| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.76153397 |
| Co immunoprecipitation revealed that Cdc 42 was bound to PLD 1 during differentiation , whereas RhoA was associated with PLD 1 during both proliferation and differentiation . 0.76153397^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Purified hPLD 1 is activated by several cytosolic cofactors among which are protein kinase Calpha , ARF , and RhoA . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Interaction of the small G protein RhoA with the C terminus of human phospholipase D 1 . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Cav 3 enriched light membrane ( CELM ) fractions obtained from C2C12 myotubes contain phospholipase D 1 ( PLD 1 ) and its major regulators , RhoA and protein kinase Calpha ( PKCalpha ) . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Determination of interaction sites of phospholipase D 1 for RhoA . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Activation of phospholipase D 1 by ADP ribosylated RhoA . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of phospholipase D 1 and the modulation of its interaction with RhoA by cAMP dependent protein kinase . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| RhoA mediated Phospholipase D 1 signaling is not required for the formation of stress fibers and focal adhesions . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| PLD 1 , first cloned from human , is activated by small GTPases such as ADP ribosylation factor ( ARF ) and RhoA . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| The DIF contained PLD 1 , RhoA , and ARF , and the level of each was increased by GTPgammaS in a dose dependent manner . ^^^ These results indicate that stimulation of PLD 1 is accompanied by stable association of the activated lipase , RhoA , and ADP ribosylation factor with the actin based membrane skeleton . . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| We show that the amino terminal 325 amino acids are required for PKC alpha activation of PLD 1 but not for activation by ARF 1 and RhoA . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Whereas both PLD enzymes are dependent on phosphatidylinositol 4 , 5 bisphosphate for activity only the PLD 1 isoform was strongly stimulated by the small GTPases ARF and RhoA and by protein kinase Calpha as well . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| PLD 1 is activated by small molecular weight G proteins , such as ADP ribosylation factor ( ARF ) and RhoA family members , while PLD 2 is relatively independent of such stimuli . ^^^ To establish the dependence of PLD 1 on small molecular weight G proteins , the translocations of ARF and RhoA to the membrane fractions was determined after stimulation by TSH . ^^^ It is concluded that TSH stimulates PLD 1 activity in FRTL 5 thyroid cells and this is accompanied by the translocation of ARF and RhoA to the membrane fraction . . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| We have used the yeast split hybrid system to generate PLD 1 alleles that fail to bind to or to be activated by RhoA but that retain wild type responses to ARF and PKC . ^^^ Our results demonstrate that direct stimulation of PLD 1 in vivo by RhoA ( and by PKC ) is critical for significant PLD 1 activation but that PLD 1 subcellular localization and regulated phosphorylation occur independently of these stimulatory pathways . . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Catalytically inactive constructs of PLD 2 and RhoA , but not PLD 1 , ADP ribosylation factor 1 ( ARF 1 ) , ARF 6 , or ADP ribosylation factor nucleotide exchange factor ( ARNO ) blocked Ang 2 induced PLD activity in SHR and WKY PGSMCs . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| These effects have been related to preventing activation by ARF 1 , RhoA , and protein kinase C alpha and beta and therefore indicate that PLD 1 is inhibited . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| While a potential breakdown product of PLD 1 , activation by RhoA indicates that the PLD characterized here is distinct from the other PLDs cloned or isolated to date . . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| To understand the mechanism of the insert mediated activation of PLD 1 by Cdc 42 and to develop reagents to study Cdc 42 activated PLD 1 in cellular signaling events , we have undertaken a mutational analysis of the Rho insert region of Cdc 42 and examined the specificity of the insert helix requirement in the other Rho family members , RhoA and Rac 1 . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Effects of both agonists on the nuclear fraction , which contains only PLD 1 , are compatible with a RhoA and PKCdelta dependent process . ^^^ In membranes , which contain both PLD 1 and PLD 2 , the stimulatory effect of S1P on PLD activity can best be explained by RhoA and PKCdelta dependent activation of PLD 1 ; in contrast , the effects of BK on RhoA translocation and enhancement of BK stimulated PLD activity by PKC inhibition are both consistent with PLD 2 serving as its primary target . . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| In an established cardiac myocyte model of preconditioning using the cultured chick embryo heart cells , overexpression of the RhoA noninteracting PLD 1 mutant I870R selectively blocked the A 3 agonist ( Cl IBMECA , 10 nM ) induced cardioprotection . ^^^ To further test the anti ischemic role of a direct RhoA PLD 1 interaction , atrial cardiac myocytes were rendered null for native adenosine receptors by treatment with irreversible A 1 antagonist m DITC XAC and were selectively transfected with the human adenosine A 1 or A 3 receptor cDNA individually or they were cotransfected with cDNAs encoding either receptor plus I870R . ^^^ The RhoA noninteracting PLD 1 mutant caused a significantly higher percentage of cardiac cells killed in myocytes cotransfected with the human A 3 receptor than in those cells expressing the human A 1 receptor ( ANOVA and posttest comparison , P < 0 . 01 ) . ^^^ The present data provided the first demonstration of a novel physiological role for the direct RhoA PLD 1 interaction , that of potent protection from cardiac ischemia . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| Retinoic acid ( RA ) induced differentiation increased PLD 1 and PLD 2 mRNA levels and PLD activity that was responsive to phorbol myristate acetate . ^^^ |
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| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| We also show that LPA induced nuclear PLD 1 activation implied PI3K / PKCzeta pathway activation and PKCzeta nuclear translocation as well as nuclear RhoA activation . ^^^ |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13393 and P61586 |
Pubmed |
SVM Score :0.0 |
| NA |
|