Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.55055546
We show that yeast eIF 5 can bridge interaction in vitro between eIF 3 and eIF 2 by binding simultaneously to the amino terminus of eIF 3 subunit NIP 1 and the amino terminal half of eIF2beta , dependent on a conserved bipartite motif in the carboxyl terminus of eIF 5 . 0.55055546^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The integrity of eIF 2 and several eIF 3 polypeptides was also examined and likewise no alterations were detected . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The C0 . 5s of eIF 3 , eIF 2 and eIF 4F were found to be about the same . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The major proteins of polyribosomal informosomes do not coincide in terms of electrophoretic mobility with initiation factors eIF 2 , eIF 2A , eIF 3 , eIF 4A and eIF 4B . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
On the basis of immunoblotting techniques , eIF 4A is highly conserved , eIF 2 alpha , eIF 3 , and eIF 4B are somewhat less conserved , and eIF 2 beta is the least conserved of the proteins examined . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
This would explain why mutations in eIF 3 mimic eIF 2 alpha phosphorylation in allowing ribosomes to bypass the uORFs and reinitiate at GCN 4 . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Inhibition occurs at the level of polypeptide chain initiation and is accompanied by the phosphorylation of the alpha subunit of initiation factor eIF 2 and the caspase dependent cleavage of initiation factors eIF4G , eIF4B , eIF2alpha and the p 35 subunit of eIF 3 . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Therefore , at least in this way , the pioneer round of translation is distinct from steady state translation . eIF4GI , poly ( A ) binding protein ( PABP ) 1 , eIF 3 , eIF4AI , and eIF2alpha coimmunopurify with both CBP 80 and eIF4E , which suggests that each factor functions in both modes of translation . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The 133 , 000 10 g supernatant fraction prepared from ascites cells in 20 mM KCl ( low CKl supernatant ) contained the initiation factors EIF 1 and EIF 2 ( and the elongation factore EF 1 and EF 2 ) but lacked EIF 3 ; thus , low KCl supernatant could be used to assay for EIF 3 . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Significant amounts of radioactivity were found associated with initiation factors eIF 2 , polypeptide 2 ( molecular weight 53 , 000 ) ; eIF 3 , polypeptides 2 and 4 ( molecular weights 110 , 000 and 67 , 000 ) ; and eIF 4B . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Using ribosomal washes as a source of eukaryotic protein synthesis initiation factors , we identified the well known cap binding proteins eIF 4B and 4E , but eIF 2 and eIF 3 as well . ^^^ Next to their well known roles in the initiation process , eIF 2 and eIF 3 also cross linked to the 5 ' cap . eIF 2 stimulated eIF 4B and 4E cross linking , an observation that has been previously described more extensively . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Eukaryotic initiation factors eIF 2 and eIF 3 : interactions , structure and localization in ribosomal initiation complexes . ^^^ For binding of initiator tRNA and mRNA to the 40S ribosomal subunit , the initiation factors eIF 2 and eIF 3 are particularly important . ^^^ The location of eIF 2 and eIF 3 in these complexes as well as the interactions of the individual components have been analyzed by biochemical methods and electron microscopy . ^^^ The results obtained are summarized in this article , and a model is derived describing the spatial arrangement of eIF 2 and eIF 3 together with initiator tRNA and mRNA on the 40S subunit . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
