| Interacting proteins: P61587 and Q13464 |
Pubmed |
SVM Score :0.89296296 |
| The interaction of RhoE with ROCK 1 was confirmed by coimmunoprecipitation of the endogenous proteins , and the two proteins colocalized on the trans Golgi network in COS 7 cells . 0.89296296^^^ Here , we show that RhoE binds ROCK 1 but none of the other RhoA targets tested . 0.85677694^^^ RhoE binds to ROCK 1 and inhibits downstream signaling . 0.63585837^^^ |
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| Interacting proteins: P61587 and Q13464 |
Pubmed |
SVM Score :0.0 |
| RhoE function is regulated by ROCK 1 mediated phosphorylation . ^^^ The Rho GTPase family member RhoE regulates actin filaments partly by binding to and inhibiting ROCK 1 , a serine / threonine kinase that induces actomyosin contractility . ^^^ Here , we show that ROCK 1 can phosphorylate multiple residues on RhoE in vitro . ^^^ In cells , ROCK 1 phosphorylated RhoE localizes in the cytosol , whereas unphosphorylated RhoE is primarily associated with membranes . ^^^ Phosphorylation has no effect on RhoE binding to ROCK 1 , but instead increases RhoE protein stability . ^^^ |
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| Interacting proteins: P61587 and Q13464 |
Pubmed |
SVM Score :0.0 |
| RhoE inhibits RhoA signalling in part by binding to the RhoA activated serine / threonine kinase ROCK 1 ( Rho associated kinase 1 ) , thereby preventing it from phosphorylating its targets . ^^^ RhoE activity is itself regulated by phosphorylation by ROCK 1 on multiple sites . ^^^ |
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| Interacting proteins: P61587 and Q13464 |
Pubmed |
SVM Score :0.0 |
| In part , this is due to RhoE interaction with the RhoA effector ROCK 1 , a serine / threonine kinase that regulates the formation and contractility of stress fibers . ^^^ RhoE binding inhibits ROCK 1 from phosphorylating its downstream target myosin light chain phosphatase , thus increasing the activity of the phosphatase to dephosphorylate myosin 2 , which results in reduced actomyosin contractility . ^^^ RhoE itself is phosphorylated by ROCK 1 , and this may enhance RhoE regulation of ROCK 1 function . ^^^ |
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