| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.56907474 |
| Cloning and characterization of the p 42 subunit of mammalian translation initiation factor 3 ( eIF 3 ) : demonstration that eIF 3 interacts with eIF 5 in mammalian cells . 0.56907474^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.58686261 |
| The eIF 5 bipartite motif is also important for its interaction with the eIF 3 complex through the NIP 1 encoded subunit of eIF 3 . 0.58686261^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.62753234 |
| We show that yeast eIF 5 can bridge interaction in vitro between eIF 3 and eIF 2 by binding simultaneously to the amino terminus of eIF 3 subunit NIP 1 and the amino terminal half of eIF2beta , dependent on a conserved bipartite motif in the carboxyl terminus of eIF 5 . 0.62753234^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The reaction requires factors eIF 3 , eIF4C , and eIF 5 ; Met tRNA and the Met tRNA binding system ; either GTP or GMP PNP ; ATP ; and factors C 1 and eIF4A . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Histones ( H 1 , H2A B , H 3 , and H 4 ) and protein synthesis initiation factors other than eIF 2 ( eIF 3 , eIF 4A , eIF 4B , and eIF 5 ) were not substrates or were very poor substrates for the purified dsRNA dependent protein kinase . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Five initiation factors , eIF 2 , eIF 3 , eIF 4A , eIF 4B , and eIF 5 , were purified from human HeLa cells . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Several initiation factors ( eIF 2 , eIF 3 , eIF 4A , eIF 4B , eIF 4C , eIF 4E and eIF 5 ) and elongation factor 1 were found to have no such discriminatory effect . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Met tRNAi . 40S [ the complex between the 40S ribosomal subunit and the ternary complex containing equimolar amounts of eukaryotic initiation factor 2 ( eIF 2 ) , GTP , and eukaryotic initiator methionyl tRNA ( Met tRNAi ) ] with a 60S ribosomal subunit in the presence of mRNA , cap binding protein ( with `` capped ' ' messengers ) , ATP , and the initiation factors eIF 3 , eIF 4a , 4b , 4c , and eIF 5 , results in the formation of an 80S initiation complex ( Met tRNAi . 80S . mRNA ) with concomitant hydrolysis of GTP and liberation of eIF 2 for recycling in subsequent initiation events . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Using an assay system which requires all the initiation factors for optimal activity it was possible to show that the 0 40 % ammonium sulfate cut of initiation factors ( containing eIF 3 and eIF 4B ) was sensitive to helenalin , while the 40 50 % ammonium sulfate cut ( containing eIF 2 and eIF 5 ) was not . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Identification of a translation initiation factor 3 ( eIF 3 ) core complex , conserved in yeast and mammals , that interacts with eIF 5 . ^^^ The fifth Prt1p associated protein was eIF 5 , an initiation factor not previously known to interact with eIF 3 . ^^^ These findings suggest that Tif32p , Nip1p , Prt1p , Tif34p , and Tif35p comprise an eIF 3 core complex , conserved between yeast and mammals , that stably interacts with eIF 5 . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| No interaction with recombinant eIF 5 or the initiation site RNA GCCACAAUGGCA was detected by NMR , but GST pull down experiments show that eIF 1 binds specifically to the p 110 subunit of eIF 3 . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Removal of the PRT 1 RRM resulted in dissociation of TIF 32 , NIP 1 , HCR 1 and eIF 5 from eIF 3 in vivo , and destroyed 40S ribosome binding by the residual PRT 1 TIF34 TIF 35 subcomplex . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Interestingly , eIF 5 CTD bound simultaneously to the eIF4G subunit of the cap binding complex and the NIP 1 subunit of eIF 3 . ^^^ We propose that eIF 5 CTD stimulates binding of Met tRNA ( 1 ) ( Met ) and mRNA to 40S subunits through interactions with eIF 2 , eIF 3 and eIF4G ; however , its most important function is to anchor eIF 5 to other components of the 48S complex in a manner required to couple GTP hydrolysis to AUG recognition during the scanning phase of initiation . . