Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.6014004
The binding of mRNA to the ribosome is mediated by eukaryotic initiation factors eukaryotic initiation factor 4F ( eIF4F ) , eIF4B , eIF4A , and eIF 3 , eIF4F binds to the mRNA cap structure and , in combination with eIF4B , is believed to unwind the secondary structure in the 5 ' untranslated region to facilitate ribosome binding . eIF 3 associates with the 40S ribosomal subunit prior to mRNA binding . eIF4B copurifies with eIF 3 and eIF4F through several purification steps , suggesting the involvement of a multisubunit complex during translation initiation . 0.6014004^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Significant amounts of radioactivity were found associated with initiation factors eIF 2 , polypeptide 2 ( molecular weight 53 , 000 ) ; eIF 3 , polypeptides 2 and 4 ( molecular weights 110 , 000 and 67 , 000 ) ; and eIF 4B . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Both eIF 3 and eIF 4B preparations contained such an activity . ^^^ However , at least some of the activity was due to the presence of an as yet unidentified factor that contaminated the eIF 3 and eIF 4B preparations . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Using ribosomal washes as a source of eukaryotic protein synthesis initiation factors , we identified the well known cap binding proteins eIF 4B and 4E , but eIF 2 and eIF 3 as well . ^^^ Next to their well known roles in the initiation process , eIF 2 and eIF 3 also cross linked to the 5 ' cap . eIF 2 stimulated eIF 4B and 4E cross linking , an observation that has been previously described more extensively . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Stimulation of translation correlates with enhanced phosphorylation of eIF 4F , eIF 4B , eIF 2B , eIF 3 and ribosomal protein S 6 , whereas inhibition correlates with phosphorylation of eEF 2 and the alpha subunit of eIF 2 . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Differential stimulation of phosphorylation of initiation factors eIF 4F , eIF 4B , eIF 3 , and ribosomal protein S 6 by insulin and phorbol esters . ^^^ Exposure of quiescent , serum starved 3T3 L 1 cells to insulin promotes phosphorylation of initiation factors eIF 4F , eIF 4B , and eIF 3 p 120 , as well as ribosomal protein S 6 . ^^^ Phosphorylation of both the p 25 and p 220 subunits of eIF 4F is stimulated typically by 2 . 5 5 fold , with a 2 4 fold increase in phosphorylation of eIF 4B and eIF 3 p 120 . ^^^ Upon prolonged exposure to PMA , the cells are no longer responsive to this mitogen and no stimulation of phosphorylation of eIF 4F , eIF 4b , eIF 3 p 120 , or S 6 via a protein kinase C dependent mechanism is observed . ^^^ Addition of insulin to these down regulated cells leads to stimulation of phosphorylation of eIF 4F p 220 , ribosomal protein S 6 , and to a lesser extent , eIF 4B ; little or no stimulation of phosphorylation of eIF 4F p 25 and eIF 3 p 120 is observed . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The concentrations of eIF 3 , eIF 4A , and eIF 4B required for half maximal translation of the uncapped forms of beta globin mRNA and AMV RNA 4 are either the same or only slightly higher ( 1 . 5 to 2 fold ) than the concentrations required for the capped forms . ^^^ With STNV RNA the concentration of eIF 4F required for half maximal translation of either uncapped or capped STNV RNA is 3 nM , and the concentrations of eIF 3 , eIF 4A , and eIF 4B required for the two forms are also the same . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Further purification of the factor yields a preparation that contains one major polypeptide with a molecular weight of approximately 59 , 000 , This factor enhances the binding of globin mRNA to 40 S ribosomal subunits in the presence of eIF 2 , eIF 3 , eIF 4A , and either eIF 4B or eIF 4F and has been designated eIF 4G . . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The major proteins of polyribosomal informosomes do not coincide in terms of electrophoretic mobility with initiation factors eIF 2 , eIF 2A , eIF 3 , eIF 4A and eIF 4B . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Histones ( H 1 , H2A B , H 3 , and H 4 ) and protein synthesis initiation factors other than eIF 2 ( eIF 3 , eIF 4A , eIF 4B , and eIF 5 ) were not substrates or were very poor substrates for the purified dsRNA dependent protein kinase . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The defect in initiation factors has been variously attributed to inactivation of eucaryotic initiation factor 3 ( eIF 3 ) , eIF4B , or a cap binding protein ( CBP ) complex . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Five initiation factors , eIF 2 , eIF 3 , eIF 4A , eIF 4B , and eIF 5 , were purified from human HeLa cells . ^^^ Antisera raised in goats against rabbit eIF 3 and human eIF 2 , eIF 4A , and eIF 4B cross react with the cognate factors from both species . ^^^ On the basis of immunoblotting techniques , eIF 4A is highly conserved , eIF 2 alpha , eIF 3 , and eIF 4B are somewhat less conserved , and eIF 2 beta is the least conserved of the proteins examined . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The 0 to 40 % ammonium sulfate precipitates from both uninfected and infected cells contain eucaryotic initiation factor 3 ( eIF 3 ) , eIf 4B , and the cap binding protein ( CBP ) , which is detected by means of a cross linking assay , as well as other proteins . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Differential ammonium sulfate precipitation of the supernatant ribosome associated proteins from virus infected and mock infected cells indicated by reciprocal reconstitution studies that the defective ribosomal initiation factor ( s ) was ( were ) present primarily in the 0 40 % ammonium sulfate fraction that is considered to contain primarily eIF 3 and eIF 4B . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Several initiation factors ( eIF 2 , eIF 3 , eIF 4A , eIF 4B , eIF 4C , eIF 4E and eIF 5 ) and elongation factor 1 were found to have no such discriminatory effect . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The levels of initiation factors eIF 2 , eIF 3 , eIF 4A , and eIF 4B were quantitated by immunoblotting ; all are enriched in the cytoskeletal fraction relative to the soluble fraction . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Protease activated kinase 1 phosphorylated one subunit of eIF 3 ( Mr 130000 ) , eIF 4B and 40 S ribosomal protein S 10 . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Using an assay system which requires all the initiation factors for optimal activity it was possible to show that the 0 40 % ammonium sulfate cut of initiation factors ( containing eIF 3 and eIF 4B ) was sensitive to helenalin , while the 40 50 % ammonium sulfate cut ( containing eIF 2 and eIF 5 ) was not . ^^^ Only eIF 3 showed appreciable sensitivity to helenalin , while eIF 2 , eIF 3 and eIF 4B were all sensitive to inactivation by N ethylmaleimide . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Canonical translation factors exhibited slight ( eIF 2 and GEF ) or no ( eIF 4A , eIF 4B , eIF 4E , eIF 4F , eIF 3 , and eEF 1 alpha ) stimulatory activity on translation of TAR containing CAT mRNA . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
To further characterize La activity in translation and to identify other potential limiting factors , we assayed the ability of La protein as well as purified initiation factors , eIF 2 , guanine nucleotide exchange factor ( GEF ) , eIF 4A , eIF 4B , eIF 4F , and eIF 3 , to stimulate the synthesis of P 1 , the capsid precursor protein , in poliovirus type 1 ( Mahoney ) RNA programmed RRL . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Sepharose 6B gel filtration experiments with combinations of nonlabeled and 14C reductively methylated initiation factors ( eIF 4A , eIF 4B , eIF 4F , and eIF 3 ) provide evidence that both eIF 4B and eIF 4F , but not eIF 4A , interact with ribosomes in the presence of specific factors and ATP . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Eukaryotic initiation factor 2 ( eIF 2 ) , eIF 3 , and eIF4F were required for initiation ; eIF4B and to a lesser extent the pyrimidine tract binding protein stimulated this process . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Ribosomal binding and positioning on these mRNAs did not require the initiation factors eIF 3 , eIF4A , eIF4B , and eIF4F and translation of these mRNAs was not inhibited by a trans dominant eIF4A mutant . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The presence of other initiation factors such as eIF 3 , eIF 4A or eIF 4B mimics in part the stimulatory effect of magnesium ions and probably stabilizes the cleavage products of eIF 4G generated by 2Apro . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
We have now extended our analysis to other factors involved in the initiation of protein synthesis and we report here that eIF4B , the p 35 subunit of eIF 3 , and minor proportions of the alpha subunit of eIF 2 and the eIF4E binding protein 4E BP 1 are also cleaved to give rise to discrete fragments . ^^^ Purified recombinant caspase 3 is able to degrade eIF4B and eIF 3 ( p 35 ) in vitro , producing fragments of the same sizes as those seen in intact cells . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Hsp 27 specifically bound eIF4G during heat shock , preventing assembly of the cap initiation / eIF4F complex and trapping eIF4G in insoluble heat shock granules . eIF4G is a specific target of Hsp 27 , as eIF4E , eIF4A , Mnk 1 , poly ( A ) binding protein , eIF4B , and eIF 3 were not bound by Hsp 27 and were not recruited into insoluble complexes . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Inhibition occurs at the level of polypeptide chain initiation and is accompanied by the phosphorylation of the alpha subunit of initiation factor eIF 2 and the caspase dependent cleavage of initiation factors eIF4G , eIF4B , eIF2alpha and the p 35 subunit of eIF 3 . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
Reconstitution of initiation using fully fractionated translation components indicated that 48S complex formation on both IRESs requires eIF 2 , eIF 3 , eIF4A , eIF4B , eIF4F , and the pyrimidine tract binding protein ( PTB ) but that the FMDV IRES additionally requires ITAF ( 45 ) , also known as murine proliferation associated protein ( Mpp 1 ) , a proliferation dependent protein that is not expressed in murine brain cells . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
The cap binding complex eIF4F and the factors eIF4A and eIF4B are required for binding of 43S complexes ( comprising a 40S subunit , eIF2 / GTP / Met tRNAi and eIF 3 ) to the 5 ' end of capped mRNA but are not sufficient to promote ribosomal scanning to the initiation codon . eIF1A enhances the ability of eIF 1 to dissociate aberrantly assembled complexes from mRNA , and these factors synergistically mediate 48S complex assembly at the initiation codon . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
IRES interaction with translation initiation factors : functional characterization of novel RNA contacts with eIF 3 , eIF4B , and eIF4GII . ^^^ Mutation of this stem loop structure abrogated eIF4B , but not eIF 3 , binding to the IRES . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
These data suggest that TAV enters the host translation machinery at the eIF4B removal step to stabilize eIF 3 on the translating ribosome , thereby allowing translation of polycistronic viral RNA . . ^^^
Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P23588 and Q14152	Pubmed	SVM Score :0.0
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