| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :1.1159481 |
| We showed that the F box protein Skp 2 specifically interacted with human Cdt 1 in a phosphorylation dependent manner . 1.1159481^^^ |
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| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :0.72950365 |
| Overexpression of cyclin dependent kinase inhibitors such as p 21 and p 27 dramatically suppresses the phosphorylation of Cdt 1 , disrupts the interaction of Cdt 1 with the F box protein Skp 2 , and blocks the degradation of Cdt 1 . 0.72950365^^^ |
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| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :0.66479944 |
| Using phosphopeptide mapping and mutagenesis studies , we found that threonine 29 within the N terminus of Cdt 1 is phosphorylated by Cdk 2 and required for interaction with Skp 2 . 0.66479944^^^ |
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| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Cdk phosphorylation resulted in the binding of Cdt 1 to the F box protein Skp 2 and subsequent degradation . ^^^ |
|
| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :0.0 |
| In the case of UV treatment , phosphorylation of Cdt 1 induced the recruitment of Cdt 1 to a SCF ( Skp 2 ) complex . ^^^ |
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| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :0.0 |
| We suggest that the Cul 4 Ddb1 ligase evolved to ubiquitinate Cdt 1 during normal cell growth as well as in response to DNA damage and a separate E 3 ligase , possibly SCF ( Skp 2 ) , evolved to either share or take over the function of Cdt 1 ubiquitination during normal cell growth and that PCNA is involved in mediating Cdt 1 degradation by the Cul 4 Ddb1 ligase in response to DNA damage . . ^^^ |
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| Interacting proteins: Q9H211 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Two E 3 ubiquitin ligases , SCF Skp 2 and DDB 1 Cul4 , target human Cdt 1 for proteolysis . ^^^ Consistently , in HeLa cells cosilenced of Skp 2 and Cul 4 , Cdt 1 remained stable in S G 2 phases . ^^^ |
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