| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.57323042 |
| Here we show that CaMKII directly binds to the NMDA receptor subunits NR 1 and NR2B . 0.57323042^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.71486125 |
| CaMKII binds NR2B by its catalytic site and by the autophosphorylation site binding pocket ( APBP ) , a non catalytic site . 0.71486125^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.55727719 |
| Autophosphorylated CaMKII binds directly to NMDAR subunits , co localizes with NMDARs in the postsynaptic density , and phosphorylates NR2B subunits at Ser 1303 . 0.55727719^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We report here that haloperidol causes an increase in the activity of CaMK 2 and a decrease in the density of immuno gold labelling for glutamate within the nerve terminals of asymmetrical synapses containing a perforated or nonperforated PSD . ^^^ These results are consistent with the hypothesis that the haloperidol induced increase in activity of CaMK 2 and the increase in glutamate release , as suggested by the decrease in presynaptic glutamate immunoreactivity , may ultimately lead to an increase in the number of synapses displaying a perforated PSD . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We have examined glutamate receptors ( GluRs ) as substrates for CaM K 2 because ( 1 ) they are colocalized in the PSD , ( 2 ) cloned GluRs contain consensus phosphorylation sites for protein kinases including CaM K 2 , and ( 3 ) several GluRs are regulated by other protein kinases . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In contrast , neither CaM K 2 nor PKA seems to mediate the metabotropic glutamate receptor action on IAHP . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| This selectively solubilized actin and spectrin while other prominent PSD proteins , such as CaMK 2 alpha , the AMPA and NMDA type glutamate receptors and GABA receptors , were not extracted at all . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| SMI 34 labeling of perikarya and dendrites was prevented by pretreatment with either the NMDA receptor antagonist APV or by the Ca2+ / calmodulin dependent protein kinase ( CaMK ) inhibitor KN 62 , but not by antagonists of AMPA / kainate or metabotropic glutamate receptors or by inhibitors of arachidonic acid metabolic pathways . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Ca2+ / calmodulin dependent protein kinase 2 ( CAMK 2 ) and one of its target , alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid ( AMPA ) , glutamate receptors have been shown to participate in both long term potentiation ( LTP ) in the hippocampus , and in spatial , as well as in a variety , of learning paradigms . ^^^ Taken together , these findings confirm and extend previous data suggesting that CAMK 2 and AMPA glutamate receptors in the hippocampus participate in the early phase of memory formation of an inhibitory avoidance learning . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Interestingly , KN 62 , which interferes with calcium calmodulin kinase ( CaM K ) activity , leads to a reduction of glutamate induced ERK activation and of CREB phosphorylation . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Our results suggest that CREB phosphorylation in neurons after ischemia and exposure to glutamate is induced by NMDA receptor gated calcium influx and subsequent activation of CaMK 2 4 and that CREB phosphorylation after metabolic stress might show a neuroprotective response through CRE mediated gene induction . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| A chronic ( 2 wk ) inactivation of N methyl D aspartate ( NMDA ) ionotropic glutamate receptors , L type voltage gated Ca ( 2+ ) channels , calmodulin , Ca ( 2+ ) / calmodulin dependent protein kinases II / IV ( CaMK II / IV ) , or protein kinase C ( PKC ) increased the number of cholinergic neurons ( to 15 24 % ) and induced ACh activity ( in 40 60 % of cells ) . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| A series of genes that were not reported before were up regulated by phenylalanine , including Ca ( 2+ ) / calmodulin dependent protein kinase , Brain type 2 ( CaMK 2 ) , Ras , P 38 MAP kinase , L voltage dependent calcium channel , some genes related to vesicle formation and transmitter release , some glutamate receptor subunits and glutamate transporters . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| These results indicate that activation of glutamate receptors triggers the release of purines from retinal cells by a mechanism involving calcium influx , CAMK 2 and the nitrobenzylthioinosine sensitive nucleoside transporter . ^^^ The regulation of adenosine release by glutamate receptors and CAMK 2 could have important consequences in the presynaptic control of glutamate release . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| A series of genes that were not reported previously were upregulated by phenylalanine , including Ca2+ / calmodulin dependent protein kinase , Brain type 2 ( CaMK 2 ) , ras , P 38 , L voltage dependent calcium channel , some genes related to vesicle formation and transmitter release , some glutamate receptor subunits and glutamate transporters . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Purified PKC 2 and endogenous CaMK 2 catalyzed the phosphorylation of 80 200 kDa proteins in the PSD and L glutamate ( or NMDA ) induced increase of ( + ) 5 [ 3H ] methyl 10 , 11 dihydro 5H dibenzo [ a , d ] cyclohepten 5 , 10 imi ne maleate ( [ 3H ] MK 801 ; open channel blocker for NMDA receptor / channel ) binding activity was significantly enhanced . ^^^ However , the pretreatment of PKC 2 and CaMK 2 catalyzed phosphorylation did not change the binding activity of L [ 3H ] glutamate , cis 4 [ 3H ] ( phosphonomethyl ) piperidine 2 carboxylate ( [ 3H ] CGS 19755 ; competitive NMDA receptor antagonist ) , [ 3H ] glycine , alpha [ 3H ] amino 3 hydroxy 5 methyl isoxazole 4 propionate , or [ 3H ] kainate in the PSD . ^^^ Pretreatment with PKC 2 and CaMK 2 catalyzed phosphorylation enhanced L glutamate induced increase of [ 3H ] MK 801 binding additionally , although purified cyclic AMP dependent protein kinase did not change L glutamate induced [ 3H ] MK 801 binding . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The role of several biological molecules in learning and memory are considered , for example , protein kinase C ( PKC ) , Ca ( ++ ) Calmodulin kinase 2 ( CaMKII ) , GAP 43 , and glutamate receptors . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Here we show that calcium independent CaMKII specifically binds to isolated postsynaptic densities ( PSDs ) , leading to enhanced phosphorylation of many PSD proteins including the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid ( AMPA ) type glutamate receptor . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Regulatory phosphorylation of AMPA type glutamate receptors by CaM KII during long term potentiation . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Preactivated recombinant alpha calcium calmodulin dependent multifunctional protein kinase 2 ( CaMKII * ) was perfused internally into CA 1 hippocampal slice neurons to test the effect on synaptic transmission and responses to exogenous application of glutamate analogues . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Ca2+ / CaM dependent protein kinase 2 ( CaM KII ) can phosphorylate and potentiate responses of alpha amino 3 hydroxyl 5 methyl 4 isoxazole propionate type glutamate receptors in a number of systems , and recent studies implicate this mechanism in long term potentiation , a cellular model of learning and memory . ^^^ In this study we have identified this CaM KII regulatory site using deletion and site specific mutants of glutamate receptor 1 ( GluR 1 ) . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Activation and Thr 286 autophosphorylation of calcium / calmodulindependent kinase 2 ( CaMKII ) following Ca2+ influx via N methyl D aspartate ( NMDA ) type glutamate receptors is essential for hippocampal long term potentiation ( LTP ) , a widely investigated cellular model of learning and memory . ^^^ Here , we show that NR2B , but not NR2A or NR 1 , subunits of NMDA receptors are responsible for autophosphorylation dependent targeting of CaMKII . ^^^ Autophosphorylation induces direct high affinity binding of CaMKII to a 50 amino acid domain in the NR2B cytoplasmic tail ; little or no binding is observed to NR2A and NR 1 cytoplasmic tails . ^^^ Specific colocalization of CaMKII with NR2B containing NMDA receptors in transfected cells depends on receptor activation , Ca2+ influx , and Thr 286 autophosphorylation . