| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.5604609 |
| Here , we found that ILK stably forms a complex with ss affixin in platelets . 0.5604609^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.68131448 |
| Like affixin , gamma parvin directly associates with ILK through its CH 2 domain and colocalizes with ILK at focal adhesions as well as the leading edge of PMA stimulated U 937 cells plated on fibronectin . 0.68131448^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| Here , we identified a novel ILK binding protein , affixin , that consists of two tandem calponin homology domains . ^^^ In CHOcells , affixin and ILK colocalize at FAs and at the tip of the leading edge , whereas in skeletal muscle cells they colocalize at the sarcolemma where cells attach to the basal lamina , showing a striped pattern corresponding to cytoplasmic Z band striation . ^^^ When CHO cells are replated on fibronectin , affixin and ILK but not FA kinase and vinculin concentrate at the cell surface in blebs during the early stages of cell spreading , which will grow into membrane ruffles on lamellipodia . ^^^ Overexpression of the COOH terminal region of affixin , which is phosphorylated by ILK in vitro , blocks cell spreading at the initial stage , presumably by interfering with the formation of FAs and stress fibers . ^^^ These results provide evidence suggesting that affixin is involved in integrin ILK signaling required for the establishment of cell substrate adhesion . . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| In this study , we showed that the over expression of RP 1 corresponding to the first CH domain ( CH 1 ) of affixin , an integrin linked kinase ( ILK ) binding protein , induced a significant actin reorganization in MDCK cells by activating Cdc42 / Rac1 . ^^^ Our data strongly support that ILK and affixin provide a novel signalling pathway that links integrin signalling to Cdc42 / Rac1 activation . . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| Previously , we have reported that a novel integrin linked kinase ( ILK ) binding protein , affixin , is closely involved in the linkage between integrin and cytoskeleton in combination with ILK . ^^^ In the present work , we demonstrated that the second calponin homology domain of affixin directly interacts with alpha actinin in an ILK kinase activity dependent manner , suggesting that integrin ILK signaling evoked by substrate adhesion induces affixin alpha actinin interaction . ^^^ Time lapse observation revealed that in both experiments cells were round with small peripheral blebs and failed to develop lamellipodia , suggesting that the ILK affixin complex serves as an integrin anchoring site for alpha actinin and thereby mediates integrin signaling to alpha actinin , which has been shown to play a critical role in actin polymerization at focal adhesions . . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| The msq mutation ( L308P ) reduces ILK kinase activity and disrupts binding of ILK to the Z disc adaptor protein beta parvin ( Affixin ) . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| We analysed breast tumors and breast cancer cell lines for the expression of beta parvin ( ParvB ) , an adaptor protein that binds to the integrin linked kinase ( ILK ) . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| Both alpha parvin ( PARVA ) and beta parvin ( PARVB ) localize to focal adhesions and function in cell adhesion , spreading , motility and survival through interactions with partners , such as integrin linked kinase ( ILK ) , paxillin , alpha actinin and testicular kinase 1 . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| Whereas alpha parvin is not expressed in hematopoietic cells , beta parvin is only found in myeloid cells and gamma parvin is present in both cells of the myeloid and lymphoid lineages , where it binds ILK . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| PARVB is known to interact with integrin linked kinase ( ILK ) and is involved in the early stage of cell substrate interaction through integrins . ^^^ The identification of PARVB as an ARHGEF 6 interacting partner together with the co localization of ARHGEF 6 and ILK in spreading cells suggest that ARHGEF 6 is involved in integrin mediated signaling leading to activation of the GTPases Rac 1 and / or Cdc42 . . ^^^ |
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| Interacting proteins: Q9HBI1 and Q13418 |
Pubmed |
SVM Score :0.0 |
| We show here that CH ILKBP / actopaxin / alpha parvin and affixin / beta parvin ( abbreviated as alpha and beta parvin , respectively ) , two structurally closely related integrin linked kinase ( ILK ) binding focal adhesion proteins , are co expressed in human cells . ^^^ Loss of PINCH 1 or ILK , to which alpha and beta parvin bind , significantly reduced the activation of Rac , a key signaling event that controls lamellipodium formation and cell spreading . ^^^ Overexpression of beta parvin , however , was insufficient for stimulation of Rac activation or lamellipodium formation , although it was sufficient for promotion of apoptosis , another important cellular process that is regulated by PINCH 1 , ILK , and alpha parvin . ^^^ In addition , we show that the interactions of ILK with alpha and beta parvin are mutually exclusive . ^^^ Overexpression of beta parvin or its CH ( 2 ) fragment , but not a CH ( 2 ) deletion mutant , inhibited the ILK alpha parvin complex formation . ^^^ |
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