When physiological substrates for casein kinase 2 are examined , glycogen synthase and eukaryotic initiation factor 3 ( eIF 3 ) ( p 120 ) are phosphorylated by the alpha subunit at a rate equivalent to that of the holoenzyme , while phosphorylation of eIF 3 ( p 67 ) is reduced 9 fold and eIF 2 beta is not modified . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Stimulation of translation correlates with enhanced phosphorylation of eIF 4F , eIF 4B , eIF 2B , eIF 3 and ribosomal protein S 6 , whereas inhibition correlates with phosphorylation of eEF 2 and the alpha subunit of eIF 2 . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The micromolar amounts of the factors and ribosomes are as follows : EF 1 alpha , 34 ; EF 2 , 5 . 2 ; eIF 2 , 1 . 5 ; eIF 3 , 0 . 7 ; eIF 4A , 3 . 0 , eIF 4F , 0 . 09 ; eIF ( iso ) 4F , 0 . 8 ; and 40 S ribosomal subunits , 3 . 2 . ^^^ The molar ratios of the factors to 40 S ribosomal subunits are approximately 11 : 1 for EF 1 alpha , 1 . 6 : 1 for EF 2 , 0 . 45 : 1 for eIF 2 , 0 . 2 : 1 for eIF 3 , 0 . 9 : 1 for eIF 4A , 0 . 03 : 1 for eIF 4F , and 0 . 25 : 1 for eIF ( iso ) 4F . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Only eIF 2 was capable of decreasing the frequency of initiation at the second AUG codon and promoting use of the first AUG at high mRNA concentrations ; eIF 3 , 4A , 4B , 4C + 4D , 4F and 5 were inactive . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
RNAs were transcribed from these plasmids ( pSTNV 1239 and pSTNV 54 ) and tested for their ability to bind to wheat germ 40 S ribosomal subunits in the presence of wheat germ initiation factors eIF 4A , eIF 4F , eIF 4G , eIF 3 , eIF 2 , Met tRNA , ATP , and guanosine 5 ' ( beta , gamma imino ) triphosphate ( GMP PNP ) . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Further purification of the factor yields a preparation that contains one major polypeptide with a molecular weight of approximately 59 , 000 , This factor enhances the binding of globin mRNA to 40 S ribosomal subunits in the presence of eIF 2 , eIF 3 , eIF 4A , and either eIF 4B or eIF 4F and has been designated eIF 4G . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Histones ( H 1 , H2A B , H 3 , and H 4 ) and protein synthesis initiation factors other than eIF 2 ( eIF 3 , eIF 4A , eIF 4B , and eIF 5 ) were not substrates or were very poor substrates for the purified dsRNA dependent protein kinase . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Several initiation factors ( eIF 2 , eIF 3 , eIF 4A , eIF 4B , eIF 4C , eIF 4E and eIF 5 ) and elongation factor 1 were found to have no such discriminatory effect . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Met tRNAi . 40S [ the complex between the 40S ribosomal subunit and the ternary complex containing equimolar amounts of eukaryotic initiation factor 2 ( eIF 2 ) , GTP , and eukaryotic initiator methionyl tRNA ( Met tRNAi ) ] with a 60S ribosomal subunit in the presence of mRNA , cap binding protein ( with `` capped ' ' messengers ) , ATP , and the initiation factors eIF 3 , eIF 4a , 4b , 4c , and eIF 5 , results in the formation of an 80S initiation complex ( Met tRNAi . 80S . mRNA ) with concomitant hydrolysis of GTP and liberation of eIF 2 for recycling in subsequent initiation events . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The levels of initiation factors eIF 2 , eIF 3 , eIF 4A , and eIF 4B were quantitated by immunoblotting ; all are enriched in the cytoskeletal fraction relative to the soluble fraction . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Spots identified as eukaryotic initiation factor ( eIF ) 2 alpha , eIF 2 beta , eIF 2 gamma , eIF 4A , and four eIF 3 proteins of less than 50 , 000 Da corresponded to moderately abundant lysate proteins . ^^^ The factor to ribosome ratio for eIF 2 was 0 . 8 , for the average eIF 3 protein about 0 . 6 , and for eIF 4A it was significantly higher at 3 . 0 . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Using an assay system which requires all the initiation factors for optimal activity it was possible to show that the 0 40 % ammonium sulfate cut of initiation factors ( containing eIF 3 and eIF 4B ) was sensitive to helenalin , while the 40 50 % ammonium sulfate cut ( containing eIF 2 and eIF 5 ) was not . ^^^ Only eIF 3 showed appreciable sensitivity to helenalin , while eIF 2 , eIF 3 and eIF 4B were all sensitive to inactivation by N ethylmaleimide . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Canonical translation factors exhibited slight ( eIF 2 and GEF ) or no ( eIF 4A , eIF 4B , eIF 4E , eIF 4F , eIF 3 , and eEF 1 alpha ) stimulatory activity on translation of TAR containing CAT mRNA . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