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Ca2+ / calmodulin dependent protein kinase 2 ( CaM KII ) regulates numerous physiological functions , including neuronal synaptic plasticity through the phosphorylation of alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid type glutamate receptors . ^^^ In COS 7 cells phosphorylation of transfected alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid type glutamate receptors by CaM KII , but not by protein kinase C , was blocked upon cotransfection with CaM KIIN . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The association of NMDA receptor subunits and CaMKII was assessed by immunoprecipitating PSD proteins with antibodies specific for NR2A / B and CaMKII : CaMKII coprecipitated with NR2A / B and NR 1 but not with other glutamate ionotropic receptor subunits , such as GluR 1 and GluR 2 3 . ^^^ Here we report that CaMKII binds to NR2A and NR2B subunits of NMDA receptors in PSD isolated from cortex and hippocampus . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| To examine in vivo the implication of CaMKII activity in synaptic plasticity , we used an animal model characterized by developmentally induced targeted neuronal ablation within the cortex and the hippocampus , and showing , at presynaptic level , molecular alterations leading to facilitation of glutamate release in hippocampal synapses ( methylazoxymethanol treated rats , MAM rats ) . ^^^ CaMKII dependent phosphorylation of NR2A and NR2B is decreased in animals characterized by hippocampal damage and impaired LTP . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The acute intrastriatal injection of the CaMKII inhibitor KN 93 ( 1 . 0 micrograms ) not only normalized the levodopa induced motor response alterations but also attenuated the D 1 and D 2 receptor mediated serine phosphorylation of NR2A and NR2B subunits , respectively ( p < 0 . 02 ) . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| LTP in the CA 1 field of the hippocampus requires activation of Ca2+ / calmodulin kinase 2 ( CaM KII ) , which phosphorylates Ser 831 in the GluR 1 subunit of the alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate glutamate receptor ( AMPA R ) , and this activation / phosphorylation is thought to be a postsynaptic mechanism in LTP . ^^^ Coexpression in HEK 293 cells of activated CaM KII with GluR 1 did not affect the glutamate affinity of the receptor , the kinetics of desensitization and recovery , channel rectification , open probability , or gating . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation of the glutamate receptor 1 ( GluR 1 ) subunit of AMPA receptors by protein kinase A ( PKA ) , protein kinase C ( PKC ) , and Ca2+ / calmodulin dependent protein kinase 2 ( CaMKII ) has been characterized extensively . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The level of antibody binding to the CamKII and the activity of CamKII were found to be increased after intravitreal injection of glutamate . ^^^ The glutamate mediated increases in CamKII were specific and blocked by 3 , 5 Dimethyl 1 adamantanamine ; 3 , 5 Dimethylamantadine ( Memantine ) , ( + / ) 2 Amino 5 Phosphopentonic ( AP 5 ) and 6 Cyano 7 Nitroquinoxaline 2 , 3 Dione ( CNQX ) but not with dl 2 Amino 3 Phosphono Propionic ( AP 3 ) . ^^^ The results indicate that the retinal neurotransmitter , glutamate , can regulate retinal CamKII activity through ionotropic but not metabotropic glutamate receptors . ^^^ A model in which cooperative interaction between NMDA and non NMDA glutamate receptors / ion channels is presented to explain the glutamate stimulated increases in CamKII activity in the retina . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Calcium influx through the N methyl d aspartate ( NMDA ) type glutamate receptor and activation of calcium / calmodulin dependent kinase 2 ( CaMKII ) are critical events in certain forms of synaptic plasticity . ^^^ We have previously shown that autophosphorylation of CaMKII induces high affinity binding to the NR2B subunit of the NMDA receptor ( Strack , S . , and Colbran , R . ^^^ Here , we show that residues 1290 1309 in the cytosolic tail of NR2B are critical for CaMKII binding and identify by site directed mutagenesis several key residues ( Lys ( 1292 ) , Leu ( 1298 ) , Arg ( 1299 ) , Arg ( 1300 ) , Gln ( 1301 ) , and Ser ( 1303 ) ) . ^^^ Phosphorylation of NR2B at Ser ( 1303 ) by CaMKII inhibits binding and promotes slow dissociation of preformed CaMKII . ^^^ Peptide competition studies imply a role for the CaMKII catalytic domain , but not the substrate binding pocket , in the association with NR2B . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Densin 180 differs from the previously identified CaMKII binding protein NR2B in that binding does not strictly require CaMKII autophosphorylation . ^^^ Binding of densin 180 and NR2B to CaMKII is noncompetitive , indicating different interaction sites on CaMKII . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The aims of this study were to investigate the involvement of ionotropic glutamate receptors , especially those of the NMDA subtype , and the requirement for Ca ( 2+ ) / calmodulin dependent protein kinase 2 ( CaMKII ) in these phenomena . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| As ionotropic glutamate receptors are known substrates of CAMKII , this study set out to determine if the protein levels of ionotropic glutamate receptors in the rdta mouse retina are also affected . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Calcium and calmodulin dependent protein kinase 2 ( CaMKII ) and glutamate receptors are integrally involved in forms of synaptic plasticity that may underlie learning and memory . ^^^ Here we show that regulated CaMKII interaction with two sites on the NMDA receptor subunit NR2B provides a mechanism for the glutamate induced translocation of the kinase to the synapse in hippocampal neurons . ^^^ CaMKII NR2B interaction may be prototypical for direct activation of a kinase by its targeting protein . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In the CA 1 region , induction of LTP enhances the function of postsynaptic alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate receptors ( AMPARs ) because of the Ca2+ calmodulin kinase 2 ( CaMKII ) dependent phosphorylation of this subtype of glutamate receptor . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The above results indicate that intra amygdaloid injection of KA produces excitatory signals for ipsilateral CA 3 neurons in the hippocampus and that subsequently increased levels of CaMKII in postsynaptic neurons induce neuronal injury via phosphorylation of N methyl D aspartate type glutamate receptor . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Group 1 metabotropic glutamate receptors control phosphorylation of CREB , Elk 1 and ERK via a CaMKII dependent pathway in rat striatum . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In contrast , 4 months after induction of diabetes NR2B subunit immunoreactivity , CaMKII and Tyr dependent phosphorylation of the NR2A / B subunits of the NMDA receptor were reduced and alphaCaMKII autophosphorylation and its association to the NMDA receptor complex were impaired in STZ rats compared with age matched controls . ^^^ Insulin treatment prevented the reduction in kinase activities , NR2B expression levels , CaMKII NMDA receptor association and NMDA currents . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Activation of CaMKII has been shown to phosphorylate the glutamate receptor 1 subunit of the AMPA receptor ( AMPAR ) , thereby affecting some of the properties of the receptor . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Calcium / calmodulin dependent protein kinase type 2 ( CaMKII ) and NMDA type glutamate receptor ( NMDAR ) are neuronal proteins involved in learning and memory . ^^^ Sequence determinants on the NR2A and NR2B subunits of NMDA receptor responsible for specificity of phosphorylation by CaMKII . ^^^ CaMKII binds to the NR2B subunit of NMDAR in more than one mode , a stable association involving a noncatalytic site on CaMKII and an enzyme substrate mode of interaction by its catalytic site . ^^^ We find that residues 1292 1297 of NR2B enhance the affinity of the catalytic site mediated binding of CaMKII to the minimal phosphorylation site motif , 1298 1308 of NR2B , as evident from measurements of K ( m ) values for phosphorylation . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In cultures , glutamate induced thickening of PSDs and the accumulation of CaMKII on PSDs are reversed within 5 min of removal of glutamate and Ca ( 2+ ) from the extracellular medium . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Previous studies have shown that multifunctional calcium / calmodulin dependent protein kinase 2 ( CaMKII ) and one of its substrates , the glutamate receptor , are key players in experience driven synaptic plasticity in several areas of the central nervous system ( CNS ) . ^^^ To determine if CaMKII and the glutamate receptor are regulated by visual activity in the retina , we compared dark reared ( DR ; 1 week ) rats with control rats raised in a diurnal light dark cycle ( LD ) , at the following ages : postnatal day 12 ( P12d ) , 2 month ( 2m ) and 6 month ( 6m ) old . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Further , the activation of glutamate receptors increases the association of p 35 and p 39 with CaMKIIalpha , and the inhibition of CaMKII activation diminishes this effect . ^^^ The glutamate mediated increase in association of p 35 and CaMKIIalpha is mediated in large part by NMDA receptors , suggesting that cross talk between the Cdk 5 and CaMKII signal transduction pathways may be a component of the complex molecular mechanisms contributing to synaptic plasticity , memory , and learning . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Calcium / calmodulin dependent protein kinase 2 ( CaMKII ) and the ionotropic glutamate receptors ( iGluRs ) have been shown to be pivotal in the maturation of synapses during development of the central nervous system . ^^^ The patterns of CaMKII and NR1 / NR2A were better correlated than were CaMKII and NR2B , or CaMKII and GluR 1 . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Ca ( 2+ ) influx through the N methyl d aspartate ( NMDA ) type glutamate receptor leads to activation and postsynaptic accumulation of Ca ( 2+ ) / calmodulin dependent protein kinase 2 ( CaMKII ) and ultimately to long term potentiation , which is thought to be the physiological correlate of learning and memory . ^^^ This autophosphorylation reduces the off rate to a level ( t ( 12 ) = approximately 23 min ) that is similar to that observed for dissociation of the T286D mutant CaMKII ( t ( 12 ) = approximately 30 min ) from spines after its glutamate induced accumulation ( Shen , K . , Teruel , M . ^^^ The NMDA receptor also serves as a CaMKII docking site in dendritic spines with high affinity binding sites located on its NR 1 and NR2B subunits . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The following results were observed : ( 1 ) CaMKII alpha immediately became autophosphorylated after 3 min ischemia , at the same time , there is a dramatic and sustained translocation of CaMKII alpha from cytosolic fraction to synaptic fraction ; ( 2 ) CaMKII alpha translocated to post synaptic density and targeted N methyl D aspartate receptor subunit 2B ( NR2B ) which was serine phosphorylated by active CaMKII alpha ; ( 3 ) serine phosphorylation of NR2B could not only inhibit the formation of CaMKII alpha NR2B complexes but also promote the dissociation of the preformed complexes when ischemic time was prolonged . ^^^ These results suggest that phosphorylation of NR2B can influence the channel properties of NR2B , and the dissociation of the CaMKII alpha NR2B complexes may be a negative feedback mechanism during longer time cerebral ischemia . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The effect of environmental enrichment on NR 1 and BDNF gene expression was specific to Pb2+ exposed animals and was present in the absence of changes in the NR2B subunit of the N methyl D aspartate receptor , GluR 1 , alpha CamKII , or PSD 95 gene expression measured in the same animals . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Recent evidence shows that calcium / calmodulin dependent kinase 2 ( CaMKII ) is also upregulated early in the process of central sensitization and that several types of ionotropic glutamate receptors become phosphorylated . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The role of NMDA glutamate receptors , PKA , MAPK , and CAMKII in the hippocampus in extinction of conditioned fear . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Consistently , we show that glutamate receptor mutants also have a higher number of T bars ; this increase is suppressed by postsynaptic activation of CaMKII . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Here we show that SAP 97 is enriched at the postsynaptic density where it co localizes with both ionotropic glutamate receptors and downstream signaling proteins such as Ca2+ / calmodulin dependent protein kinase 2 ( CaMKII ) . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Mutation of Glu 60 , a residue in the ATP binding region of CaMKII , to glycine exerts different effects on phosphorylation of two peptide substrates , syntide and NR2B ( N methyl D aspartate receptor subunit 2B ) 17 mer . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Interactions with various subunits of the NMDA ( N methyl D aspartate ) subtype of glutamate receptor have attracted the most attention , but binding of CaMKII to cytoskeletal and several other regulatory proteins has also been reported . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| As to subcellular neurochemical mechanisms of sensitization , the activation of three main cascades is indispensable , 1 ) D 1 dopamine ( DA ) receptors / PKA / phospho 34Thr DARPP 32 / PP 1 cascade activated by psychostimulant induced enhancement of DA release in the accumbens , 2 ) NMDA receptors and CaM KII activated by enhanced release of glutamate , 3 ) activation of MAP kinase cascade by BDNF and beta 1 subunit of G protein . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Autophosphorylated calcium / calmodulin dependent protein kinase 2 alpha ( CaMKII alpha ) reversibly targets to and phosphorylates N methyl D aspartate receptor subunit 2B ( NR2B ) in cerebral ischemia and reperfusion in hippocampus of rats . ^^^ Secondly , KN 62 also inhibited the autophosphorylation of CaMKIIalpha , NR2B serine phosphorylation and the binding of CaMKIIalpha to NR2B but had no effect on the translocation of CaMKII . ^^^ Thirdly , our results indicated the concomitant phosphorylation and dephosphorylation of CaMKII and NR2B following ischemia or longer reperfusion . ^^^ Moreover , the dissociation of CaMKII from NR2B had the same trend as that of the return of CaMKII to cytosol . ^^^ All these data imply the close relationships between CaMKII and NR2B during ischemia and reperfusion , namely , CaMKII might act as an amplifier of detrimental cellular calcium signal regulated by NMDA receptors when becoming autophosphorylated and targeting to NR2B ; conversely , autophosphorylated CaMKII could modulate NMDA receptor channel properties by phosphorylating NR2B . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NGF significantly increased the depolarization induced activation of Ca2+ / calmodulin dependent protein kinase 2 ( CaMKII ) and the subsequent phosphorylation of synapsin 1 in the absence of external Ca2+ and the NGF effect on evoked glutamate release was inhibited by the CaMKII inhibitors KN 93 and CaMKII 281 309 peptide but not by the MAP kinase inhibitor PD 98059 . ^^^ Thus , the effect of NGF on evoked glutamate release is linked to an increase in [ Ca2+ ] 1 contributed by both Ca2+ entry and mobilization from InsP 3 sensitive , ryanodine sensitive and mitochondrial stores and to the subsequent activation of CaMKII . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The increased levels of extracellular glutamate , NMDA receptor zeta subunit ( NR 1 ) mRNA , NMDA receptor epsilon 1 subunit ( NR2A ) protein , phosphorylated Ca ( 2+ ) / calmodulin kinase 2 ( p CaMKII ) protein , c fos mRNA , c Fos protein , are observed in the specific brain areas of mice and / or rats showing signs of naloxone precipitated withdrawal . ^^^ The activation of CaMKII and increased expression of c Fos protein in the brain of animals with naloxone precipitated withdrawal syndrome are prevented by NMDA receptor antagonists , whereas the increased levels of extracellular glutamate are not prevented by them . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Treatment of neurons with 5 microm glutamate stimulated CaMKII phosphorylation of nNOS at Ser ( 847 ) , whereas excitotoxic concentrations of glutamate , 100 and 500 microm , induced Ser ( 847 ) PO ( 4 ) dephosphorylation by protein phosphatase 1 . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : PSD 95 competes with CaMKII to bind to NR2B during ischemia and reperfusion in rat hippocampus . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The membrane expression of key molecules in memory consolidation , such as the NR 1 and NR2B subunits of the N methyl D aspartate ( NMDA ) receptor , Ca2+ / calmodulin dependent protein kinase 2 ( CaMKII ) and protein phosphatase 1 ( PP 1 ) was measured . ^^^ In untrained rats , MDMA reduced NR 1 and NR2B protein levels , membrane CaMKII levels and enzyme activity , and enhanced PP 1 levels and activity . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Further , CaMKII dependent SAP 97 Ser 39 phosphorylation determined a redistribution of the glutamate receptor subunit ( GluR 1 ) of the AMPA receptor . ^^^ In conclusion , our data show that CaMKII dependent SAP 97 Ser 39 phosphorylation regulates the association of SAP 97 with the postsynaptic complex , thus providing a fine molecular mechanism responsible for the synaptic delivery of SAP 97 interacting proteins , i . e . ionotropic glutamate receptor subunits . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Here we report that KN 93 , a membrane permeable CaMKII inhibitor , blocked glutamate induced increases in the frequency of miniature excitatory postsynaptic currents ( mEPSCs ) and the number of presynaptic functional boutons in cultured hippocampal pyramidal neurons . ^^^ In addition , presynaptic injection of the membrane impermeable CaMKII inhibitor peptide 281 309 blocked synaptic plasticity induced by tetanus , glutamate , or NO / cGMP pathway activation as expressed by long lasting increases in EPSC amplitude and functional presynaptic boutons . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Co addition of the protein kinase C ( PKC ) inhibitor GF109203X and the Ca ( 2+ ) calmodulin dependent kinase 2 ( CaMKII ) inhibitor KN 93 blocked the ability of glutamate and carbachol to increase the association of GRK 2 with mGluR1a . ^^^ However , unlike mGluR1a , glutamate stimulated association of GRK 2 with mGluR1b was not reduced by PKC / CaMKII inhibition . ^^^ These results demonstrate that the internalization of mGluR1a , triggered homologously by glutamate or heterologously by carbachol , is PKC / CaMKII , GRK 2 , arrestin , and clathrin dependent and that PKC / CaMKII activation appears to be necessary for GRK 2 to associate with mGluR1a . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The present findings also suggest that glutamate agonist induced activation of CaM KII in the VTA plays a critical role in the behavioral and neuronal plasticity induced by repeated cocaine injections . . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Development was marked by substantial decreases in NR2B and SAP 102 and increases in NR2A , PSD 95 , AMPA receptors , and CaMKII . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Protein synthesis dependent late phase LTP ( L LTP ) in the hippocampus requires calcium influx through the NMDA type glutamate receptor ( NMDA R ) to activate CaMKII as well as concomitant inhibition of PP 1 mediated by protein kinase A . ^^^ |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| In addition , the autocamtide 2 related inhibitory peptide ( AIP ) , a specific inhibitor of CaMKII , was used to determine the involvement of CaMKII in glutamate induced RGC 5 cell death . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| However , phosphorylation of the CaMKII PP 1 substrate , Ser 831 in the glutamate receptor GluR 1 subunit , was increased only after sustained ( 9 20 months ) dopamine depletion . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Here , we show that in transgenic mice overexpressing the human AD gene APPswe ( Tg 2576 ) , safflower oil induced n 3 PFA deficiency caused a decrease in N methyl D aspartate ( NMDA ) receptor subunits , NR2A and NR2B , in the cortex and hippocampus with no loss of the presynaptic markers , synaptophysin and synaptosomal associated protein 25 ( SNAP 25 ) . n 3 PFA depletion also decreased the NR 1 subunit in the hippocampus and Ca2+ / calmodulin dependent protein kinase ( CaMKII ) in the cortex of Tg 2576 mice . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We base our models on published biochemical data and experiments on the activity dependent movement of a glutamate receptor , AMPAR , and a calcium dependent kinase , CaMKII . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We show that CaMKIIalpha is associated with the CaMKII binding proteins densin 180 , the N methyl D aspartate receptor NR2B subunit , and alpha actinin in postsynaptic density enriched rat brain fractions . ^^^ Similar amounts of Thr 286 autophosphorylated CaMKIIalpha holoenzyme [ P T 286 ] CaMKII bind to alpha actinin 2 as bind to NR2B ( residues 1260 1339 ) or to densin 180 ( residues 1247 1495 ) in glutathione agarose cosedimentation assays , even though the CaMKII binding domains share no amino acid sequence similarity . ^^^ Like NR2B , alpha actinin 2 binds to representative splice variants of each CaMKII gene ( alpha , beta , gamma , and delta ) , whereas densin 180 binds selectively to CaMKIIalpha . ^^^ Soluble alpha actinin 2 does not compete for [ P T 286 ] CaMKII binding to immobilized densin 180 or NR2B . ^^^ However , soluble densin 180 , but not soluble NR2B , increases CaMKII binding to immobilized alpha actinin 2 by approximately 10 fold in a PDZ domain dependent manner . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NR2B inhibits both the Ca ( 2+ ) / calmodulin dependent and autonomous activities of CaMKII by a mechanism that is competitive with autocamtide 2 substrate , non competitive with syntide 2 substrate , and uncompetitive with respect to ATP . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Extensive studies have shown that long term potentiation ( LTP ) of the excitatory postsynaptic current ( EPSC ) through glutamate receptors is induced by activation of N methyl D asparate receptor ( NMDA R ) the coincidence detector and Ca ( 2+ ) / calmodulin dependent protein kinase 2 ( CaMKII ) . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Switching synaptic NR2B containing NMDA Rs that bind CaMKII with high affinity with those containing NR2A , a subunit with low affinity for CaMKII , dramatically reduces LTP . ^^^ Finally , driving into synapses NR2B with mutations that reduce association to active CaMKII prevents LTP . ^^^ We conclude that association between active CaMKII and NR2B is required for different forms of synaptic enhancement . ^^^ The switch from NR2B to NR2A content in synaptic NMDA Rs normally observed in many brain regions may contribute to reduced plasticity by controlling the binding of active CaMKII . . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation is catalyzed by Ca ( 2+ ) / calmodulin dependent protein kinase 2 ( CaMKII ) activity , as a result of activation of NR2B containing N methyl D aspartate subtype of glutamate receptors ( NMDARs ) during ischemia . ^^^ Specific blockade of NMDAR / CaMKII ASIC coupling may reduce neuronal death after ischemia and other pathological conditions involving excessive glutamate release and acidosis . . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Inhibition of CaMKII by m AIP did not affect expression but significantly enhanced the release of BDNF from glutamate challenged cells . ^^^ A novel mechanism for neuroprotection is proposed for the CaMKII inhibitor , AIP , which appears to protect RGC 5 cells from cytotoxicity by enhancing the release of BDNF from glutamate challenged cells . . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Concomitant with the synaptic accumulation of alpha CaMKII in response to D 4 receptor activation , a D 4 induced increase in the CaMKII phosphorylation of alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid ( AMPA ) receptor glutamate receptor 1 ( GluR 1 ) subunits and the amplitude of AMPA receptor mediated excitatory postsynaptic currents was also observed . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Transition from reversible to persistent binding of CaMKII to postsynaptic sites and NR2B . ^^^ Both molecules , the NMDA receptor subunit NR2B and Ca2+ / calmodulin ( CaM ) dependent protein kinase 2 ( CaMKII ) , are strongly implicated in mediating synaptic plasticity . ^^^ We have identified two surface regions of CaMKII involved in short lived and long term interactions with NR2B . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Here we show that variability of human memory performance is related to variability in genes encoding proteins of this signaling cascade , including the NMDA and metabotrobic glutamate receptors , adenylyl cyclase , CAMKII , PKA , and PKC . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| This increase in Ser 19 phosphorylation was associated with enhanced TH activity and was due , in part , to glutamate receptor mediated calcium influx and possibly calcium / calmodulin dependent protein kinase 2 ( CaMKII ) activation . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| Glutamate or NMDA stimulation induced CaMKII autophosphorylation over a time course that mirrored the time course of p 35 degradation . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| We show that the calcium dependent effect on postsynaptic quantal size is mediated by group 1 metabotropic glutamate receptors , acting via CaMKII ( Ca2+ / calmodulin dependent protein kinase 2 ) and PKC . ^^^ |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13555 and Q13224 |
Pubmed |
SVM Score :0.0 |
| NA |
|