| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| NEDD 8 was activated by an E 1 ( Ub activating enzyme ) like complex , consisting of APP BP 1 and hUba 3 with high respective homologies to the amino and carboxy terminal regions of E 1 and then linked to hUbc 12 ( a human homolog of yeast Ub conjugating enzyme Ubc12p ) . ^^^ |
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| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| Expression , purification , and characterization of the E 1 for human NEDD 8 , the heterodimeric APPBP 1 UBA3 complex . ^^^ The heterodimeric activating enzyme of NEDD 8 , APPBP 1 UBA3 , plays an essential role in NEDD 8 conjugation . ^^^ The APPBP 1 UBA3 complex initiates NEDD 8 conjugation by first catalyzing adenylation of the C terminus of NEDD 8 and ultimately catalyzing transfer of NEDD 8 to the downstream enzyme in the pathway , Ubc 12 . ^^^ This chapter describes methods for expressing and purifying APPBP 1 UBA3 for in vitro studies of NEDD 8 conjugation . . ^^^ |
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| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| Moreover , human UBA 3 could form a beta mercaptoethanol sensitive conjugate with NEDD 8 in the presence of APP BP 1 , a protein with sequence homology to the N terminal half of ubiquitin activating enzyme . ^^^ |
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| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| Recent work suggests that APP BP 1 participates in a novel ubiquitinylation related pathway involving the ubiquitin like molecule NEDD 8 . ^^^ We show here that , in vivo in mammalian cells , APP BP 1 interacts with hUba 3 , its presumptive partner in the NEDD 8 activation pathway , and that the APP BP 1 binding site for hUba 3 is within amino acids 443 479 . ^^^ |
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| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| ASPP 2 inhibits APP BP 1 mediated NEDD 8 conjugation to cullin 1 and decreases APP BP 1 induced cell proliferation and neuronal apoptosis . ^^^ APP BP 1 , first identified as a protein that interacts with the carboxyl ( C ) terminus of the amyloid precursor protein ( APP ) , is one half of the bipartite activating enzyme for the ubiquitin like protein NEDD 8 . ^^^ ASPP 2 reduces the ability of NEDD 8 to conjugate to Cullin 1 , inhibits APP BP 1 dependent ts 41 cell proliferation , and blocks the ability of APP BP 1 to cause apoptosis and to cause DNA synthesis in neurons . ^^^ |
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| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| APP BP 1 , first identified as an amyloid precursor protein ( APP ) binding protein , is the regulatory subunit of the activating enzyme for the small ubiquitin like protein NEDD 8 . ^^^ A dominant negative mutant of the NEDD 8 conjugating enzyme hUbc 12 , which participates in the ubiquitin like pathway initiated by APP BP 1 , blocks neuronal apoptosis caused by APP , APP ( V642I ) , C 31 , or overexpression of APP BP 1 . ^^^ This translocation of APP BP 1 to lipid rafts is accompanied by a change in the subcellular localization of the ubiquitin like protein NEDD 8 , which is activated by APP BP1 . . ^^^ |
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| Interacting proteins: Q13564 and Q15843 |
Pubmed |
SVM Score :0.0 |
| The E 1 for Nedd 8 is a heterodimer of APP BP 1 and Uba 3 , while the E 2 is Ubc 12 . ^^^ |
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