Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Because N methyl D aspartate ( NMDA ) glutamate ionotropic receptors are involved in the occurrence of audiogenic seizures , we investigated by in situ hybridization methods , the expression of the different subunits ( NR 1 , NR2A , NR2B , NR2C ) of this receptor in the central nucleus of the inferior colliculus ( IC ) , a main relay of the auditory pathways . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Application of 500 microM picrotoxin for two days led to decreases in the messenger RNA levels for the N methyl D aspartate receptor subunits , NR2A and NR2B , and for the non N methyl D aspartate receptor subunits , glutamate receptors 1 and glutamate receptors 2 , to about 50 % of the levels seen in control cultures . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We used in situ hybridization to examine the effect of removal of the glutamatergic input to the striatum by unilateral frontal cortical ablation on the expression of genes encoding subunits from three families of glutamate receptors : N methyl D aspartate receptors ( NMDAR 1 , NMDAR2A , and NMDAR2B ) ; alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) receptors ( GluR 1 4 , flip and flop splice variants ) ; and metabotropic receptors ( mGluR 1 5 ) . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Stimulation by histamine was dependent on the concentration of agonist used to activate NR1A / NR2B receptors and was seen with high but not low concentrations of NMDA and glutamate . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In the present work we report the localization of the gene encoding the human NMDAR2B receptor subunit ( called GRIN2B for glutamate receptor , ionotropic , N methyl D aspartate 2B ) to chromosome 12p12 by in situ hybridization and somatic cell hybrids . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Transcript levels of the NMDA receptor genes NMDAR 1 , NR2A , NR2B , NR2C , and NR2D and a glutamate binding protein ( GBP ) were not altered by kindling . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
At NR1A / NR2B receptors , in the presence of a saturating concentration of glycine , the magnitude of spermine stimulation was dependent on the concentration of NMDA or glutamate . ^^^ Spermine induced a small ( 1 . 2 1 . 5 fold ) decrease in the affinity of NR1A / NR2B receptors for NMDA and glutamate . ^^^ Endogenous polyamines could act as a bidirectional gain control at some native NMDA receptors containing the NR1A and NR2B subunits , by dampening the response to low concentrations of glutamate and enhancing the response to high concentrations of glutamate . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Glutamate was a full agonist at both receptors , having a modestly but statistically significant ( p < 0 . 002 ) higher affinity for the NR2B than the NR2A containing receptor ( microscopic Kd [ mKd ] = 0 . 76 and 0 . 43 microM , respectively ) . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We further characterized the glutamate site assembled from NR1a , NR2A , and NR2B NMDA receptor subunits using L [ 3H ] glutamate and [ 3H ] CGP 39653 binding assays . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Changes of NMDA receptor subunit ( NR 1 , NR2B ) and glutamate transporter ( GLT 1 ) mRNA expression in Huntington ' s disease an in situ hybridization study . ^^^ The distribution of NMDA receptor subunit ( NR 1 , NR2B ) and glia bound glutamate transporter ( GLT 1 ) mRNAs was investigated in postmortem brains of Huntington ' s disease ( HD ) patients and controls by means of in situ hybridization using radiolabeled deoxyoligonucleotides . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Molecular determinants of agonist discrimination by NMDA receptor subunits : analysis of the glutamate binding site on the NR2B subunit . ^^^ Here , we demonstrate that the glutamate binding site is located on the homologous regions of the NR2B subunit . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
