Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.58058027
Immunoprecipitation results revealed that tyrosine phosphorylation of NR2A and NR2B as well as the association of phosphorylated FAK with NR2A and NR2B was increased with LTP induction . 0.58058027^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Glutamate was a full agonist at both receptors , having a modestly but statistically significant ( p < 0 . 002 ) higher affinity for the NR2B than the NR2A containing receptor ( microscopic Kd [ mKd ] = 0 . 76 and 0 . 43 microM , respectively ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Because N methyl D aspartate ( NMDA ) glutamate ionotropic receptors are involved in the occurrence of audiogenic seizures , we investigated by in situ hybridization methods , the expression of the different subunits ( NR 1 , NR2A , NR2B , NR2C ) of this receptor in the central nucleus of the inferior colliculus ( IC ) , a main relay of the auditory pathways . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Application of 500 microM picrotoxin for two days led to decreases in the messenger RNA levels for the N methyl D aspartate receptor subunits , NR2A and NR2B , and for the non N methyl D aspartate receptor subunits , glutamate receptors 1 and glutamate receptors 2 , to about 50 % of the levels seen in control cultures . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We used in situ hybridization to examine the effect of removal of the glutamatergic input to the striatum by unilateral frontal cortical ablation on the expression of genes encoding subunits from three families of glutamate receptors : N methyl D aspartate receptors ( NMDAR 1 , NMDAR2A , and NMDAR2B ) ; alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionate ( AMPA ) receptors ( GluR 1 4 , flip and flop splice variants ) ; and metabotropic receptors ( mGluR 1 5 ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Stimulation by histamine was dependent on the concentration of agonist used to activate NR1A / NR2B receptors and was seen with high but not low concentrations of NMDA and glutamate . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the present work we report the localization of the gene encoding the human NMDAR2B receptor subunit ( called GRIN2B for glutamate receptor , ionotropic , N methyl D aspartate 2B ) to chromosome 12p12 by in situ hybridization and somatic cell hybrids . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Transcript levels of the NMDA receptor genes NMDAR 1 , NR2A , NR2B , NR2C , and NR2D and a glutamate binding protein ( GBP ) were not altered by kindling . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At NR1A / NR2B receptors , in the presence of a saturating concentration of glycine , the magnitude of spermine stimulation was dependent on the concentration of NMDA or glutamate . ^^^ Spermine induced a small ( 1 . 2 1 . 5 fold ) decrease in the affinity of NR1A / NR2B receptors for NMDA and glutamate . ^^^ Endogenous polyamines could act as a bidirectional gain control at some native NMDA receptors containing the NR1A and NR2B subunits , by dampening the response to low concentrations of glutamate and enhancing the response to high concentrations of glutamate . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Mutation of D 669 to asparagine ( D669N ) , alanine ( D669A ) , or glutamate ( D669E ) abolished the `` glycine independent ' ' form of spermine stimulation in heteromeric NR1A / NR2B receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The rank order of potency was quinolinic acid < NMDA < homoquinolinic acid < or = glutamate for the NR 1 + NR2A and NR 1 + NR2B combinations whereas for NR 1 + NR2C it was quinolinic acid < < < < homoquinolinic acid < NMDA < or = glutamate . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We further characterized the glutamate site assembled from NR1a , NR2A , and NR2B NMDA receptor subunits using L [ 3H ] glutamate and [ 3H ] CGP 39653 binding assays . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Changes of NMDA receptor subunit ( NR 1 , NR2B ) and glutamate transporter ( GLT 1 ) mRNA expression in Huntington ' s disease an in situ hybridization study . ^^^ The distribution of NMDA receptor subunit ( NR 1 , NR2B ) and glia bound glutamate transporter ( GLT 1 ) mRNAs was investigated in postmortem brains of Huntington ' s disease ( HD ) patients and controls by means of in situ hybridization using radiolabeled deoxyoligonucleotides . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Molecular determinants of agonist discrimination by NMDA receptor subunits : analysis of the glutamate binding site on the NR2B subunit . ^^^ Here , we demonstrate that the glutamate binding site is located on the homologous regions of the NR2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
With the advent of powerful new microchemical tools and molecular genetic methods , three new classes of proteins have been identified in the PSD at glutamatergic synapses : the PSD 95 family , the NR2B subunit of the NMDA type glutamate receptor , and densin 180 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have investigated the developmental change in NMDA receptor affinity for both glutamate and glycine in acutely dissociated parietal cortex neurons of the rat , together with the expression during ontogeny of NR2A and NR2B mRNA and protein . ^^^ Whereas there is little change in NMDA receptor glutamate affinity with age , a population of NMDA receptors emerges in 14 and 28 d old animals with a markedly reduced affinity for glycine ( mKD = approximately 800 nM ) and a reduced sensitivity to the NR2B subunit selective NMDA antagonist ifenprodil . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , the numbers of glycine binding NR 1 and glutamate binding NR 2 subunits in the NMDA receptor hetero oligomer were determined by coexpressing the wild type ( wt ) NR 1 with the low affinity mutant NR 1 ( Q387K ) , and the wt NR2B with the low affinity mutant NR2BE387A , subunits in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Specific binding , defined with a new NR2B specific antagonist , Ro 04 5595 [ 1 ( 4 chlorophenyl ) 2 methyl 6 methoxy 7 hydroxy 1 , 2 , 3 , 4 tetrahydroisoqu inoline ] , at 10 microM , was fully inhibited by several compounds with the following rank order of affinities Ro 25 6981 > CP 101 , 606 > Ro 04 5595 = ifenprodil > > eliprodil > haloperidol > spermine > spermidine > MgCl 2 > CaCl 2 and partially inhibited by competitive glutamate recognition site antagonists . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Previous investigations utilizing 125I MK 801 binding assays with recombinant receptors distinguished certain structural determinants on the NR2B subunit for these two drugs , with glutamate 201 being critical for haloperidol sensitivity and arginine 337 being important for ifenprodil block . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Differential effects of electroconvulsive shock on the glutamate receptor mRNAs for NR2A , NR2B and mGluR5b . ^^^ In the dentate gyrus , such treatment elevated the mRNAs for the NMDA subunits NR2A and NR2B , but it reduced the mRNA for the metabotropic glutamate receptor mGlu5b . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The main glutamate receptor subunits detected in IS cells were GluR 1 flop and GluR 2 flop , GluR 5 and GluR 6 , and NR2B and NR2D for the alpha amino 3 hydroxyl 5 methyl 4 isoxazolepropionic acid ( AMPA ) , kainate and N methyl D aspartic acid ( NMDA ) subtypes , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This study examined the effects on rat behaviour of antagonists acting at various sites on the N methyl D aspartate ( NMDA ) receptor complex , i . e . the glutamate recognition site ( CPP ) , ion channel ( dizocilpine ) , glycine recognition site [ ( + ) HA 966 ] and the NR2B subunit selective compound ifenprodil . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The effect of treatment with the D 1 dopamine receptor agonist SKF 38393 on the expression of metabotropic glutamate receptor 1 , 3 , 4 and 5 receptor subtypes and of the glutamate N methyl D aspartate ionotropic receptor subunits NRI , NR2A and NR2B was analysed using in situ hybridization . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we investigated the co expression of the neurotrophin 4 / brain derived neurotrophic factor receptor TrkB with the N methyl D aspartate glutamate receptor subunits NR 1 C1 , NR2A and NR2B , on postnatal days 10 and 22 and in the adult rat primary visual cortex . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To investigate the role of NMDA receptor subunits in the neuroendocrine functions of the magnocellular neuroendocrine cells of the hypothalamus , we examined the effects of osmotic stimulation on the protein expression of the NMDA receptor subunits , NR 1 and NR2B , important in binding glycine and glutamate , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Quantitative in situ hybridization revealed that following the induction of hippocampal long term potentiation ( LTP ) in the dentate gyrus of freely moving rats , specific increases in the expression of the NR2B subunit of the N methyl D aspartate ( NMDA ) receptor and mGluR1c , a short splice variant of the metabotropic glutamate receptors that are linked intracellularly to phospholipase C ( PLC ) and protein kinase C ( PKC ) , were seen in the postsynaptic dentate granule cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We measured the glutamate induced increase of calcium concentration in fibroblasts expressing one of the NR 1 splice forms and the NR2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The time course of the increase in NR2B mRNA most closely followed the increase in glutamate stimulated changes in the magfura 2 signal and neuronal injury . ^^^ Therefore , the increases in the glutamate stimulated [ Ca ( 2+ ) ] ( 1 ) responses and NMDA receptor subunit mRNA levels ( especially NR2B ) are likely involved in the development of susceptibility to excitotoxicity in cultured rat forebrain neurons . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Because native NR2A and NR2B containing receptors are pharmacologically distinct , it should be possible to develop NR2A and NR2B selective glutamate site antagonists . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This neurotoxic effect could be totally prevented by preincubation with an N methyl D aspartate receptor subunit 2B ( NR2B ) specific antagonist , NR2B antibodies , and an unspecific N methyl D aspartate receptor blocker and was partially blocked with an NR2A specific antagonist but not with NR2A antibodies or alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate receptor and metabotropic glutamate receptor antagonists . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
L glutamate , NMDAR2B and the enzyme glutamine synthetase were identified by using monoclonal antibodies . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Alpha actinin ( alpha actinin 2 ) is a protein which links the NR 1 and NR2B subunits of N methyl D aspartate ( NMDA ) glutamate receptors to the actin cytoskeleton . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Allosteric interactions with polyamines , Mg ( 2+ ) , Zn ( 2+ ) , glutamate , glycine , and their antagonists were consistent with NMDA receptors with NR2B subtype pharmacology . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At immature synapses , a high probability of glutamate release ( Pr ) was correlated with the expression of postsynaptic NMDA ( N methyl D aspartate ) receptors containing the NR2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we show that regulated CaMKII interaction with two sites on the NMDA receptor subunit NR2B provides a mechanism for the glutamate induced translocation of the kinase to the synapse in hippocampal neurons . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
E2F1 ( + / + ) and ( / ) cultures were comparable , in that they contained similar neuronal densities , responded with similar increases in intracellular calcium concentration ( [ Ca ( 2+ ) ] 1 ) to glutamate receptor agonists , and showed similar NMDA receptor subunit mRNA expression levels for NR 1 , NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 ( F639A ) did not alter the agonist potency of glutamate but did produce a leftward shift in the glycine concentration response for receptors containing NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RESULTS : Glutamate receptor expression was lower at both transcriptional ( NMDAR 1 , NMDAR2B , NMDAR2C , gluR 1 , gluR 3 , and KA 2 subunit mRNAs ) and posttranscriptional ( [ ( 3 ) H ] ifenprodil and [ ( 3 ) H ] MDL 105 , 519 binding to polyamine and glycine sites of the NMDA receptor ) levels in the thalamus in patients with schizophrenia than in comparison subjects , but differences were most prominent in nuclei with reciprocal projections to limbic regions . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , the effect of Con G on recombinant NMDA receptors carrying point mutations within the glycine and glutamate binding pockets of the NR 1 and NR2B subunits was studied using whole cell voltage clamp recording from cRNA injected Xenopus oocytes . ^^^ Homology based molecular modeling of the glutamate binding region based on the known structure of the glutamate binding site of the AMPA receptor protein GluR 2 suggests how selected amino acid side chains of NR2B might interact with specific residues of Con G . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Furthermore , our biochemical experiments showed that enrichment significantly increased protein levels of GluR 1 , NR2B , and NR2A subunits of glutamate receptors in both wild type and NR2B Tg mice . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
It has also been demonstrated that the level of an ionotropic N methyl d aspartate 2B subunit ( GRIN2B ) of the glutamate receptor tends to increase after subchronic administration of clozapine , suggesting that GRIN2B may play an active role in the pathogenesis of schizophrenia and the function of clozapine medication . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The NR2B subunit of glutamate receptors as a potential target for relieving chronic pain : prospects and concerns . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Lithium protection against glutamate excitotoxicity in rat cerebral cortical neurons : involvement of NMDA receptor inhibition possibly by decreasing NR2B tyrosine phosphorylation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Whereas PS potentiated NMDA , glutamate , and glycine induced currents of NR1 / NR2A and NR1 / NR2B receptors , it was inhibitory at NR1 / NR2C and NR1 / NR2D receptors . 3 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Glutamate receptors and persistent pain : targeting forebrain NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We quantitatively defined the contributions of carboxyl terminal residues to binding selectivity of the prototypic interactions of the PDZ domains of postsynaptic density protein 95 ( PSD 95 ) and its homolog synapse associated protein 90 ( SAP 102 ) with the NR2b subunit of the N methyl d aspartate type glutamate receptor . ^^^ Prominent were a requirement for glutamate or glutamine at P ( 3 ) and for valine at P ( 0 ) for high affinity binding and a preference for threonine over serine at P ( 2 ) , in the context of the last 11 residues of the NR2b COOH terminus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Computer simulation of the three dimensional structure of the glutamate site of the NR2B subunit of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The immunohistochemical localization of the NMDA glutamate receptor subunits NR 1 , NR2A , and NR2B was investigated in the rat vestibular periphery at the light and electron microscopy level using specific antipeptide antibodies . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The increase in NR2B tyrosine phosphorylation was abolished by intrathecal pretreatment with genistein , a tyrosine kinase inhibitor ; PP 2 , an Src family tyrosine kinase inhibitor ; AIDA , a group 1 metabotropic glutamate receptor antagonist ; L 733 , 060 , an NK 1 tachykinin receptor antagonist , and chelerythrine , a protein kinase C inhibitor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In cultures treated with vehicle and AraC , glutamate induced neurotoxicity was mediated through stimulation of the NR 1 NR2B subtype of NMDA receptors , because it was blocked by the NMDA receptor antagonist MK 801 and the NR 1 NR2B selective receptor antagonist ifenprodil . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We present new homology based models of the glutamate binding site ( in closed and open forms ) of the NMDA receptor NR2B subunit derived from 10 ray structures of the water soluble AMPA sensitive glutamate receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In this study , the protein expression levels of several ionotropic glutamate receptor subunits ( N methyl D aspartate ( NMDA ) subunits NR 1 , NR2A , NR2B , and alpha amino 3 hydroxy 5 methyl 4 isoxazoleproprionic acid ( AMPA ) receptor subunits GluR 1 , GluR 2 , GluR 4 ) were quantified in particulate preparations from rat spinal cord at various ages after birth . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
These results suggest that big dynorphin induced nociceptive behavior is mediated through the activation of the NMDA receptor ion channel complex by acting on the NR2B subunit and / or the polyamine recognition site but not on the glycine recognition site , and does not involve opioid , non NMDA glutamate receptor mechanisms or tachykinin receptors in the mouse spinal cord . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The glutamate effects were enhanced by glycine application and were prevented by the NMDA receptor blocker MK 801 , as well as by the NR2B selective antagonist ifenprodil . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
