| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Formation of the mammalian six layered neocortex depends on a signaling pathway that involves Reelin , the very low density lipoprotein receptor , the apolipoprotein E receptor 2 ( ApoER 2 ) , and the adaptor protein Disabled 1 ( Dab 1 ) . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Disabled 1 ( Dab 1 ) , an intracellular adapter protein containing a PTB ( phosphotyrosine binding ) domain , is tyrosyl phosphorylated during embryogenesis , but it accumulates in a hypophosphorylated form in mice lacking Reelin or both very low density lipoprotein receptor and apolipoprotein E receptor 2 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Mice deficient in Reelin , Disabled 1 ( Dab 1 ) , or both the very low density lipoprotein receptor ( VLDLR ) and the apolipoprotein E receptor 2 ( ApoER 2 ) exhibit identical neuroanatomic defects in laminar structures throughout the brain . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reelin is a large secreted protein that controls cortical layering by signaling through the very low density lipoprotein receptor and apolipoprotein E receptor 2 , thereby inducing tyrosine phosphorylation of the adaptor protein Disabled 1 ( Dab 1 ) and suppressing tau phosphorylation in vivo . ^^^ We present genetic evidence that this cascade is dependent on apolipoprotein E receptor 2 , very low density lipoprotein receptor , and Dab 1 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reelin , Disabled 1 ( Dab 1 ) , apolipoprotein E receptor 2 ( ApoER 2 ) , and very low density lipoprotein receptor ( VLDLR ) participate in a signaling pathway required for layer formation during mammalian brain development . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Mice that are mutant for Reelin or Dab 1 , or doubly mutant for the VLDL receptor ( VLDLR ) and ApoE receptor 2 ( ApoER 2 ) , show disorders of cerebral cortical lamination . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| In developing mammalian ( mouse ) brain , Reelin ( Reln ) is secreted by the Cajal Retzius ( CR ) neurons in the marginal zone , binds apolipoprotein E receptor 2 ( ApoER 2 ) and very low density lipoprotein receptor ( Vldlr ) , and induces the phosphorylation of the downstream cytoplasmic molecule disabled 1 ( Dab 1 ) in cortical plate neurons . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| The Reelin signaling pathway controls neuronal positioning during mammalian brain development by binding to the very low density lipoprotein receptor and apolipoprotein E receptor 2 , and signaling through the intracellular adapter protein Disabled 1 ( Dab 1 ) . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Because the Dab 1 phosphotyrosine binding domain can interact simultaneously with membrane lipids and with the intracellular domains of apolipoprotein E receptor 2 and very low density lipoprotein receptor , Dab 1 is preferentially recruited to the neuronal plasma membrane , where it is phosphorylated . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reelin binding to apolipoprotein E receptor 2 ( ApoER 2 ) and very low density lipoprotein receptor ( VLDLR ) leads to phosphorylation of disabled 1 ( Dab 1 ) , an adaptor protein which associates with the intracellular domains of both receptors . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reelin , a large protein that regulates neuronal migration during embryonic development , activates a conserved signaling pathway that requires its receptors , very low density lipoprotein receptor and apolipoprotein E receptor 2 , the cytoplasmic adaptor protein Disabled 1 ( Dab 1 ) , and Src family kinases ( SFK ) . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Genetic evidence suggests that interpretation of the Reelin signal by migrating neurons involves two neuronal cell surface proteins , the very low density lipoprotein receptor ( VLDLR ) and the apoE receptor 2 ( ApoER 2 ) as well as a cytosolic adaptor protein , Disabled 1 ( Dab 1 ) . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reelin binds to transmembrane receptors , including VLDLR and ApoER 2 , and stimulates tyrosine phosphorylation of Disabled 1 ( Dab 1 ) , which associates with an NPxY motif present in the cytoplasmic domain of the receptors . ^^^ Disruption of reelin , dab 1 , or both the vldr and apoer 2 genes results in similar cell positioning defects in laminated brain regions including the cerebellum , hippocampus , and cerebral cortex . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Biochemical and genetic arguments show that the extracellular matrix protein Reelin binds to two lipoprotein receptors ( VLDLR and ApoER 2 ) , which relay the Reelin signal inside target neurons by docking the tyrosine kinase adapter disabled 1 ( Dab 1 ) . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| ApoER 2 signaling appears to require interaction between its cytoplasmic domain and adapter molecules such as Dab 1 , JIP 1 and JIP 2 , and PSD 95 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Binding of Reelin to ApoER 2 and VLDLR induces phosphorylation of Dab 1 , which binds to the intracellular domains of both receptors . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Localization of ApoER 2 , VLDLR and Dab 1 in radial glia : groundwork for a new model of reelin action during cortical development . ^^^ Here we provide evidence that the reelin receptor machinery , including the lipoprotein receptors ApoER 2 and VLDLR along with the cytoplasmic adaptor protein Dab 1 , is located in radial glia precursors whose processes span the entire cortical wall from the ventricular zone to the pial surface . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| We studied the postnatal development of the radial glial scaffold in the dentate gyrus of reeler mice , lacking the extracellular matrix protein Reelin , in scrambler mice , deficient in the intracellular adaptor protein disabled 1 ( Dab 1 ) , which is required for the transmission of the Reelin signal into the cell , and in mutant mice lacking the Reelin receptors apolipoprotein receptor 2 ( ApoER 2 ) and / or the very low density lipoprotein receptor ( VLDLR ) , known to transmit the Reelin signal via Dab 1 . ^^^ By immunolabeling for the glial fibrillary acidic protein ( GFAP ) , we show that a regular dentate radial glial scaffold fails to form in mutants deficient of Reelin , Dab 1 , and VLDLR and ApoER 2 . ^^^ Our results suggest that Reelin signaling via ApoER 2 , VLDLR , and Dab 1 is required for the formation of a regular radial glial scaffold in the dentate gyrus . . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| The Reelin signal is transduced into the cell by the lipoprotein receptors VLDLR and ApoER 2 , leading to tyrosine phosphorylation of the associated intracellular adaptor protein Disabled 1 ( Dab 1 ) . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reln encodes an extracellular protein that regulates layer formation by interacting with VLDLR and ApoER 2 ( Lrp 8 ) receptors , thereby phosphorylating the Dab 1 signaling molecule . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Reelin is a secreted glycoprotein that regulates neuronal positioning in cortical brain structures through the VLDLR and ApoER 2 receptors and the adaptor protein Dab 1 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Finally , vldlr and apoER 2 are also expressed in migrating SPN , and mice lacking both vldlr and apoER 2 show aberrant SPN location that is identical to that of reeler and dab 1 null mice . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Binding of Reelin to the neuronal apolipoprotein E receptors apoER 2 and very low density lipoprotein receptor induces tyrosine phosphorylation of Dab 1 and the subsequent activation or relocalization of downstream targets like phosphatidylinositol 3 ( PI 3 ) kinase and Nckbeta . ^^^ The Reelin induced Dab 1 degradation also depends on apoER 2 and very low density lipoprotein receptor in a gene dose dependent manner . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Mice lacking Reelin ( Reln ) , double knockouts lacking the VLDL receptor ( VLDLR ) and ApoE receptor 2 ( ApoER 2 ) , and mice lacking disabled 1 ( Dab 1 ) display increased levels of phosphorylated tau . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We show that Reelin activates members of the Src family of non receptor tyrosine kinases ( SFKs ) and that this activation is dependent on the Reelin receptors apoER 2 and VLDLR and the adaptor protein Dab 1 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| The target cells for reelin express the cytoplasmic adapter protein Dab 1 , which binds to the reelin receptors VLDLR and ApoER 2 . ^^^ In mouse , some Cajal Retzius cells express reelin and VLDLR ; in humans , all the components of the signalling pathway ( Reelin , Dab 1 , VLDLR and ApoER 2 ) are present in subsets of Cajal Retzius cells . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Dab 1 interacts with NPXY motifs in the cytoplasmic tails of the lipoprotein receptors ApoER 2 and very low density lipoprotein receptor through an amino terminal phosphotyrosine binding ( PTB ) domain . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Within this pathway , Disabled 1 ( Dab 1 ) serves as an intracellular adaptor that is tyrosine phosphorylated when Reelin , a secreted glycoprotein , binds to the lipoprotein receptors VLDLR and ApoER 2 on the surface of neurons . ^^^ Here , we show that the phosphoinositide binding region within Dab 1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab 1 independently of VLDLR and ApoER 2 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Mutants lacking the reelin receptors ApoER 2 and Vldlr , and scrambler mice lacking Dab 1 , showed a normal complement of GnRH neurons in the hypothalamus , confirming that the effect of reelin in their migration is independent of Dab1 . . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| We have subsequently used these cell lines to study the subcellular localization of ApoER 2 and the VLDL receptor and could demonstrate that receptor mediated Dab 1 phosphorylation does not depend on lipid rafts and that phosphorylated Dab 1 remains bound to the receptor tail when the pathway is activated by Reelin . . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Conversely , reeler hippocampal slices were rescued by coculturing them with slices from Dab 1 ( / ) mutants or ApoER 2 ( / ) / VLDLR ( / ) mice . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Increased cell motility was also induced by knockdown of downstream components of the RELN pathway , including ApoER 2 , VLDLR , and DAB 1 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| Using primary neuronal cultures stimulated with recombinant Reelin , we find that normal Reelin signaling requires the wild type NFDNPVY sequence and likely the interaction of Apoer 2 with Dab 1 . ^^^ |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14114 and O75553 |
Pubmed |
SVM Score :0.0 |
| NA |
|