With the advent of powerful new microchemical tools and molecular genetic methods , three new classes of proteins have been identified in the PSD at glutamatergic synapses : the PSD 95 family , the NR2B subunit of the NMDA type glutamate receptor , and densin 180 . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We have investigated the developmental change in NMDA receptor affinity for both glutamate and glycine in acutely dissociated parietal cortex neurons of the rat , together with the expression during ontogeny of NR2A and NR2B mRNA and protein . ^^^ Whereas there is little change in NMDA receptor glutamate affinity with age , a population of NMDA receptors emerges in 14 and 28 d old animals with a markedly reduced affinity for glycine ( mKD = approximately 800 nM ) and a reduced sensitivity to the NR2B subunit selective NMDA antagonist ifenprodil . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Here , the numbers of glycine binding NR 1 and glutamate binding NR 2 subunits in the NMDA receptor hetero oligomer were determined by coexpressing the wild type ( wt ) NR 1 with the low affinity mutant NR 1 ( Q387K ) , and the wt NR2B with the low affinity mutant NR2BE387A , subunits in Xenopus oocytes . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Specific binding , defined with a new NR2B specific antagonist , Ro 04 5595 [ 1 ( 4 chlorophenyl ) 2 methyl 6 methoxy 7 hydroxy 1 , 2 , 3 , 4 tetrahydroisoqu inoline ] , at 10 microM , was fully inhibited by several compounds with the following rank order of affinities Ro 25 6981 > CP 101 , 606 > Ro 04 5595 = ifenprodil > > eliprodil > haloperidol > spermine > spermidine > MgCl 2 > CaCl 2 and partially inhibited by competitive glutamate recognition site antagonists . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Mutation of D 669 to asparagine ( D669N ) , alanine ( D669A ) , or glutamate ( D669E ) abolished the `` glycine independent ' ' form of spermine stimulation in heteromeric NR1A / NR2B receptors . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The rank order of potency was quinolinic acid < NMDA < homoquinolinic acid < or = glutamate for the NR 1 + NR2A and NR 1 + NR2B combinations whereas for NR 1 + NR2C it was quinolinic acid < < < < homoquinolinic acid < NMDA < or = glutamate . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The effect of treatment with the D 1 dopamine receptor agonist SKF 38393 on the expression of metabotropic glutamate receptor 1 , 3 , 4 and 5 receptor subtypes and of the glutamate N methyl D aspartate ionotropic receptor subunits NRI , NR2A and NR2B was analysed using in situ hybridization . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Here , we investigated the co expression of the neurotrophin 4 / brain derived neurotrophic factor receptor TrkB with the N methyl D aspartate glutamate receptor subunits NR 1 C1 , NR2A and NR2B , on postnatal days 10 and 22 and in the adult rat primary visual cortex . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
To investigate the role of NMDA receptor subunits in the neuroendocrine functions of the magnocellular neuroendocrine cells of the hypothalamus , we examined the effects of osmotic stimulation on the protein expression of the NMDA receptor subunits , NR 1 and NR2B , important in binding glycine and glutamate , respectively . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Quantitative in situ hybridization revealed that following the induction of hippocampal long term potentiation ( LTP ) in the dentate gyrus of freely moving rats , specific increases in the expression of the NR2B subunit of the N methyl D aspartate ( NMDA ) receptor and mGluR1c , a short splice variant of the metabotropic glutamate receptors that are linked intracellularly to phospholipase C ( PLC ) and protein kinase C ( PKC ) , were seen in the postsynaptic dentate granule cells . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We measured the glutamate induced increase of calcium concentration in fibroblasts expressing one of the NR 1 splice forms and the NR2B subunit . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Previous investigations utilizing 125I MK 801 binding assays with recombinant receptors distinguished certain structural determinants on the NR2B subunit for these two drugs , with glutamate 201 being critical for haloperidol sensitivity and arginine 337 being important for ifenprodil block . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Differential effects of electroconvulsive shock on the glutamate receptor mRNAs for NR2A , NR2B and mGluR5b . ^^^ In the dentate gyrus , such treatment elevated the mRNAs for the NMDA subunits NR2A and NR2B , but it reduced the mRNA for the metabotropic glutamate receptor mGlu5b . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The main glutamate receptor subunits detected in IS cells were GluR 1 flop and GluR 2 flop , GluR 5 and GluR 6 , and NR2B and NR2D for the alpha amino 3 hydroxyl 5 methyl 4 isoxazolepropionic acid ( AMPA ) , kainate and N methyl D aspartic acid ( NMDA ) subtypes , respectively . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