By analyzing patch clamp recordings of one channel activated by glutamate , we determined the burst structure and open probability for recombinant rat NR1 / NR2B receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The recombinant AptNR1 / NR2B receptors produced robust currents after stimulation with glutamate or NMDA in the presence of glycine . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Pentamidine inhibited a constitutively active NR 1 ( T648A ) / NR2B NMDA receptor in a manner similar to its inhibition of a glutamate gated wild type NMDA receptor , but different from its inhibition of constitutively active delta 2 ( A654T ) receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The neuroprotective effects of lithium , a mood stabilizer , against glutamate induced excitotoxicity in rat cortical neurons were associated with a decrease in Tyr 1472 phosphorylation of the N methyl D aspartate ( NMDA ) receptor NR2B subunit and a loss of receptor activity . ^^^ A Src kinase inhibitor , SU 6656 , and an NR2B antagonist , ifenprodil , partially blocked glutamate excitotoxicity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In particular , NMDA receptor antagonists that selectively target the NR2B subunit and antagonists of the metabotropic glutamate receptor mGluR 5 appear to hold promise and deserve future attention . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The activity of the N methyl D aspartate ( NMDA ) receptor , a subclass of ionotropic glutamate receptor , is modulated by a complex network of phosphorylation and dephosphorylation . 1 investigated the relative extent of tyrosine phosphorylation of NMDA receptor subunit 2A ( NR2A ) and 2B ( NR2B ) subunits in the rat forebrain postsynaptic density ( PSD ) fraction . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition , dorsocaudal brain stem tissue lysates were harvested at 1 h , 6 h , 1 day , 3 days , 7 days , 14 days , and 30 days of SH and IH and analyzed for NR 1 , NR2A , and NR2B NMDA glutamate receptor expression by immunoblotting . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In studying glutamate receptor regulation of sgk expression , we found that blockade of N methyl d aspartate ( NMDA ) receptors in general , and the NR2B subunit in particular both effectively blocked enrichment induced spatial learning facilitation , but they did not block enrichment induced sgk expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunofluorescent analysis was used to investigate the existence and location of glutamate , glutamate receptor ( NR2B ) , and glutamate transporter ( GLT 1 ) in mouse and human sperm . ^^^ In addition , reverse transcription polymerase chain reaction technique and sequencing analysis revealed that glutamate transporters ( GLT 1 and EAAC 1 ) and ionotropic glutamate receptors ( NR 1 , NR2B , GluR 6 , and KA 2 ) existed in mouse sperm as well as in human sperm . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Preliminary studies with microdialysis and real time PCR analysis support this idea : local ethanol administration in vivo had no effect on glutamate release , but chronic ethanol nearly tripled the expression of NR2B subunits ( the most ethanol sensitive ) in CeA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The interaction of calcium / calmodulin dependent protein kinase 2 ( CaMKII ) with the NR2B subunit of N methyl D aspartate type glutamate receptor is thought to be one of the important events leading to synaptic plasticity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition to changes in NR2B mRNA levels , quantitative polymerase chain reaction analysis revealed that brain derived neurotrophic factor ( BDNF ) and glutamate receptor 5 mRNA levels were also significantly elevated in the DG of Runners , but not in other areas of the hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition , RT PCR results revealed that glutamate transporters ( GLT 1 and EAAC 1 ) and ionotropic glutamate receptors ( NR 1 , NR2B , GluR 6 and KA 2 ) were expressed in mouse testis . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Association of a glutamate ( NMDA ) subunit receptor gene ( GRIN2B ) with obsessive compulsive disorder : a preliminary study . ^^^ We postulated that N methyl d aspartate ( NMDA ) receptors were involved in OCD , and specifically that polymorphisms in the 3 ' untranslated region of GRIN2B ( glutamate receptor , ionotropic , N methyl d aspartate 2B ) were associated with OCD in affected families . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Chronic PCP treated animals showed significant alterations in glutamate receptor subunits , particularly for the NR 1 , NR2B , NR2C , and NR2D components of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We estimated that up to approximately 30 % of NMDARs contributing to EPSCs were activated by glutamate released from multiple synaptic sources ; however , NMDAR mediated synaptic cross talk was undetectable when NR2B subunit containing receptors were blocked ( but could be rescued by blocking glutamate uptake ) . ^^^ We propose that NR2B containing NMDARs can detect glutamate arising from multiple synapses , whereas NR2A containing NMDARs only normally mediate direct synaptic transmission . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2B subunit containing NMDA type glutamate receptors are implicated in the windup of dorsal horn neurons , while their role at the motoneuronal level is controversial . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
However , this decrease was not the same for both subunits , resulting in a decrease ( over 30 % ) in the NR2A : NR2B ratio indicating that the NMDAR had temporarily become more sensitive to glutamate and would remain open longer once activated . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we show that in rats with Freund ' s adjuvant induced inflammation , the increased dorsal horn NR2B tyr P is blocked by group 1 metabotropic glutamate receptor ( mGluR ) antagonists [ 7 ( hydroxyimino ) cyclopropa [ b ] chromen 1a carboxylate ethyl ester ( CPCCOEt ) and 2 methyl 6 ( phenylethynyl ) pyridine ( MPEP ) , by the Src inhibitor CGP 77675 , but not by the MAP kinase inhibitor 2 ' amino 3 ' methoxyflavone . ^^^ Analysis of the calcium pathways shows that the in vivo NR2B tyr P is blocked by an IP 3 receptor antagonist 2 aminoethoxydiphenylborate ( 2APB ) but not by antagonists of ionotropic glutamate receptors and voltage dependent calcium channels , suggesting that the NR2B tyr P is dependent on intracellular calcium release . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
PCR based assays showed that alcoholism was associated with polymorphisms of the dopamine D 2 receptor ( DRD 2 ) TaqI B ( P = . 029 ) and the GABAA beta 2 subunit C1412T ( P = . 012 ) genes , but not with the glutamate receptor subunit gene NMDAR2B ( 366C / G ) , the serotonin transporter gene ( 5HTTL PR ) , the dopamine transporter gene DAT 1 ( SLC6A3 ) , the dopamine D 2 receptor gene DRD 2 TaqI A , or the GABAA alpha 1 ( A15G ) , alpha 6 ( T1519C ) , and gamma 2 ( G3145A ) subunit genes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using the crystal structure of the binding region of the ( RS ) 2 amino 3 ( 3 hydroxy 5 methyl 4 isoxazolyl ) propionic acid ( AMPA ) selective GluR 2 subunit , we identified determinants of ligand selectivity and efficacy within the glutamate binding pocket of the NR2B subunit of the N methyl D aspartate ( NMDA ) receptor by site directed mutagenesis . ^^^ Electrophysiological analyses of mutated NR2B polypeptides revealed drastic effects on the affinity of L glutamate but not of the co agonist glycine . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The figure shows the binding mechanism of glutamate with NR2B subunit of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
For example , blockers selective to the NR2B subunit or agents that block metabotropic glutamate receptors or alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid receptors are currently under investigation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Recordings from outside out patches that contain a single active channel show that NR2A containing receptors have a higher probability of opening at least once in response to a brief synaptic like pulse of glutamate than NR2B containing receptors ( NR2A , 0 . 80 ; NR2B , 0 . 56 ) , a higher peak open probability ( NR2A , 0 . 50 ; NR2B , 0 . 12 ) , and a higher open probability within an activation ( NR2A , 0 . 67 ; NR2B , 0 . 37 ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Analysis of glutamate induced apoptosis of the YAC 128 MSN revealed that ( 1 ) actions of glutamate are mediated by mGluR1 / 5 and NR2B glutamate receptors ; ( 2 ) membrane permeable inositol 1 , 4 , 5 trisphosphate receptor blockers 2 APB and Enoxaparin ( Lovenox ) are neuroprotective ; ( 3 ) apoptosis involves the intrinsic pathway mediated by release of mitochondrial cytochrome c and activation of caspases 9 and 3 ; ( 4 ) apoptosis requires mitochondrial Ca2+ overload and can be prevented by the mitochondrial Ca2+ uniporter blocker Ruthenium 360 ; and ( 5 ) apoptosis involves opening of mitochondrial permeability transition pore ( MPTP ) and can be prevented by MPTP blockers such as bongkrekic acid , Nortriptyline , Desipramine , Trifluoperazine , and Maprotiline . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Because a loss of NR2B subunit inclusion in NR receptors would likely confer increased Ca ( ++ ) conductance and faster deactivation kinetics , the stress induced decrease in NR2B mRNA is consistent with enhanced glutamate signaling in the PVN following chronic stress and , perhaps , increased basal HPA activity and more rapid and / or more robust HPA responses to stress . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To test the predictive value of these models , all four stereoisomers of the antagonist 1 ( phenanthren 2 carbonyl ) piperazine 2 , 3 dicarboxylic acid ( PPDA ) were docked into the NR2B glutamate binding site model . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The increase in glutamate release triggered a long term enhancement of AMPA receptor function that requires activation of postsynaptic NMDA receptors containing NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The magnitude of reversal was greater than other negative modulators of the NMDA receptor studied , such as uncompetitive channel blockers ( e . g . , memantine , dizocilpine , and ketamine ) , a NR2B selective antagonist ( e . g . , ifenprodil ) , and other glutamate antagonists [ e . g . , selfotel , 3 ( 2 carboxypiperazin 4 yl ) propyl 1 phosphonic acid ( CPP ) , D , L ( E ) 2 amino 4 propyl 5 phosphono 3 pentenoic acid ( CGP 39653 ) ] , up to doses that suppressed operant rates of responding . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblotting analysis showed that the NR2A and NR2B subunits of NMDARs were markedly degraded in cultured cortical neurons treated with glutamate , and the degradation of NR 2 subunits was prevented by calpain inhibitors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We show that diminution of network activity in mature neuronal circuit promotes reorganization of neuronal circuits via NR2B subunit containing NMDA type glutamate receptors ( NR2B NMDARs ) , which mediate silent synapse formation . ^^^ The circuit reactivation also caused an increased colocalization of glutamate receptor 1 specfic and synaptic NR2B specific puncta . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The present review describes the role of glutamate in ischemic brain injury with an emphasis on the NR2B containing NMDA receptors . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Synapses at P 2 contained substantial levels of NR 1 and NR2B and most GluR associated proteins , including SAP 102 , SynGAP , the chain of proteins from GluRs / SAP102 through GKAP / Shank / Homer and metabotropic glutamate receptors , and the adhesion factors , cadherin , catenin , neuroligin , and Nr CAM . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Comparative analysis of different competitive antagonists interaction with NR2A and NR2B subunits of N methyl D aspartate ( NMDA ) ionotropic glutamate receptor . ^^^ The antagonist bound conformation of the NR2A and NR2B subunits of N methyl D aspartate ( NMDA ) ionotropic glutamate receptor are modeled using the crystal structure of the DCKA ( 5 , 7 dichloro kynurenic acid ) bound form of the NR 1 subunit ligand binding core ( S1S2 ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have characterized several partial agonists at the NR1 / NR2B receptor and investigated the mechanisms underlying their reduced efficacy by introducing mutations in the glutamate binding site . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The NR2B subunit of N methyl d aspartate ( NMDA ) type glutamate receptor , densin 180 , and alpha actinin bind comparable , approximately stoichiometric amounts of Thr ( 286 ) autophosphorylated CaMKIIalpha , forming a ternary complex ( Robison , A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Either a nonselective NMDA receptor antagonist ( 5R , 10S ) ( + ) 5 methyl 10 , 11 dihydro 5H dibenzo [ a , d ] cyclohepten 5 , 10 imine hydrogen maleate ( MK 801 ) or selective NR2B subunit containing NMDA receptor antagonists ifenprodil and ( R , S ) alpha ( 4 hydroxyphenyl ) beta methyl 4 ( phenylmethyl ) 1 piperid inepropanol ( Ro 25 6981 ) significantly inhibited the glutamate evoked Ca ( 2+ ) influx into neurons , but not into astrocytes . ^^^ These results suggest that NR2B subunit containing NMDA receptor plays a critical role in not only glutamate evoked Ca ( 2+ ) influx into neurons , but also glutamate induced astrocytic activation . ^^^ Thus , glutamate mediated pathway via NR2B subunit containing NMDA receptor may , at least in part , contribute to neuron to astrocyte signaling . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Traxoprodil ( CP 101 , 606 ) is a novel and potent glutamate receptor antagonist that is highly selective for the NR2B subunit of the NMDA receptor ; it has been shown to be neuroprotective in animal models of brain injury and ischemia . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Sections were processed and analyzed for [ 3H ] glutamate binding to NMDA receptors using receptor autoradiography and for mRNA for the zeta 1 ( NR 1 ) , epsilon 1 ( NR2A ) , and epsilon 2 ( NR2B ) subunits of the NMDA receptor using in situ hybridization . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tonic facilitation of glutamate release by presynaptic NR2B containing NMDA receptors is increased in the entorhinal cortex of chronically epileptic rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
GT 1 7 neurons express mRNAs for various mouse NMDA receptor subunits ( zeta , 1 , epsilon 3 , epsilon 4 , and epsilon 2 , corresponding to the NR 1 , NR2C , NR2D , and NR2B rat subunits ) and increase their spontaneous release of GnRH when incubated in the presence of exogenous glutamate or NMDA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The glutamate neurotransmitter system has been implicated in psychosis and schizophrenia , and so we looked for genetic association and measured gene expression in human DNA and brain samples , respectively , of the GRIN2B gene that codes for the NR2B protein . ^^^ The NR2B protein is a critical structural and functional subunit of the NMDA glutamate receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
However , activity dependent phosphorylation of substrates at synapses was highly selective in that the glutamate receptor subunits NR2B and GluR 1 were poorly phosphorylated whereas PSD 95 and Stargazin , proteins implicated in the scaffolding and trafficking of AMPA receptors , were robustly phosphorylated . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The expression of several other synaptic proteins ( NR2B , glutamate receptors 2 / 3 , N ethylmaleimide sensitive factor ) was not affected by long term potentiation induction . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N Methyl D aspartate receptor subunit genes ( gene symbol , GRIN ) GRIN 1 , GRIN2A , GRIN2B , GRIN2C , and GRIN2D have been assigned to chromosomes ( Chr ) 3 , 10 , 4 , 10 , and 1 , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The murine chromosomal locations of the five NMDA receptor channel subunits , the epsilon 1 ( Grin2a ) , epsilon 2 ( Grin2b ) , epsilon 3 ( Grin2c ) , epsilon 4 ( Grin2d ) and zeta 1 ( Grinl ) subunits , were determined using an interspecific backcross mapping panel derived from crosses of [ ( C57BL / 6JxM . spretus ) F1xC57BL / 6J ] mice . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Glutamate receptor , ionotropic , N methyl D aspartate 2A ( GRIN2A ) gene as a positional candidate for attention deficit / hyperactivity disorder in the 16p13 region . ^^^ The glutamate receptor , ionotropic , N methyl D aspartate 2A ( GRIN2A ) gene that encodes the 2A subunit of the NMDA receptor , resides in this region and a recent study has reported an association between this gene and ADHD . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The GRIN2A ( glutamate receptor , ionotropic , N methyl D aspartate 2A ) gene that encodes the N methyl D aspartate receptor subunit 2A ( NMDA2A ) maps to this region of linkage . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the studied cohort of 167 HD patients , the repeat range from 41 to 45 CAG units accounted for 30 . 8 % of the variance in AO ; 12 . 3 % additional variance could be attributed to GRIN2B genotype variation and 4 . 5 % to GRIN2A genotype variation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
There is also growing evidence for the role of DRD 1 , NMDA receptor genes ( GRIN 1 , GRIN2A , GRIN2B ) , brain derived neurotrophic factor ( BDNF ) , and dopamine transporter ( SLC6A3 ) in both schizophrenia and bipolar disorder . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Analysis of correlation between serum D serine levels and functional promoter polymorphisms of GRIN2A and GRIN2B genes . ^^^ Two polymorphisms are known to regulate the expression of NMDA receptor subunit genes : ( GT ) ( n ) ( rs 3219790 ) in the promoter region of the NR2A subunit gene ( GRIN2A ) and 200T > G ( rs 1019385 ) in the NR2B gene ( GRIN2B ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Molecular cloning identified three complementary DNA species of rat brain , encoding NMDA receptor subunits NMDAR2A ( NR2A ) , NR2B , and NR2C , which are 55 to 70 % identical in sequence . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Optimal expression of cloned NMDAR1 / NMDAR2A heteromeric glutamate receptors : a biochemical characterization . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDAR 2 subunits also exhibit differential expression : NMDAR2B is abundant in the striatum , but NMDAR2A is present within the striatum only at low levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast , mRNA for NMDAR2A , NMDAR2B , NMDAR2C and GluR 3 was not detected in the SCN . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
