| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| ILK knock out also resulted in the suppression of phosphorylation of GSK 3beta on Ser 9 and cyclin D 1 expression . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Two cellular targets of ILK 1 , glycogen synthase kinase 3 beta ( GSK3beta ) and protein kinase B ( PKB ) / AKT , were differentially affected by ILKAP mediated inhibition of ILK 1 . ^^^ We propose that ILKAP regulates ILK 1 activity , targeting ILK 1 signalling of Wnt pathway components via modulation of GSK3beta phosphorylation . . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| ILK activation of the cyclin D 1 promoter was abolished by point mutation of a cAMP responsive element binding protein ( CREB ) / ATF 2 binding site at nucleotide 54 in the cyclin D 1 promoter , and by overexpression of either glycogen synthase kinase 3beta ( GSK 3beta ) or dominant negative mutants of CREB or ATF 2 . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Transient transfection of wild type GSK 3beta in ILK overexpressing cells decreased MMP 9 promoter activity and AP 1 activity , indicating that ILK can stimulate MMP 9 expression via GSK 3beta and AP 1 transcription factor . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| These include inhibition of , both the biochemical activation of ILK , inhibition of serine 9 GSK3beta phosphorylation and the enhancement of TCF 4 transcriptional activity . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| The integrin linked kinase ( ILK ) is a cytoplasmic effector of integrin receptors , involved in the regulation of integrin binding properties as well as the activation of cell survival and proliferative pathways , including those involving MAP kinase , PKB / Akt and GSK 3beta . ^^^ By the age of 6 months , female MMTV / ILK mice developed a hyperplastic mammary phenotype , which was accompanied by the constitutive phosphorylation of PKB / Akt , GSK 3beta and MAP kinase . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Integrin linked kinase ( ILK ) is an FA component that interacts with the cytoplasmic domains of integrins , recruits adaptor proteins that link integrins to the actin cytoskeleton , and phosphorylates the serine / threonine kinases PKB / Akt and GSK 3beta . ^^^ However , insulin or PDGF treatment did not impair phosphorylation of PKB / Akt and GSK 3beta , indicating that the proliferation defect is not due to absent or reduced ILK mediated phosphorylation of these substrates in vivo . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Ilk binds the cytoplasmic domain of beta 1 , beta 2 and beta 3 integrins and recruits adaptors and kinases , and is thought to stimulate downstream signalling events through phosphorylation of protein kinase B / Akt ( Pkb / Akt ) and glycogen synthase kinase 3 beta ( GSK 3 beta ) . ^^^ Surprisingly , phosphorylation of Pkb / Akt and GSK 3 beta is unaffected in Ilk deficient chondrocytes . ^^^ These findings suggest that Ilk regulates actin reorganization in chondrocytes and modulates chondrocyte growth independently of phosphorylation of Pkb / Akt and GSK 3 beta . . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Here we report that ILK controls tau phosphorylation via regulation of glycogen synthase kinase 3beta ( GSK 3beta ) activity in N1E 115 cells . ^^^ ILK inactivation resulted in an increase in the active form but a decrease in the inactive form of GSK 3beta , which is a candidate kinase involved in PHF tau formation . ^^^ Moreover , inhibition of GSK 3beta with lithium prevented aberrant tau phosphorylation in the DN ILK expressing cells . ^^^ These results suggest that ILK inactivation results in aberrant tau phosphorylation via sustained activation of GSK 3beta in N1E 115 Cells . ^^^ ILK directly phosphorylates GSK 3beta and inhibits its activity . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Cell adhesion to the extracellular matrix protein fibronectin modulates radiation dependent G 2 phase arrest involving integrin linked kinase ( ILK ) and glycogen synthase kinase 3beta ( GSK 3beta ) in vitro . ^^^ The dependence of cell survival on beta 1 integrin linked kinase ( ILK ) , protein kinase Balpha / Akt ( PKBalpha / Akt ) and glycogen synthase kinase 3beta ( GSK 3beta ) activity , which participate in beta 1 integrin signalling and cell cycle progression was investigated as a function of radiation exposure . ^^^ Cell contact to FN in contrast to polystyrene elevated basal ILK , PKBalpha / Akt and GSK 3beta kinase activities in A 549 and CCD 32 cells , as well as the basal amount of A 549 G2 phase cells . ^^^ These findings suggest a novel pathway that makes direct phosphorylation of GSK 3beta by ILK feasible after irradiation . ^^^ Conclusively , the data indicate that ILK , PKBalpha / Akt and GSK 3beta are involved in modulations of the cell cycle after irradiation . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Inhibition of GSK3beta by LiCl blocks L 6 myogenesis , indicating that ILK mediated inhibition of GSK3beta is not sufficient for differentiation . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Moreover , overexpression of ILK not only coincided with increased MBP phosphotransferase activity but as well with effects on downstream targets like GSK3beta . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| As a function of radiation , the impact of fibronectin ( FN ) and phosphatidylinositol 3 kinase ( PI3K ) related signaling on survival , the cell cycle , and the beta 1 integrin signaling kinases integrin linked kinase ( ILK ) , protein kinase Balpha / Akt ( PKBalpha / Akt ) , and glycogen synthase kinase 3beta ( GSK 3beta ) was examined in normal lung fibroblasts in vitro . ^^^ Colony forming assays , flow cytometric DNA analysis , immunoblotting ( Chk 1 , Chk 2 , Cdc25C , Cdk 1 , 14 3 3 , p 53 , p 21 ) , and protein kinase assays ( ILK , PKBalpha / Akt , GSK 3beta ) were performed with or without PI3K inhibition using LY 294002 or wortmannin . ^^^ The kinase activities of ILK , PKBalpha / Akt , and GSK 3beta were strongly induced by irradiation on polystyrene , but not on FN , which was a result of a FN mediated increase of basal kinase activities . ^^^ In contrast to polystyrene , FN enabled radiation dependent induction of ILK and GSK 3beta in a PI3K independent manner . ^^^ ILK , PKBalpha / Akt , and GSK 3beta involved in integrin signaling were uncovered as new molecular factors within the cellular radiation response . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| We investigated the role of endogenous cellular ILKAP in antagonizing ILK signaling of two key targets , PKB and GSK3beta . ^^^ Consistent with the silencing data , ILKAP inhibition of ILK selectively inhibited S 9 phosphorylation of GSK3beta without affecting S 473 phosphorylation of PKB . ^^^ The expression level of cyclin D 1 , a target of ILK GSK3beta signaling , was inversely correlated with ILKAP protein levels , suggesting that antagonism of ILK modulates cell cycle progression . ^^^ Taken together , our results indicate that endogenous ILKAP activity inhibits the ILK GSK3beta signaling axis , and suggest that ILKAP activity plays an important role in inhibiting oncogenic transformation . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Integrin linked kinase ( ILK ) , bound to the cytoplasmic tails of integrin beta 1 , beta 2 , and beta 3 , is thought to signal through AKT and glycogen synthase kinase 3beta ( GSK 3beta ) for survival and proliferation regulation . ^^^ AKT , GSK 3beta cyclin D 1 , mitogen activated protein kinase kinase 1 / 2 mitogen activated protein kinase , and c Jun NH 2 terminal kinase signaling was dysregulated in irradiated ILK hk mutants . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Interestingly , targeted Ilk deletion has no effect on proliferation or survival of cortical cells or on phosphorylation of two Ilk substrates , Pkb / Akt and Gsk 3beta , suggesting that Ilk does not regulate cortical lamination via these enzymes . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Both ILK and Akt can phosphorylate glycogen synthetase kinase 3beta ( GSK3beta ) , rendering it inactive . ^^^ Mimicking the activation of ILK with specific GSK3beta inhibitors resulted in downregulation of E cadherin levels but had no effect on Cx 32 expression in the MH ( 1 ) C ( 1 ) cells . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Activation of ET ( A ) R by ET 1 triggers an integrin linked kinase ( ILK ) mediated signaling pathway leading to glycogen synthase kinase 3beta ( GSK 3beta ) inhibition , Snail and beta catenin stabilization , and regulation of transcriptional programs that control EMT . ^^^ Transfection of dominant negative ILK or exposure to an ILK inhibitor suppresses the ET 1 induced phosphorylation of GSK 3beta as well as Snail and beta catenin protein stability , activity , and invasiveness , indicating that ET 1 / ET ( A ) R induced EMT promoting effects depend on ILK . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| Increased ILK expression correlated with the activation of PKB / Akt , the phosphorylation of GSK 3beta , and the increased expression of cyclin E and cdk 2 kinase . ^^^ |
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| Interacting proteins: Q13418 and P49841 |
Pubmed |
SVM Score :0.0 |
| This is concomitant with the activation of the integrin linked kinase ( ILK ) pathway , leading to the activation and nuclear translocation of Akt and the inactivation of glycogen synthase kinase 3beta ( GSK3beta ) . ^^^ |
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