| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| To examine whether semaphorins could modulate VEGF endothelial cell guidance cues in the developing kidney , we studied the expression of semaphorin 3A and semaphorin 3F and their receptors NP 1 and NP 2 in the kidney during ontogeny using Northern blot analysis , in situ hybridization , Western blot analysis and immunohistochemistry . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| We have found that semaphorin 3F ( s3f ) , a semaphorin which signals through the np 2 receptor , was able to inhibit VEGF ( 165 ) , as well as bFGF induced proliferation of HUVECs . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| During lung tumorigenesis , both NRP and VEGF expression increase on dysplastic lung epithelial cells ; SEMA3F expression is reduced and SEMA3F protein is delocalized from the membrane to the cytoplasm . ^^^ Conversely , more aggressive tumors are associated with increased VEGF staining and a corresponding loss in membranous SEMA3F . . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| Semaphorin SEMA3F and VEGF have opposing effects on cell attachment and spreading . ^^^ In primary tumors , SEMA3F and VEGF surface staining is inversely correlated . ^^^ These results support the concept that SEMA3F and VEGF have antagonistic actions affecting motility in primary tumor cell . . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| Expression of VEGF , semaphorin SEMA3F , and their common receptors neuropilins NP 1 and NP 2 in preinvasive bronchial lesions , lung tumours , and cell lines . ^^^ Elevated VEGF levels are associated with the loss and cytoplasmic delocalization of SEMA3F in lung cancer , suggesting competition for their NP 1 and NP 2 receptors . ^^^ To determine the timing of these events , we compared by immunohistochemistry VEGF , SEMA3F , NP 1 and NP 2 expression in 50 preneoplastic lesions and 112 lung tumours . ^^^ In preneoplastic lesions , VEGF increased from low grade to high grade dysplasia ( p=0 . 001 ) whereas SEMA3F levels remained low . ^^^ Migrating cells overexpressed VEGF , SEMA3F , NP 1 and NP 2 with cytoplasmic delocalization of NP 1 as demonstrated in an in vitro wound assay . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| Induction of neuropilins 1 and 2 and their ligands , Sema3A , Sema3F , and VEGF , during Wallerian degeneration in the peripheral nervous system . ^^^ Neurol . 172 ( 2001 ) 398 ) that VEGF mRNA levels rise during Wallerian degeneration in the PNS and herein demonstrate that NP 1 , NP 2 , Sema3A , and Sema3F mRNA levels increase in peripheral nerves distal to a transection or crush injury . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| In lung cancer specimens , antibody staining against SEMA3F has been shown to correlate with stage and histologic subtypes with more aggressive tumors showing increased VEGF and decreased SEMA3F staining . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| Previously , we demonstrated that loss of SEMA3F , a secreted semaphorin encoded in 3p21 . 3 , is associated with higher stages in lung cancer and primary tumor cells studied with anti vascular endothelial growth factor ( VEGF ) and SEMA3F antibodies . ^^^ In vitro , SEMA3F inhibits cell spreading ; this activity is opposed by VEGF . ^^^ These results suggest that VEGF and SEMA3F compete for binding to their common neuropilin receptor . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| Although both antagonize VEGF binding / response to neuropilin ( NRP ) receptors , in lung cancer lines , SEMA3F is predominantly expressed and preferentially utilizes NRP 2 . ^^^ In lung cancer patients , SEMA3F loss correlates with advanced disease and increased VEGF binding to tumor cells . ^^^ In cell lines , VEGF enhances adhesion and migration in an integrin dependent manner , and exogenous SEMA3F causes cells to round and lose extracellular contacts . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| In contrast , SEMA3F , another ligand for NRP 2 , was able to inhibit human endothelial cell survival and migration induced by VEGF A and VEGF C . ^^^ |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13275 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
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