An antisense ODN to NMDAR2A was also effective in eliciting amphetamine inducible rotation , although the magnitude of the effect was less than that seen with NMDAR 1 , whereas ODNs targeted to NMDAR2B , NMDAR2C and an NMDAR 1 sense strand ODN had no effect on behavior . ^^^ In situ hybridization demonstrated that injection of the NMDAR 1 , NMDAR2A or NMDAR2B antisense ODNs produced specific reductions in target mRNA signal intensity in the injected striatum . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The signals for N methyl D aspartate ( NMDA ) R 1 and NMDAR2B mRNAs were high in most neurons ; however , NMDAR2A and NMDAR2C mRNAs gave low or undetectable signals . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Human cDNAs encoding N methyl D aspartate receptor type ( NMDAR ) 1A , NMDAR2A and NMDAR2B subunits were cloned and receptors encoded by these cDNAs were functionally expressed by injection of the respective mRNAs in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Film autoradiograms demonstrated a distinctive laminar distribution of hybridization signals for each of the probes recognizing NMDAR 1 , NMDAR2A , and NMDAR2B messenger RNA ; hybridization with probes for NMDAR2C and NMDAR2D resulted in scattered signals without laminar organization . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Dual in situ hybridization using digoxigenin labeled GnRH cRNA probes and 35S labeled glutamate receptor subunit probes , followed by autoradiography and image analysis were used to measure the KA 2 or NMDAR2A mRNA content in GnRH neurons in 20 to 50 day old female rats which were sacrificed at 08 . 00 or 17 . 00 h . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Acute heat stress induces edema and nitric oxide synthase upregulation and down regulates mRNA levels of the NMDAR 1 , NMDAR2A and NMDAR2B subunits in the rat hippocampus . ^^^ On the other hand NMDA receptor messenger RNA encoding NMDAR 1 , NMDAR2A and NMDAR2B subunits showed a marked downregulation in the hippocampus of heat stressed rats compared to the controls . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Gene expression studies of mRNAs encoding the NMDA receptor subunits NMDAR 1 , NMDAR2A , NMDAR2B , NMDAR2C , and NMDAR2D following long term treatment with cis and trans flupenthixol as a model for understanding the mode of action of schizophrenia drug treatment . ^^^ NMDAR2A , 2B and 2C receptor subunits showed a significantly decreased expression from 12 to 24 weeks but after 2 weeks NMDAR2B , 2C , 2D expression was increased in several brain regions . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Frozen sections and serial plastic sections of tissue were incubated with antibodies to NMDAR 1 ( NR 1 ) , NMDAR2A ( NR2A ) , NMDAR2A / B ( NR2A / B ) , and NMDAR2B ( NR2B ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunocytochemical localization of metabotropic glutamate receptors ( mGluRs ) and ionotropic glutamate receptors ( NMDA type : NMDAR 1 and NMDAR2A C ; AMPA type : GluR 1 4 ) was performed on sections of rat dorsal horn . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The effects of prenatal ethanol exposure on the NMDAR 1 protein expression ( postnatal days 1 and 7 ) and on the developmental profile of the NMDAR2A and NMDAR2B subunits in rat forebrain and hippocampus were investigated . ^^^ A semiquantitative immunoblot procedure was used with antibodies raised against the NMDAR 1 , NMDAR2A , and the NMDAR2B subunits to assess the NMDA subunit protein expression in the samples . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We used quantitative double label in situ hybridization to examine in rat brain the expression of NMDAR 1 , NMDAR2A , NMDAR2B , and NMDAR2C mRNA in six neurochemically defined populations of striatal neurons : preproenkephalin ( ENK ) and preprotachykinin ( SP ) expressing projection neurons , and somatostatin ( SOM ) , glutamic acid decarboxylase 67 ( GAD 67 ) , parvalbumin ( PARV ) , and choline acetyltransferase ( ChAT ) expressing interneurons . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
However , analysis of NMDAR2A and NMDAR2B protein expression in hippocampus indicated a significant decrease between 21 and 30 months of age and administration of IGF 1 increased these receptor subtypes . ^^^ In cortex , NMDAR2A and NMDAR2B protein expression were not influenced by age or IGF 1 treatment , although NMDAR2C protein expression decreased with age and this decline was not ameliorated by IGF 1 administration . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N methyl D aspartate ( NMDA ) receptor 1 ( NMDAR 1 ) , NMDAR2A , and NMDAR2B mRNAs were expressed highly throughout the PVN and its perinuclear region as well as in the subparaventricular zone . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To gain a better understanding about the precise sites of action of glutamate and the subunit compositions of the receptors involved in the glutamatergic neurotransmission in the hypothalamus and septum , in situ hybridization was used with 35S labeled cRNA probes for the different ionotropic receptor subunits , including glutamate receptor subunits 1 4 ( GluR 1 GluR4 ) , kainate 2 , GluR 5 GluR7 , N methyl D aspartate ( NMDA ) receptor 1 ( NMDAR 1 ) , and NMDAR2A NMDAR2D . ^^^ The results showed that subunits of alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate preferring , kainate preferring , and NMDA preferring receptor subunits are distributed widely but heterogeneously and that the GluR 1 , GluR 2 , kainate 2 , NMDAR 1 , NMDAR2A , and NMDAR2B subunits are the most abundant in the hypothalamus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blot analysis also failed to reveal any changes in NMDAR 1 , NMDAR2A , or NMDAR2B receptor levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This approach has allowed us to assess the responses of individual NTS neurones in vivo to ionotropic glutamate receptor agonists and antagonists and then , to determine whether the neurones expressed the glutamate receptor subunits , GLUR 2 , 3 and NMDAR2a , b . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The mRNA expression of the subunits of rat hippocampus NMDAR NMDAR 1 , NMDAR2A , NMDAR2B , NMDAR2D was determined with RT PCR technique in C group and at 0 . 5 , 1 , 2 and 4 postburn hours ( PBHs ) in S group . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
An antibody that recognizes both mammalian NMDAR2A and NMDAR2B subunits equally was used . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In bone sections from young adult rats , osteoclasts expressed numerous GluR subunits including AMPA ( GluR2 / 3 and GluR 4 ) , kainic acid ( GluR 567 ) and NMDA ( NMDAR2A , NMDAR2B and NMDAR2C ) receptor subtypes . ^^^ Bone lining cells demonstrated immunoexpression for NMDAR2A , NMDAR2B , NMDAR2C , GluR 567 , GluR 23 , GluR 2 and GluR 4 subunits . ^^^ Mechanically loaded limbs revealed a load induced loss of immunoexpression for GluR2 / 3 , GluR 4 , GluR 567 and NMDAR2A on osteoclasts and NMDAR2A , NMDAR2B , GluR2 / 3 and GluR 4 on bone lining cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Moreover , in the CA 3 area we have detected a concomitant increase ( 50 % above controls ) in the level of NR2B subunit messenger RNA , while the expression of NR2A subunit gene did not change after stress . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptor subunits epsilon 1 ( NR2A ) and epsilon 2 ( NR2B ) are substrates for Fyn in the postsynaptic density fraction isolated from the rat brain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2B and NR2D mRNAs occur prenatally , whereas NR2A and NR2C mRNAs are first detected near birth . ^^^ In the adult hippocampus , NR2A and NR2B mRNAs are prominent in CA 1 and CA 3 pyramidal cells , but NR2C and NR2D mRNAs occur in different subsets of interneurons . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We studied heteromeric assemblies ( NR1b / NR2 ) of the NMDA receptor , expressed in oocytes , to address three questions regarding this inhibition , and discovered the following : ( 1 ) The inhibition was nearly equivalent when ethanol was coapplied with the agonist , and when ethanol was introduced after steady state current was established , suggesting that ethanol does not act by interfering with the activation process of the NMDA receptor . ( 2 ) The degree of inhibition was controlled by the NR 2 subunit , with both NR2A and NR2B significantly more sensitive to ethanol than NR2C and NR2D . ( 3 ) Manipulation of the NMDA receptor with a number of agents that normally modulate it did not alter the degree of inhibition produced by ethanol . ^^^ The presence of Mg2+ ( 3 and 12 . 5 microM ) , Zn2+ ( 1 and 10 microM ) , or the glycine antagonist 7 chlorokynurenic acid ( 1 . 25 or 5 microM ) , did not alter the ethanol sensitivity of heteromeric ( NR1b / NR2A , NR1b / NR2B , NR1b / NR2C ) NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoaffinity chromatography of detergent extracts of rat synaptic plasma membranes on anti phosphotyrosine antibody agarose showed that the NR2A and NR2B subunits but not the NR 1 subunit are tyrosine phosphorylated . ^^^ Conversely , immunoprecipitation of the NR 1 , NR2A , and NR2B subunits with subunit specific antibodies followed by immunoblotting with anti phosphotyrosine antibodies confirmed that the NR2A and NR2B subunits but not the NR 1 subunit were phosphorylated on tyrosine residues . ^^^ It was estimated that 2 . 1 + / 1 . 3 % of the NR2A subunits and 3 . 6 + / 2 . 4 % of the NR2B subunits were tyrosine phosphorylated in vivo . ^^^ In addition , endogenous protein tyrosine kinases in synaptic plasma membranes phosphorylated the NR2A subunit in vitro , increasing its phosphorylation 6 8 fold but did not phosphorylate NR 1 or NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast to the marked influence of chronic NMDA exposure on the cellular content of the NMDA receptor subunit proteins , mRNA levels of the different subunits ( NR 1 , NR2A , NR2B and NR2C ) were not significantly affected . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the fascia dentata region , a minor increase of NR2A and NR2B transcripts was found at all stages of kindling acquisition . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The glutamate receptor channels of the N methyl D aspartate ( NMDA ) subtype are composed of different subunits named NR 1 and NR2A D . ^^^ Consistent with this observation , their responses to NMDA were only weakly reduced by 3 microM ifenprodil , a non competitive antagonist which discriminates between NR2A and NR2B subunits in expression systems . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In heteromeric NR1A / epsilon 4 receptors the epsilon 4 subunit may alter properties associated with the NR1A subunit , as has been previously suggested for NR2A , NR2B , and NR2C subunits in NR1A / NR2 receptors . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Among the NMDA receptor subunits , NR 1 was most highly expressed in the SCN followed in order of abundance by NR2B , NR2A , NR2C and NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Characterization of NMDA receptor subunit specific antibodies : distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum . ^^^ Selective antisera for NMDA receptor subunits NR2A and NR2B have been developed . ^^^ It was found that although the distribution of the NR2A subunit corresponds well with radioligand binding , the distribution of the NR2B subunit does not . ^^^ The ontogenic profiles of NR2A and NR2B subunits in the rat cerebellum were also determined . ^^^ Just following birth [ postnatal day ( P ) 2 ] NR2A subunits are undetectable , whereas NR2B subunits are expressed at amounts easily measurable . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Five N methyl D aspartate ( NMDA ) receptor subunits have been identified thus far : NR 1 , NR2A , NR2B , NR2C , and NR2D . ^^^ In the external granular layer , hybridization signals of mRNAs for NR 1 , NR2A , NR2B , and NR2C appeared by postnatal day 3 , but no NR2D mRNA was expressed at any developmental stage examined . ^^^ In the cerebellar nuclei , mRNAs for NR 1 , NR2A , NR2B , and NR2D were more or less expressed on postnatal day 0 , while expression signals for the NR2C mRNA were first detected in postnatal day 14 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
On the basis of the Mg2+ sensitivity and expression patterns , we have proposed that the epsilon 1 ( NR2A ) and epsilon 2 ( NR2B ) subunits play a role in synaptic plasticity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In cortex , the expression of NR2A and NR2B mRNAs corresponds to the previously described developmental profile of NMDA receptor subtypes having low and high affinities for ifenprodil , i . e . , a delayed expression of NR2A correlating with the late expression of low affinity ifenprodil sites . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
However , high K+ and NMDA treatment resulted in a greater increase of NR2A mRNA levels and a retardation in the developmental changes in the relative amounts of NR2B and NR2C mRNAs . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Fourteen glutamate receptor subunits were studied : GluR 1 4 ( including flip and flop variants ) , GluR 5 7 , KA 1 & 2 , NR 1 , and NR2A D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The pharmacology of native NMDA receptors was compared to that of heteromeric NMDA receptors expressed in Xenopus oocytes ( NR1 / NR2A , NR1 / NR2B , NR1 / NR2C ) . ^^^ The oocyte expressed NR1 / NR2A receptor displayed a higher affinity for antagonists and a slightly lower affinity for agonists than the NR1 / NR2B receptor . ^^^ NMDA receptors in the lateral thalamus ( with a high density of NR2A subunit mRNA ) displayed higher affinity for antagonists and a lower affinity for agonists than did NMDA receptors of the medial striatum ( a region rich in NR2B mRNA ) . ^^^ Relative to the NR1 / NR2A and NR1 / NR2B receptors , oocyte expressed NR1 / NR2C receptors had a lower affinity specifically for both D 3 ( 2 carboxypiperazin 4 yl ) 1 propenyl 1 phosphonic acid ( D CPPene ) and homoquinolinate ( HQ ) . ^^^ This pattern was identical to that observed for cerebellar ( NR2C containing ) versus forebrain ( NR2A and NR2B containing ) NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This antibody ( NR2A / B ) did not cross react with extracts from transfected cells expressing other glutamate receptor subunits , nor did it label non neuronal tissues . ^^^ The NMDA receptor subunits NR2A and NR2B show histological and ultrastructural localization patterns similar to those of NR 1 . ^^^ In analysis of transfected cell membranes , this antibody recognizes NR2A and NR2B , and to a slight extent , NR2C and NR2D . ^^^ In Western blots of rat brain , the antibody labeled a single band that comigrated with NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The expression patterns of nine genes encoding the N methyl D aspartate ( NMDA ) receptor subunits NR 1 and NR2A , NR2B , NR2C and NR2D , and the high affinity kainate receptor subunits KA 1 , KA 2 , GluR 6 and GluR 7 , were studied in the adult rat retina by in situ hybridization . ^^^ Hybridization with [ 35S ] dATP labelled oligonucleotide probes revealed the expression of four of the NMDA receptor subunits ( NR 1 , NR2A , NR2B and NR2C ) and three of the high affinity kainate receptor subunits ( KA 2 , GluR 6 and GluR 7 ) in the retina . ^^^ In the ganglion cell layer , virtually every ganglion cell or displaced amacrine cell expressed the receptor subunits NR 1 , NR2A , NR2B , NR2C , KA 2 and GluR 7 . ^^^ The GluR 6 , NR2A , NR2B and NR2C subunits were expressed by subsets of amacrine cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 ( 011 ) , the most prominent NR 1 splice variant in rat forebrain , and NR 1 ( 100 ) , prominent in midbrain , were expressed in Xenopus oocytes singly and in combination with NR2A , NR2B , and NR2C subunits . ^^^ As for NR 1 ( 100 ) homomers , NR 1 ( 100 ) / NR2B and NR 1 ( 100 ) / NR2A receptors exhibited spermine potentiation only by increasing the glycine affinity . ^^^ Thus , the NR2B subunit `` permits ' ' both forms of spermine potentiation , the NR2A subunit permits spermine potentiation only by increasing the glycine affinity , and th NR2C subunit permits neither form of potentiation . ^^^ At early postnatal ages , NR2B subunit mRNA was more highly expressed than NR2A and NR2C mRNAs in hippocampus , neocortex , and caudate putamen . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
A concomitant reduction in levels of NR2B but not NR2A occurred in NR 1 / mice , demonstrating that there is an interdependence of subunit expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
As a model of complex partial seizures , rapid hippocampal kindling was chosen to investigate changes in the expression of messenger RNAs encoding the N methyl D aspartate receptor subunits NR 1 , NR2A and NR2B both during and in the period immediately following the induction of the kindled state . ^^^ In partially kindled animals ( 10 stimulations ) , while the NR 1 subunit messenger RNA remained unaltered after a period of 2 h , the NR2A and NR2B subunit messenger RNAs were bilaterally reduced in dentate gyrus granule cells by around 50 % below control values . ^^^ At the same time point , NR2A and NR2B transcript levels were transiently increased by 102 % and 46 % above control values , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
By co immunoprecipitation with subunit specific antibodies , we present here direct evidence that NMDA receptors exist in rat neocortex as heteromeric complexes of considerable heterogeneity , some containing both NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using expression cloning , and more recently using polymerase chain reaction cloning approaches , a family of rat N methyl D aspartate ( NMDA ) receptor subunit cDNAs has been described ( NR 1 , NR2A , NR2B , NR2C , and NR2D ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At this developmental age , levels of NR 1 , NR2A , NR2B , and NR2C mRNA were low or undetectable . ^^^ The level of NR2B mRNA was approximately 4 fold higher than that of NR2A mRNA in 21 day cultures . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A , NR2B , and GluR 6 mRNAs were undetectable . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Based on its molecular size the photolabeled protein is likely to correspond to the NR2A and / or NR2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
When the NR 1 subunit was coexpressed with either the NR2A , NR2B , or NR2C subunit , only the combination of NR1 / NR2A was photoreactive . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA treatment also elicited a decrease in NR 1 , NR2A , and NR2B subunit messenger RNA ( mRNA ) levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Reverse transcription PCR assays were used to measure levels of NMDA receptor ( NR ) subunit mRNAs encoding splice variants of NR 1 ( NR1a , exon 5 ; NR1b , +exon 5 ) and the major NR 2 subunits ( NR2A , NR2B , and NR2C ) in dissociated cerebellar granule cell cultures . ^^^ In situ granule cell maturation is associated with downregulation of NR2B and increases both of NR2A and NR2C and in the ratio of NR1b / NR1a mRNAs . ^^^ In culture , 25 mM KCl or NMDA rapidly induced NR2A and downregulated NR2B , followed by gradual induction of NR2C . ^^^ In 5 mM KCl , a similar , rapid increase in NR2A was observed , but disappearance of NR2B occurred over a longer time course . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N methyl D aspartate receptor proteins NR2A and NR2B are differentially distributed in the developing rat central nervous system as revealed by subunit specific antibodies . ^^^ We have identified the regional distributions and developmental expression of NMDA receptor proteins NR2A and NR2B in rat CNS , using two subunit specific affinity purified polyclonal antibodies that recognize NR2A and NR2B . ^^^ In western blots of cells transfected with NR2A or NR2B cDNAs , and of brain homogenates , each antibody detects a single predominant 172 kDa protein corresponding to its homologous subunit . ^^^ In adult rat CNS , NR2A and NR2B are enriched in cortex and hippocampus but are present in other forebrain regions . ^^^ In hindbrain , NR2A is present at low levels but NR2B is barely detectable . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
