| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| The F box protein Skp 2 is a ubiquitylation target of a Cul 1 based core ubiquitin ligase complex : evidence for a role of Cul 1 in the suppression of Skp 2 expression in quiescent fibroblasts . ^^^ The ubiquitin protein ligase SCF ( Skp 2 ) is composed of Skp 1 , Cul 1 , Roc1 / Rbx1 and the F box protein Skp 2 , the substrate recognition subunit . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| In contrast to substrates of the SKP 1 Cullin 1 F box ( SCF ) complexes , in vitro ubiquitination of E2F1 by CUL 1 ROC1 ligase does not require E2F1 phosphorylation , is not stimulated by overexpression of F box protein SKP 2 , and is not affected by immunodepletion of SKP 1 or mutations in CUL 1 disrupting SKPI binding . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| We found the majority of CUL 1 is in a complex with CAND 1 and ROC 1 independent of SKP 1 and F box protein SKP 2 . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| According to a current model , the ubiquitination of p 27 during S phase progression is mediated by SCF ( Skp 2 ) E 3 ligase that captures Thr 187 phosphorylated p 27 by means of the F box protein Skp 2 , which in turn couples the bound substrate via Skp 1 to a catalytic core complex composed of Cul 1 and the Rbx / Roc RING finger protein . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Association of human CUL 1 and ubiquitin conjugating enzyme CDC 34 with the F box protein p 45 ( SKP 2 ) : evidence for evolutionary conservation in the subunit composition of the CDC 34 SCF pathway . ^^^ Here we identify human CUL 1 , a member of the cullin family , and the ubiquitin conjugating enzyme CDC 34 as additional partners of p 45 ( SKP 2 ) in vivo . ^^^ CUL 1 also associates with cyclin A and p 19 ( SKP 1 ) in vivo and , with p 45 ( SKP 2 ) , they assemble into a large multiprotein complex . ^^^ The data presented here imply that the p 45 ( SKP 2 ) CUL 1 p 19 ( SKP 1 ) complex may be a human representative of an SCF type E 3 ubiquitin protein ligase . ^^^ Finally , we show that multiprotein complex formation involving p 45 ( SKP 2 ) CUL 1 and p 19 ( SKP 1 ) is governed , in part , by periodic , S phase specific accumulation of the p 45 ( SKP 2 ) subunit and by the p 45 ( SKP 2 ) bound cyclin A CDK 2 . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Human CUL 1 , but not other cullin family members , selectively interacts with SKP 1 to form a complex with SKP 2 and cyclin A . ^^^ This CUL 1 SKP1 interaction is mediated by the NH 2 terminal domains of both proteins , and the association appears to be required for the interaction of CUL 1 with SKP 2 , an essential element of the S phase cyclin A CDK 2 kinase . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| The identification of CUL 1 as a member of the complex raises the possibility that the p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex may function as the yeast SKP 1 CDC53 F box ( SCF ) protein complex that acts as a ubiquitin E 3 ligase to regulate the G1 / S transition . ^^^ To determine the potential in vivo targets of the p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex , we have used the specific antisense oligodeoxynucleotides against either SKP 1 , SKP 2 , or CUL 1 RNA to inhibit their expression . ^^^ These data suggest that the human p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex is likely to function as an E 3 ligase to selectively target cyclin D and p 21 for the ubiquitin dependent protein degradation . ^^^ Aberrant expression of human p 19 ( SKP 1 ) / p45 ( SKP 2 ) / CUL 1 complex thus may contribute to tumorigenesis by regulating the protein levels of G 1 cell cycle regulators . . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Immunodepletion of components of the complex Cul 1 , Skp 1 , or Skp 2 from the extract abolished p 27 degradation , while addition of purified SCF ( Skp 2 ) to Skp 2 depleted extract restored the capacity to degrade p 27 . ^^^ Skp 2 dependent associations between Skp 1 or Cul 1 and the p 27 phosphopeptide were also detected . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Modification of cullin 1 by ubiquitin like protein Nedd 8 enhances the activity of SCF ( skp 2 ) toward p 27 ( kip 1 ) . ^^^ When the effect of the Nedd 8 modification on the SCF ( skp 2 ) activity toward p 27 ( kip 1 ) was investigated , the activity was markedly decreased by using the Nedd 8 unmodified mutant cullin 1 ( K696R ) , indicating that the modification may play an important role on the SCF ( skp 2 ) activity toward p 27 ( kip 1 ) . . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Here , we provide several lines of evidence that c Myc promotes ubiquitin dependent proteolysis by directly activating expression of the Cul 1 gene , encoding a critical component of the ubiquitin ligase SCF ( SKP 2 ) . ^^^ The cell cycle inhibitor p 27 ( kip 1 ) is a known target of the SCF ( SKP 2 ) complex , and Myc induced Cul 1 expression matched well with the kinetics of declining p 27 ( kip 1 ) protein . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| We have reported previously that the degradation of p 27 requires its phosphorylation on Thr 187 and is mediated by Skp 2 , an F box protein that associates with Skp 1 , Cul 1 , and Roc1 / Rbx1 to form the SCF ( Skp 2 ) ubiquitin ligase complex . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| We found that the mechanism of ATRA induced ubiquitylation of cyclin D 1 and Skp 2 is independent of CUL 1 expression and that ATRA can rescue cyclin D 1 degradation in the uterine cell line SK UT 1 , where D type cyclins are stabilized due to a specific defect in proteolysis . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| As for this mechanism , GATA 2 was found to inhibit ubiquitin / proteasome dependent degradation of p 21 ( WAF 1 ) and p 27 ( Kip 1 ) and to induce their accumulation by repressing the expression of Skp 2 and Cul 1 , both of which are components of the ubiquitin ligase for p 21 ( WAF 1 ) and p 27 ( Kip 1 ) . ^^^ Overexpression of c myc restored the expression of Skp 2 and Cul 1 mRNA , reduced the amounts of p 21 ( WAF 1 ) and p 27 ( Kip 1 ) proteins , and canceled GATA 2 induced growth suppression , suggesting that down regulation of c myc expression may be primarily responsible for GATA 2 induced growth suppression . ^^^ GATA 2 / ER suppressed cytokine dependent growth of MNCs and Sca 1 ( + ) Lin ( ) cells by about 70 % , which was also accompanied by the reduced expression of c myc , Skp 2 , and Cul 1 mRNA and the accumulation of p 21 ( WAF 1 ) and p 27 ( Kip 1 ) proteins . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting and in vitro ubiquitination assays indicated that the expression of Cul 1 and Skp 2 and ubiquitination activity toward p 27 ( Kip 1 ) were not regulated by NSAIDs . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| We found that p 130 interacts with SKP 1 , Cul 1 and SKP 2 in human 293 cells . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Antisense Skp 2 oligonucleotides and a dominant interfering Cul 1 ( 1 452 ) mutant prevented down regulation of p27Kip1S10A , whereas Skp 2 overexpression elicited its destruction in mitogen deprived cells . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we show that E 7 interacts with the SCF ( Skp Cullin F box ) ubiquitin ligase complex containing Cullin 1 ( Cul 1 ) and Skp 2 and can be ubiquitinated by the Cul 1 containing ubiquitin ligase in vitro . ^^^ Coimmunoprecipitation analyses revealed that E 7 interacts with Skp 2 and Cul 1 in vivo . ^^^ Taken together , these results suggest that the Cul 1 and Skp 2 containing ubiquitin ligase plays a role in the ubiquitination and proteolysis of E 7 . ^^^ The papillomavirus E 7 oncoprotein is ubiquitinated by UbcH 7 and Cullin 1 and Skp 2 containing E 3 ligase . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Unlike differentiating J2E cells , Mlf 1 expressing cells did not downregulate Cul 1 and Skp 2 , components of the ubiquitin E 3 ligase complex SCF ( Skp 2 ) involved in the proteasomal degradation of p 27 ( Kip 1 ) . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| ARF expression also led to decreased levels of Cul 1 and Skp 2 , two proteins involved in p 27 degradation . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| The SCF ( Skp 2 ) complex ( consisting of Rbx 1 , Cul 1 , Skp 1 , and Skp 2 ) is one of the E 3 ubiquitin ligases involved in ubiquitination of p 21 ( Cip1 / WAF1 ) . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| We examined this problem for the case of SCF ( Skp 2 ) , a cullin 1 ( Cul 1 ) containing ubiquitin ligase complex that contains the S phase associated protein Skp 2 as the substrate binding F box protein subunit . ^^^ Because levels of Skp 2 , cyclin E , and the accessory protein Cks 1 ( cyclin kinase subunit 1 ) all rise at the end of G ( 1 ) phase , it seemed possible that the neddylation of Cul 1 in SCF ( Skp 2 ) is regulated by the availability of the F box protein and / or the substrate . ^^^ We found that the supplementation of Skp 2 Skp1 and substrate ( along with further components necessary for substrate presentation to the ubiquitin ligase ) to extracts of HeLa cells synergistically increased levels of neddylated Cul 1 . ^^^ Skp 2 Skp1 abrogates the inhibitory influence of CAND 1 on the neddylation of Cul 1 by promoting the dissociation of the cullin CAND 1 complex , whereas substrate , together with substrate presenting components , prevents the action of CSN to deneddylate cullin . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| Ubiquitination of p 27 ( kip 1 ) is performed by the SCF ( skp 2 ) ubiquitin ligase comprised of the core components Roc 1 , Cul 1 and Skp 1 and the substrate recognition components Skp 2 and Cks 1 . ^^^ Here we show that in primary human T lymphocytes , the SCF ( skp 2 ) core components Roc 1 , Cul 1 and Skp 1 are constitutively expressed , and their levels remain unchanged upon TCR / CD3 plus CD 28 costimulation . ^^^ |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13309 and Q13616 |
Pubmed |
SVM Score :0.0 |
| NA |
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