This study examined the effects on rat behaviour of antagonists acting at various sites on the N methyl D aspartate ( NMDA ) receptor complex , i . e . the glutamate recognition site ( CPP ) , ion channel ( dizocilpine ) , glycine recognition site [ ( + ) HA 966 ] and the NR2B subunit selective compound ifenprodil . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
L glutamate , NMDAR2B and the enzyme glutamine synthetase were identified by using monoclonal antibodies . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Alpha actinin ( alpha actinin 2 ) is a protein which links the NR 1 and NR2B subunits of N methyl D aspartate ( NMDA ) glutamate receptors to the actin cytoskeleton . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Allosteric interactions with polyamines , Mg ( 2+ ) , Zn ( 2+ ) , glutamate , glycine , and their antagonists were consistent with NMDA receptors with NR2B subtype pharmacology . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
At immature synapses , a high probability of glutamate release ( Pr ) was correlated with the expression of postsynaptic NMDA ( N methyl D aspartate ) receptors containing the NR2B subunit . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Here we show that regulated CaMKII interaction with two sites on the NMDA receptor subunit NR2B provides a mechanism for the glutamate induced translocation of the kinase to the synapse in hippocampal neurons . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The time course of the increase in NR2B mRNA most closely followed the increase in glutamate stimulated changes in the magfura 2 signal and neuronal injury . ^^^ Therefore , the increases in the glutamate stimulated [ Ca ( 2+ ) ] ( 1 ) responses and NMDA receptor subunit mRNA levels ( especially NR2B ) are likely involved in the development of susceptibility to excitotoxicity in cultured rat forebrain neurons . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Because native NR2A and NR2B containing receptors are pharmacologically distinct , it should be possible to develop NR2A and NR2B selective glutamate site antagonists . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
This neurotoxic effect could be totally prevented by preincubation with an N methyl D aspartate receptor subunit 2B ( NR2B ) specific antagonist , NR2B antibodies , and an unspecific N methyl D aspartate receptor blocker and was partially blocked with an NR2A specific antagonist but not with NR2A antibodies or alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate receptor and metabotropic glutamate receptor antagonists . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
E2F1 ( + / + ) and ( / ) cultures were comparable , in that they contained similar neuronal densities , responded with similar increases in intracellular calcium concentration ( [ Ca ( 2+ ) ] 1 ) to glutamate receptor agonists , and showed similar NMDA receptor subunit mRNA expression levels for NR 1 , NR2A and NR2B . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NR 1 ( F639A ) did not alter the agonist potency of glutamate but did produce a leftward shift in the glycine concentration response for receptors containing NR2A and NR2B subunits . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
RESULTS : Glutamate receptor expression was lower at both transcriptional ( NMDAR 1 , NMDAR2B , NMDAR2C , gluR 1 , gluR 3 , and KA 2 subunit mRNAs ) and posttranscriptional ( [ ( 3 ) H ] ifenprodil and [ ( 3 ) H ] MDL 105 , 519 binding to polyamine and glycine sites of the NMDA receptor ) levels in the thalamus in patients with schizophrenia than in comparison subjects , but differences were most prominent in nuclei with reciprocal projections to limbic regions . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Furthermore , our biochemical experiments showed that enrichment significantly increased protein levels of GluR 1 , NR2B , and NR2A subunits of glutamate receptors in both wild type and NR2B Tg mice . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
It has also been demonstrated that the level of an ionotropic N methyl d aspartate 2B subunit ( GRIN2B ) of the glutamate receptor tends to increase after subchronic administration of clozapine , suggesting that GRIN2B may play an active role in the pathogenesis of schizophrenia and the function of clozapine medication . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The NR2B subunit of glutamate receptors as a potential target for relieving chronic pain : prospects and concerns . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Lithium protection against glutamate excitotoxicity in rat cerebral cortical neurons : involvement of NMDA receptor inhibition possibly by decreasing NR2B tyrosine phosphorylation . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Whereas PS potentiated NMDA , glutamate , and glycine induced currents of NR1 / NR2A and NR1 / NR2B receptors , it was inhibitory at NR1 / NR2C and NR1 / NR2D receptors . 3 . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Glutamate receptors and persistent pain : targeting forebrain NR2B subunits . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We quantitatively defined the contributions of carboxyl terminal residues to binding selectivity of the prototypic interactions of the PDZ domains of postsynaptic density protein 95 ( PSD 95 ) and its homolog synapse associated protein 90 ( SAP 102 ) with the NR2b subunit of the N methyl d aspartate type glutamate receptor . ^^^ Prominent were a requirement for glutamate or glutamine at P ( 3 ) and for valine at P ( 0 ) for high affinity binding and a preference for threonine over serine at P ( 2 ) , in the context of the last 11 residues of the NR2b COOH terminus . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Computer simulation of the three dimensional structure of the glutamate site of the NR2B subunit of the NMDA receptor . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The immunohistochemical localization of the NMDA glutamate receptor subunits NR 1 , NR2A , and NR2B was investigated in the rat vestibular periphery at the light and electron microscopy level using specific antipeptide antibodies . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The increase in NR2B tyrosine phosphorylation was abolished by intrathecal pretreatment with genistein , a tyrosine kinase inhibitor ; PP 2 , an Src family tyrosine kinase inhibitor ; AIDA , a group 1 metabotropic glutamate receptor antagonist ; L 733 , 060 , an NK 1 tachykinin receptor antagonist , and chelerythrine , a protein kinase C inhibitor . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Here , the effect of Con G on recombinant NMDA receptors carrying point mutations within the glycine and glutamate binding pockets of the NR 1 and NR2B subunits was studied using whole cell voltage clamp recording from cRNA injected Xenopus oocytes . ^^^ Homology based molecular modeling of the glutamate binding region based on the known structure of the glutamate binding site of the AMPA receptor protein GluR 2 suggests how selected amino acid side chains of NR2B might interact with specific residues of Con G . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
These results suggest that big dynorphin induced nociceptive behavior is mediated through the activation of the NMDA receptor ion channel complex by acting on the NR2B subunit and / or the polyamine recognition site but not on the glycine recognition site , and does not involve opioid , non NMDA glutamate receptor mechanisms or tachykinin receptors in the mouse spinal cord . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The glutamate effects were enhanced by glycine application and were prevented by the NMDA receptor blocker MK 801 , as well as by the NR2B selective antagonist ifenprodil . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
By analyzing patch clamp recordings of one channel activated by glutamate , we determined the burst structure and open probability for recombinant rat NR1 / NR2B receptors . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The recombinant AptNR1 / NR2B receptors produced robust currents after stimulation with glutamate or NMDA in the presence of glycine . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Pentamidine inhibited a constitutively active NR 1 ( T648A ) / NR2B NMDA receptor in a manner similar to its inhibition of a glutamate gated wild type NMDA receptor , but different from its inhibition of constitutively active delta 2 ( A654T ) receptors . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The neuroprotective effects of lithium , a mood stabilizer , against glutamate induced excitotoxicity in rat cortical neurons were associated with a decrease in Tyr 1472 phosphorylation of the N methyl D aspartate ( NMDA ) receptor NR2B subunit and a loss of receptor activity . ^^^ A Src kinase inhibitor , SU 6656 , and an NR2B antagonist , ifenprodil , partially blocked glutamate excitotoxicity . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In particular , NMDA receptor antagonists that selectively target the NR2B subunit and antagonists of the metabotropic glutamate receptor mGluR 5 appear to hold promise and deserve future attention . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The activity of the N methyl D aspartate ( NMDA ) receptor , a subclass of ionotropic glutamate receptor , is modulated by a complex network of phosphorylation and dephosphorylation . 