With use of the yeast two hybrid system to screen a rat brain cDNA library and by in vitro binding assays , we have identified an interaction between NMDA receptor subunits 2A and 2B ( NR2A and NR2B ) and three distinct members of the PSD 95 / SAP90 family of membrane associated putative guanylate kinases . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the cerebral cortex of both schizophrenics and controls , mRNAs for NR 1 , NR2A , NR2B , and NR2D subunits were preferentially expressed in layers II / III , Va , and VIa , with much higher levels in the prefrontal than in the parieto temporal cortex . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ifenprodil is an atypical noncompetitive modulator of the N methyl D aspartate ( NMDA ) receptor ( NR ) which demonstrates a 140 fold preference for NR2B over NR2A subunits , although the molecular basis for this subunit specificity is unknown . ^^^ We have made chimeric receptors by fusing the murine forms of NR2A ( epsilon 1 ) and NR2B ( epsilon 2 ) to localize the high affinity determinants of ifenprodil inhibition on the 2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The N methyl D aspartate ( NMDA ) subtype of ionotropic glutamate receptor is assembled from one NR 1 subunit , expressed in eight splice variants , and four NR 2 subunits ( NR2A D ) . ^^^ In the present study we investigated the relationship between NR 1 splice variants and NR 2 subunits in rat brain using a series of antibodies selective for the four NR 1 cassettes , which vary in the NR 1 splice variants , and for NR2A and NR2B . ^^^ Immunoprecipitation analyses using antibodies selective for NR2A and NR2B showed no preferential assembly between NR 2 subunits and NR 1 splice variants . ^^^ There was little co immunoprecipitation of NR2A and NR2B , suggesting that most NMDA receptor complexes contain only one of these subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A immunoreactivity was found in almost all regions of the brain , whereas NR2B staining was restricted to forebrain , and NR2D immunoreactivity to diencephalic , mesencephalic and brain stem structures . ^^^ Thus , NMDA receptors containing the NR2A subunit are likely to represent a receptor subtype predominant throughout the brain , while those containing the NR2B , NR2C or NR2D subunit represent more region specific receptor subtypes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we show that two of these subunits , NR2B and NR2A , are phosphorylated on tyrosine residues in vivo , in rat striatum , where NR2B is by far the most prominent tyrosine phosphorylated protein . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptors from 1 day old rat cerebral cortex were more sensitive to activation by glycine than were receptors from adult rat cerebral cortex . 7 Chlorokynurenate and CGP 39653 were more potent inhibitors of responses seen with heteromeric NR1 / NR2A receptors than with NR1 / NR2B receptors . ^^^ Conversely , heteromeric NR1 / NR2B receptors were more sensitive to activation by glycine than were NR1 / NR2A receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The divalent ion permeability of Insert 1 containing and Insert 1 lacking receptor channels expressed alone , as well as in heteromeric assemblies with NR2A and NR2B , was similar for all combinations tested . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The AMPA receptor subunits GluR A , GluR B and GluR C , and the NMDA receptor subunits NR2A and NR2B were found to be abundantly expressed in all CA 1 pyramidal neurones tested . 5 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Distribution of the N methyl D aspartate glutamate receptor subunit NR2A in control and amyotrophic lateral sclerosis spinal cord . ^^^ The aim of this study was to examine the distribution of the N methyl D aspartate ( NMDA ) glutamate receptor modulatory subunit NR2A , in control human spinal cord and to examine in parallel the expression of the mRNA in amyotrophic lateral sclerosis ( ALS ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Mutations at equivalent positions in NR2A ( E352Q ) or NR2B ( E353Q ) had no effect on sensitivity to spermine , pH , or ifenprodil . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 2 subunits containing mutations at NR2A ( D 731 ) and NR2B ( D 732 ) , which correspond to NR 1 ( D 732 ) , did not produce functional receptors when coexpressed with NR 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The relative distribution profile of NMDA receptor subunits in the cerebral cortex was NR2B > NR 1 > NR2A > NR2C and in the cerebellum NR2C = NR 1 > NR2A = NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The expression of NMDA receptor genes ( NR 1 , NR2A B ) was examined by in situ hybridization using oligonucleotide probes , and binding to NMDA receptors assessed using L [ 3H ] glutamate . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We found that more than 70 % of CRF positive neurons contain the NMDA receptor subunit NR 1 mRNA whereas NR2A and NR2B subunit mRNAs were not detectable in CRF cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Various NMDA receptor antagonists , in a dose dependent manner , prevented a loss of luciferase activity 24 to 48 hr post transfection of either the NR1 / NR2A or NR1 / NR2B subunit receptor configurations , likely correlating to the time required to express functionally these receptors . ^^^ The novel ifenprodil analog ( 1S , 2S ) 1 ( 4 hydroxyphenyl ) 2 ( 4 hydroxy 4 phenylpiperidino ) 1 propanol ( CP 101 , 606 27 ) protected cells expressing NR1 / NR2B , but not those cells expressing either NR1 / NR2A or , putatively , NR1 / NR2A / NR2B . ^^^ Decreased cytotoxicity was observed when a mutated NR 1 subunit ( N616R ) with reduced Ca++ permeability was used in coexpression studies with NR2A or NR2B . ^^^ In contrast to our results with NR1 / NR2A or NR1 / NR2B , cells expressing NR1 / NR2C did not perish . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Similarly , the NR2D subunit is selectively and quantitatively immunoprecipitated , whereas the NR 1 , NR2A , or NR2B subunit is not . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We show that serine 1303 in the full length NR2B and / or the cognate site in NR2A is a major site of phosphorylation of the receptor both in the postsynaptic density fraction and in living hippocampal neurons . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To further characterize this inhibition , we used electrical recordings to assay the effects of haloperidol on four diheteromeric subunit combinations of cloned rat NMDA receptors expressed in Xenopus laevis oocytes : NR1A coexpressed with NR2A , NR2B , NR2C , or NR2D . ^^^ This change in pharmacology coincides with a developmental switch in subunit expression ; from NR 1 expressed with NR2B to NR 1 coexpressed with NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptor heterogeneity during postnatal development of the rat brain : differential expression of the NR2A , NR2B , and NR2C subunit proteins . ^^^ At birth , the NR2B subunit was detected almost ubiquitously , the NR2A subunit staining was faint and restricted to the hippocampus , cerebral cortex , and striatum , and no NR2C subunit immunoreactivity was detected . ^^^ During the first 3 postnatal weeks , the NR2B subunit became confined to forebrain structures , whereas the NR2A immunoreactivity became abundantly expressed throughout the brain . ^^^ After 3 weeks , NR2A and NR2B immunoreactivity decreased to adult levels , whereas NR2C immunoreactivity remained unchanged . ^^^ Our results reveal a progressive increase in the heterogeneity of NMDA receptors due to the comparably late onset of NR2A and NR2C subunit expression and by the area specific rearrangement of NR2B subunit expression following birth . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoaffinity purification of NMDA receptors from forebrain by anti NR 1 911 920 antibody affinity chromatography from 1 % Triton 10 100 / 1 M NaCl solubilised extracts yielded purification of the NR 1 Mr 120 , 000 immunoreactive species , but no detectable NR2A or NR2B immunoreactivity . ^^^ Immunoprecipitation of NMDA receptors from Triton 10 100 / 1 M NaCl extracts with anti NR 1 911 920 antibodies also resulted in precipitation of NR 1 subunits , but with no detectable NR2A or NR2B subunits . ^^^ In contrast , by immunoprecipitation with anti NR 1 17 35 antibodies , which recognise all forms of NR 1 , NR 1 , NR2A , and NR2B immunoreactivities were detected in the immune pellets . ^^^ Similarly , a co association of NR 1 , NR2A , and NR2B subunits was demonstrated following extraction of forebrain membranes with 1 % sodium deoxycholate ( pH 9 ) and purification by anti NR 1 911 920 antibody affinity chromatography . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The majority of N methyl D aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits ( NR1 / NR2A / NR2B ) . ^^^ With the goal to determine quantitatively the subunit composition of cortical NMDA receptors , we used the monoclonal antibody to NR 1 and polyclonal antibodies against the NR2A and NR2B subunits to perform immunoprecipitations of receptor subunits from solubilized adult rat cortical membranes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using immunohistochemical double labeling with a specific antibody recognizing both NR2A and NR2B subunits , we studied the cellular distribution of the NMDA receptor subunit NR2A / 2B on all major known striatal neuron types . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Glutamate activated currents ( evoked every 20 s for up to 20 min ) were increased by src and fyn kinases without affecting the desensitization and deactivation kinetics in NR 1 NR2A but the kinases had no effects in NR 1 NR2B , NR 1 NR2C and NR 1 NR2D receptor channels , suggesting that a phosphorylation site in NR2A is targeted . 4 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The pharmacology of recombinant NR1a / NR2D NMDA receptors expressed in Xenopus oocytes was examined and compared to the pharmacology of NR1a / NR2A , NR1a / NR2B and NR1a / NR2C heteromers . ^^^ These findings parallel observations obtained for native NMDA receptors in the medial thalamus ( presumed to contain NR2D subunits ) and forebrain ( presumed to contain NR2A and NR2B subunits ) . ^^^ Thus , only compounds that discriminate between either NR2A or NR2B containing heteromers and NR2D containing heteromers also discriminate between forebrain and medial thalamic NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
When tested for NR2A C , all but one neurone were shown to express mRNA for NR2B . ^^^ The NR2B subunit mRNA was usually detected together with NR2A or NR2C . mRNA for NR2D was detected in most neurones from a separate batch of cells tested only for this subunit . 3 . ^^^ The NR 2 subunit mRNA detected and the single channel conductance levels observed suggest that , in addition to NR2B , which is present in nearly all cells , NR2A , NR2C and NR2D are also expressed . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To determine if these differences might be due to NMDA receptor composition , we compared the expression of NR 1 , NR2A , NR2B , NR2C and NR2D subunit mRNAs in immunocytochemically identified vasopressin and oxytocin neuroendocrine cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Developmental expression , subcellular localization , and tyrosine phosphorylation of NR2A and NR2B in the rat brain . ^^^ We carried out quantitative analyses of the developmental expression , subcellular localization of the N methyl D aspartate receptor subunit 2A ( NR2A ) and 2B ( NR2B ) , and tyrosine phosphorylation of NR2B . ^^^ There were 2 . 26 + / 0 . 40 fold more NR2B than NR2A proteins in the forebrain PSD fractions , and NR2A and NR2B were enriched 2 . 75 + / 0 . 35 and 4 . 65 + / 0 . 25 fold , respectively , in the synaptosome , and 13 . 75 + / 0 . 80 and 16 . 04 + / 0 . 25 fold , respectively , in the PSD fraction from brain homogenate . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Double immunoprecipitation experiments show that all three NMDA receptor subunits that are prevalent in the cortical structures , NR 1 , NR2A , and NR2B , are substrates for PKA as well as PKC . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Memantine blocked and could be trapped by recombinant NMDA receptors composed of NR 1 and either NR2A or NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To evaluate whether changes in the expression of N methyl D aspartate receptor subunits might contribute to these effects , the expression of NR 1 , NR2A , NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analysis of expression of NR 1 , NR2A , and NR2B receptor subunits showed no difference between control and chronic ethanol treated cultures . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Effects of an anabolic androgenic steroid on the regulation of the NMDA receptor NR 1 , NR2A and NR2B subunit mRNAs in brain regions of the male rat . ^^^ The expression of the N methyl D aspartate ( NMDA ) receptor subunits NR 1 , NR2A and NR2B mRNAs was examined in discrete areas of the male rat brain ( including hippocampus , hypothalamus , nucleus accumbens and cortex ) following 14 days daily intramuscular injections of high doses ( 5 and 15 mg / kg ) of an anabolic androgenic steroid ( AAS ) ( nandrolone decanoate ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We used in situ hybridization histochemistry to measure the messenger RNA levels for the NR 1 , NR2A and NR2B subunits of the N methyl D aspartate receptor and [ 3H ] dizocilpine maleate ( a non competitive antagonist ) binding to measure N methyl D aspartate receptor density . ^^^ In the intact animal , corticosterone increased messenger RNA levels for NR2A and NR2B but not NR 1 subunits of the N methyl D aspartate receptor in all regions of the hippocampus . ^^^ We used ifenprodil inhibition of [ 3H ] dizocilpine maleate binding , which has been used to distinguish NR2A from NR2B containing receptors , to determine whether corticosterone altered the proportion of high and low affinity sites for ifenprodil in parallel with the changes in subunit messenger RNA levels . ^^^ These data suggest that the effects of corticosterone on hippocampal function are mediated , in part , by parallel increases in NR2A and NR2B subunit levels and the number of receptor channel binding sites . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The model is constructed by co expressing glycine / Na / Cl cotransporters ( GLYT1b ) and NMDA receptors ( NMDARs ) ( composed of the subunits NR 1 and NR2A or NR2B ) in Xenopus oocytes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To further investigate the modulatory effects of zinc on NMDA receptors of different subunit compositions , we coexpressed the recombinant subunit NR 1 with NR2A and / or NR2B in HEK 293 cells . ^^^ Cells cotransfected with NR 1 , NR2A , and NR2B showed zinc sensitivity intermediate between that characteristic of NR1 / NR2A and that of NR1 / NR2B . ^^^ Furthermore , zinc accelerated the macroscopic desensitization of both NR1 / NR2A and NR1 / NR2B in a dose dependent manner , apparently independently of glycine sensitive desensitization and Ca 2 ( + ) dependent inactivation ; maximal effects were to decrease desensitization time constants for NR1 / NR2A by approximately 75 % and for NR1 / NR2B by approximately 90 % . ^^^ Differential modulation of NR1 / NR2A and NR1 / NR2B currents by zinc may play a role in regulating NMDA receptor induced synaptic plasticity and neurotoxicity . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
For example , while NR2A is important for Ca dependent inactivation of NMDA receptors , studies in several systems suggest that the other major NR 2 subunit in hippocampal neurons , NR2B , predominates at critical times during synapse formation . ^^^ The essential role of NR 1 and NR2B in development is graphically demonstrated by the neonatal death of transgenic mice lacking either of these two subunits ( Forrest et al . , 1994 ; Kutsuwada et al . , 1996 ) whereas NR2A and NR2C deficient mice are less severely affected ( Sakimura et al . , 1995 ; Ebralidze et al . , 1996 ) . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The comparison of NR1a NR2A and NR1a NR2B receptors shows that the voltage dependent inhibition is similar in both types of receptors but that the voltage independent inhibition occurs at much lower Zn2+ concentrations in NR1a NR2A receptors ( IC 50 in the nanomolar range ) than in NR1a NR2B receptors ( IC 50 in the micromolar range ) . ^^^ By analyzing the effects of Zn2+ on varied combinations of NR 1 ( NR1a or NR1b ) and NR 2 ( NR2A , NR2B , NR2C ) , we show that both the NR 1 and the NR 2 subunits contribute to the voltage independent Zn2+ inhibition . ^^^ We have observed further that under control conditions , i . e . , in zero nominal Zn2+ solutions , the addition of low concentrations of heavy metal chelators markedly potentiates the responses of NR1a NR2A receptors , but not of NR1a NR2B receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The N methyl D aspartate receptor subunits NR2A and NR2B bind to the SH 2 domains of phospholipase C gamma . ^^^ Immunoblot analysis with antibodies specific for individual NMDA receptor subunits showed that both NR2A and NR2B subunits bound to the SH 2 agarose . ^^^ Immunoprecipitation experiments confirmed that NR2A and NR2B were phosphorylated on tyrosine and further that tyrosine phosphorylation of each of the subunits was increased after incubation with ATP . ^^^ The results demonstrate that NMDA receptor subunits NR2A and NR2B will bind to the SH 2 domains of PLC gamma and that isolated synaptic junctions contain endogenous protein tyrosine kinase ( s ) that can phosphorylate both NR2A and NR2B receptor subunits , and suggest that interaction of the tyrosine phosphorylated NMDA receptor with proteins that contain SH 2 domains may serve to link it to signaling pathways in the postsynaptic cell . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
