| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.69932062 |
| Further analyses indicate that ORC 2 physically interacts with ORC 1 on non chromatin nuclear structures . 0.69932062^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| HsORC 1 and HsORC 6 subunits did not enter into this core complex , suggesting that the interaction of these two subunits with the core ORC 2 5 complex is extremely labile . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| A screen based on the two hybrid approach suggests that the product of the ORC 4 gene interacts with the ORC 2 , but not with the ORC 1 protein . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| In Xenopus egg extract , the proteins Orc 1 , Orc 2 , Cdc 6 , and Mcm 4 are underphosphorylated in interphase and hyperphosphorylated in metaphase extract . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| However , this absence of chromatin bound Cdc 6 and MCM proteins from quiescent cells appears not to be due to the absence of ORC subunits as murine homologues of Orc 1 and Orc 2 remain chromatin bound in quiescent cells . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| To investigate the events leading to initiation of DNA replication in mammalian chromosomes , the time when hamster origin recognition complexes ( ORCs ) became functional was related to the time when Orc 1 , Orc 2 and Mcm 3 proteins became stably bound to hamster chromatin . ^^^ Immunoblotting analysis revealed that hamster Orc 1 and Orc 2 proteins were present in nuclei at equivalent concentrations throughout the cell cycle , but only Orc 2 was stably bound to chromatin . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Therefore , unlike the tight stoichiometric association of six yeast ORC subunits in one holo complex , only a small fraction of human ORC 1 and ORC 6 is likely to be associated with a subcomplex of ORC 2 , 3 , 4 , and 5 , suggesting differences in the architecture and regulation of human ORC . . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Finally , all ORC subunits remain consistently nuclear , and Orc 2 is consistently phosphorylated through all stages of the cell cycle , whereas Orc 1 is selectively phosphorylated in mitosis . . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Since the concentration of Orc 1 remains constant throughout the cell cycle , and its half life in vivo is the same as that of Orc 2 , ubiquitination of non chromatin bound Orc 1 presumably facilitates the inactivation of ORCs by sequestering Orc 1 during S phase . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| For this purpose , we performed chromatin immunoprecipitation assays with antibodies against human Orc 1 and Orc 2 proteins . ^^^ Both , Orc 1 and Orc 2 proteins are found at the intergenic region in the G ( 1 ) phase , but S phase chromatin contains only Orc 2 protein , supporting the notion that Orc1p dissociates from its binding site in the S phase . ^^^ Sequences corresponding to the intergenic region are highly abundant in a fraction of nascent DNA strands , strongly suggesting that this region not only harbors the binding sites for Orc 1 protein and Orc 2 protein but also serves as an origin of bidirectional DNA replication . . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Cdc 23 also interacts with four subunits of the Schizosaccharomyces pombe origin recognition complex ( ORC ) in yeast two hybrid assay : Orc 1 , Orc 2 , Orc 5 and Orc 6 . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| In plants , however , the only cloned putative homologs of ORC subunits are the Arabidopsis ORC 2 and the rice ORC 1 . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| In particular CDC 6 was found to bind strongly to ORC 1 and ORC 2 , and to MCM 7 proteins . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Two isoforms of the human ornithine carrier , ORC 1 and ORC 2 , have been identified by overexpression of the proteins in bacteria and by study of the transport properties of the purified proteins reconstituted into liposomes . ^^^ ORC 2 has a broader specificity than ORC 1 , and L and D histidine , L homoarginine , and D isomers of ornithine , lysine , and ornithine are all substrates . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| An artificial reduction in the level of ORC 1 in human cells by RNA interference results in a shift of ORC 2 to the nuclease soluble fraction , and the association of MCM proteins with chromatin fractions is also blocked by this treatment . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| In G ( 1 ) , the pre replicative complex contains CDC 6 , MCM 3 , ORC 1 and ORC 2 proteins ; of these , the post replicative complex in S phase contains only ORC 2 ; in M phase none of them are bound . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitations and quantitative polymerase chain reaction analysis revealed that , in G 1 phase cells , Orc 1 and Orc 2 , as well as Mcm 3 , another component of the prereplication complex , are bound to multiple sites on the plasmid . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| However , chromatin binding was not mediated through increased affinity of Orc 1 for Orc 2 , suggesting that additional events are involved in the assembly of functional ORC chromatin sites . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Sequence analysis suggests the existence of at least two subfamilies of these proteins , for which we propose the nomenclature ORC 1 and ORC 2 . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| DNA affinity purification confirmed that LANA , CBP , BRD 2 , and ORC 2 bound TR specifically and identified the histone acetyltransferase HBO 1 ( histone acetyltransferase binding to ORC 1 ) as a potential TR binding protein . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| To define the physiological functions of the mammalian ORC , we cloned ORC subunit cDNAs from mouse NIH3T3 cells and found novel variant forms of Orc 1 , Orc 2 , and Orc 3 each derived from alternative RNA splicing . ^^^ The variant form of Orc 1 , Orc1B , lacks 35 amino acid residues in exon 5 ; the variant of Orc 2 , Orc2B , lacks 48 amino acid residues in exon 2 . ^^^ Taken together , we conclude that mouse Orc 1 , Orc 2 , and Orc 3 each exist in two alternative splicing variants and that naturally occurring Orc1B lacks a functional domain that is essential for nuclear translocation and proteasome dependent degradation . . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| We demonstrated the utility of this system by showing the functional importance of the ATPase domains of human Cdc 6 and Orc 1 and the dispensability of the N terminal segments of Orc 1 and Orc 2 in this assay . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Ubiquitylation , phosphorylation and Orc 2 modulate the subcellular location of Orc 1 and prevent it from inducing apoptosis . ^^^ Remarkably , expression of Orc 1 in the presence of an equivalent amount of Orc 2 , the only ORC subunit that did not induce apoptosis , prevented induction of apoptosis and restored uniform nuclear localization of Orc 1 . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| To study the function of the ORC during defined cell cycle periods , we performed cell cycle execution point analyses with strains containing a conditional mutation in the ORC 1 , ORC 2 or ORC 5 subunit of ORC . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| The binding of the prereplication complex proteins Orc 1 , Orc 2 , Mcm 3 , Mcm 7 , and Cdc 6 and the novel DNA unwinding element ( DUE ) binding protein DUE B to the endogenous human c myc replicator was studied by chromatin immunoprecipitation . ^^^ In G ( 1 ) arrested HeLa cells , Mcm 3 , Mcm 7 , and DUE B were prominent near the DUE , while Orc 1 and Orc 2 were least abundant near the DUE and more abundant at flanking sites . ^^^ Similarly , the signals from Orc 1 , Mcm 3 , and Mcm 7 were at background levels in cells arrested in M phase , whereas Orc 2 retained the distribution seen in G ( 1 ) phase cells . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| Using the yeast two hybrid system and immunoprecipitation , we report here that murine HP1alpha interacts with the preRC proteins ORC 1 , ORC 2 and CDC 6 . ^^^ Immunofluorescence staining and EGFP / DsRed fusion proteins revealed a colocalization of HP1alpha with ORC 1 , ORC 2 and CDC 6 in heterochromatin , supporting the notion that ORC and probably CDC 6 play an important role in murine HP1alpha function . ^^^ |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q13416 |
Pubmed |
SVM Score :0.0 |
| NA |
|