1 investigated the relative extent of tyrosine phosphorylation of NMDA receptor subunit 2A ( NR2A ) and 2B ( NR2B ) subunits in the rat forebrain postsynaptic density ( PSD ) fraction . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In cultures treated with vehicle and AraC , glutamate induced neurotoxicity was mediated through stimulation of the NR 1 NR2B subtype of NMDA receptors , because it was blocked by the NMDA receptor antagonist MK 801 and the NR 1 NR2B selective receptor antagonist ifenprodil . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We present new homology based models of the glutamate binding site ( in closed and open forms ) of the NMDA receptor NR2B subunit derived from 10 ray structures of the water soluble AMPA sensitive glutamate receptor . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In this study , the protein expression levels of several ionotropic glutamate receptor subunits ( N methyl D aspartate ( NMDA ) subunits NR 1 , NR2A , NR2B , and alpha amino 3 hydroxy 5 methyl 4 isoxazoleproprionic acid ( AMPA ) receptor subunits GluR 1 , GluR 2 , GluR 4 ) were quantified in particulate preparations from rat spinal cord at various ages after birth . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In addition to changes in NR2B mRNA levels , quantitative polymerase chain reaction analysis revealed that brain derived neurotrophic factor ( BDNF ) and glutamate receptor 5 mRNA levels were also significantly elevated in the DG of Runners , but not in other areas of the hippocampus . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In addition , RT PCR results revealed that glutamate transporters ( GLT 1 and EAAC 1 ) and ionotropic glutamate receptors ( NR 1 , NR2B , GluR 6 and KA 2 ) were expressed in mouse testis . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Association of a glutamate ( NMDA ) subunit receptor gene ( GRIN2B ) with obsessive compulsive disorder : a preliminary study . ^^^ We postulated that N methyl d aspartate ( NMDA ) receptors were involved in OCD , and specifically that polymorphisms in the 3 ' untranslated region of GRIN2B ( glutamate receptor , ionotropic , N methyl d aspartate 2B ) were associated with OCD in affected families . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Chronic PCP treated animals showed significant alterations in glutamate receptor subunits , particularly for the NR 1 , NR2B , NR2C , and NR2D components of the NMDA receptor . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In addition , dorsocaudal brain stem tissue lysates were harvested at 1 h , 6 h , 1 day , 3 days , 7 days , 14 days , and 30 days of SH and IH and analyzed for NR 1 , NR2A , and NR2B NMDA glutamate receptor expression by immunoblotting . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
In studying glutamate receptor regulation of sgk expression , we found that blockade of N methyl d aspartate ( NMDA ) receptors in general , and the NR2B subunit in particular both effectively blocked enrichment induced spatial learning facilitation , but they did not block enrichment induced sgk expression . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Immunofluorescent analysis was used to investigate the existence and location of glutamate , glutamate receptor ( NR2B ) , and glutamate transporter ( GLT 1 ) in mouse and human sperm . ^^^ In addition , reverse transcription polymerase chain reaction technique and sequencing analysis revealed that glutamate transporters ( GLT 1 and EAAC 1 ) and ionotropic glutamate receptors ( NR 1 , NR2B , GluR 6 , and KA 2 ) existed in mouse sperm as well as in human sperm . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Preliminary studies with microdialysis and real time PCR analysis support this idea : local ethanol administration in vivo had no effect on glutamate release , but chronic ethanol nearly tripled the expression of NR2B subunits ( the most ethanol sensitive ) in CeA . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The interaction of calcium / calmodulin dependent protein kinase 2 ( CaMKII ) with the NR2B subunit of N methyl D aspartate type glutamate receptor is thought to be one of the important events leading to synaptic plasticity . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We estimated that up to approximately 30 % of NMDARs contributing to EPSCs were activated by glutamate released from multiple synaptic sources ; however , NMDAR mediated synaptic cross talk was undetectable when NR2B subunit containing receptors were blocked ( but could be rescued by blocking glutamate uptake ) . ^^^ We propose that NR2B containing NMDARs can detect glutamate arising from multiple synapses , whereas NR2A containing NMDARs only normally mediate direct synaptic transmission . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NR2B subunit containing NMDA type glutamate receptors are implicated in the windup of dorsal horn neurons , while their role at the motoneuronal level is controversial . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
