| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Inactivation of RhoA with C 3 transferase and inhibition of the Rho kinase p160ROCK with the pyridine derivative Y 27632 completely abolished activation of NHE 1 by integrins but not by platelet derived growth factor . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| The ubiquitously expressed Na H exchanger , NHE 1 , acts downstream of RhoA in a pathway regulating focal adhesion and actin stress fiber formation . p160ROCK , a serine / threonine protein kinase , is a direct RhoA target mediating RhoA induced assembly of focal adhesions and stress fibers . ^^^ In CCL 39 cells , NHE 1 activity was stimulated by expression of mutationally active p160ROCK , but not by mutationally active protein kinase N , another RhoA target kinase . ^^^ Expression of a dominant interfering p160ROCK inhibited RhoA , but not Cdc 42 or Rac activation of NEH 1 . ^^^ In addition , the p160ROCK specific inhibitor Y 27632 inhibited increases in NHE 1 activity in response to RhoA , and to lysophosphatidic acid ( LPA ) , which stimulates RhoA , and it also inhibited LPA increased phosphorylation of NHE 1 . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| It includes the MAP kinase cascade , the Ca / calmodulin system , several heterotrimeric G proteins ( Galpha 12 , Galpha 13 , Galphaq , and Galphai ) , small G proteins ( ras , cdc 42 , rhoA ) , and downstream kinases ( e . g . , p160ROCK ) . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND / AIMS : p160ROCK , a serine / threonine protein kinase , is a direct RhoA target mediating RhoA induced assembly of focal adhesions and stress fibers . ^^^ CONCLUSIONS : Our findings indicate that p160ROCK mediated actin stress fiber assembly is involved in the pathophysiology of hepatic fibrogenesis and suggest that inhibitors of the RhoA ROCK pathway might be useful therapeutically in liver fibrogenesis . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we found for the first time that a selective inhibitor of Rho associated kinase ( p160ROCK ) , Y 27632 , impeded the subcellular spreading of cathepsin D staining and promoted reclustering of cathepsin D toward the perinuclear region in the active RhoA transfected cells . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that p160ROCK , a serine / threonine kinase effector of RhoA , is both necessary and sufficient for RhoA mediated tail retraction . ^^^ Finally , we find that p160ROCK signaling negatively regulates integrin adhesions and that inhibition of RhoA results in an accumulation of beta 2 integrin in the unretracted tails . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| RhoA activation was followed by p160ROCK activation mediated by RhoA , which led to myosin light chain ( MLC ) phosphorylation , which was dependent on RhoA and p160ROCK activities . ^^^ The kinetics of MLC activation was similar to that of RhoA and p160ROCK . ^^^ Inhibition of either RhoA or p160ROCK did not block SDF 1alpha induced short term actin polymerization , but induced the formation of long spikes arising from the cell body , which were found to be microtubule based . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Inhibiting RhoA with either C 3 toxin or inhibiting p160Rock kinase , an effector of RhoA , with Y 27632 inhibited collapse , retraction , and the number of axons that showed lateral extensions . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Disruption of RhoA function through pharmacological inhibition of its effector kinase , p160ROCK , restores normal Rac 1 and Cdc 42 activity and rescues the motility defect in Lis1+ / neurons . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| TGF beta induced RhoA and p160ROCK activation is involved in the inhibition of Cdc25A with resultant cell cycle arrest . ^^^ In this article , we report that the absence of RhoA and p160ROCK activity in fibroblastic NIH 3T3 cells and its presence in epithelial NMuMG cells can at least partially explain the difference in the TGF beta growth response . ^^^ These data provide evidence that signaling through RhoA and p160ROCK is important in TGF beta inhibition of cell proliferation and links signaling components for epithelial transdifferentiation with regulation of cell cycle progression . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| The presence of Y 27632 , an inhibitor of Rho kinase ( p160ROCK ) , a key downstream effector of RhoA , significantly reduced focal adhesion and stress fiber formation induced by Thy 1 . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Inhibition of p160ROCK , a kinase effector of RhoA , only partially inhibited cell migration . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Binding to ROCK 1 , but not activation of SRF , correlated with the activity of RhoA in transformation . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| In addition to the RhoA effector ROCK 1 we identified cDNAs encoding Kinectin , mDia 2 ( a p 140 mDia related protein ) , and the guanine nucleotide exchange factor , mNET 1 . ^^^ ROCK 1 , Kinectin , and mDia 2 can bind the wild type forms of both RhoA and Cdc 42 in a GTP dependent manner in vitro . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequence of the protein deduced from the nucleotide sequence of the cDNA exhibited a close homology to that of a RhoA ( Ras related small GTPase ) associated serine threonine kinase ( ROCK 1 or Rho associated coiled coil kinase ) . ^^^ A 160 kDa RhoA binding polypeptide with a molecular mass similar to that of HEBM 1 and ROCK 1 was detected in the corneal epithelial extracts . ^^^ A close homology among the cDNA sequences of rabbit , mouse , rat , and human ROCK 1 indicates that this RhoA associated kinase is a well conserved protein . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| A series of RhoA / Rac1 and Rho / Ras chimeras was generated to map the domain ( s ) of RhoA involved in its association with two classes of effector kinase , represented by PRK 2 and ROCK 1 . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK 1 , an effector kinase involved in cytoskeletal reorganisation . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Both the rabbit and human SM expressed rhoA p 21 , ROCK 1 , and its isoform ROCK 2 . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| The aims of this study were to investigate the expression of RhoA , and the Rho kinases ROCK 1 and ROCK 2 in human pregnant myometrium , to evaluate the effects of Rho kinase inhibition on pregnant human myometrial contractility in vitro , and to compare these effects with those of the calcium channel blocker nifedipine . ^^^ RT PCR using primers for RhoA , ROCK 1 and ROCK 2 was performed on mRNA isolated from human pregnant myometrium . ^^^ Expression of RhoA , ROCK 1 and ROCK 2 mRNA was identified in human pregnant myometrium ( n = 3 ) . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Our results suggest that the BRG 1 protein affects the RhoA pathway by increasing the protein level of ROCK 1 , which allows stress fibre like structures to form . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Reduced [ Ca ( 2+ ) ] ( cyt ) , by either polyamine depletion or exposure to the Ca ( 2+ ) free medium , decreased RhoA protein expression , which was paralleled by significant decreases in GTP bound RhoA , ROCK 1 , and ROKalpha proteins , Rho kinase activity , and MLC phosphorylation . ^^^ Elevation of [ Ca ( 2+ ) ] ( cyt ) induced by the Ca ( 2+ ) ionophore ionomycin increased GTP bound RhoA , ROCK 1 , and ROKalpha proteins , Rho kinase activity , and MLC phosphorylation . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Here , we show that RhoE binds ROCK 1 but none of the other RhoA targets tested . ^^^ Although RhoE and RhoA were not able to bind ROCK 1 simultaneously , RhoE bound to the amino terminal region of ROCK 1 encompassing the kinase domain , at a site distant from the carboxy terminal RhoA binding site . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Among the functional proteins involved in the smooth muscle Ca2+ sensitization , CPI 17 expression was significantly reduced after the culture with IL 1beta , whereas the expressions of RhoA , ROCK 1 , ROCK 2 , MYPT 1 , myosin light chain kinase , and myosin phosphatase ( PP 1 ) were unchanged . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| PSCs expressed RhoA , ROCK 1 , and ROCK 2 . 5 . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Two central mediators of the signals from RhoA are the ROCK serine / threonine kinases ROCK 1 and ROCK 2 . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| RhoA , ROCK 1 , and ROCK 2 are expressed throughout chondrogenic differentiation . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| In revealing the mechanism underlying the translocation of eNOS , we found that a high level of LDL increased the level of membrane associated and GTP formed RhoA and activated the RhoA downstream kinase ROCK 1 activity . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Correlation of expression of RhoA ( RhoC and their effector ROCK 1 with malignant phenotype of ovarian cancer cells in vitro ] . ^^^ OBJECTIVE : To investigate the expression of RhoA , RhoC and their effector ROCK 1 in four ovarian cancer cell lines in vitro and their correlation with invasiveness . ^^^ METHODS : Expression of RhoA , RhoC and ROCK 1 mRNA and protein in four ovarian cancer cell lines SW 626 , Skov 3 , A 2780 and Caov 3 was detected by RT PCR and Western blot assay . ^^^ RESULTS : The expression levels of RhoA , RhoC and ROCK 1 mRNA and protein varied in the four different cell lines examined . ^^^ The expression level of RhoC , but not RhoA and ROCK 1 , was significantly correlated with the invasive capability of these cells in vitro ( r = 0 . 95 , P < 0 . 01 ) . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Elevated expression of RhoA and RhoC , as well as the Rho effector proteins ROCK 1 and ROCK 2 , are commonly observed in human cancers and are often associated with more invasive and metastatic phenotypes . ^^^ These results suggest that ROCK inhibitors would be useful antimetastatic and antiangiogenic chemotherapeutic agents in tumors associated with elevated RhoA , RhoC , ROCK 1 , or ROCK 2 expression . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Exposure of myocytes to LPA facilitated significant membrane translocation of RhoA and its downstream effector Rho kinase 1 ( ROCK 1 ) , and blocking this effect with Y 27632 appreciably reduced basal and HR LPL activity . ^^^ Comparable to LPA , hyperglycemia also caused significant membrane translocation of RhoA and ROCK 1 in hearts isolated from diazoxide treated animals , effects that were abrogated using insulin . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| In wild type fetuses , the myocardializing cells coexpress RhoA and one of its downstream mediators , ROCK 1 . ^^^ These data suggest that Vangl 2 is required for the polarization and movement of myocardializing cells into the outflow tract cushions , and that RhoA and ROCK 1 are downstream mediators of the PCP signaling pathway in the developing outflow tract . . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Pharmacological inhibition of intracellular contractility related myosin light chain kinase or RhoA kinase ( ROCK ) or expression of ROCK 1 small interfering RNA , dominant negative RhoA , or active Rac 1 decreased basal and TSA mediated histone H 3 acetylations in suspended cells , whereas inhibition of calmodulin dependent protein kinase 2 or transient overexpression of wild type myosin light chain kinase enhanced the acetylations . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| RhoE inhibits RhoA signalling in part by binding to the RhoA activated serine / threonine kinase ROCK 1 ( Rho associated kinase 1 ) , thereby preventing it from phosphorylating its targets . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Specific reduction of ROCK 1 by siRNA resulted in loss of stress fibers and focal adhesions , despite persistent ROCK 2 and guanine triphosphate bound RhoA . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| We found that either the small GTPase RhoA or its effector ROCK 1 ( Rho kinase ) , promotes membrane blebbing in astrocytes . ^^^ Ethanol induces a ROCK 1 activation that is mediated by RhoA , rather than by caspase 3 cleavage . ^^^ Accordingly , the RhoA inhibitor C 3 , completely abolishes the ethanol induced ROCK 1 activation . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| In part , this is due to RhoE interaction with the RhoA effector ROCK 1 , a serine / threonine kinase that regulates the formation and contractility of stress fibers . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| The RhoA and RhoC proteins regulate numerous effector proteins , with a central and vital signaling role mediated by the ROCK 1 and ROCK 2 serine / threonine kinases . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| Using the yeast two hybrid assay , we found that ICAP 1 binds the ROCK 1 kinase , an effector of the RhoA GTPase . ^^^ |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13464 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|