To further characterize La activity in translation and to identify other potential limiting factors , we assayed the ability of La protein as well as purified initiation factors , eIF 2 , guanine nucleotide exchange factor ( GEF ) , eIF 4A , eIF 4B , eIF 4F , and eIF 3 , to stimulate the synthesis of P 1 , the capsid precursor protein , in poliovirus type 1 ( Mahoney ) RNA programmed RRL . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The results , together with prior genetic experiments , indicate that SUI 1 is essential for eIF 3 activity and functions as part of eIF 3 and in concert with eIF 2 to promote eIF 2 GTP Met tRNAi ternary complex recognition of the initiator codon . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Eukaryotic initiation factor 2 ( eIF 2 ) , eIF 3 , and eIF4F were required for initiation ; eIF4B and to a lesser extent the pyrimidine tract binding protein stimulated this process . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Eukaryotic initiation factor 3 ( eIF 3 ) is a large multisubunit complex that stabilizes the ternary complex , eIF 2 10 GTP 10 tRNA ( Met ) 1 and promotes mRNA binding to the 40 S ribosomal subunit . eIF 3 also functions as a ribosome subunit anti association factor . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Detailed information about the binding sites for eIF 1A , eIF 2 , eIF 3 , and Met tRNA ( 1 ) on the 40S ribosomal subunit was derived from the AFM images . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
We have now extended our analysis to other factors involved in the initiation of protein synthesis and we report here that eIF4B , the p 35 subunit of eIF 3 , and minor proportions of the alpha subunit of eIF 2 and the eIF4E binding protein 4E BP 1 are also cleaved to give rise to discrete fragments . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Reconstitution of initiation using fully fractionated translation components indicated that 48S complex formation on both IRESs requires eIF 2 , eIF 3 , eIF4A , eIF4B , eIF4F , and the pyrimidine tract binding protein ( PTB ) but that the FMDV IRES additionally requires ITAF ( 45 ) , also known as murine proliferation associated protein ( Mpp 1 ) , a proliferation dependent protein that is not expressed in murine brain cells . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
We propose that eIF 5 CTD stimulates binding of Met tRNA ( 1 ) ( Met ) and mRNA to 40S subunits through interactions with eIF 2 , eIF 3 and eIF4G ; however , its most important function is to anchor eIF 5 to other components of the 48S complex in a manner required to couple GTP hydrolysis to AUG recognition during the scanning phase of initiation . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Initiation on the hepatitis C virus IRES is even simpler : 43S complexes containing only eIF 2 and eIF 3 bind directly to the initiation codon as a result of specific interaction of the IRES and the 40S subunit . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
With limited concentrations of eIF 2 , eIF 3 , and eIF4F , p 50 ( but not pyrimidine tract binding protein , which was taken for comparison ) strongly stimulates formation of the 48 S preinitiation complexes with beta globin mRNA . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Overexpressing the NIP 1 NTD sequestered eIF 1 eIF5 eIF 2 in a defective subcomplex that derepressed GCN 4 translation , providing the first in vivo evidence that association with eIF 3 promotes binding of eIF 2 and Met tRNA ( 1 ) ( Met ) to 40S ribosomes . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
We found that the vast majority of translation initiation factors ( eIF 2 , eIF2B , eIF 3 , eIF4A1 , eIF 5 and eIF5B ) , all three elongation factors ( eEF1A , eEF1B and eEF 2 ) and the termination factor eRF 1 are strictly excluded from nuclei . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Assembly of 48S ribosomal complexes on the IRES requires eukaryotic initiation factor 2 ( eIF 2 ) , eIF 3 , eIF4A , and the central domain of eIF4G to which eIF4A binds . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Domains of eIF1A that mediate binding to eIF 2 , eIF 3 and eIF5B and promote ternary complex recruitment in vivo . ^^^ The unstructured N terminus of eIF1A interacts with eIF 2 and eIF 3 and is required at low temperatures for a step following TC recruitment . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
