| RGS 16 inhibits signalling through the G alpha 13 Rho axis . ^^^ In contrast , classical RGS proteins ( such as RGS 16 and RGS 4 ) exhibit RGS domain dependent GAP activity on G alpha 1 and G alpha q , but not G alpha 12 or G alpha 13 ( ref 4 ) . ^^^ Here , we show that RGS 16 inhibits G alpha 13 mediated , RhoA dependent reversal of stellation and SRE activation . ^^^ The RGS 16 amino terminus binds G alpha 13 directly , resulting in translocation of G alpha 13 to detergent resistant membranes ( DRMs ) and reduced p115Rho GEF binding . ^^^ RGS 4 does not bind G alpha 13 or attenuate G alpha 13 dependent responses , and neither RGS 16 nor RGS 4 affects G alpha 12 mediated signalling . ^^^ |