| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| In normal and transformed cells , the F box protein p 45 ( SKP 2 ) is required for S phase and forms stable complexes with p 19 ( SKP 1 ) and cyclin A cyclin dependent kinase ( CDK ) 2 . ^^^ CUL 1 also associates with cyclin A and p 19 ( SKP 1 ) in vivo and , with p 45 ( SKP 2 ) , they assemble into a large multiprotein complex . ^^^ Finally , we show that multiprotein complex formation involving p 45 ( SKP 2 ) CUL 1 and p 19 ( SKP 1 ) is governed , in part , by periodic , S phase specific accumulation of the p 45 ( SKP 2 ) subunit and by the p 45 ( SKP 2 ) bound cyclin A CDK 2 . ^^^ The dependency of p 45 ( SKP 2 ) p 19 ( SKP 1 ) complex formation on cyclin A CDK 2 may ensure tight coordination of the activities of the cell cycle clock with those of a potential ubiquitin conjugation pathway . . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Overexpression of cyclin A but not Skp 2 correlates with the tumor relapse of human hepatocellular carcinoma . ^^^ Increased levels of Skp 2 , a cyclin A interacting protein , were also found in 17 of 31 ( 55 % ) of HCC patients who showed a trend to have more S phase tumor cells ( P = 0 . 07 ) . ^^^ By an unpaired Student ' s t test and a Fisher ' s exact or chi 2 analysis , overexpression of cyclin A had a strong correlation with elevated Skp 2 expression and increased alpha fetoprotein levels ( P = 0 . 001 and 0 . 009 , respectively ) , but it was not associated with patients ' age , tumor size , cirrhosis , or the positive detection of hepatitis B virus surface antigen . ^^^ By multivariate analysis , the correlation of cyclin A overexpression with shorter disease free periods remained significant after adjustment for Skp 2 overexpression and alpha fetoprotein induction ( P = 0 . 019 ) . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Human CUL 1 , but not other cullin family members , selectively interacts with SKP 1 to form a complex with SKP 2 and cyclin A . ^^^ This CUL 1 SKP1 interaction is mediated by the NH 2 terminal domains of both proteins , and the association appears to be required for the interaction of CUL 1 with SKP 2 , an essential element of the S phase cyclin A CDK 2 kinase . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Inhibition of DNA synthesis by downregulation of cyclin A but not Skp 2 overexpression in human hepatocellular carcinoma cells . ^^^ Cyclin A is an S and G2 / M phase regulatory protein and associates with Skp 2 in many transformed cells . ^^^ Our previous results showed that 12 ( 39 % ) and 17 ( 55 % ) out of 31 hepatocellular carcinoma ( HCC ) patients exhibited higher protein expression levels of cyclin A and Skp 2 , respectively , in their tumorous compared to non tumorous tissues . ^^^ In the present study , we used Western blot analysis to show that 3 out of 6 HCC cell lines , HA59T , HA22T and HCC 36 , exhibited overexpression of cyclin A , among which the HCC 36 cell line also expressed a higher Skp 2 protein level . ^^^ Moreover , we used the antisense oligonucleotide phosphorothioates to down regulate the overexpression of cyclin A and Skp 2 proteins to determine whether or not these two proteins are involved in the mitogenesis of HCC 36 cells . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Later , as cyclin A accumulates and cells enter S phase , hOrc1p is ubiquitinated on chromatin and then degraded . hOrc1p destruction occurs through the proteasome and is signaled in part by the SCF ( Skp 2 ) ubiquitin ligase complex . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Moreover , the percentages of Skp 2 and S phase positive cells , as measured by DNA content or BrdU labeling , strictly matched and closely parallel that of Ki 67 and cyclin A . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Finally , increasing the amount of both cyclin E cdk 2 and skp 2 was less efficient at promoting p 27 ubiquitination than was increasing the amount of cyclin A cdk 2 alone in extracts prepared from cultures of > 93 % purified G ( 1 ) cells . ^^^ Together these lines of evidence suggest that cyclin A cdk 2 plays an ancillary noncatalytic role in the ubiquitination of p 27 by the SCF ( skp 2 ) complex . . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| To answer these questions in colorectal tumors , Skp 2 , cyclin A , cyclin B 1 , cyclin E , CDK 2 , and Ki 67 were immunohistochemically stained in 12 normal mucosa , 36 adenomas , 11 carcinomas in adenomas , 102 primary carcinomas , and 12 paired lymph node metastases ; and Skp 2 was examined by Western blot in 8 pairs of normal mucosa and carcinomas . ^^^ High Skp 2 was also significantly linked with elevated cyclin A , cyclin B 1 , cyclin E , CDK 2 ( in primary carcinomas only ) , and Ki 67 in both adenomas and primary carcinomas . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| The LI of skp 2 in endometrial carcinoma was significantly correlated with that of p 27 , Ki 67 , cdk 2 , cyclin A , cyclin D 1 , cyclin E , p 53 and PTEN . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : Clinicopathologic features and tissue microarray based immunohistochemical expression of p 27 ( Kip 1 ) , Skp 2 , Cks 1 , cyclin E , cyclin A , Ki 67 , and minichromosome maintenance protein 2 ( Mcm 2 ) were assessed in 70 primary myxofibrosarcomas and correlated with clinical outcomes . ^^^ RESULTS : High indices of Skp 2 ( > or =10 % ) , cyclin A ( > or =10 % ) , and Mcm 2 ( > or =50 % ) were adverse prognosticators at the univariate level . ^^^ Furthermore , co overexpression of Skp 2 and cyclin A identified highly lethal cases in the entire cohort [ P < 0 . 0001 for disease specific survival ( DSS ) , P = 0 . 0004 for overall survival ( OS ) ] and the lower grade subset ( Fdration Nationale des Centres de Lutte Contre le Cancer grade 1 and 2 ; P = 0 . 0006 for DSS , P = 0 . 0093 for OS ) . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| In response to tax , cyclin A , cyclin B 1 , securin , and Skp 2 becomes polyubiquitinated and degraded starting in S phase . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Skp 2 contains a novel cyclin A binding domain that directly protects cyclin A from inhibition by p27Kip1 . ^^^ Skp 2 also forms complexes with cyclin A , which is particularly abundant in cancer cells due to frequent Skp 2 overexpression , but the mechanism and significance of this interaction remain unknown . ^^^ Here , we report that Skp 2 cyclin A interaction is mediated by novel interaction sequences on both Skp 2 and cyclin A , distinguishing it from the well known RXL hydrophobic patch interaction between cyclins and cyclin binding proteins . ^^^ Furthermore , a short peptide derived from the mapped cyclin A binding sequences of Skp 2 can block Skp 2 cyclin A interaction but not p 27 cyclin A interaction , whereas a previously identified RXL peptide can block p 27 cyclin A interaction but not Skp 2 cyclin A interaction . ^^^ Functionally , Skp 2 cyclin A interaction is separable from Skp 2 ability to mediate p 27 ubiquitylation . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| Oncostatin M induces growth arrest by inhibition of Skp 2 , Cks 1 , and cyclin A expression and induced p 21 expression . ^^^ |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P20248 and Q13309 |
Pubmed |
SVM Score :0.0 |
| NA |
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