We conclude that IFN blocks HCV replication through translational control programs involving PKR and P 56 to , respectively , target eIF 2 and eIF 3 dependent steps in the viral RNA translation initiation process . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Initiation factor 3 ( eIF 3 ) forms a multifactor complex ( MFC ) with eIF 1 , eIF 2 , and eIF 5 that stimulates Met tRNA ( 1 ) ( Met ) binding to 40S ribosomes and promotes scanning or AUG recognition . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
A multifactor complex of eIF 1 , eIF 2 , eIF 3 , eIF 5 , and tRNA ( 1 ) Met promotes initiation complex assembly and couples GTP hydrolysis to AUG recognition . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
We show that affinity tagged RLI 1 co purifies with eukaryotic translation initiation factor 3 ( eIF 3 ) , eIF 5 , and eIF 2 , but not with other translation initiation factors or with translation elongation or termination factors . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
In vivo , overexpression of eIF 2 and tRNA ( Met ) ( 1 ) suppresses the temperature sensitive phenotype of tif 5 7A altering eIF 5 CTD by increasing interaction of the mutant eIF 5 with eIF 2 by mass action and restoring its defective interaction with eIF 3 . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Using an RNA based affinity purification approach , we show here that HCV IRES RNA facilitates eIF 2 function through its interactions with eIF 3 and the 40S ribosomal subunit . ^^^ Intriguingly , however , one of these IRES mutants prevents stable association of both eIF 3 and eIF 2 , preventing initiator tRNA deposition and explaining the block in 80S assembly . ^^^ These data suggest that the IRES RNA coordinates interactions of eIF 3 and eIF 2 on the ribosome required to position the initiator tRNA on the mRNA in the ribosomal peptidyl tRNA site ( P site ) . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Interestingly , we found that TC resides in a multifactor complex ( MFC ) with eIF 3 , eIF 1 , and the GTPase activating protein for eIF 2 , known as eIF 5 . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
There is an absolute requirement for the initiation factors eIF 2 and eIF 3 and the scanning factor eIF 1 to form 48S complexes on the IRES . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The eukaryotic initiation factor ( eIF ) 5 HEAT domain mediates multifactor assembly and scanning with distinct interfaces to eIF 1 , eIF 2 , eIF 3 , and eIF4G . ^^^ This activity is mediated by the ability of its C terminal HEAT domain to interact with eIF 1 , eIF 2 , and eIF 3 in the multifactor complex and with eIF4G in the 48S complex . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
Eukaryotic translation initiation factor 3 ( eIF 3 ) and eIF 2 can promote mRNA binding to 40S subunits independently of eIF4G in yeast . ^^^ Recruitment of the eukaryotic translation initiation factor 2 ( eIF 2 ) GTP Met tRNAiMet ternary complex to the 40S ribosome is stimulated by multiple initiation factors in vitro , including eIF 3 , eIF 1 , eIF 5 , and eIF1A . ^^^ To define the factor requirements for these reactions in vivo , we examined the effects of depleting eIF 2 , eIF 3 , eIF 5 , or eIF4G in Saccharomyces cerevisiae cells on binding of the ternary complex , other initiation factors , and RPL41A mRNA to native 43S and 48S preinitiation complexes . ^^^ Depleting eIF 2 , eIF 3 , or eIF 5 reduced 40S binding of all constituents of the multifactor complex ( MFC ) , comprised of these three factors and eIF 1 , supporting a mechanism of coupled 40S binding by MFC components . 40S bound mRNA strongly accumulated in eIF 5 depleted cells , even though MFC binding to 40S subunits was reduced by eIF 5 depletion . ^^^ Depleting eIF 2 or eIF 3 impaired mRNA binding to free 40S subunits , but depleting eIF4G led unexpectedly to accumulation of mRNA on 40S subunits . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
The binding sites of eIF 2 beta , eIF 3 and eIF 1 were mapped onto the structure . eIF 2 beta and eIF 3 bind to non overlapping patches of negative and positive electrostatic potential , respectively . . ^^^
Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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Interacting proteins: P05198 and Q14152	Pubmed	SVM Score :0.0
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