| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Natural born killer ( NBK ) , also known as Bcl 2 interacting killer ( BIK ) , is a death promoting Bcl 2 family protein sharing with Bcl 2 only the Bcl homology 3 ( BH 3 ) domain . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| We observed a general up regulation of positive proliferation regulators , including survivin , multiple growth factors , genes involved in cell cycle progression , and regulatory factor receptor loops , and the down regulation of transcriptional repressors , such as Mad 4 and JunB , and of antiproliferative and proapoptotic genes , including B cell translocation gene 1 and 2 , cyclin G 2 , BCL 2 antagonist / killer 1 , BCL 2 interacting killer , caspase 9 , and TGFbeta family growth inhibitory factors . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| The proapoptotic BH 3 only protein natural born killer / Bcl 2 interacting killer ( Nbk / Bik ) has been described to inhibit Bcl 2 and Bcl xL , thereby supporting the death promoting ability of Bax . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| In detail , we show that loss of the BH 3 only protein natural born killer ( Nbk ) / Bcl 2 interacting killer ( Bik ) is a common feature of clear cell renal cell carcinoma ( RCC ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bik , a novel death inducing protein shares a distinct sequence motif with Bcl 2 family proteins and interacts with viral and cellular survival promoting proteins . ^^^ We have identified a novel cellular protein , Bik , that interacts with the cellular survival promoting proteins , Bcl 2 and Bcl xL , as well as the viral survival promoting proteins , Epstein Barr virus BHRF 1 and adenovirus E1B 19 kDa . ^^^ In transient transfection assays , Bik promotes cell death in a manner similar to the death promoting members of the Bcl 2 family , Bax and Bak . ^^^ This death promoting activity of Bik can be suppressed by coexpression of Bcl 2 , Bcl XL , EBV BHRF 1 and E1B 19 kDa proteins suggesting that Bik may be a common target for both cellular and viral anti apoptotic proteins . ^^^ While Bik does not show overt homology to the BH 1 and BH 2 conserved domains characteristic of the Bcl 2 family , it does share a 9 amino acid domain ( BH 3 ) with Bax and Bak which may be a critical determinant for the death promoting activity of these proteins . . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Since the discovery of Bcl 2 a decade ago , several other cellular and viral genes encoding homologous proteins have been identified , some of which suppress cell death akin to Bcl 2 ( Bcl XL , Mcl 1 , A1 / Bfl 1 , Nr 13 , Ced 9 , BHRF 1 ) and others which promote apoptosis ( Bax , Bcl Xs , Bak , Bik , Bad ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Mutation of a tyrosine residue within the conserved N terminal BH 4 region had no effect on the ability of Bcl 2 or its closest homologs to enhance cell survival and did not prevent heterodimerization with death enhancing family members Bax , Bak , Bad and Bik . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Recent studies have focused on the role that programmed cell death ( i . e . , apoptosis ) plays in both normal and neoplastic growth : certain genes can either suppress ( e . g . , Bcl 2 , Bcl xL ) or promote ( e . g . , Bik , Bax , Bak ) apoptosis . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| In addition , TGF beta transcriptionally regulates Bcl 2 family members , Bcl XL and Bik , to further influence the apoptosis process . . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| We suggest that the anti apoptotic effect of Bcl 2 is related to its ability to maintain a threshold level of Ca ( m ) and DeltaPsi ( m ) while the pro apoptotic protein Bik has the opposite effect . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| In the yeast two hybrid system , Mcl 1 binds to the hypophosphorylated mutant of BAD and interacts preferentially with different proapoptotic ( Bax , Bak , Bok , Bik , and BOD ) compared with antiapoptotic Bcl 2 family members ( Bcl 2 , Bcl xL , Bcl w , Bfl 1 , CED 9 , and BHRF 1 ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Using isolated mitochondria , we showed that Bid and Bik , BH 3 only proteins from the Bcl 2 family , induced cytochrome c release but not Deltapsi loss . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| IRS 2 but not IRS 3 binds to Bcl 2 , and IRS 1 associates with Bcl XL but not with Bax or Bik . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bik is a potent pro apoptotic protein , which complexes with various anti apoptotic proteins such as Bcl 2 , Bcl xL , 19 kDa adenovirus E1B , and EBV BHRF 1 . ^^^ This result is consistent with the hypothesis that Bik may promote cell death by complexing with and antagonizing the activity of endogenous cellular anti apoptosis proteins such as Bcl 2 and Bcl xL . ^^^ To elucidate the relationship between protein complex formation and induction of cell death , we have identified the minimal sequences of Bik , from a library of N terminal and C terminal deletion mutants , required for interaction with Bcl 2 and Bcl xL and for inducing efficient cell death . ^^^ Two hybrid analysis in yeast and immunoprecipitation analysis of proteins expressed in mammalian cells indicate that a 52 amino acid region ( amino acids 43 94 ) of Bik , encompassing the BH 3 domain , is sufficient for efficient heterodimerization with Bcl 2 and Bcl xL . ^^^ Functional analysis indicates that a Bik deletion mutant expressing residues 43 120 , which efficiently heterodimerizes with the anti apoptosis proteins Bcl 2 and Bcl xL , is defective in eliciting cell death . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Deletion of BH 4 rendered Bcl 2 ( and Bcl xL ) inactive but did not impair either Bcl 2 homodimerization or ability to bind to Bax or five other pro apoptotic relatives ( Bak , Bad , Bik , Bid or Bim ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| The EGL 1 protein contains a nine amino acid region similar to the Bcl 2 homology region 3 ( BH 3 ) domain but does not contain a BH 1 , BH 2 , or BH 4 domain , suggesting that EGL 1 may be a member of a family of cell death activators that includes the mammalian proteins Bik , Bid , Harakiri , and Bad . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| GnRH Bik / Bax / Bak chimeric proteins target and kill adenocarcinoma cells ; the general use of pro apoptotic proteins of the Bcl 2 family as novel killing components of targeting chimeric proteins . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| No changes in the expression of Bax , Bid and Bik ( pro apoptotic proteins ) , or Bcl 2 ( an anti apoptotic protein ) were detected . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| A DNA microarray analysis identified the BH 3 only BCL 2 family member , BIK / NBK , as a transcript that is upregulated during induction of apoptosis by oncogenic E1A . ^^^ Elevated BCL 2 functioned downstream of p 53 and BIK induction to inhibit the E1A death pathway , with the ratio of anti apoptotic BCL 2 and pro apoptotic BIK determining cell death or survival in E1A expressing cells . ^^^ Cells expressing BCL 2 or treated with the pan caspase inhibitor , zVAD fmk , allowed accumulation of high levels of cytotoxic BIK compared to control cells . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation state of antiapoptotic ( Bcl 2 , Bcl 10 ( L ) ) and proapoptotic ( BAD , Bid , Bik ) Bcl 2 proteins regulates their cellular activity and , therefore , cell survival and cell death . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Another subset characterized by the BH 3 peptides from BAD and BIK can not directly activate BAX , BAK but instead binds antiapoptotic BCL 2 , resulting in the displacement of BID like BH 3 domains that initiate mitochondrial dysfunction . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| The Bcl 2 homology 3 ( BH 3 ) domain is present in most members of the Bcl 2 protein family and is required to confer the death inducing properties of pro apoptotic members , including Bax , Bak , Bad , and Bik , in cell based assay systems . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Using reverse transcriptase polymerase chain reaction , cloning , and sequencing techniques , we have found that HL 60 cells express bak , bik , bax , bad , bcl 2 , bcl xL , bcl w , bfl 1 , fas , and caspases 1 4 and 7 10 . ^^^ After DMSO treatment , bak , bcl w , bfl 1 , fas , and caspases 1 and 9 were up regulated , whereas bik , bcl 2 , and caspases 2 , 3 , and 10 were down regulated at different degrees , achieving mRNA expression levels that correlated with those detected in peripheral blood neutrophils . ^^^ Peripheral blood neutrophils expressed bak , bad , bcl w , bfl 1 , fas , and caspases 1 , 3 , 4 , and 7 10 , but hardly expressed bcl 2 , bcl xL , bik , bax , and caspase 2 . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| The identification of the BH 3 domain as a potent mediator of cell death has led to the emergence of an additional family of proapoptotic proteins ( such as Bad , Bik , Bid and Hrk ) that share identity with Bcl 2 only within this death domain . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| In contrast , certain pro apoptotic proteins such as BIK and BID share a single death effector domain , BH 3 , with other BCL 2 family proteins . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Here , we found that Bik mutants , in which threonine 33 and / or serine 35 were changed to aspartic acid to mimic the phosphorylation at these two residues , enhanced their binding affinity with the antiapoptotic proteins Bcl 10 ( L ) and Bcl 2 and were more potent than wild type Bik in inducing apoptosis and inhibiting cell proliferation in various human cancer cells . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Proapoptotic BH 3 only Bcl 2 family member Bik / Blk / Nbk is expressed in hemopoietic and endothelial cells but is redundant for their programmed death . ^^^ Moreover , whereas loss of the BH 3 only protein Bim rescued mice lacking the prosurvival protein Bcl 2 from fatal polycystic kidney disease and lymphopenia , loss of Bik did not . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| We demonstrate constitutive expression of BAX in virtually all embryos at all stages of development , and variable expression of BCL 2 , BCL XL , BCL W , MCL 1 BAK , BAD , BOKL , BID , BIK , and BCL XS . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of their substrates indicate that they regulate the turnover of key cell cycle proteins ( p27Kip1 , p21Cip1 , p57Kip2 , cyclin E ) , tumor suppressor proteins ( p 53 , RB ) , signaling kinases ( Src , Zap 70 , PI 3 kinase ) , apoptosis regulators ( Bcl 2 , Bax , Bik ) and transcription factors ( Myc , NF kappaB , E1F1 ) , all of which have been implicated in the pathogenesis of malignant lymphoma . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| In accord with their noncycling , antiapoptotic status in vivo , CLL cells displayed high constitutive expression of Bcl 2 and Flip mRNA , while Survivin , Bid and Bik were absent . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Specifically , we observe that Abeta significantly reduces expression of antiapoptotic Bcl w and Bcl 10 ( L ) , mildly affects expression of bim , Bcl 2 , and bax , but does not alter expression of bak , bad , bik , bid , or BNIP3 . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| To examine p 75 ( NTR ) dependent apoptosis in tumor cells , we demonstrated that a dose dependent increase in p 75 ( NTR ) expression was associated with a concomitant increase in the mitochondrial proapoptotic effector proteins Bad , Bax and Bik and a decrease in the mitochondrial prosurvival effector proteins phospho Bad , Bcl 2 and Bcl 10 ( L ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| BIK , a BH ( Bcl 2 homology domain ) 3 only protein , is a proapoptotic member of the BCL 2 family . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| In addition , in contrast to the anti apoptotic Bcl 2 proteins , BHRF 1 does not bind tightly to peptides derived from the pro apoptotic proteins Bak , Bax , Bik , and Bad . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that the adenovirus E4orf6 protein inhibits apoptosis mediated by BNIP 3 and Bik , which are BH 3 only proteins of the Bcl 2 family . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Subsequent studies showed that combined treatment with bortezomib or MG 132 resulted in an increase of death receptor ( DR ) 5 and Bik at protein levels but had no effects on protein levels of DR 4 , Bax , Bak , Bcl 2 , Bcl XL or Flice inhibitory protein ( FLIP ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| BH 3 only members of the Bcl 2 intracellular protein family , which include Bim , Bmf , Bik , Bad , Bid , Puma , Noxa and Hrk , mediate many developmentally programmed and induced cytotoxic signals . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Concomitant loss of proapoptotic BH 3 only Bcl 2 antagonists Bik and Bim arrests spermatogenesis . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| PS 341 activated the BH 3 only proteins Bik and Bim and down regulated Bcl 2 and Bcl xL mRNA and protein expression . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Both Bcl 2 and E1B 19K interacted with Nbk in vitro , and this interaction was highly specific . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Induction of cell death by the BH 3 only Bcl 2 homolog Nbk / Bik is mediated by an entirely Bax dependent mitochondrial pathway . ^^^ Nbk / Bik ( natural born killer / Bcl 2 interacting killer ) is a tissue specific BH 3 only protein whose molecular function is still largely unknown . ^^^ Immunoprecipitation studies revealed that Nbk interacts with Bcl 10 ( L ) and Bcl 2 but not with Bax . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Degradation of the proapoptotic proteins Bik , Puma , and Bim with Bcl 2 domain 3 homology in Chlamydia trachomatis infected cells . ^^^ We now report that C . trachomatis infection leads to degradation of Bik , Puma , and Bim , three upstream proapoptotic BH 3 only proteins of the Bcl 2 family that can transmit death signals to mitochondria by inhibiting the Bcl 2 antiapoptotic proteins and / or activating the Bcl 2 proapoptotic members , such as Bax and Bak . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| Indeed , bortezomib induced increases of Bik and / or Bim in multiple cell lines but not notably of two other BH 3 only proteins ( Puma and Bid ) nor other family members ( Bax , Bak , Bcl 2 , and Bcl xL ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| BIK , a pro apoptotic BH 3 only member of the BCL 2 family , targets the membrane of the endoplasmic reticulum ( ER ) . ^^^ |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13323 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|