Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.67319718
Ucn binds with very high affinity to both CRF receptor type 1 ( CRF R 1 ) and CRF receptor type 2 ( CRF R 2 ) . 0.67319718^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.6274689
Corticotropin releasing hormone ( CRH ) and urocortins ( Ucn ) bind with various affinities to two G protein coupled receptors , CRHR 1 and CRHR 2 , which are expressed in brain and in peripheral tissues , including immune cells . 0.6274689^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Male Sprague Dawley rats were treated with antisense and sense oligonucleotides ( ON ) to CRF 2 receptor mRNAs for 36 h and then received an intracerebroventricular ( i . c . v . ) injection of CRF , UCN ( 3 micrograms ) or saline . ^^^ The data suggest that down regulation of CRF 2 receptors selectively attenuates CRF and UCN induced anorexia and hypothalamo pituitary adrenocortical activation in rats . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
A recently cloned related peptide , Urocortin ( Ucn ) , appears to have similar affinity for the CRF 1 receptor , but higher affinity at the CRF 2 receptor . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The anorectic effects of CRF and UCN were not blocked by the CRF 1 receptor antagonist antalarmin , suggesting that the peripheral effects of CRF and UCN on food intake are mediated primarily by the CRF 2 receptor . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
In the CeA , CRF ( r / h ) depressed excitatory glutamatergic transmission through a CRF 1 mediated postsynaptic action , whereas Ucn 1 facilitated synaptic responses through presynaptic and postsynaptic CRF 2 mediated mechanisms . ^^^ Conversely , in the LSMLN , CRF caused a CRF 1 mediated facilitation of glutamatergic transmission via postsynaptic mechanisms , whereas Ucn 1 depressed EPSCs by postsynaptic and presynaptic CRF 2 mediated actions . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin 2 ( Ucn 2 ) , a CRF related peptide that selectively binds CRF 2 receptors , was recently identified and recognized for its central anorectic properties . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin 2 ( Ucn 2 ) and Ucn 3 are new members of the corticotropin releasing factor ( CRF ) family and bind selectively to the CRF type 2 receptor ( CRF 2 ) . ^^^ These results suggest that the CRF 2 receptor is involved in motor suppressive effects as well as anxiolytic and anorectic effects of Ucn 2 and Ucn 3 . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
In addition to urocortin ( Ucn 1 ) , Ucn 2 and Ucn 3 were identified as endogenous ligands for corticotropin releasing factor type 2 receptor ( CRF 2 receptor ) . ^^^ Therefore , it is thought that Ucn 2 may modulate CRF and vasopressin synthesis in the PVN in a paracrine or autocrine fashion through PVN CRF 2 receptor . ^^^ The early and later phases of Ucn 1 mediated feeding suppression may be CRF 1 and CRF 2 receptor mediated events , respectively . ^^^ The systemic administration of Ucn 2 decreases mean arterial pressure ( arterial vascular tone ) and causes tachycardia via vascular CRF 2 receptor in rats , similar to the effects of Ucn 1 . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin 1 binds with high affinity to CRF type 1 ( CRF 1 ) and type 2 ( CRF 2 ) receptors while Ucn 2 and Ucn 3 are selective CRF 2 ligands . ^^^ In addition , Ucn 2 showed visceral anti nociceptive activity associated with the selective activation of CRF 2 receptors . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The intra DRN administration of the selective CRF 2 agonist urocortin 2 ( Ucn 2 ) dose dependently increased 5 HT efflux in the basolateral amygdala , a projection region of the DRN . ^^^ Both of these effects of Ucn 2 were completely blocked by intra DRN antisauvagine 30 ( ASV 30 ) , a relatively selective CRF 2 antagonist . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Endogenous agonists ( urocortin ( UCN ) 2 , UCN 3 ) and synthetic antagonists ( astressin 2 B , antisauvagine 30 ) are selective for CRF 2 over the CRF 1 receptor . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Corticotropin releasing factor ( CRF ) and urocortin ( Ucn 1 ) are endogenous members among a family of CRF related peptides that activate two different and synaptically localized G protein coupled receptors , CRF 1 and CRF 2 . ^^^ In brain slice recordings from the LSMLN of control ( saline treated ) rats , glutamatergic synaptic transmission was facilitated by activation of CRF 1 receptors with CRF but was depressed after activation of CRF 2 receptors with Ucn 1 . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Ucn 2 is expressed in the adrenal medulla , and its receptor , CRF 2 receptor , is also expressed in the adrenal gland . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Ucn 1 binds to both CRF type 1 ( CRF 1 ) and type 2 receptors ( CRF 2 ) , whereas Ucn 3 is a specific agonist for CRF 2 . ^^^ Immunohistochemistry in serial tissue sections with mirror images revealed that both Ucn 3 and CRF 2 were colocalized in more than 85 % of the adrenocortical cells . mRNA in situ hybridization confirmed these findings above . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin 3 ( Ucn 3 ) or stresscopin ( SCP ) is a new member of the corticotropin releasing factor ( CRF ) neuropeptide family and is a specific ligand for CRF type 2 receptor ( CRF 2 ) . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin 1 ( UCN 1 ) , a peptide of the CRF family , binds both CRF 1 receptor and CRF 2 receptor with equal affinities . ^^^ Urocortin 3 ( UCN 3 ) , a highly selective ligand for CRF 2 receptor with little affinity for CRF 1 receptor , has been shown in rat pancreatic beta cells . ^^^ Antisauvagine 30 , a selective CRF 2 receptor antagonist , appeared to enhance the UCN 1 effect on the elevation of [ Ca2+ ] 1 . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Ucn activates CRF receptors ( both CRF R 1 and CRF R 2 ) with greater potency than CRF itself , suggesting that Ucn may play an endogenous role in eliciting ( at least some ) CRF receptor mediated events . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
In the gastrointestinal tract , these compounds open new therapeutic options in the treatment of lower GI inflammatory diseases associated to CRF , such as the chronic inflammatory bowel syndromes , irritable bowel disease and ulcerative colitis while Ucn , Ucn 1 , Ucn 2 or synthetic non peptide CRF 2 agonists may be useful in the treatment of upper GI inflammatory diseases . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
In vivo studies suggest that corticotrophin releasing factor ( CRF ) and CRF like peptides , urocortin 1 ( UCN 1 ) and UCN 2 , inhibit gastric emptying and stimulate colonic motility through CRF 2 and CRF 1 receptors , respectively . ^^^ We evaluated expression and functions of CRF , UCN 1 , UCN 2 and CRF 1 and CRF 2 receptors in the rat gastric antrum . ^^^ Quantitative RT PCR demonstrated UCN 2 , CRF 1 and CRF 2 receptors expressions in both muscle layers and mucosa of the gastric antrum . ^^^ These results demonstrate ( 1 ) the presence of CRF , UCN and CRF 1 and CRF 2 receptors in the rat gastric antrum ; ( 2 ) that , in vitro , CRF like peptides inhibit phasic contractions of the antrum through CRF 2 receptor . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
To determine if the CRF 2 receptor agonist urocortin 2 ( Ucn 2 ) increases c Fos expression in rat DR serotonergic neurons via actions on CRF 2 receptors , we gave intracerebroventricular ( icv ) injections of mouse Ucn 2 after icv injections of either saline or the CRF 2 receptor antagonist antisauvagine 30 ( ASV 30 ) . ^^^ Mouse Ucn 2 had no effect on c Fos expression within the median raphe nucleus , consistent with the hypothesis that Ucn 2 has specific actions on an anatomically and functionally distinct subset of serotonergic neurons via activation of CRF 2 receptors . ^^^ These findings are also consistent with the hypothesis that Ucn 2 , or another CRF related neuropeptide acting at CRF 2 receptors , modulates physiological and behavioral responses to stress related stimuli via actions on a specific subset of serotonergic neurons within the dorsal raphe nucleus . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin ( UCN ) , is a peptide related to hypothalamic corticotrophin releasing hormone ( CRF ) and binds with high affinity to the CRF R 2 beta receptor which is expressed in the heart . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Pharmacological studies showed that Ucn 3 is a high affinity ligand for the type 2 CRF receptor ( CRF R 2 ) . ^^^ Several major Ucn 3 terminal fields identified in the present study , including the lateral septum and the ventromedial hypothalamus , are known to express high levels of CRF R 2 . ^^^ Thus , these anatomical data strongly support the notion that Ucn 3 is an endogenous ligand for CRF R 2 in these areas . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Histochemical and axonal transport methods were used to clarify the central organization of cells and fibers that express urocortin ( UCN ) , a recently discovered corticotropin releasing factor ( CRF ) related neuropeptide , which has been proposed as an endogenous ligand for type 2 CRF receptors ( CRF R 2 ) . ^^^ Although substantial UCN ir projections were observed to several brainstem cell groups that express CRF R 2 , including the dorsal raphe and interpeduncular nuclei and the nucleus of the solitary tract ( NTS ) , most prominent seats of CRF R 2 expression were found to contain inputs immunopositive for piscine urotensin 1 , but not rat UCN . ^^^ Several observations , including the lack of a pervasive relationship of UCN ir projections with CRF R 2 expressing targets , support the existence of still additional CRF related peptides in mammalian brain . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Unique sites of CRF R 2 expression were relatively unresponsive to CRF but were more so after icv administration of urocortin ( UCN ) , a new mammalian CRF related peptide . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Given the multiple immunomodulatory effects of peripherally administered CRF and UCN and the high levels of CRF receptor type 2 ( CRF R 2 ) mRNA and protein expressed in the heart , the lymphoid organs and heart have become targets for some of the latest CRF related research . ^^^ Following on from these initial results , we used semi quantitative RT PCR to carry out a comprehensive study assessing the relative amounts of CRF , UCN , CRF R 1 , CRF R 2 and CRF BP mRNAs in the brain , thymus , spleen and heart of normal , untreated rats . ^^^ The rank orders of mRNA abundance in each of the tissue types were as follows : for CRF , brain > > thymus=spleen=heart ; for UCN , heart > / =brain > thymus > spleen ; for CRFR 1 , brain > > thymus > spleen ( absent in heart ) ; for CRF R 2 , brain=heart > thymus > spleen ; and CRF BP was only detectable in the brain . ^^^ We have provided evidence for the existence of CRF , UCN , CRF R 1 and CRF R 2 expression in resting immune cells , with UCN expression being particularly predominant in the rat thymus and human Jurkat cells . ^^^ Additionally , the high levels of UCN mRNA detected in heart corresponded to the high expression of CRF R 2 mRNA , suggesting an important role for UCN / CRF R 2 coupling in this tissue . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The distinctive expression pattern of CRF R 2 mRNA identifies additional putative central sites of action for CRF and / or UCN . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Immediately after the second 3 h bout of restraint stress , there was a significant increase in expression of UCN in the Edinger Westphal nucleus and of CRF R 1 in the paraventricular nucleus of the hypothalamus and a less pronounced decrease in CRF R 2 expression in the ventromedial nucleus of the hypothalamus . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The human endometrium expresses both UCN and CRF , and CRF / UCN receptors type 1 ( CRF R 1 ) and 2 ( CRF R 2 ) . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
CRF R 1 has higher affinity for CRF than does CRF R 2 while both receptors bind Ucn 1 equally . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