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| A subcomplex of three eIF 3 subunits binds eIF 1 and eIF 5 and stimulates ribosome binding of mRNA and tRNA ( 1 ) Met . ^^^ We found that a stable subcomplex of His 8 PRT1 , NIP 1 and TIF 32 ( PN 2 subcomplex ) could be affinity purified from a strain overexpressing these eIF 3 subunits . eIF 5 , eIF 1 and HCR 1 co purified with this subcomplex , but not with distinct His 8 PRT1 TIF 34 TIF35 ( P 45 ) or His 8 PRT1 TIF 32 ( P 2 ) sub complexes . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| We found that the vast majority of translation initiation factors ( eIF 2 , eIF2B , eIF 3 , eIF4A1 , eIF 5 and eIF5B ) , all three elongation factors ( eEF1A , eEF1B and eEF 2 ) and the termination factor eRF 1 are strictly excluded from nuclei . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Interactions of eukaryotic translation initiation factor 3 ( eIF 3 ) subunit NIP1 / c with eIF 1 and eIF 5 promote preinitiation complex assembly and regulate start codon selection . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Interestingly , we found that TC resides in a multifactor complex ( MFC ) with eIF 3 , eIF 1 , and the GTPase activating protein for eIF 2 , known as eIF 5 . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Consistent with this , both mutations diminished 40S bound TC , eIF 5 and eIF 3 in vivo , and deltaC impaired TC recruitment in vitro . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Recruitment of the eukaryotic translation initiation factor 2 ( eIF 2 ) GTP Met tRNAiMet ternary complex to the 40S ribosome is stimulated by multiple initiation factors in vitro , including eIF 3 , eIF 1 , eIF 5 , and eIF1A . ^^^ To define the factor requirements for these reactions in vivo , we examined the effects of depleting eIF 2 , eIF 3 , eIF 5 , or eIF4G in Saccharomyces cerevisiae cells on binding of the ternary complex , other initiation factors , and RPL41A mRNA to native 43S and 48S preinitiation complexes . ^^^ Depleting eIF 2 , eIF 3 , or eIF 5 reduced 40S binding of all constituents of the multifactor complex ( MFC ) , comprised of these three factors and eIF 1 , supporting a mechanism of coupled 40S binding by MFC components . 40S bound mRNA strongly accumulated in eIF 5 depleted cells , even though MFC binding to 40S subunits was reduced by eIF 5 depletion . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The yeast eIF 3 subunits TIF32 / a , NIP1 / c , and eIF 5 make critical connections with the 40S ribosome in vivo . ^^^ Initiation factor 3 ( eIF 3 ) forms a multifactor complex ( MFC ) with eIF 1 , eIF 2 , and eIF 5 that stimulates Met tRNA ( 1 ) ( Met ) binding to 40S ribosomes and promotes scanning or AUG recognition . ^^^ These results suggest that eIF 3 binds to the solvent side of the 40S subunit in a way that provides access to the interface side for the two eIF 3 segments ( NIP 1 NTD and TIF 32 CTD ) that interact with eIF 1 , eIF 5 , and the eIF2 / GTP / Met tRNA ( 1 ) ( Met ) ternary complex . . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| A multifactor complex of eIF 1 , eIF 2 , eIF 3 , eIF 5 , and tRNA ( 1 ) Met promotes initiation complex assembly and couples GTP hydrolysis to AUG recognition . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| We show that affinity tagged RLI 1 co purifies with eukaryotic translation initiation factor 3 ( eIF 3 ) , eIF 5 , and eIF 2 , but not with other translation initiation factors or with translation elongation or termination factors . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| In vivo , overexpression of eIF 2 and tRNA ( Met ) ( 1 ) suppresses the temperature sensitive phenotype of tif 5 7A altering eIF 5 CTD by increasing interaction of the mutant eIF 5 with eIF 2 by mass action and restoring its defective interaction with eIF 3 . ^^^ Our results suggest strongly that MFC formation is an ordered event involving specific enhancement of eIF 5 CTD binding to eIF 3 on its binding to eIF2beta . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Sucrose gradient sedimentation analysis of log versus stationary phase cells and eIF 3 mutant strain showed that the endogenous eIF5A co sedimented with the actively translating 80S ribosomes and polyribosomes in an RNase and EDTA sensitive manner . ^^^ |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P55010 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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