| Interacting proteins: Q9UM11 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Control of the SCF ( Skp 2 Cks1 ) ubiquitin ligase by the APC / C ( Cdh 1 ) ubiquitin ligase . ^^^ Here we show that both Skp 2 and Cks 1 proteins are unstable in G 1 and that their degradation is mediated by the ubiquitin ligase APC / C ( Cdh 1 ) ( anaphase promoting complex / cyclosome and its activator Cdh 1 ) . ^^^ Silencing of Cdh 1 by RNA interference in G 1 cells stabilizes Skp 2 and Cks 1 , with a consequent increase in p 21 and p 27 proteolysis . ^^^ Depletion of Cdh 1 also increases the percentage of cells in S phase , whereas concomitant downregulation of Skp 2 reverses this effect , showing that Skp 2 is an essential target of APC / C ( Cdh 1 ) . ^^^ Expression of a stable Skp 2 mutant that can not bind APC / C ( Cdh 1 ) induces premature entry into S phase . ^^^ |
|
| Interacting proteins: Q9UM11 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Here , we show that the F box protein Skp 2 is polyubiquitinated , and hence earmarked for destruction , by APC ( CDH 1 ) . ^^^ As a result , accumulation of SCF ( SKP 2 ) requires prior inactivation of APC ( CDH 1 ) . ^^^ |
|
| Interacting proteins: Q9UM11 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Crashing waves of destruction : the cell cycle and APC ( Cdh 1 ) regulation of SCF ( Skp 2 ) . ^^^ |
|
| Interacting proteins: Q9UM11 and Q13309 |
Pubmed |
SVM Score :0.0 |
| By keeping the levels of Skp 2 and Cks 1 low during G ( 1 ) progression , APC / C ( Cdh 1 ) prevents unscheduled degradation of SCF ( Skp 2 ) substrates and premature entry into S phase . ^^^ Thus , APC / C ( Cdh 1 ) , a ubiquitin ligase involved in mitotic exit and maintenance of G ( 0 ) / G ( 1 ) phase , directly controls SCF ( Skp 2 ) , a ubiquitin ligase involved in the regulation of S phase entry . . ^^^ |
|
| Interacting proteins: Q9UM11 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Two new studies now suggest that SKP 2 accumulation is determined by a second ubiquitin ligase , the anaphase promoting complex or cyclosome , APC / C ( CDH 1 ) . ^^^ |
|