In this study , we examined the effects of CRH deficiency and / or stress due to physically restraint or lipopolysaccharide ( LPS ) administration on expression of transcripts for CRH R 2 ( CRH R 2 mRNA ) as well as urocortin ( UCN ) mRNA in the atria and ventricle using female and male CRH deficient ( knockout , KO ) mice . ^^^ CRH deficiency caused a robust decrease in basal levels of CRH R 2 mRNA and a significant increase of UCN mRNA expression in the atria and ventricle of female as well as male mice . ^^^ Restraint stress markedly reduced CRH R 2 mRNA and increased UCN mRNA expression on atria as well as ventricle in both female and male wild type ( WT ) mice . ^^^ Following LPS injection to both female and male mice , CRH R 2 mRNA expression was decreased and UCN mRNA expression was increased in the atria and ventricle of both WT and CRH KO mice in each sex . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Using RT PCR and fluorescent in situ hybridization , we demonstrated that UCN 2 and type 2 CRH receptor ( CRH R 2 ) mRNAs were expressed in human nonpregnant and pregnant myometrium . ^^^ Immunofluorescent studies confirmed protein expression of UCN 2 in human pregnant myometrial cells , whereas chemical cross linking studies with radiolabeled UCN 2 confirmed the presence of CRH R 2 sites with an apparent molecular mass of 50 kDa . ^^^ Treatment of primary human myometrial cells with UCN 2 to specifically activate CRH R 2 resulted in a dose dependent increase of myosin light chain ( MLC ( 20 ) ) phosphorylation . ^^^ Collectively , our data suggest a distinctive role for CRH R 2 specific agonists like UCN 2 in the control of myometrial contractility during human pregnancy involving sequential activation of PKC , MAPK kinase 1 , ERK1 / 2 , RhoA , and RhoA associated kinase , leading to the MLC ( 20 ) phosphorylation . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The two main mammalian peptides of this family , CRH and urocortin ( UCN ) , signal through the same receptors , CRH R 1 and CRH R 2 . ^^^ Recent studies conducted on mice deficient in CRH , CRH R 1 , CRH R 2 and CRH R1 / CRH R 2 , as well as in two different UCN deficient mice , have added relevant information towards the understanding of the role of this peptide family in the CNS . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin ( Ucn ) 2 and 3 , homologous peptides of Ucn that are specific ligands for corticotropin releasing hormone ( CRH ) type 2 receptor ( CRH R 2 ) , have recently been identified . ^^^ The effects of Ucn 2 were attenuated by astressin 2 B , a specific antagonist of CRH R 2 , and / or H 89 , an inhibitor of protein kinase A ( PKA ) . ^^^ These results indicate that Ucn 2 may be another endogenous cardiovascular substance that acts via CRH R 2 and the cAMP dependent PKA pathway . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
CRFR 1 and CRFR 2 , the receptors for CRF and Ucn , are expressed in neurons associated with appetite control and metabolism , but their relative contributions in mediating CRF or Ucn induced hypophagia and weight loss are not known . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin ( Ucn ) 3 , or stresscopin , is a high affinity ligand for the type 2 corticotropin releasing factor ( CRFR 2 ) receptor recently identified in rodents and human . ^^^ The observed effects of Ucn 3 were blocked by pretreatment with a CRFR 2 antagonist , astressin ( 2 ) B . ^^^ Furthermore , Ucn 3 stimulated glucagon and insulin release from isolated rat islets , and astressin ( 2 ) B abolished the effects of Ucn 3 , in keeping with a CRFR 2 mediated mechanism . ^^^ Taken together , the present studies suggest pancreatic Ucn 3 acting through CRFR 2 is involved in the local regulation of glucagon and insulin secretion . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
UCN is able to bind to two types of G protein coupled receptors : CRF receptor type 1 ( CRFR 1 ) and CRF receptor type 2 ( CRFR 2 ) , whereas , two homologues of UCN , stresscopin ( SCP ) or also known as urocortin 3 ( UCNIII ) and stresscopin related peptide ( SRP ) , or urocortin 2 ( UCNII ) , bind exclusively and with high affinity to CRFR 2 , we hypothesised that they will exhibit more pronounced cardioprotective effects than UCN . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Studies of the regulation of ERK1 / 2 p 42 , 44 by Ucn 1 were extended to cell lines that endogenously express CRFR 1 ( AtT 20 and CATHa cells ) and CRFR 2 ( A7r5 and CATHa cells ) . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The CRFR 2 selective antagonist astressin 2 B and an ERK1 / 2 p 42 , 44 inhibitor abolish the cardioprotective actions of Ucn 2 and Ucn 3 in reperfusion . ^^^ We propose the use of CRFR 2 selective agonists , Ucn 2 and Ucn 3 , to treat ischemic heart disease because of their potent cardioprotective effects in the murine heart and their minimal impact on the hypothalamic stress axis . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