These experiments document an abrupt decrease in the NMDA receptor contribution to synaptic currents that occurs before eye opening and is closely associated with changes in NR 1 protein , rapidly rising levels of the NMDA receptor subunit NR2A , and decreasing levels of NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have therefore examined the effects of transient cerebral ischemia on the tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B in different regions of the rat brain . ^^^ The tyrosine phosphorylation of NR2A and NR2B subunits was examined by immunoprecipitation with anti tyrosine phosphate antibodies followed by immunoblotting with antibodies specific for NR2A or NR2B , and by immunoprecipitation with subunit specific antibodies followed by immunoblotting with anti phosphotyrosine antibodies . ^^^ Transient ischemia followed by reperfusion induced large ( 23 29 fold relative to sham operated controls ) , rapid ( within 15 min of reperfusion ) , and sustained ( for at least 24 h ) increases in the tyrosine phosphorylation of NR2A and smaller increases in that of NR2B in the hippocampus . ^^^ The enhanced tyrosine phosphorylation of NR2A or NR2B may contribute to alterations in NMDA receptor function or in signaling pathways in the postischemic brain and may be related to pathogenic events leading to neuronal death . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( RT PCR ) amplification was used to quantify the altered expression of the N methyl D aspartate ( NMDA ) NR2A , NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen glucose deprivation . ^^^ Ninety minutes after re oxygenation and return to 10 mM glucose , there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole , as well as in the selectively vulnerable CA 1 region and the resistant CA 3 and dentate gyrus regions . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using chimeric NR2A / NR2B subunits , we have located a region of NR2B ( amino acids 138 238 ) which regulated glycine independent polyamine stimulation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The mRNA expression levels for the NR2A and NR2B subunits of NMDA receptors changed to different degrees within different subregions of the hippocampus after reperfusion with respect to sham operated control . ^^^ At the protein level , a significant decrease in the amount of NR2A / NR2B immunoreactivity in the total hippocampus was observed at both 6 and 24 h after reperfusion compared with sham control . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Molecular dissection of native mammalian forebrain NMDA receptors containing the NR 1 C2 exon : direct demonstration of NMDA receptors comprising NR 1 , NR2A , and NR2B subunits within the same complex . ^^^ The purified receptor subpopulation showed immunoreactivity with anti NR 1 C2 , anti NR 1 N1 , anti NR 1 C2 ' , anti NR2A , and anti NR2B NMDA receptor antibodies . ^^^ The NR 1 C2 receptor subpopulation was subjected to immunoprecipitation using anti NR2B antibodies and the resultant immune pellets analysed by immunoblotting where anti NR 1 C2 , anti NR 1 C2 ' , anti NR2A , and anti NR2B immunoreactivities were all found . ^^^ Of this , 87 % ( i . e . , 40 % of total ) were NR 1 C2 / NR2B receptors and 13 % ( 6 % of total ) were NR 1 C2 / NR2A / NR2B , thus identifying the triple combination as a minor receptor subset . ^^^ These results demonstrate directly , for the first time , the coexistence of the NR2A and NR2B subunits in native NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Brief application of an NMDA / glycine solution to cells markedly increased intracellular calcium in cells transfected with NR1 / NR2A , NR1 / NR2B , or NR1 / NR2A / NR2B as measured by fura 2 calcium imaging . ^^^ The NR2B selective antagonist ifenprodil inhibited responses in cells transfected with NR1 / NR2B or NR1 / NR2A / NR2B , but not NR1 / NR2A subunits . ^^^ Increasing the transfection ratio of NR2B versus NR2A subunit in NR1 / NR2A / NR2B transfected cells greatly increased their ifenprodil sensitivity . ^^^ HEK 293 cells transfected with NR1 / NR2A / NR2B subunits showed an enhanced sensitivity to ifenprodil following a 24 h exposure to concentrations of ethanol of 50 mM and greater . ^^^ The enhanced ifenprodil sensitivity following ethanol exposure was not associated with changes in NR 1 , NR2A , or NR2B immunoreactivity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Single channel recordings were obtained from Chinese hamster ovary cells transfected with the N methyl D aspartate ( NMDA ) receptor subunit NR 1 in combination with NR2A , NR2B , NR2C , or NR2A / NR2B . ^^^ CP dramatically reduced both the open dwell time and frequency of channel opening of NR1 / NR2B containing receptors , but only modestly inhibited NR1 / NR2A and NR1 / NR2C channel activity . ^^^ A small number of patches obtained from cells transfected with NR1 / NR2A / NR2B had channels with properties intermediate to NR1 / NR2A and NR1 / NR2B receptors , including insensitivity to CP block but redox properties similar to NR1 / NR2B , consistent with the coassembly of NR2A with NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptor subtypes expressed in Xenopus oocytes were blocked with IC 50 values of 0 . 009 microM and 52 microM for the subunit combinations NR1C & NR2B and NR1C & NR2A , respectively , which indicated a > 5000 fold selectivity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The NR2A and NR2C subunit mRNAs were less widely distributed , whereas little NR2D mRNA was detected and only rare cells contained NR2B mRNA . ^^^ No significant changes were apparent in NR2A , NR2B and NR2D mRNA levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Identification of amino acid residues of the NR2A subunit that control glutamate potency in recombinant NR1 / NR2A NMDA receptors . ^^^ By analysis of 10 mutant NMDA receptors , we now show that residues on the NR2A subunit control glutamate potency in recombinant NR1 / NR2A receptors , without affecting glycine potency . ^^^ One NR2A mutant , NR2A ( T671A ) , had an EC 50 for glutamate 1000 fold greater than wild type and a 255 fold reduced affinity for APV , yet it had single channel openings very similar to those of wild type . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using reverse transcription ( RT ) competitive PCR we studied the expression of NR2A and NR2B mRNA in neocortical neurones differentiating in culture . ^^^ We found a developmental increase in NR2A mRNA expression in relation to NR2B mRNA expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Mouse monoclonal antibodies were raised against bacterially expressed protein sequences of the NR2A , NR2B , NR2C and NR2D subunits of the rat NMDA receptor . ^^^ In distribution studies , NR2A immunoreactive protein ( IRP ) was located throughout the adult rat brain , NR2B IRP was primarily in the forebrain , NR2C IRP was predominantly in the cerebellum and NR2D IRP was mainly found in the thalamus , midbrain and brainstem . ^^^ Whereas NR2A and NR2C IRPs increased during rat brain post natal development , NR2B and NR2D IRPs were abundant at birth and declined with age , especially in cerebellum . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition , the NR2A subunit , but not the NR2B subunit , was selectively detected on pyramidal cell dendrites in the stratum lucidum of CA 3 , suggesting a selective targeting to sites of mossy fiber input . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Film autoradiograms demonstrated a distinct pattern of hybridization signal in the hippocampal complex and the neocortex with probes for NR 1 , NR2A , and NR2B mRNA . ^^^ NR2A and NR2B mRNAs were expressed in cortical areas and layers . ^^^ Pyramidal cells in both hippocampus and neocortex express NR 1 , NR2A , NR2B , and , to a lesser extent , NR2D mRNA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Deactivation time course recorded in response to fast glutamate pulses were studied in isolated and lifted cells , as well as in outside out membrane patches excised from cells expressing recombinant NR 1 subunits in combination with the NR2A , NR2B , NR2C , or NR2D NMDA receptor subunits . ^^^ Currents generated by NR1 / NR2A channels displayed double exponential deactivation time course being faster than that in NR1 / NR2B or NR1 / NR2C channels . ^^^ Additionally , recovery from desensitization was slower for NR1 / NR2B than for NR1 / NR2A channels . ^^^ The average deactivation time course of responses to brief L glutamate applications in cells where NR1 / NR2A / NR2B cDNAs were cotransfected at variable ratio was intermediate between those of the NR1 / NR2A and NR1 / NR2B channels . ^^^ This produced the result that many cells were characterized by deactivation time course and haloperidol sensitivities of separate NR1 / NR2A and NR1 / NR2B subunit heteromers . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Although a few studies have examined the composition of NMDA receptors containing NR 1 , NR2A , and NR2B , the composition of native NMDA receptors that incorporate the NR2D subunit is not known . ^^^ The goal of the current study was to examine the subunit composition of native NMDA receptors that contain the NR2D subunit in the rat central nervous system by immunoprecipitation of assembled NMDA receptors from rat brain tissues using specific antibodies against NR 1 , NR2A , NR2B , and NR2D subunits . ^^^ In addition , the NR2D subunit forms a heteromeric assembly with NR 1 , as well as with NR2A and / or NR2B subunits , reflecting ternary complex formation . ^^^ Finally , a binary complex composed of only NR1 / NR2D subunits was found in the thalamus but not in the midbrain , where the complexes always contained either NR2A or NR2B , demonstrating that in the central nervous system , different subtypes of NR2D containing NMDA receptors are present that vary in spatial expression , perhaps indicating distinct physiological and behavioral roles . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we describe a novel NR2B selective NMDA antagonist , 4 3 [ 4 ( 4 fluoro phenyl ) 3 , 6 dihydro 2H pyridin 1 yl ] 2 hydroxy propoxy  benzamide ( Ro 8 4304 ) , which exhibits > 100 fold higher affinity for recombinant NR 1 ( 001 ) / NR2B than NR 1 ( 001 ) / NR2A receptors . 2 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analyses on the differential distribution of NR2A and NR2B subunits in the adult rat brain . ^^^ Quantitative immunoblot analyses were carried out to study the distribution of N methyl D aspartate ( NMDA ) receptor subunit 2A and 2B ( NR2A and NR2B , respectively ) at the protein level in the adult rat brain . ^^^ The NR2B was more dramatic in differential distribution than the NR2A . ^^^ Our data shows that NR2A and NR2B subunits are differentially distributed in the brain in an overlapping manner , and that the major phosphotyrosine containing protein of 180 kDa in forebrain is the NR2B . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Examination of the GluR 2 flip and flop , NR 1 , NR2A and NR2B subunit gene transcripts showed a qualitatively similar distribution in both the neocortex and hippocampus of MAM and control rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The absence of NMDA and AMPA receptors in rat and human CEC was further confirmed by the lack of immunocytochemical staining of cells by antibodies specific for the AMPA receptor subunits GluR 1 , GluR2 / 3 , and GluR 4 and the NMDA receptor subunits NR 1 , NR2A , and NR2B . ^^^ Primary rat cortical neurons were found to express GluR 1 to GluR 3 and NR 1 , NR2A , and NR2B by both immunocytochemistry and RT PCR and KA 1 , KA 2 , GluR 5 , GluR 6 , and GluR 7 by RT PCR . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The acute effects of subcutaneously injected morphine on transcripts of the NMDA receptor subunits NR 1 , NR2A and NR2B in certain areas of the central nervous system of male rats were examined by Northern blot analysis . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The protein levels of the NR 1 and NR2B , but not NR2A , subunits were significantly increased in diazepam withdrawn rats compared to those in control rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The yeast Saccharomyces cerevisiae was used for expressing the genes encoding the ionotropic N methyl D aspartate ( NMDA ) receptor subunits from rats ( NR1a , NR2A , NR2C ) and mice ( NR2B ) . ^^^ Pharmacological characterization of crude membrane preparations of the recombinant yeast cells showed saturable binding of the glycine antagonist [ 3H ] MDL 105 , 519 with Kd values of 56 . 9 + / 6 . 19 nM ( NR1a / NR2A ) , 26 . 72 + / 2 . 13 nM ( NR1a / NR2B ) , and 21 . 22 + / 1 . 64 nM ( NR1a / NR2C ) , and bound capacities of 17 . 94 + / 1 . 24 pmol / mg membrane protein ( NR1a / NR2A ) , 11 . 45 + / 0 . 67 pmol / mg ( NR1a / NR2B ) , and 16 . 15 + / 0 . 86 ( NR1a / NR2C ) pmol / mg . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The pattern of NR 1 , NR2A , NR2B , and NR2C subunit mRNA in situ hybridization was consistent with previous studies . ^^^ Lead exposure resulted in significant and opposite effects in NR 1 and NR2A subunit mRNA expression with no changes in NR2B or NR2C subunit expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The authors investigated the gene expression of the NR2A and NR2B subunits of N methyl D aspartate ( NMDA ) receptor and the functional electrophysiologic activity of NMDA receptor complexes in the vulnerable CA 1 and less vulnerable dentate gyrus subfields of the rat hippocampus at different times after transient cerebral ischemia . ^^^ Decreased expression for both subtypes was observed in both the CA 1 subfield and dentate granule cell layer at early times after challenge ; however , the decreased expression in the dentate granule cell layer was reversible because mRNA levels for both the NR2A and NR2B subtypes recovered to , or surpassed , sham operated mRNA levels by 3 days postchallenge . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Distribution and expression of the subunits of N methyl D aspartate ( NMDA ) receptors ; NR 1 , NR2A and NR2B in hypoxic newborn piglet brains . ^^^ The purpose of this study was to identify the distribution and the expression of the NR 1 , NR2A and NR2B subunits of the NMDA receptor after cerebral hypoxia . ^^^ Tissue samples from the nine different regions of newborn piglet brain were obtained , and the protein amount of the NR 1 , NR2A , and NR2B subunits measured by immunoblot using the antibody to the NR 1 , NR2A , and NR2B subunits . ^^^ But the expression of the NR 1 , NR2A and NR2B subunits were not significantly different between the control and the hypoxic group , 1 h after hypoxic exposure , indicating no changes in the protein amount of NMDA receptor subunits . ^^^ These results show a significantly higher amount of the NR 1 , NR2A and NR2B subunits in the hippocampus and the cerebral cortex of newborn brains , indicating that these structures could be highly vulnerable to excitotoxicity in the newborn brain . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Spectrin is reported here to interact selectively in vitro with the C terminal cytoplasmic domains of the NR1a , NR2A and NR2B subunits of the NMDA R but not with that of the AMPA receptor GluR 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Human embryonic kidney ( HEK ) 293 cells containing NR 1 1a or NR 1 1b with either the NR2A or NR2B subunits did not differ in their EtOH sensitivity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We investigated the effect of chronic ethanol administration and its withdrawal on the polypeptide levels of NMDA receptor subunits such as NR 1 , NR2A , and NR2B in the rat cerebral cortex and hippocampus using Western blot analysis technique . ^^^ Our results indicate that chronic ethanol treatment upregulates NMDA receptor subunits NR 1 , NR2A , and NR2B ( approximately 35 % ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we show that NR2B , but not NR2A or NR 1 , subunits of NMDA receptors are responsible for autophosphorylation dependent targeting of CaMKII . ^^^ Autophosphorylation induces direct high affinity binding of CaMKII to a 50 amino acid domain in the NR2B cytoplasmic tail ; little or no binding is observed to NR2A and NR 1 cytoplasmic tails . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Our data show that inclusion of exon 5 into the NR 1 subunit increases the IC 50 for voltage independent Zn2+ inhibition from 3 fold to 10 fold when full length exon 22 is also spliced into the mature NR 1 transcript and the NMDA receptor complex contains the NR2A or NR2B subunits ; exon 5 has little effect on Zn2+ inhibition of receptors that contain NR2C and NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Potency and subunit selectivity were assayed by electrical recordings in Xenopus oocytes expressing three binary combinations of cloned rat NMDA receptor subunits : NR1A expressed in combination with either NR2A , NR2B , or NR2C . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The percent increase in NR2A mRNA was larger than the percent change in NR 1 , NR2B or NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Consistent with the electrophysiology and Western blot analysis , reverse transcriptase polymerase chain reaction ( RT PCR ) amplification revealed that BDNF caused a significant increase in relative NR2A subunit expression , a significant decrease in relative NR2B subunit expression and no change in relative NR2C subunit expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blot and RNase protection analyses were used to determine the expression of the various NMDA receptor subunits ( NR 1 , NR2A , NR2B , and NR2C ) after BDNF or FGF 2 treatment . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We also observed a transient increase in both NR2B and NR2A protein levels 20 min post tetanus that returned to control levels by 4 h . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To understand its molecular anatomical basis , we produced affinity purified antibodies against the GluRepsilon 1 ( NR2A ) , GluRepsilon 2 ( NR2B ) , and GluRzeta 1 ( NR 1 ) subunits of the N methyl D aspartate ( NMDA ) receptor channel , and determined their distributions in the mouse hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analysis , using antibodies against distinct N methyl D aspartic acid ( NMDA ) receptor subunits , illustrated that the NR2A and NR2B subunit proteins have developmental profiles in cultured cortical neurons similar to those seen in vivo . ^^^ Clusters of NR 1 and NR2B subunits were observed as early as DIV 3 , while NR2A clusters were rarely observed before DIV 10 . ^^^ At DIV 18 , NR2B clusters partially co localize with those of NR2A subunits , but NR2B clusters always co localize with those of NR 1 subunits . ^^^ Furthermore , while NR2B subunit clusters were seen both at synaptic and extrasynaptic sites , NR2A clusters occurred almost exclusively in front of synaptophysin labelled boutons . ^^^ Our data indicate that excitatory synapse formation occurs when NMDA receptors comprise NR 1 and NR2B subunits , and that NR2A subunits cluster preferentially at synaptic sites . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In forebrain , the pattern of NMDA specific [ 3H ] homoquinolinate binding paralleled NR2B and not NR2A distribution . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A or NR2B , in combination with NR 1 ( 011 ) , conveyed enhancement whereas NR2C and NR2D conveyed suppression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The association of NMDA receptor subunits and CaMKII was assessed by immunoprecipitating PSD proteins with antibodies specific for NR2A / B and CaMKII : CaMKII coprecipitated with NR2A / B and NR 1 but not with other glutamate ionotropic receptor subunits , such as GluR 1 and GluR 2 3 . ^^^ Here we report that CaMKII binds to NR2A and NR2B subunits of NMDA receptors in PSD isolated from cortex and hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The neuropeptide release elicited by D serine was strongly inhibited by ifenprodil ( 0 . 3 microM ) and by Zn2+ ions ( 50 nM ) , selective ligands at the NR2B and NR2A subunits of NMDA receptors , respectively . ^^^ These receptors may display the triple subunit combination NR1 / NR2A / NR2B . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we extend those earlier studies by measuring levels of subunit protein for NMDAR 1 , NR2A , and NR2B in the hippocampus of control and kindled animals , 28 days after the last kindled seizure . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We investigated the influence of centrally administered pentobarbital on the regulation of mRNA levels of the family of NMDA receptor 2 ( NR 2 ) subtypes ( NR2A , NR2B , and NR2C ) by in situ hybridization histochemistry in rat brain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To address this central issue of ischemic brain damage more directly , we used mutant mice deficient in the GluRepsilon 1 ( NR2A ) subunit of NMDA receptor with or without additional heterozygous mutation in the GluRepsilon 2 ( NR2B ) subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To determine whether tyrosine phosphorylation of NMDAR subunits contributes to the apparent long term enhancement in synaptic efficacy of these receptors , we examined the effect of unilateral nigrostriatal dopamine system ablation with 6 hydroxydopamine followed by twice daily treatment with l DOPA on the phosphorylation state of rat striatal NR2A and NR2B subunits . ^^^ Concurrently , tyrosine phosphorylation of NR2A and especially of NR2B subunits increased ipsilateral to the lesion ( 20+ / 5 % and 46+ / 7 % of intact striatum , respectively ; p < 0 . 01 ) without attendant changes in subunit protein levels . ^^^ Selective blockade of NR2B subunits with ACEA 10 1244 , but not of NR2A subunits with MDL 100 , 453 , reversed the l DOPA induced response alterations . ^^^ The intrastriatal injection of a tyrosine kinase inhibitor , genistein , at a dose ( 2 . 0 microg ) that normalized the response shortening , attenuated the NR2A and NR2B phosphorylation increase by about 12 % and 24 % , respectively ( p < 0 . 01 ) . ^^^ Taken together , these results suggest that augmented tyrosine phosphorylation of NR2B subunits , alone or in combination with the smaller rise in NR2A subunit phosphorylation , contributes to the apparent enhancement in striatal NMDAR sensitivity and thus to the plastic alterations in dopaminergic responses in l DOPA treated parkinsonian rats . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The presence of NMDA receptor protein was determined by Western blot analysis for the NR 1 , NR2A , and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast to previous reports , this study determines the importance of individual exons in the mechanism underlying the potentiation process by examining the complete set of eight naturally occurring splice variants expressed in Xenopus oocytes both as homomers and as heteromeric NR1 / NR2A or NR1 / NR2B complexes . ^^^ The effectiveness of the C 1 exon to reduce the higher form of potentiation is modulated by heteromeric assemblies with NR2A heteromers yielding smaller levels of potentiation and a larger C 1 exon effect compared with NR2B heteromers . ^^^ This study refines the region of the NR 1 subunit involved in a modulation crucial to the function of NMDA receptors and provides evidence that the NR2A and NR2B subunits realize this modulation differentially . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition to high levels of NR 1 subunit mRNA , dorsolateral nucleus neurons moderately expressed NR2A and NR2B subunit mRNAs . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
CaMKII dependent phosphorylation of NR2A and NR2B is decreased in animals characterized by hippocampal damage and impaired LTP . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using in situ hybridization techniques , the effects of chronic morphine treatment on the expression of mRNAs encoding the NMDA receptor subunits NRI , NR2A , and NR2B were investigated . ^^^ The expression of NR2A and NR2B subunit mRNAs did not change after morphine treatment in any brain region . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ischemic preconditioning selectively induces a decrease in the levels of the NR2A and NR2B subunits and a modest decrease in the levels of NR 1 subunit proteins in the synaptosomal fraction of the neocortex but not hippocampus after the second lethal ischemia . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The relative amounts of NR2A , NR2B , NR2C corresponded to approximately 1 : 2 : 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At the same time , levodopa treatment elevated serine phosphorylation of striatal NR2A ( p < 0 . 02 ) , but not that of NR2B subunits , without associated changes in subunit protein levels . ^^^ Chronic treatment with SKF 38393 increased NR2A ( p < 0 . 0001 ) but decreased NR2B ( p < 0 . 004 ) serine phosphorylation . ^^^ In contrast , chronic quinpirole treatment had no effect on NR2A but increased NR2B phosphorylation ( p < 0 . 0001 ) . ^^^ The acute intrastriatal injection of the CaMKII inhibitor KN 93 ( 1 . 0 micrograms ) not only normalized the levodopa induced motor response alterations but also attenuated the D 1 and D 2 receptor mediated serine phosphorylation of NR2A and NR2B subunits , respectively ( p < 0 . 02 ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analysis of NMDA ( NR 1 , NR2A , NR2B , and NR2D ) , AMPA ( GluR 1 and GluR2 / 3 ) , and kainate ( GluR6 / 7 ) subunit polypeptides in 3 , 5 , 8 , 10 , and 12 day old cultures showed that NMDA receptor subunits NR 1 , NR2A , and NR2B and AMPA receptor subunits GluR2 / 3 progressively increased as a function of time , whereas levels of NMDA subunit NR2D were high at day 3 and progressively declined to barely detectable levels by day 12 . ^^^ However , exposure of low glutamine cultures to 100 mM ethanol for 4 days ( starting at culture day 9 ) significantly increased the levels of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) and AMPA receptor subunits ( GluR 1 and GluR2 / 3 ) , but had no effect upon kainate receptor subunits ( GluR6 / 7 ) or the synapse associated proteins synapsin 1 and PSD 95 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Lateral hypothalamic NMDA receptor subunits NR2A and / or NR2B mediate eating : immunochemical / behavioral evidence . ^^^ To begin to address this , we detected LHA NR2A and NR2B subunits by immunoblotting and NR2B subunits by immunohistochemistry using subunit specific antibodies . ^^^ To help determine whether NMDA Rs mediating feeding might contain these subunits , we conducted behavioral studies using LHA administered ifenprodil , an antagonist selective for NR2A and / or NR2B containing NMDA Rs at the doses we used ( 0 . 001 100 nmol ) . ^^^ Collectively , these results suggest that LHA NMDA Rs , some of which contribute to feeding control , are composed of NR2A and / or NR2B subunits , and implicate NR2A and / or NR2B linked signal transduction in feeding behavior . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 , NR2A , and NR2B subunit protein levels were measured in rat hippocampus and cortex at post natal days ( PND ) 7 , 14 , 21 , and 28 by Western blot and densitometric analysis . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Insulin causes a transient tyrosine phosphorylation of NR2A and NR2B NMDA receptor subunits in rat hippocampus . ^^^ Here we report that insulin causes a transient phosphorylation of NR2A and NR2B NMDA receptor subunits on tyrosine residues . ^^^ NR2A and NR2B subunits were immunoprecipitated and probed for tyrosine phosphorylation . ^^^ Insulin incubation of hippocampal slices for 20 min elicited an increase in tyrosine phosphorylation to 176 + / 16 % ( NR2A ) and 203 + / 15 % ( NR2B ) of control levels . ^^^ In contrast , 60 min of insulin incubation did not alter NR 2 tyrosine phosphorylation levels ( NR2A : 85 + / 13 % of control ; NR2B : 93 + / 10 % of control ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We found an increase in expression of the NR2A subunit , and a decrease in expression of the NR2B subunit of the NMDA receptor in primary cultures of cerebellar granule neurons over time in culture , with no significant change in NR 1 expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
PSD 95 ( SAP 90 ) , SAP 102 and Chapsyn 110 ( PSD 93 ) are members of the membrane associated guanylate kinase family , and interact with N methyl D aspartate ( NMDA ) receptor NR2A ( GluRepsilon 1 ) and NR2B ( GluRepsilon 2 ) subunits and with Shaker type K+ channel subunits to cluster into a channel complex . ^^^ In the early postnatal period when active synaptogenesis takes place , both mRNAs became elevated and concentrated in the telencephalon and cerebellar granular layer , where NR2A and / or NR2B subunit mRNAs are abundantly expressed . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Polyamine agonists did not affect [ 125I ] MK 801 binding in NR1a / NR2A , whereas spermine and spermidine did produce enhancement , and , at higher concentrations , inhibition of binding in NR1a / NR2B . ^^^ However , 1 , 5 ( diethylamino ) piperidine inhibited [ 125I ] MK 801 binding ( IC 50 = 8 . 0 microM ) in NR 1 : NR2A receptors and produced a small increase followed by a modest decrease in binding to NR1a / NR2B receptors . ^^^ Similar effects were demonstrated in both NR 1 : NR2A and NR 1 : NR2B receptors ( IC 50 = 8 . 4 and 14 . 1 microM , respectively ) and agonists decreased the affinity of arcaine in both receptor preparations . ^^^ Furthermore , native NMDA receptors pharmacology can not be modeled by simple NR 1 : NR2A or NR 1 : NR2B combinations . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A subunit levels were significantly increased only in hippocampus from ethanol dependent male rats , whereas NR2B subunit levels significantly increased in cerebral cortex of both female and male rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
AMPA / kainate and NR 1 , NR2A , and NR2B receptor transcripts show higher expression than other transcripts . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The expression of NMDA receptor subunits NR 1 , NR2A , and NR2B was examined in animals ranging in age from postnatal day 16 to adult using Western blotting . ^^^ We observed a remarkable increase in NR 1 and NR2A , but not NR2B , expression after eye opening . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blot analysis indicated that NMDAR subunit composition changed from a combination of NR2A and NR2B containing NMDARs to primarily NR2A with increasing days in vitro ( DIV ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptor subunit gene expression in the rat brain : a quantitative analysis of endogenous mRNA levels of NR1Com , NR2A , NR2B , NR2C , NR2D and NR3A . ^^^ Whiting , Pharmacological properties of recombinant human N methyl d aspartate receptors comprising NR1a / NR2A and NR1a / NR2B subunit assemblies expressed in permanently transfected mouse fiberblast cells , Mol . ^^^ The present study was undertaken to examine the level of gene expression for the NR 1 , NR2A , NR2B , NR2C , NR2D and NR3A receptor subunits in isolated regions of rat brain using the nuclease protection assay . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Accordingly , NR 1 and NR2A as well as GluR 1 , GluR2 / 3 , PSD 95 and alphaCaMKII protein concentrations in post synaptic densities were the same in both control and diabetic rats , whereas the immunoreactivity for NR2B was reduced by about 40 % . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the present study , we employed light and electron microscopic immunocytochemistry to study the expression of NR2A and NR2B ( NR2A / B ) protein in somatic sensory cortex of adult rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blot analyses using antibodies against different subunits of 2 amino 3 hydroxy 5 methylisoxazole 4 propionic acid ( AMPA ) and N methyl D aspartate ( NMDA ) types of ionotropic glutamate receptors indicate that AMPA receptor subunits ( GluR 1 , GluR2 / 3 , and GluR 4 ) , but not NMDA receptor subunits ( NR 1 and NR2A ) , are proteolytically cleaved after exposure of hippocampal neurons to apoptotic insults , including Abeta , and that the caspase inhibitor zVAD fmk suppresses such cleavage . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptors are heteromeric complexes of NR1A combined with NR2A , NR2B , NR2C , and / or NR2D subunits . ^^^ In this report , we provide evidence for the first time that there is also a significant difference in peak channel open probability ( P ( o ) ) between NMDA receptors composed of NR1A / NR2A and those of NR1A / NR2B subunits . ^^^ First , whole cell patch clamp recordings from human embryonic kidney ( HEK ) 293 cells expressing NMDA receptors revealed that NR1A / NR2A mediated peak current densities are approximately four times larger than those of NR1A / NR2B . ^^^ Our results indicate that peak P ( o ) is significantly higher ( twofold to fivefold ) for NR1A / NR2A than NR1A / NR2B , with estimated values of approximately 0 . 35 and 0 . 07 , respectively . ^^^ These results suggest that a change in the relative expression levels of NR2A and NR2B can regulate peak amplitude of NMDA receptor mediated excitatory postsynaptic potentials and therefore may play a role in mechanisms underlying synaptic plasticity . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N methyl D aspartate ( NMDA ) subunits NR2A and NR2B , bind to the PSD protein called PSD 95 , which in turn binds neuroligins , providing a handle for interacting with neurexin , located in the plasma membrane at the presynaptic active zone . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Transient ischemia increases tyrosine phosphorylation of N methyl D aspartate ( NMDA ) receptor subunits NR2A and NR2B in the rat hippocampus . ^^^ The NR2A and NR2B bound to each of the SH 2 domains and binding was increased approximately twofold after ischemia and reperfusion . ^^^ Ischemia induced a marked increase in the tyrosine phosphorylation of several proteins in the postsynaptic density ( PSD ) , including NR2A and NR2B , but had no effect on the amounts of individual NMDA receptor subunits in the PSD . ^^^ The ischemia induced increase in the interaction of NR2A and NR2B with the SH 2 domains of Src and Fyn suggests a possible mechanism for the recruitment of signaling proteins to the PSD and may contribute to altered signal transduction in the postischemic hippocampus . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Potency and subunit selectivity were assayed by electrical recordings in Xenopus oocytes expressing three binary combinations of cloned rat NMDA receptor subunits : NR1A expressed in combination with either NR2A , NR2B , or NR2C . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Thus , human embryonic kidney 293 cells were transfected with the NR 1 1a and NR2A NMDA receptor subunits in combination with both FLAG and c Myc epitope tagged NR2B subunits . ^^^ Immunoblotting of the double immunopurified NR2A / NR2B ( FLAG ) containing material demonstrated the presence of anti NR 1 , anti NR2A , anti FLAG , and , more important , anti c Myc antibody immunoreactivities . ^^^ NR2A / NR2B ( FLAG ) / NR2B ( c Myc ) , within the same NMDA receptor complex . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Compared with autopsy hippocampi , non HS and HS patients showed increased NR2A and NR2B hybridization densities per dentate granule cell . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDARs at birth are comprised of NR2B and NR 1 subunits , and , over the first 5 postnatal weeks , there is a progressive inclusion of the NR2A subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The proportion of cells expressing the NR2A and NR2B subunits remained relatively constant throughout the first five postnatal weeks . 7 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
For the PB / KF , we found in addition a moderate expression of NR2A mRNA in the internal lateral PB and of NR2B mRNA in the external lateral PB . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Analysis of NMDA receptor subunits shows a persistent , approximately 30 50 % reduction of NR 1 , NR2A , and NR2B subunits at postnatal day 90 in the barrel field cortex . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
With voltage clamp recording of Xenopus oocytes expressing heteromeric NMDA receptors from cloned NR 1 and NR 2 subunit RNAs , Con R exhibited the following order of preference for NR 2 subunits : NR2B approximately NR2A > NR2C > > NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The postsynaptic protein PSD 95 binds to NMDA receptor subunits NR2A and NR2B and to signaling molecules such as neuronal nitric oxide synthase and p135synGAP . ^^^ The association between PSD 95 and NR2A and NR2B , as indicated by coimmunoprecipitation , was less in postischemic samples than in sham operated controls . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Similarly , mRNAs encoding all four alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ( AMPA ) type glutamate receptor subunits and the NR 1 and NR2A , 2B , and 2D subunits of the NMDA type glutamate receptors were detected , suggesting that various combinations of these subunits mediate the cellular response to synaptically released glutamate . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In lesioned striatum , the abundance of NR 1 and NR2B in striatal membranes was decreased to 68 + / 3 . 2 and 62 + / 4 . 4 % , respectively , relative to the unlesioned striata , whereas the abundance of NR2A was unchanged . ^^^ Coimmunoprecipitation of NMDA receptors under nondenaturing conditions revealed that these changes reflected a selective depletion of receptors composed of NR1 / NR2B , without alteration in receptors composed of NR1 / NR2A . ^^^ The phosphorylation of NR 1 protein at serine 890 and serine 896 , but not at serine 897 , and the tyrosine phosphorylation of NR2B but not NR2A were decreased in the membrane fraction of the lesioned striatum . ^^^ Chronic treatment of lesioned rats with L dopa normalized the alterations in the abundance and subunit composition of the NMDA receptors in striatal membranes , and produced striking hyperphosphorylation , both of NR 1 at serine residues , and NR2A and NR2B at tyrosine residues . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
