependent transactivator , was used to investigate whether expression of this protein de novo would alter the intracellular distribution of glucokinase . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Also , using RT PCR analysis we found a glucokinase regulatory protein mRNA similar to that previously reported in liver . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Hepatocytes from fa / fa rats have higher glucokinase activity and expression of the glucokinase regulatory protein and higher rates of glycolysis and lipogenesis , but lower rates of glycogen synthesis than hepatocytes from Fa / . controls . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| The GKAP fused with green fluorescent protein was located in the cytosol , where glucokinase phosphorylates glucose , and not in the nucleus where the glucokinase is retained inactive by the glucokinase regulatory protein . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Glucose increased the apparent affinity for inhibitors that are glucose analogs but not for the glucokinase regulatory protein or palmitoyl CoA . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| The first consensus motif , LSAXXVAG , corresponded to the glucokinase regulatory protein of the liver . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Evidence that glucokinase regulatory protein is expressed and interacts with glucokinase in rat brain . ^^^ Our previous description of functional glucokinase isoforms in the rat brain has opened new questions concerning the presence of glucokinase regulatory protein in the brain and the functional role of its interactions with glucokinase . ^^^ In this study , we found glucokinase regulatory protein mRNA in rat brain , pancreatic islets and liver . ^^^ In situ hybridization studies revealed the presence of glucokinase regulatory protein mRNA , the highest number of positive cells being found in the paraventricular nucleus of the hypothalamus . ^^^ Glucokinase regulatory protein gene expression gave rise to a protein of 69 kDa mainly in nuclear and soluble cell fractions . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Mutations in the glucokinase regulatory protein gene in 2p23 in obese French caucasians . ^^^ AIMS / HYPOTHESIS : Glucokinase regulatory protein ( GKRP ) controls the activity of glucokinase in liver but possibly also in some areas of the central nervous system , suggesting that it could play a role in body mass control . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Differences in regulatory properties between human and rat glucokinase regulatory protein . ^^^ The inhibition of glucokinase by rat and Xenopus GKRPs ( glucokinase regulatory protein ) is well documented . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| GLUT 2 , glucokinase and glucokinase regulatory protein mRNAs and proteins were present in these areas as determined by biochemical approaches . ^^^ This enzyme is considered to be the true glucose sensor because it catalyses the rate limiting step of glucose catabolism its activity being regulated by interaction with glucokinase regulatory protein , that functions as a metabolic sensor . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| V62M GCK does not respond to RO 0281675 , nor does it respond to the hepatic glucokinase regulatory protein ( GKRP ) . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Additionally , introduction of the glucokinase regulatory protein and protein kinase C zeta have been shown to improve glucose tolerance in non insulin dependent diabetes mellitus animal models . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Finally , none of the mutations tested appears to affect the interaction of gluco kinase with the glucokinase regulatory protein in the yeast two hybrid system . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| The glucokinase regulatory protein ( GRP ) plays a pivotal role in the regulation of metabolic flux in liver by the glucose phosphorylating enzyme glucokinase . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| AIM : The glucokinase regulatory protein gene is a candidate gene for Type 2 diabetes . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Here we report the identification of a TNF responsive serine / threonine protein kinase termed GCK related ( GCKR ) that likely signals via mitogen activated protein kinase ( MAPK ) / extracellular signal regulated kinase ( ERK ) kinase kinase 1 ( MEKK 1 ) to activate the SAPK pathway . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 and TANK individually formed weak interactions with germinal center kinase ( GCK ) related kinase ( GCKR ) . ^^^ In addition , CD 40 activated endogenous GCKR in primary B cells , implicating GCK family proteins in CD 40 mediated B cell functions . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Other molecules involved in this pathway include the protein kinases , ASK 1 , germinal center kinase ( GCK ) , hematopoietic progenitor kinase 1 ( HPK 1 ) , and GCK related kinase ( GCKR ) . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Although the TRAF 2 TRAF domain binds ASK 1 , GCK , and the highly related kinase GCKR , the RING finger domain is needed for their activation . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Glucokinase is inhibited in the liver by a regulatory protein ( GKRP ) whose effects are increased by Fru 6 P and suppressed by Fru 1 P . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Hepatic glucokinase ( GK ) is acutely regulated by binding to its nuclear anchored regulatory protein ( GKRP ) . ^^^ Although GK release by GKRP is tightly coupled to the rate of glycogen synthesis , the nature of this association is obscure . ^^^ To gain insight into this coupling mechanism under physiological stimulating conditions in primary rat hepatocytes , we analyzed the subcellular distribution of GK and GKRP with immunofluorescence , and glycogen deposition with glycogen cytochemical fluorescence , using confocal microscopy and quantitative image analysis . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| The N / C of GK regulatory protein ( GKRP ) did not change in response to the intraduodenal glucose infusion or the rise in plasma glucose and / or insulin levels . ^^^ The results suggest that GK but not GKRP translocates rapidly in a manner that corresponds with changes in the hepatic glucose balance in response to glucose ingestion in vivo . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| GKA 1 increased the affinity of GK for the competitive inhibitor mannoheptulose but did not affect the affinity for the inhibitors palmitoyl CoA and the endogenous 68 kDa regulator ( GK regulatory protein [ GKRP ] ) , which bind to allosteric sites or to N acetylglucosamine , which binds to the catalytic site . ^^^ In hepatocytes , GKA 1 and GKA 2 stimulated glucose phosphorylation , glycolysis , and glycogen synthesis to a similar extent as sorbitol , a precursor of fructose 1 phosphate , which indirectly activates GK through promoting its dissociation from GKRP . ^^^ In conclusion , GK activators are potential antihyperglycemic agents for the treatment of type 2 diabetes through the stimulation of hepatic glucose metabolism by a mechanism independent of GKRP . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Hepatic glucokinase is regulated by a 68 kDa regulatory protein ( GKRP ) that is both an inhibitor and nuclear receptor for glucokinase . ^^^ Thus , it acts in a complementary mechanism to GKRP , which also regulates glucokinase protein expression and compartmentation . . ^^^ |
|
| Interacting proteins: Q14397 and P35557 |
Pubmed |
SVM Score :0.0 |
| Glucokinase ( GK ) and its inhibitory protein , GK regulatory protein ( GKRP ) , were colocalized in the cytoplasm of hepatocytes . ^^^ In this case , GK and GKRP were colocalized in the nuclei of hepatocytes . ^^^ GK , but not GKRP , was translocated from the nucleus to the cytoplasm . ^^^ Glucotoxicity may result in the blunted response of hepatic glucose flux to elevated plasma glucose and / or insulin associated with impaired regulation of GK by GKRP in ZDF rats . . ^^^ Glucokinase ( GK ) and its inhibitory protein , GK regulatory protein ( GKRP ) , were colocalized in the cytoplasm of hepatocytes . ^^^ |
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