| CaM KIIN interacted only with activated CaM KII ( 1 . e . , in the presence of Ca2+ / CaM or after autophosphorylation ) by using glutathione S transferase / CaM KIIN precipitations as well as coimmunoprecipitations from rat brain extracts or from HEK 293 cells cotransfected with both constructs . 1.339199^^^ Colocalization of CaM KIIN with activated CaM KII was demonstrated in COS 7 cells transfected with green fluorescent protein fused to CaM KIIN . 0.90427626^^^ |