However , this decrease was not the same for both subunits , resulting in a decrease ( over 30 % ) in the NR2A : NR2B ratio indicating that the NMDAR had temporarily become more sensitive to glutamate and would remain open longer once activated . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Here , we show that in rats with Freund ' s adjuvant induced inflammation , the increased dorsal horn NR2B tyr P is blocked by group 1 metabotropic glutamate receptor ( mGluR ) antagonists [ 7 ( hydroxyimino ) cyclopropa [ b ] chromen 1a carboxylate ethyl ester ( CPCCOEt ) and 2 methyl 6 ( phenylethynyl ) pyridine ( MPEP ) , by the Src inhibitor CGP 77675 , but not by the MAP kinase inhibitor 2 ' amino 3 ' methoxyflavone . ^^^ Analysis of the calcium pathways shows that the in vivo NR2B tyr P is blocked by an IP 3 receptor antagonist 2 aminoethoxydiphenylborate ( 2APB ) but not by antagonists of ionotropic glutamate receptors and voltage dependent calcium channels , suggesting that the NR2B tyr P is dependent on intracellular calcium release . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
PCR based assays showed that alcoholism was associated with polymorphisms of the dopamine D 2 receptor ( DRD 2 ) TaqI B ( P = . 029 ) and the GABAA beta 2 subunit C1412T ( P = . 012 ) genes , but not with the glutamate receptor subunit gene NMDAR2B ( 366C / G ) , the serotonin transporter gene ( 5HTTL PR ) , the dopamine transporter gene DAT 1 ( SLC6A3 ) , the dopamine D 2 receptor gene DRD 2 TaqI A , or the GABAA alpha 1 ( A15G ) , alpha 6 ( T1519C ) , and gamma 2 ( G3145A ) subunit genes . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Using the crystal structure of the binding region of the ( RS ) 2 amino 3 ( 3 hydroxy 5 methyl 4 isoxazolyl ) propionic acid ( AMPA ) selective GluR 2 subunit , we identified determinants of ligand selectivity and efficacy within the glutamate binding pocket of the NR2B subunit of the N methyl D aspartate ( NMDA ) receptor by site directed mutagenesis . ^^^ Electrophysiological analyses of mutated NR2B polypeptides revealed drastic effects on the affinity of L glutamate but not of the co agonist glycine . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The figure shows the binding mechanism of glutamate with NR2B subunit of the NMDA receptor . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
For example , blockers selective to the NR2B subunit or agents that block metabotropic glutamate receptors or alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid receptors are currently under investigation . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Recordings from outside out patches that contain a single active channel show that NR2A containing receptors have a higher probability of opening at least once in response to a brief synaptic like pulse of glutamate than NR2B containing receptors ( NR2A , 0 . 80 ; NR2B , 0 . 56 ) , a higher peak open probability ( NR2A , 0 . 50 ; NR2B , 0 . 12 ) , and a higher open probability within an activation ( NR2A , 0 . 67 ; NR2B , 0 . 37 ) . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Analysis of glutamate induced apoptosis of the YAC 128 MSN revealed that ( 1 ) actions of glutamate are mediated by mGluR1 / 5 and NR2B glutamate receptors ; ( 2 ) membrane permeable inositol 1 , 4 , 5 trisphosphate receptor blockers 2 APB and Enoxaparin ( Lovenox ) are neuroprotective ; ( 3 ) apoptosis involves the intrinsic pathway mediated by release of mitochondrial cytochrome c and activation of caspases 9 and 3 ; ( 4 ) apoptosis requires mitochondrial Ca2+ overload and can be prevented by the mitochondrial Ca2+ uniporter blocker Ruthenium 360 ; and ( 5 ) apoptosis involves opening of mitochondrial permeability transition pore ( MPTP ) and can be prevented by MPTP blockers such as bongkrekic acid , Nortriptyline , Desipramine , Trifluoperazine , and Maprotiline . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Because a loss of NR2B subunit inclusion in NR receptors would likely confer increased Ca ( ++ ) conductance and faster deactivation kinetics , the stress induced decrease in NR2B mRNA is consistent with enhanced glutamate signaling in the PVN following chronic stress and , perhaps , increased basal HPA activity and more rapid and / or more robust HPA responses to stress . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
