| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| To obtain a biochemical marker for Arabidopsis eIF 3 , we cloned the Arabidopsis ortholog of the eIF 3 subunit eIF3b ( PRT 1 ) . eIF3e coimmunoprecipitated with CSN 7 , and eIF3c coimmunoprecipitated with eIF3e , eIF3b , CSN 8 , and CSN 1 . eIF3e directly interacted with CSN 7 and eIF3c . ^^^ Whereas eIF 3 , as detected through eIF3b , localized solely to the cytoplasm , eIF3e , like CSN 7 , was also found in the nucleus . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Saccharomyces cerevisiae protein Pci8p and human protein eIF3e / Int 6 interact with the eIF 3 core complex by binding to cognate eIF3b subunits . ^^^ We show that both Pci8p and human eIF3e / Int 6 expressed in budding yeast interact with the yeast eIF 3 complex in vivo and in vitro by binding to a discrete segment of its eIF3b subunit Prt1p and that human eIF3e / Int 6 interacts with the human eIF3b segment homologous to the Pci8p binding site of yeast Prt1p . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The binding of eIF 2 GTP tRNA ( 1 ) ( Met ) ternary complex ( TC ) to 40S subunits is impaired in yeast prt 1 1 ( eIF3b ) mutant extracts , but evidence is lacking that TC recruitment is a critical function of eIF 3 in vivo . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| We addressed the hypothesis that eIF 3 noncore subunits have accessory functions that help to regulate translation initiation , by focusing on the Arabidopsis thaliana eIF3h subunit . ^^^ Together , these data implicate eIF 3 in mRNA associated translation initiation events , such as scanning , start codon recognition , or reinitiation and suggest that poor translation initiation of specific mRNAs contributes to the pleiotropic spectrum of phenotypic defects in the eif3h mutant . . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| By performing a two hybrid screen with Int 6 as bait , we have isolated subunits belonging to all three complexes , namely eIF 3 p110 , Rpt 4 , CSN 3 and CSN 6 . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The homology is distributed throughout the entire protein , except for the amino terminus , and is particularly high in the central portion of the protein , which contains a putative RNA recognition motif . hPrt 1 is recognized by an antibody raised against eIF 3 , and an affinity purified antibody to recombinant hPrt 1 recognizes a protein migrating at 116 kDa in a purified eIF 3 preparation . ^^^ Far Western analysis shows that hPrt 1 interacts directly with the p 170 subunit of eIF 3 . ^^^ Taken together , these experiments demonstrate that hPrt 1 is a component of eIF 3 . ^^^ Our data , combined with those of Hershey and co workers , suggest that mammalian eIF 3 is composed of at least 10 subunits : p 170 , p 116 ( hPrt 1 ) , p 110 , p 66 , p 48 , p 47 , p 44 , p 40 , p 36 , and p35 . . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Using the yeast two hybrid genetic screen , we found that a portion of p 150 interacts with hPrt 1 , another subunit of eIF 3 , and cytokeratin 7 , an intermediate filament protein . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The Saccharomyces cerevisiae PRT 1 gene product Prt1p is a component of translation initiation factor eIF 3 , and mutations in PRT 1 inhibit translation initiation . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Purified yeast translational initiation factor eIF 3 is an RNA binding protein complex that contains the PRT 1 protein . ^^^ Immunoblotting shows that the 90 kDa subunit corresponds to the product of the PRT 1 gene whose mutant form , prt 1 1 , exhibits destabilization of methionyl tRNAi binding to 40 S ribosomal subunits . eIF 3 , and specifically the 62 kDa subunit , bind to RNA . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The question of whether the Prt 1 containing protein complex represents the yeast homologue of mammalian translation initiation factor eIF 3 is discussed . . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| If protein synthesis is inhibited at an early initiation step by the use of a prt 1 mutation ( affecting the initiation factor eIF 3 ) , the Ip and Fp mRNAs are very rapidly degraded even in YPG . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Anti SUI 1 antisera immunoprecipitate all of the subunits of eIF 3 , whereas antisera against the eIF 3 complex and the individual PRT 1 and GCD 10 subunits of eIF 3 immunoprecipitate SUI 1 . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| By two hybrid screening we have identified two partners that directly associate with TIF 34 : PRT 1 , a previously characterized eIF 3 subunit , and a novel protein of 33 kDa ( eIF 3 p33 ) which is part of the eIF 3 complex and has an RNA binding domain . ^^^ Our results provide support for both physical and functional interactions between three subunits , TIF 34 , PRT 1 and p 33 , in the eIF 3 complex . . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The PRT 1 , TIF 34 , GCD 10 , and SUI 1 proteins of Saccharomyces cerevisiae were found previously to copurify with eukaryotic translation initiation factor 3 ( eIF 3 ) activity . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The purified complex could rescue Met tRNAiMet binding to 40S ribosomes in defective extracts from a prt 1 mutant or extracts from which Nip1p had been depleted , indicating that it possesses a known biochemical activity of eIF 3 . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Another allele of CDC 33 , cdc 33 1 , along with mutations in CEG 1 , encoding the nuclear guanylyltransferase , were also synthetic lethal with xrn1Delta , whereas mutations in PRT 1 , encoding a subunit of eIF 3 , were not . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Related eIF 3 subunits TIF 32 and HCR 1 interact with an RNA recognition motif in PRT 1 required for eIF 3 integrity and ribosome binding . eIF 3 binds to 40S ribosomal subunits and stimulates recruitment of Met tRNAiMet and mRNA to the pre initiation complex . ^^^ The RNA recognition motif ( RRM ) of eIF 3 subunit PRT 1 interacted simultaneously with HCR 1 and with an internal domain of eIF 3 subunit TIF 32 that has sequence and functional similarity to HCR 1 . ^^^ Removal of the PRT 1 RRM resulted in dissociation of TIF 32 , NIP 1 , HCR 1 and eIF 5 from eIF 3 in vivo , and destroyed 40S ribosome binding by the residual PRT 1 TIF34 TIF 35 subcomplex . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| These results provide in vivo evidence that TIF 32 bridges PRT 1 and NIP 1 , and that eIFs 1 and 5 bind to NIP 1 , in native eIF 3 . ^^^ Heat treated prt 1 1 extracts are defective for Met tRNA ( 1 ) Met binding to 40S subunits , and we also observed defective 40S binding of mRNA , eIFs 1 and 5 and eIF 3 itself in these extracts . ^^^ We could rescue 40S binding of Met tRNA ( 1 ) Met and mRNA , and translation of luciferase mRNA , in a prt 1 1 extract almost as well with purified PN 2 subcomplex as with five subunit eIF 3 , whereas the P 45 subcomplex was nearly inactive . ^^^ Thus , several key functions of eIF 3 can be carried out by the PRT 1 TIF32 NIP 1 subcomplex . . ^^^ |
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| Interacting proteins: P55884 and Q14152 |
Pubmed |
SVM Score :0.0 |
| We found that mutating the RNP 1 motif in the predicted RRM domain in yeast eukaryotic initiation factor 3 ( eIF 3 ) subunit b / PRT1 ( prt 1 rnp1 ) impairs its direct interactions in vitro with both eIF3a / TIF32 and eIF3j / HCR1 . ^^^ The rnp 1 mutation in PRT 1 confers temperature sensitive translation initiation in vivo and reduces 40S binding of eIF 3 to native preinitiation complexes . ^^^ Several findings indicate that the rnp 1 lesion decreases recruitment of eIF 3 to the 40S subunit by HCR 1 : ( 1 ) rnp 1 strongly impairs the association of HCR 1 with PRT 1 without substantially disrupting the eIF 3 complex ; ( 2 ) rnp 1 impairs the 40S binding of eIF 3 more so than the 40S binding of HCR 1 ; ( 3 ) overexpressing HCR 1 R215I decreases the Ts ( ) phenotype and increases 40S bound eIF 3 in rnp 1 cells ; ( 4 ) the rnp 1 Ts ( ) phenotype is exacerbated by tif 32 Delta6 , which eliminates a binding determinant for HCR 1 in TIF 32 ; and ( 5 ) hcr1Delta impairs 40S binding of eIF 3 in otherwise wild type cells . ^^^ Thus , the PRT 1 RNP1 motif coordinates the functions of HCR 1 and TIF 32 in 40S binding of eIF 3 and is needed for optimal preinitiation complex assembly and AUG recognition in vivo . . ^^^ |
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