From Western blots of hippocampus and immunogold analysis of CA 1 synapses , the high expression of NR2B at P 2 coincides with the high level of SAP 102 at synapses , whereas the later expression of NR2A coincides with that of PSD 93 and PSD 95 . ^^^ To determine whether the changes in PSD 93 / 95 and SAP 102 reflect preferred associations with NR2A and NR2B , respectively , we measured co immunoprecipitation in the adult hippocampus . ^^^ These studies suggest that there is a preference for complexes of NR2A / PSD 93 / 95 and NR2B / SAP 102 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoreactive bands corresponding to the NMDA receptor subunits NR 1 , NR2A , NR2B , NR2C and NR2D were demonstrated by immunoblotting at the earliest between 4 . 5 and 7 weeks and increasing concentrations were seen up to 11 weeks of gestation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Quantitative reverse transcriptase polymerase chain reaction was used to analyze the relative expressions of NR 1 , NR2A , NR2B , NR2C , NR2D , and NR 3 subunits of the NMDA receptor in the piriform , entorhinal , visual , and motor cortices as well as in the olfactory bulb of adult rat . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Consistently , exposure to insulin increased the level of the NR2A subunit of the NMDA receptor without much altering NR 1 or NR2B levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Felbamate produced a rapid , concentration dependent block of currents evoked by 50 microM NMDA and 10 microM glycine in human embryonic kidney 293 cells expressing the rat NR1a subunit , and either the NR2A , NR2B or NR2C subunits ; the IC 50 values for block were 2 . 6 , 0 . 52 and 2 . 4 mM , respectively ( holding potential , 60 mV ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Double immunostaining revealed that many of the constitutively expressed Zif 268 positive neurons were GABAergic but very few were cholinergic or neuronal nitric oxide synthase ( nNOS ) positive , and some of the Zif 268 positive neurons were also immunopositive for a glutamate NMDA receptor subunit NR 1 or NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Postnatal development of NR 1 , NR2A and NR2B immunoreactivity in the visual cortex of the rat . ^^^ The NMDAR subunits NR 1 , NR2A and NR2B are developmentally regulated , and it has been proposed that developmental changes in their expression may underlie developmental changes in cortical plasticity . ^^^ We hypothesized that there will be significant age dependent change in expression of NR 1 , NR2A or NR2B between P 22 , when the cortex is plastic , and P 90 , when it is not . ^^^ We applied specific antibodies recognizing NR 1 , NR2A and NR2B to the primary visual cortex at P 14 , P 22 , P 30 , P 45 and P 90 . ^^^ In contrast , NR2A IR and NR2B IR were positively correlated . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of several PSD proteins , including the N methyl D aspartate ( NMDA ) receptor subunits NR2A and NR2B , was enhanced relative to shams after 20 minutes of reperfusion and underwent a further increase between 20 minutes and 6 hours . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the H ras null mutant hippocampus , the tyrosine phosphorylation of NR2A ( epsilon 1 ) and NR2B ( epsilon 2 ) subunits of NMDA receptors is increased , and , correspondingly , NMDA synaptic responses are selectively enhanced . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Finally , chronic MK 801 administration did not alter NMDA receptor NR 1 , NR2A , or NR2B subunit peptide expression in either the cerebral cortex or the hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunohistochemical localization of N methyl D aspartate receptor NR 1 , NR2A , NR2B and NR2C / D subunits in the adult mammalian cerebellum . ^^^ The distributions of the N methyl D aspartate ( NMDA ) receptor NR 1 , NR2A , NR2B and NR2C / D subunits were mapped in adult mouse cerebellum using subunit specific antibodies . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We now report that levels of AMPA receptor subunits GluR 1 and GluR 4 are rapidly decreased in cultured rat hippocampal neurons undergoing apoptosis in response to withdrawal of trophic support ( WTS ) , whereas levels of NMDA receptor subunits NR 1 , NR2A , and NR2B are unchanged . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Development of NR 1 , NR2A and NR2B mRNA in NR 1 immunoreactive cells of rat visual cortex . ^^^ In cells marked for N methyl D aspartate receptors ( NMDARs ) , we studied the relationship between the sensitive period for monocular deprivation and the expression of rat NMDAR subunits , NR2A and NR2B . ^^^ We then used in situ hybridization to label the same sections for NR2A or NR2B mRNA . ^^^ Staining for NR2B mRNA , but not for NR2A mRNA , decreased dramatically from P 22 to P 45 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In situ hybridization autoradiography was used to quantify mRNA for the NMDA receptor subunits NR 1 , NR2A , and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2B mRNA was expressed at low to moderate levels throughout the studied period whereas NR2A mRNA levels were low until P 21 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Developmental changes in the expression of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) in the cat visual cortex and the effects of dark rearing . ^^^ The present study used Western blots to determine changes in the level of expression of the three major NMDA receptor subunits , NR 1 , NR2A , and NR2B , in relation to the ' critical period ' in cat visual cortex . ^^^ NR2B showed a similar time course to NR2A , but the rise from 1 to 5 weeks was of lesser magnitude ( twofold ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA induced currents recorded from XENOPUS : oocytes injected with cRNA encoding the NMDA receptor subunits NR 1 , NR2A , and / or NR2B , mainly found in rat hypothalamus , were not affected by PKA activation but were increased by protein kinase C ( PKC ) stimulation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition , no training associated increase in the levels of NMDA NR2A and NR2B or AMPA GluR 2 / 3 subunits was observed at any timepoint tested . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 was the most abundant subunit mRNA ; NR2A , NR2B , and NR2D subunit mRNAs were also present , but were expressed in nucleus specific patterns . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
During cerebellar development , granule cells display well characterized changes in the expression of NMDA receptor ( NMDAR ) NR 2 subunits , switching from NR2B to NR2A and NR2C in mature cells . ^^^ Although various studies , including experiments on mutant mice with one or more NR 2 subunit types deleted , suggest that NR2A , NR2B , and NR2C subunits contribute to synaptic NMDARs , changes in the properties of the mossy fiber EPSC during development have not been fully evaluated . ^^^ Our data are consistent with the known switch from NR2B to NR2A subunits during the first two postnatal weeks , but suggest a gradual incorporation of the NR2C subunit that modifies Mg ( 2+ ) sensitivity and only later influences EPSC kinetics . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast , no changes in NR2A or NR2B subunit protein were observed in the same hippocampal homogenates . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 was found in two bands which can be related to the off and on signal pathways , whereas NR2A and NR2B were located in two bands within the off sublaminae of the IPL . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RESULTS : Ninety three percent of retinal ganglion cells expressed mRNA coding for NR 1 , 9 % for NR2A , 28 % for NR2B , 86 % for NR2C , and 84 % for NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Recombinant NR1a / NR2A ( T671A ) N methyl D aspartate ( NMDA ) receptor channels , which carry a point mutation in the putative glutamate binding site that reduces glutamate potency by around 1000 fold , have been expressed in Xenopus laevis oocytes and their single channel properties examined using patch clamp recording techniques . ^^^ The single channel data that we present in this paper support further the idea that the point mutation T671A in the NR2A NMDA receptor subunit affects mainly the ability of glutamate to remain bound to these channels . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Intracerebroventricular injection of the N terminal substance P fragment SP ( 1 7 ) regulates the expression of the N methyl D aspartate receptor NR 1 , NR2A and NR2B subunit mRNAs in the rat brain . ^^^ We have examined the effect of intracerebroventricular injections of SP ( 1 7 ) on the expression of the NMDA receptor subunits NR 1 , NR2A and NR2B mRNAs in the spinal cord and in discrete areas of the male rat brain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
As demonstrated by chronic blockade of postsynaptic glutamate receptors in vitro , the regulation of NR2A mRNA was strongly dependent on synaptic activity . ^^^ It is interesting that even relatively weak expression of NR2A mRNA was correlated with clearly reduced sensitivity of NMDA receptor mediated whole cell currents against the NR2B subunit specific antagonist ifenprodil . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We observed a gradual increase in GluR2 / 3 , NR2A , NR2B and NR2C levels of expression in the medial and lateral vestibular nuclei during the first 3 weeks of postnatal development . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
GluR 1 , GluR2 / 3 , GluR 4 , NR 1 ) appear to be stable for up to 18 h post mortem , the NR2A and NR2B subunits appear to be proteolyzed rapidly following death . ^^^ These results are consistent with the concept that the proteolytic products of NR2A and NR2B , although at smaller molecular sizes than the full length protein , are all identifiable on Western blots . ^^^ Modest decreases in NMDA receptor subunits NR 1 , NR2A , and NR2B were found in the hippocampus and in frontal cortex while little or no change in any of these subunits were documented in entorhinal cortex . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Message levels for the NMDA receptor subunits NR 1 , NR2A , and NR2B were not different between the chemically challenged neurons and control cells , whereas NR2C message was barely detectable in either group . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
ADNF causes secretion of neurotrophin 3 ( NT 3 ) , and both proteins regulate NMDA receptor subunit 2A ( NR2A ) and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We investigated the influence of centrally administered ginsenoside on the regulation of mRNA levels of the family of NMDA receptor subtypes ( NR 1 , NR2A , NR2B , NR2C ) by in situ hybridization histochemistry in the rat brain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Early post natal deafening decreased bilaterally the expression of mRNAs encoding NR 1 , NR2a , NR2b and flop isoforms of AMPA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Coexpression in human embryonic kidney ( HEK ) 293 cells of the postsynaptic density 95 protein ( PSD 95 ) with NMDA receptor NR2A or NR2B single subunits or NR 1 1a / NR2A and NR 1 1a / NR2B subunit combinations induced an approximately threefold increase in NR2A and NR2B subunit expression . ^^^ Deletion of the NR 2 C terminal ESDV motifs resulted in the loss of this increase following coexpression of NR 1 1a / NR2A ( Trunc ) and NR 1 1a / NR2B ( Trunc ) with PSD 95 . ^^^ Thus , coexpression of PSD 95 with NR 1 1a / NR2A results in a decreased sensitivity to L glutamate and an enhanced expression of NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2a and NR2b mRNA levels decreased significantly between cycling and acyclic rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The present study was designed to clarify the role of the NR 1 , NR2A and NR2B subunits of N methyl D aspartate receptors in the development of morphine induced place preference using specific antibodies to N methyl D aspartate receptor subunits in the mouse . ^^^ An i . c . v . injection with specific antibodies against the carboxyl terminal region of either NR 1 , NR2A or NR2B subunits caused the attenuation of seizures induced by i . v . infusion of N methyl D aspartate in the mouse . ^^^ Using western blotting , NR 1 , NR2A and NR2B subunits were found to be highly expressed in the cerebral cortex and hippocampus of the mouse brain , which are key areas in producing seizures regulated by N methyl D aspartate receptors . ^^^ Under these conditions , i . c . v . treatment with the antibody against NR2B subunits abolished the morphine induced place preference , whereas antibodies against NR 1 and NR2A subunits did not affect the rewarding effect of morphine . ^^^ Furthermore , the NR2B subunit protein was specifically up regulated in the limbic forebrain of morphine conditioned mice , whereas the protein levels of either NR 1 or NR2A subunits were not altered . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Protein levels and phosphorylation status of NMDA receptor subunits NR 1 , NR2A and NR2B were correlated with measurements of cognitive performance . ^^^ Reductions of NR2A ( 39 % , P < 0 . 05 ) and NR2B ( 31 % , P < 0 . 01 ) were found in entorhinal cortex . ^^^ Phosphorylated NR2A ( 30 % , P < 0 . 05 ) and NR2B ( 56 % , P < 0 . 01 ) were selectively reduced in entorhinal cortex in AD when compared to controls . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The effects of continuous infusion of butorphanol on the modulation of NMDA receptor subunit NR 1 , NR2A , NR2B , and NR2C gene expression were investigated by using in situ hybridization technique . ^^^ Continuous intracerebroventricular ( i . c . v . ) infusion with butorphanol ( 26 nmol / microl / h ) resulted in significant modulations in the NRI , NR2A , and NR2B mRNA levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Protein kinase C activation induces tyrosine phosphorylation of the NR2A and NR2B subunits of the NMDA receptor . ^^^ The aim of this study was to test whether this effect could be mediated by direct tyrosine phosphorylation of the NR2A or NR2B subunits of the receptor . ^^^ Following treatment of rat hippocampal CA 1 mini slices with 500 nM phorbol 12 myristate 13 acetate ( PMA ) for 15 min , samples were homogenized , immunoprecipitated with anti NR2A or NR2B antibodies and the resulting pellets subjected to Western blotting with antiphosphotyrosine antibody . ^^^ An increase in tyrosine phosphorylation of both NR2A ( 76 + / 11 % above control ) and NR2B ( 41 + / 11 % ) was observed . ^^^ PMA treatment also produced an increase in the phosphorylation of serine 890 on the NR 1 subunit , a known PKC site , at 5 min with phosphorylation returning to near basal levels by 10 min while tyrosine phosphorylation of NR2A and NR2B was sustained for up to 15 min . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In more severely injured rats , the chronic recovery of detrusor EUS coordination was very incomplete and correlated to decreased innervation of lower motoneurons by descending control pathways and their increased levels of mRNA for glutamate receptor subunits NR2A and GluR 2 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2C mRNA expression predominated over those of NR2A and NR2B mRNA in most neurones . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We found a significant decrease in the ratio of NR2A and NR2B subunit mRNA as compared to neurones from sham operated animals . ^^^ Thus , the observed perilesional changes in the NR2A / NR2B mRNA ratio appear to be subthreshold to result in significant changes in the functional properties of NMDA receptors . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
An intracerebroventricular ( i . c . v . ) treatment with a specific antibody against the carboxyl terminal region of the NR2B subunit abolishes the morphine induced place preference , whereas antibodies against the NR 1 and NR2A subunits do not affect the rewarding effect of morphine , indicating that the blockade of the NR2B subunit suppresses the development of the morphine induced rewarding effect . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have stably transfected HEK 293 cells with recombinant human CaBP in order to determine the influence of this protein upon transient increases in intracellular ionic calcium concentration ( [ Ca ( 2+ ) ] ( 1 ) ) induced either by transient transfection of the NR 1 and NR2A subunits of the N methyl D aspartate ( NMDA ) receptor and brief exposure to glutamate , photolysis of the caged calcium compound NP EGTA , or exposure to the Ca ( 2+ ) ] ionophore 4 Br A 23187 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Functional studies have also supported selective targeting of NR2A and NR2B to synaptic and extrasynaptic populations , respectively . ^^^ The results indicate that : ( 1 ) no single cassette of NR 1 is differentially enriched in the postsynaptic densities and ( 2 ) the NR2A and NR2B subunits are similarly enriched at the synapse . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