To test the predictive value of these models , all four stereoisomers of the antagonist 1 ( phenanthren 2 carbonyl ) piperazine 2 , 3 dicarboxylic acid ( PPDA ) were docked into the NR2B glutamate binding site model . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The increase in glutamate release triggered a long term enhancement of AMPA receptor function that requires activation of postsynaptic NMDA receptors containing NR2B subunits . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The magnitude of reversal was greater than other negative modulators of the NMDA receptor studied , such as uncompetitive channel blockers ( e . g . , memantine , dizocilpine , and ketamine ) , a NR2B selective antagonist ( e . g . , ifenprodil ) , and other glutamate antagonists [ e . g . , selfotel , 3 ( 2 carboxypiperazin 4 yl ) propyl 1 phosphonic acid ( CPP ) , D , L ( E ) 2 amino 4 propyl 5 phosphono 3 pentenoic acid ( CGP 39653 ) ] , up to doses that suppressed operant rates of responding . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Immunoblotting analysis showed that the NR2A and NR2B subunits of NMDARs were markedly degraded in cultured cortical neurons treated with glutamate , and the degradation of NR 2 subunits was prevented by calpain inhibitors . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We show that diminution of network activity in mature neuronal circuit promotes reorganization of neuronal circuits via NR2B subunit containing NMDA type glutamate receptors ( NR2B NMDARs ) , which mediate silent synapse formation . ^^^ The circuit reactivation also caused an increased colocalization of glutamate receptor 1 specfic and synaptic NR2B specific puncta . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The NR2B subunit of N methyl d aspartate ( NMDA ) type glutamate receptor , densin 180 , and alpha actinin bind comparable , approximately stoichiometric amounts of Thr ( 286 ) autophosphorylated CaMKIIalpha , forming a ternary complex ( Robison , A . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Either a nonselective NMDA receptor antagonist ( 5R , 10S ) ( + ) 5 methyl 10 , 11 dihydro 5H dibenzo [ a , d ] cyclohepten 5 , 10 imine hydrogen maleate ( MK 801 ) or selective NR2B subunit containing NMDA receptor antagonists ifenprodil and ( R , S ) alpha ( 4 hydroxyphenyl ) beta methyl 4 ( phenylmethyl ) 1 piperid inepropanol ( Ro 25 6981 ) significantly inhibited the glutamate evoked Ca ( 2+ ) influx into neurons , but not into astrocytes . ^^^ These results suggest that NR2B subunit containing NMDA receptor plays a critical role in not only glutamate evoked Ca ( 2+ ) influx into neurons , but also glutamate induced astrocytic activation . ^^^ Thus , glutamate mediated pathway via NR2B subunit containing NMDA receptor may , at least in part , contribute to neuron to astrocyte signaling . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Traxoprodil ( CP 101 , 606 ) is a novel and potent glutamate receptor antagonist that is highly selective for the NR2B subunit of the NMDA receptor ; it has been shown to be neuroprotective in animal models of brain injury and ischemia . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Sections were processed and analyzed for [ 3H ] glutamate binding to NMDA receptors using receptor autoradiography and for mRNA for the zeta 1 ( NR 1 ) , epsilon 1 ( NR2A ) , and epsilon 2 ( NR2B ) subunits of the NMDA receptor using in situ hybridization . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Tonic facilitation of glutamate release by presynaptic NR2B containing NMDA receptors is increased in the entorhinal cortex of chronically epileptic rats . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The present review describes the role of glutamate in ischemic brain injury with an emphasis on the NR2B containing NMDA receptors . . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Synapses at P 2 contained substantial levels of NR 1 and NR2B and most GluR associated proteins , including SAP 102 , SynGAP , the chain of proteins from GluRs / SAP102 through GKAP / Shank / Homer and metabotropic glutamate receptors , and the adhesion factors , cadherin , catenin , neuroligin , and Nr CAM . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Comparative analysis of different competitive antagonists interaction with NR2A and NR2B subunits of N methyl D aspartate ( NMDA ) ionotropic glutamate receptor . ^^^ The antagonist bound conformation of the NR2A and NR2B subunits of N methyl D aspartate ( NMDA ) ionotropic glutamate receptor are modeled using the crystal structure of the DCKA ( 5 , 7 dichloro kynurenic acid ) bound form of the NR 1 subunit ligand binding core ( S1S2 ) . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
