| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.65645297 |
| Affinity purification of histidine tagged eIF3k from transiently transfected COS cells copurifies other eIF 3 subunits . eIF3k colocalizes with eIF 3 on 40S ribosomal subunits . eIF3k coexpressed with five other ' core ' eIF 3 subunits in baculovirus infected insect cells , forms a stable , immunoprecipitatable , complex with the ' core ' . eIF3k interacts directly with eIF3c , eIF3g and eIF3j by glutathione S transferase pull down assays . 0.65645297^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| As the largest subunit , eIF3a mediates most functions of eIF 3 . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Here we report that the 20S proteasome endoproteolytically cleaves the translation initiation factors eIF4G , a subunit of eIF4F , and eIF3a , a subunit of eIF 3 . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| L mimosine , a plant amino acid , can reversibly block mammalian cells at late G 1 phase and has been found to affect translation of mRNAs of the cyclin dependent kinase inhibitor p 27 , eIF3a ( eIF 3 p170 ) , and ribonucleotide reductase M 2 . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| A protein bound by PH 2 in Western blotting was identified as the 170 kDa subunit of eukaryotic translation initiation factor 3 ( eIF 3 p170 / eIF3a ) . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| In addition , we have used an in vitro 40 S ribosomal subunit binding assay to investigate subunit requirements for efficient association of the eIF 3 subcomplexes to the 40 S ribosomal subunit . eIF3j alone binds to the 40 S ribosomal subunit , and its presence is required for stable 40 S binding of an eIF3bgi subcomplex . ^^^ Furthermore , purified eIF 3 lacking eIF3j binds 40 S ribosomal subunits weakly , but binds tightly when eIF3j is added . ^^^ Cleavage of a 16 residue C terminal peptide from eIF3j by caspase 3 significantly reduces the affinity of eIF3j for the 40 S ribosomal subunit , and the cleaved form provides substantially less stabilization of purified eIF 3 40S complexes . ^^^ These results indicate that eIF3j , and especially its C terminus , play an important role in the recruitment of eIF 3 to the 40 S ribosomal subunit . . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| The multisubunit eukaryotic initiation factor ( eIF ) 3 plays various roles in translation initiation that all involve interaction with 40S ribosomal subunits . eIF 3 can be purified in two forms : with or without the loosely associated eIF3j subunit ( eIF3j+ and eIF3j , respectively ) . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Immunoblot analyses of sucrose gradient fractions for individual eIF 3 subunits show that the small eIF3j subunit is unassociated with the eIF 3 complex in quiescent T lymphocytes , but upon activation joins the other eIF 3 subunits and binds 40 S ribosomal subunits . ^^^ Because eIF3j has been shown to be required for eIF 3 binding to 40 S ribosomes in vitro , the results suggest that mitogenic stimulation of T lymphocytes leads to an activation of eIF3j , thereby enabling eIF 3 to bind to the larger ribosome free eIF 3 subunit complex , and then to the 40 S ribosomes . ^^^ The association of eIF3j with the other eIF 3 subunits appears to be inhibited by rapamycin , suggesting a mechanism that lies downstream from the mammalian target of rapamycin kinase . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| Here we report the cloning and characterization of human cDNAs encoding two more subunits of human eIF 3 , namely eIF 3 p44 and eIF 3 p35 . ^^^ |
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| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75822 and Q14152 |
Pubmed |
SVM Score :0.0 |
| NA |
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