We found a significant increase in Ucn 2 mRNA levels in the skin , but not in skeletal muscle , of both CRFR 1 and CRFR 2 null mice compared with their wild type littermates . ^^^ Further examination of the distribution and regulation of CRFR 2 and its specific ligand Ucn 2 in the skin and skeletal muscle tissues may reveal the manner by which the CRFR 2 pathway is involved in the physiological responses to stress in these tissues and in other pathophysiologies of the skin and muscle . . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Urocortin ( Ucn ) , a Crh related neuropeptide and the postulated endogenous ligand for Crhr 2 , is a potential mediator of stress responses . ^^^ Unlike Crhr 1 or Crhr 2 deficient mice , Ucn deficient mice exhibit normal anxiety like behavior as well as autonomic regulation in response to stress . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
In addition , high doses of CRH are required to produce LH , which is known to be mediated by 5 HT excitation , and the CRHR 2 agonist urocortin 2 ( UCN 2 ) produces LH at much lower doses than does CRH . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
CRH mRNA levels did not change during the luteal phase , whereas expression of UCN , UCN 2 , CRHR 1 , and CRHR 2 was maximal at early or mid luteal phase before declining ( P < 0 . 05 ) at the later stages . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The UCN homologues SCP and SRP bind specifically to the CRFR 2 receptor , whereas UCN binds to both CRFR 1 and CRFR 2 . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Here we show in the Sprague Dawley rat that direct in vivo application to the ventromedial hypothalamus ( VMH ) , a key glucose sensing region , of urocortin 1 ( UCN 1 ) , an endogenous CRFR 2 agonist , suppressed ( approximately 55 60 % ) , whereas CRF , a predominantly CRFR 1 agonist , amplified ( approximately 50 70 % ) CRR to hypoglycemia . ^^^ Interestingly , the suppressive effect of UCN 1 mediated CRFR 2 activation persisted for at least 24 hours after in vivo VMH microinjection . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The actions of corticotropin releasing hormone ( Crh ) , a mediator of endocrine and behavioural responses to stress , and the related hormone urocortin ( Ucn ) are coordinated by two receptors , Crhr 1 ( encoded by Crhr ) and Crhr 2 . ^^^ We also demonstrate that Crhr 2 is essential for sustained feeding suppression ( hypophagia ) induced by Ucn . ^^^ Feeding is initially suppressed in Crhr 2 / mice following Ucn , but Crhr 2 / mice recover more rapidly and completely than do wild type mice . ^^^ In addition to central nervous system effects , we found that , in contrast to wild type mice , Crhr 2 / mice fail to show the enhanced cardiac performance or reduced blood pressure associated with systemic Ucn , suggesting that Crhr 2 mediates these peripheral haemodynamic effects . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
Crh has a higher affinity for Crhr 1 than for Crhr 2 , and urocortin ( Ucn ) , a Crh related peptide , is thought to be the endogenous ligand for Crhr 2 because it binds with almost 40 fold higher affinity than does Crh . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
The intermediate portion of the lateral septum ( LSi ) contains high levels of urocortin ( UCN ) peptide and type 2 corticotropin releasing hormone ( CRH ) receptor ( CRHR 2 ) and has anatomic and functional connections with the lateral hypothalamus ( LH ) . ^^^ In conclusion , UCN significantly inhibits feeding induced by deprivation and LH injected orexin A without producing a CTA , an effect that is mediated by CRHR 2 . ^^^
Interacting proteins: Q13324 and P55089 Pubmed SVM Score :0.0
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