We have characterized several partial agonists at the NR1 / NR2B receptor and investigated the mechanisms underlying their reduced efficacy by introducing mutations in the glutamate binding site . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Among the 18 ionotropic glutamate receptor subunits identified in the mammalian central nervous system , five ( delta 1 , delta 2 , GluR 7 , chi 2 and NR3A , formerly called NMDAR L or chi 1 ) reportedly fail to form functional ion channels in heterologous expression systems . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NMDA receptor subunit gene expression in the rat brain : a quantitative analysis of endogenous mRNA levels of NR1Com , NR2A , NR2B , NR2C , NR2D and NR3A . ^^^ The present study was undertaken to examine the level of gene expression for the NR 1 , NR2A , NR2B , NR2C , NR2D and NR3A receptor subunits in isolated regions of rat brain using the nuclease protection assay . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
When coexpressed with NR 1 and NR2A in heterologous cells , NR3B suppressed glutamate induced current similarly to NR3A . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Remarkably , when co expressed in Xenopus oocytes , NR3A or NR3B co assembles with NR 1 to form excitatory glycine receptors that are unaffected by glutamate or NMDA , and inhibited by D serine , a co activator of conventional NMDARs . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Using the anti NR3A antibody in immunoprecipitation studies , we find that 9 . 7 + / 0 . 8 % of NR 1 , 8 . 7 + / 1 . 8 % of NR2A , and 5 . 0 + / 0 . 6 % of NR2B are associated with NR3A at postnatal day 10 . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
GT 1 7 neurons express mRNAs for various mouse NMDA receptor subunits ( zeta , 1 , epsilon 3 , epsilon 4 , and epsilon 2 , corresponding to the NR 1 , NR2C , NR2D , and NR2B rat subunits ) and increase their spontaneous release of GnRH when incubated in the presence of exogenous glutamate or NMDA . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The glutamate neurotransmitter system has been implicated in psychosis and schizophrenia , and so we looked for genetic association and measured gene expression in human DNA and brain samples , respectively , of the GRIN2B gene that codes for the NR2B protein . ^^^ The NR2B protein is a critical structural and functional subunit of the NMDA glutamate receptor . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
However , activity dependent phosphorylation of substrates at synapses was highly selective in that the glutamate receptor subunits NR2B and GluR 1 were poorly phosphorylated whereas PSD 95 and Stargazin , proteins implicated in the scaffolding and trafficking of AMPA receptors , were robustly phosphorylated . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The expression of several other synaptic proteins ( NR2B , glutamate receptors 2 / 3 , N ethylmaleimide sensitive factor ) was not affected by long term potentiation induction . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
The NMDA subtype of glutamate receptors are heteromeric complexes comprised of multiple subunits encoded by at least seven different genes ( NR 1 , NR2A 2D and NR3A 3B ) , and differential expression of these subunits alters the pharmacological and electrophysiological properties of NMDA receptors . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Expression of NR2A , NR2B , and NR2D was observed in all three cell types while NR3A was much more abundant in pre Botzinger complex interneurons , which belong to the rhythm generator of respiratory activity . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NR 1 , NR2A , NR2B , NR2C , NR2D and NR3A subunits showed clustered expression in cell processes , but NR3B was absent . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
Here , a soluble NR3A ligand binding domain ( NR3A S1S2 ) was constructed based on amino acid sequence alignments with other glutamate receptor ion channels and is expressed in Escherichia coli . ^^^ Glutamate , which binds to NR 2 subunits , also binds to NR3A , but with very low affinity ( Kd = 9 . 6 mm ) ; in contrast , binding of glutamate to NR 1 was not detectable even at a 300 mm concentration . ^^^
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
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Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA
Interacting proteins: Q8TCU5 and Q13224 Pubmed SVM Score :0.0
NA