| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.58466722 |
| Immunofluorescence microscopy of fibroblasts demonstrated that endogenous ILK as well as transfected green fluorescent protein ILK co localizes with paxillin in focal adhesions . 0.58466722^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| We have recently reported that the paxillin LD 1 motif acts as a binding interface for both the actin binding protein actopaxin and the serine / threonine integrin linked kinase ( ILK ) . ^^^ However , an actopaxin CH 2 domain mutant defective for paxillin binding ( paxillin binding subdomain mutant ) retains the capacity to bind ILK , indicating that paxillin and ILK binding sites on actopaxin are distinct . ^^^ Despite the direct binding between actopaxin and ILK , mutation analysis confirmed a primary role for paxillin in their localization to focal adhesions . ^^^ Interestingly , an ILK mutant ( E359K ) that was previously reported to act as dominant negative for ILK function was unable to bind actopaxin or paxillin and failed to localize to focal adhesions . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| The LD motifs support paxillin binding to actopaxin , integrin linked kinase ( ILK ) , FA kinase ( FAK ) , paxillin kinase linker ( PKL ) and vinculin . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| In salmosin treated BCE cells , focal adhesion kinase ( FAK ) was dephosphorylated and expression of paxillin and p 130 ( CAS ) was decreased , but PI 3 kinase , ILK , and beta catenin were not expressed in decreased amounts or modified , suggesting that salmosin inactivated FAK dependent integrin signaling pathways . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of a mutant ILK lacking kinase activity and / or paxillin binding in ILK deficient fibroblasts can rescue cell spreading , F actin organization , FA formation , and proliferation . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Although many intracellular proteins have been implicated in these processes , a new paradigm is emerging from biochemical and genetic studies that implicates integrin linked kinase ( ILK ) and its interacting proteins , such as CH ILKBP ( alpha parvin ) , paxillin , and PINCH in coupling integrins to the actin cytoskeleton and signaling complexes . ^^^ Here we demonstrate , using several different approaches , that inhibiting ILK kinase activity , or expression , results in the inhibition of cell attachment , cell migration , F actin organization , and the specific cytoskeletal localization of CH ILKBP and paxillin in human cells . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescence stainings of ILK hk cells indicated disturbed ILK and paxillin membrane localization with concomitant decrease in focal adhesions . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| We report here that attenuation of ILK expression in cultured bovine aortic endothelial cells by RNA interference had marked effects on surface distribution of alpha5beta1 integrin and the organization of cell matrix adhesions characterized by the disappearance of fibrillar ( 3D like ) adhesions that are rich in alpha5beta1 and paxillin , and associated fibrillar fibronectin matrix . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Cell lines overexpressing kinase dead ( S343A ) or paxillin binding site mutant ILK proteins display inhibited haptotaxis to fibronectin . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Paxillin delta , which lacks the key phosphorylation sites Y 31 and Y 118 as well as the ILK and actopaxin binding LD 1 motif , exhibits a restricted distribution to epithelial cell types and is downregulated during TGF beta 1 induced EMT of normal murine mammary gland ( NMuMG ) epithelial cells . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Both alpha parvin ( PARVA ) and beta parvin ( PARVB ) localize to focal adhesions and function in cell adhesion , spreading , motility and survival through interactions with partners , such as integrin linked kinase ( ILK ) , paxillin , alpha actinin and testicular kinase 1 . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Taken together with the results that gamma parvin could form a complex with some important cytoskeletal proteins , such as alphaPIX , alpha actinin , and paxillin as demonstrated for affixin and actopaxin ( alpha parvin ) , the results in this study suggest that the ILK gamma parvin complex is critically involved in the initial integrin signaling for leukocyte migration . . ^^^ |
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| Interacting proteins: Q13418 and P49023 |
Pubmed |
SVM Score :0.0 |
| Endogenous ILK was found to associate with PKL ( paxillin kinase linker ) and the Rac / Cdc42 guanine nucleotide exchange factor betaPIX . ^^^ |
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