While NR2A , NR2B and NR2D are known to be phosphorylated on tyrosine residues , the exact sites have remained unidentified . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Following NMDA insult , the mRNA levels for NR 1 ( com ) , NR2A , NR2B and , to a lesser extent , the NR2C subunit were substantially reduced within 24 hr post treatment ( PT ) , and remained depressed for up to 48 hr . ^^^ Protein levels for NR 1 ( com ) , NR2A and NR2B were also monitored at select time points following NMDA insult . ^^^ By day 10 , the levels of protein expression for NR 1 ( com ) and NR2B remained suppressed despite the rise in gene expression for these two subunits , whereas protein for NR2A showed a substantial rise in expression . ^^^ Of the five genes assayed , NR2A and NR2B showed the greatest reduction in expression following NMDA treatment , suggesting that one or both of these subunit may signal events leading to neuronal cell death in the retina . ^^^ The preferential down regulation of the NR2A and NR2B receptor subunits may implicate these subunits as key players in mediating the excitotoxic signal in the retina and possibly elsewhere in the brain . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Whole cell voltage clamp recordings were performed to assess the ability of conantokin G ( con G ) , conantokin T ( con T ) , and a 17 residue truncated form of conantokin R ( con R [ 1 17 ] ) to inhibit N methyl d aspartate ( NMDA ) evoked currents in human embryonic kidney 293 cells transiently expressing various combinations of NR1a , NR1b , NR2A , and NR2B receptor subunits . ^^^ Con T and con R [ 1 17 ] attenuated ion currents in cells expressing NR1a / NR2A or NR1a / NR2B . ^^^ Con G did not affect NMDA evoked ionic currents in cells expressing NR1a / NR2A , but it showed inhibitory activity in cells expressing NR1a / NR2B receptors and the triheteromeric combination of NR1a / NR2A / NR2B . ^^^ A sarcosine containing con T truncation analog , con T [ 1 9 / G1Src / Q6G ] , inhibited currents in NR1a / NR2A and NR1a / NR2B receptors , eliminating residues 10 21 as mediators of the broad subunit selectivity of con T . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RESULTS : In situ hybridization of riboprobes indicated that , in brain sections from both WSP and WSR mice , there was a differential regional distribution of mRNA for the mouse NR 1 , NR2A , NR2B , and NR2C NMDA receptor subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Specific probes were designed for the different subunits of the NMDAR , and we showed by RT PCR analysis that mammalian osteoclasts expressed NR2B and NR2D subunits mRNAs but not NR2A and NR2C mRNAs . ^^^ Rat calvaria and MG 63 osteoblastic cells also expressed several NR 2 subunits mRNAs , namely NR2A , NR2B , and NR2D . ^^^ In situ hybridization on isolated rabbit osteoclasts and MG 63 cells has confirmed the localization of NR 1 , NR2B , and NR2D transcripts in osteoclasts and NR 1 , NR2A , NR2B , and NR2D transcripts in MG 63 cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Expression of NR2A and NR2B mRNAs was 40 50 % lower in PDC treated cells as compared to control . ^^^ Immunoblot experiments revealed that PDC exposure reduced the expression of NR 1 and all NR 2 proteins with NR2A and NR2B proteins being reduced to a greater extent than NR 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We found that the NMDA receptor ( NR ) subunits NR 1 , NR2A and NR2B were expressed in all three cultures . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 , NR2A , and NR2B mRNA levels were measured by RNase protection assay in young ( 3 4 month ) , middle aged ( 12 13 month ) , and aged ( 24 25 month ) Sprague Dawley rats in different phases of the estrous cycle in cycling animals and in acyclic subjects . ^^^ Age related changes were observed in NR 1 , NR2A , and NR2B in the ventral hippocampus and in NR 1 and NR2B in the dorsal hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
A crude synaptic membrane fraction ( SPM ) was prepared and assayed with antibodies specific for either the NR 1 , NR2A or NR2B subunits . ^^^ Relative to control animals raised in a diurnal light dark cycle , a period of 1 week of dark rearing caused an increase in the relative amount of NR 1 , a decrease in the level of NR2A , and no change in the level of NR2B subunit expression in postnatal day 12 rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We found that 3 days of treatment of cerebellar granule neurons with ethanol , which was previously reported to increase NMDA receptor function , resulted in a delay in the ' developmental switch ' of the NR2A and NR2B subunits , i . e . the developmental decrease in NR2B and increase in NR2A protein expression . ^^^ As a result , the level of NR2B was higher , and that of NR2A was lower , in the ethanol treated cells than in control cells . ^^^ Changes in the proportion of NR2A and NR2B subunits may contribute to effects of ethanol on neuronal development . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we show that in rat striatal tissues the NR2A and NR2B subunits in the synaptosomal membrane , and not those in the light membrane and synaptic vesicle enriched compartments , are tyrosine phosphorylated . ^^^ The dopamine D 1 receptor agonist SKF 82958 produces ( 1 ) an increase in NR 1 , NR2A , and NR2B proteins in the synaptosomal membrane fraction ; ( 2 ) a decrease in NR 1 , NR2A , and NR2B proteins in the light membrane and synaptic vesicle enriched fractions ; and ( 3 ) an increase in the tyrosine phosphorylation of NR2A and NR2B in the synaptosomal membrane compartment . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Therefore , the present study was designed to investigate the effects of short and long term estrogen replacement or deprivation on mRNA levels of three NMDA receptor subunits , NR 1 , NR2A , and NR2B , in the hippocampus of aging female Sprague Dawley rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tat increased 32P incorporation into NMDA receptor subunits NR2A and NR2B and this effect was blocked by genestein . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have investigated the tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B by exogenous Src and Fyn and compared this to phosphorylation by tyrosine kinases associated with the postsynaptic density ( PSD ) . ^^^ Src and Fyn phosphorylated similar sites in NR2A and NR2B , tryptic peptide mapping identifying seven and five major tyrosine phosphorylated peptides derived from NR2A and NR2B , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptor currents were measured utilizing the two electrode voltage clamp technique on Xenopus oocytes injected with NR 1 1a cRNA and either NR2A , NR2B or NR2C cRNA . ^^^ Efficacies of DCS were 35 68 % of glycine controls for channels containing NR 1 1a and NR2A or NR2B subunits , but channels containing NR2C subunits had efficacies greater than glycine controls ( 192 % ) . ^^^ The efficacy of DCS was pH sensitive for NR2A or NR2B containing channels , but not for channels containing NR2C . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In normal control animals , regional differences in the expression of several glutamate receptor subunit / subtypes , e . g . , NR 1 and NR2A subunits of the N methyl D aspartic acid ( NMDA ) receptor , GluR 2 and KA 2 subtypes of non NMDA receptors , and mGluR 1 and mGluR 7 metabotropic glutamate receptors , were consistent with previous results from studies using in situ hybridization histochemistry , suggesting that the real time quantitative PCR method was a reliable procedure for evaluation of changes in mRNA expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ribonuclease protection assays were carried out to determine the levels of NR2A , NR2B , and NR2C mRNA within the treatment groups determined . ^^^ Within the anti calnexin immunoprecipitates , no NR2A , NR2B or NR2C subunits were detectable , but a significant pool of NR 1 subunits was identified . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A and NR2B subunit mRNAs were expressed in the hippocampus and the cortex of both the control and the prenatal HI rat brains . ^^^ Intrauterine HI did not significantly affect expression of either the NR2A or NR2B subunit mRNA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The effects of continuous infusion of NMDA receptor antagonist MK 801 on the modulation of NMDA receptor subunits NR 1 , NR2A , NR2B , and NR2C were investigated by using in situ hybridization study . ^^^ Continuous intracerebroventricular ( i . c . v . ) infusion with MK 801 ( 1 pmol / 10 microl / h ) for 7 days resulted in significant modulations in the NR 1 , NR2A , and NR2B mRNA levels without producing stereotypic motor syndromes . ^^^ In contrast to NR2A , NR2B subunit mRNA levels were increased ( 10 14 % ) in the cerebral cortex , caudate putamen , and thalamus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we report that the fast desensitization of NR1 / NR2A receptors is caused by ambient zinc , and that a positive allosteric interaction occurs between the extracellular zinc binding site located in the amino terminal domain and the glutamate binding domain of NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ifenprodil ( 10 microM ) was used to distinguish between the NR2A and NR2B components of NMDA fEPSPs . ^^^ Ethanol inhibition of the CA 1 NR2B mediated component was five to seven times lower in NR2A ( DeltaC / DeltaC ) than in C57Bl / 6 . ^^^ The altered sensitivities to ethanol of both NR2A and NR2B mediated responses in the CA 1 of NR2A ( DeltaC / DeltaC ) imply that NR2A and NR2B subunit containing NMDA receptors may be linked by a common target of ethanol . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N methyl D aspartate receptors ( NRs ) are a group of ionotropic glutamate receptors in the brain and they are composed of heteromeric subunits ( NR 1 , NR2A D and NR 3 ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Based on immunoblot analysis , calpain cleavage of NR2A , NR2B and NR2C subunits was limited to their C terminal region . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This region in NR 1 and NR2B controls the action of NR2B selective drugs like ifenprodil , while this domain in receptors containing the NR2A subunit controls the action of NR2A selective drugs such as zinc . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We conclude that the synaptic ratio of NR2B over NR2A in the NMDA receptor complex in the visual cortex is not significantly influenced by the transgene overexpression . ^^^ These data suggest that mRNA availability is not a limiting factor for the synthesis of NR2B protein in the visual cortex , and support the hypothesis that levels of NR2A , rather than NR2B , normally determine the subunit composition of NMDARs in visual cortex . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of synaptic membranes by Src significantly reduced calpain mediated truncation of both NR2A and NR2B subunits of NMDA receptors , but not of GluR 1 subunits of AMPA receptors . ^^^ Similar results were observed with NR2A and NR2B C terminal domain fusion proteins phosphorylated by Fyn or Src before incubation with calpain and calcium . ^^^ In addition , phosphorylation of NR2A and NR2B C terminal fusion proteins by Fyn or Src enhanced their binding to spectrin and PSD 95 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of the NMDA receptor subunits NR2A and NR2B was also enhanced following SE . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This sequence is also present in the extracellular domain of murine and human NMDA ( N methyl D aspartate ) receptor subunits NR2a and NR2b . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Unexpectedly , we found that expression of NR 1 ( including the expression of NR 1 subunits containing the N 1 , C 1 , and C 2 inserts ) , NR2A , NR2B , NR2C , GluR 1 , GluR2 / 3 , GluR 5 , GluR6 / 7 , and KA 2 subunits was not altered in hippocampal homogenates from ethanol exposed rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
When coexpressed with NR 1 and NR2A in heterologous cells , NR3B suppressed glutamate induced current similarly to NR3A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Since dioxin and related compounds are suspected of affecting permanently the brain function of offspring of human and experimental animals , effects of perinatal exposure to 2 , 3 , 7 , 8 tetrachlorodibenzo p dioxin ( TCDD ) on the expression of rat NMDA receptor NR2A and NR2B subunit mRNA were examined . ^^^ The mRNA quantification by competitive RT PCR clearly revealed that TCDD inhibited NR2B mRNA expression and enhanced NR2A mRNA expression in the neocortex and hippocampus on postnatal day ( PND ) 49 , whereas these changes in mRNA expression were not found on PND 5 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have localized regions in the S 1 binding domain of both subunits required for the transmission of allosteric signals from the glutamate binding NR2A subunit to the glycine binding NR 1 subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Two electrode voltage clamp on Xenopus oocytes expressing NR 1 1a and either NR2A , NR2B or NR2C subunits yielded biphasic ACPC dose response curves with 15 microM NMDA . ^^^ NR 1 1a / NR2B and NR 1 1a / NR2C subunit combinations yielded overlapping dose response curves with a maximal efficacy of approximately 80 % ; the maximal efficacy of ACPC for the NR 1 1a / NR2A subunit combination was significantly lower at approximately 60 % . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Since the sites for tyrosine phosphorylation and nitration are on adjacent carbon atoms , the present study tested the hypothesis that in vitro dephosphorylation of the NR 1 , NR2A and NR2B subunits of the NMDA receptor increases receptor nitration by peroxynitrite in cortical membranes of newborn piglets . ^^^ Nitration was measured by immunoprecipitating with agarose conjugated anti nitrotyrosine antibody followed by Western blot analysis using specific anti NR 1 , NR2A and NR2B primary antibodies . ^^^ The data demonstrate that nitration of the NR 1 , NR2A and NR2B subunits of the NMDA receptor increases following dephosphorylation in both normoxic and hypoxic animals , however increase is much higher in hypoxic animals . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Parallel measurements of free glutamate and homocysteine levels in the patients blood plasma did not reveal any substantial changes of the given parameters , comparing to indices of autoantibodies to NR2a glutamate receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
They were designated as the NR 1 ( sigma 1 ) , NR2A ( epsilon 1 ) , NR2B ( epsilon 2 ) , NR2C ( epsilon 3 ) , and NR2D ( epsilon 4 ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This study was aimed to investigate gene expression of N methyl D aspartate ( NMDA ) receptor subunits ( NR 1 , NR2A , NR2B ) in rat adrenal gland under basal and stress conditions , using RT PCR . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
These effects of InsP 6 appear to be specific to AMPA receptors , as neither 3H glutamate binding to NMDA receptors nor levels of NR 1 and NR2A subunits in P 2 and P 3 fractions were affected . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The increase in the NR 1 , NR2A , and NR2B receptor mRNAs started at 5 h , maintained for 1 7 days ( P < 0 . 05 0 . 001 ) and returned to the control level at 14 days after inflammation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Nicotine exposure during a postnatal critical period alters NR2A and NR2B mRNA expression in rat auditory forebrain . ^^^ Since glutamatergic activity regulates levels of postsynaptic NMDAR subunits , here we examine the effects of CNE on mRNA expression for the NR2A and NR2B subunits in auditory cortex and thalamus . ^^^ Two days of CNE ( postnatal days 8 9 ) , produced no effects , but 5 days ( postnatal days 8 12 ) enhanced cortical NR2A mRNA levels and reduced thalamic NR2B mRNA levels for up to 2 weeks . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we describe the use of real time quantitative PCR using a fluorescent TaqMan probe , to study the regional differences in expression of glutamate receptor subunit / subtype genes ( NR 1 , NR2A , GluR 2 , KA 2 , mGluR 1 , mGluR 7 ) in the central vestibular system including the vestibular nucleus complex , inferior olive and cerebellar flocculus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Semi quantitative Western blot techniques were applied with the use of antibodies that recognize either the epsilon 1 ( NR2A ) , epsilon 2 ( NR2B ) or zeta 1 ( NR 1 ) subunits of the NMDA receptor or a synaptic terminal protein , synaptophysin . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The NMDA receptor subunits , NR 1 , NR2A , NR2B , NR2C , and NR2D have been cloned in several species , including man . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N methyl D aspartate receptors : transient loss of NR1 / NR2A / NR2B subunits after traumatic brain injury in a rodent model . ^^^ In this study , temporal changes in NMDA receptor subunit ( NR 1 , NR2A , and NR2B ) levels in rat hippocampus after TBI were investigated by Western blot and mRNA expression levels by RT PCR methods . ^^^ Western blot analysis revealed significant decreases of NR 1 , NR2A , and NR2B subunit proteins at 6 and 12 hr postinjury in rat hippocampus . ^^^ Complete recovery of NR 1 , NR2A , and NR2B subunit protein to the levels of sham controls was observed at 24 hr postinjury . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Differential NR2A and NR2B expression between trigeminal neurons during early postnatal development . ^^^ We conducted receptor immunohistochemistry studies to demonstrate the expression of NR2A and NR2B subunits in trigeminal motoneurons ( Mo 5 ) and mesencephalic trigeminal neurons ( Me 5 ) during the first 2 weeks of development . ^^^ In Mo 5 , NR2A and NR2B immunoreactivity was observed throughout the time frame sampled . ^^^ A significant increase in the NR2A : NR2B ratio occurred between P 3 4 and P 11 due to a reduction in the number of NR2B immunoreactive neurons . ^^^ The temporal and spatial expression of NR2A and NR2B was differentially regulated between caudal and rostral regions of Me 5 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We generated expression vectors for N terminally green fluorescent protein tagged NR2A and NR2B subunits ( GFP NR2A and GFP NR2B ) . ^^^ Fifteen days after transfection , well defined GFP clusters were observed for both GFP NR2A and GFP NR2B subunits being co localized with endogenous NR 1 subunit . ^^^ Live staining with anti GFP antibody demonstrated the surface expression of GFP NR2A and GFP NR2B subunits that was partly co localized a presynaptic marker . ^^^ Later in development , a large portion of surface clusters for both GFP NR2A and GFP NR2B subunits were clearly localized at dendritic spines . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast , no high affinity specific binding was detected to NR 1 , NR2A , NR2B subunits expressed alone or NR1 / NR2A receptors . ^^^ HEK 293 cells were transfected with NR 1 , NR2A and NR2B receptor subunits and complexes comprising all three subunits were isolated by anti NR2A immunoaffinity chromatography . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blotting analysis demonstrated that the expression of NR2A decreased 2 3 days after in vivo axotomy , while expression of NR 1 and NR2B , NR2C and NR2D subunits did not change significantly . ^^^ In conclusion , a decrease in NR2A expression results in the appearance of functional characteristics of the NMDA receptor predominantly containing the NR2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A , NR2B , and NR2D subunit transcripts were present in both nondifferentiated and neuronally differentiated cultures , while NR2C subunits were expressed only transiently , during the early period of neural differentiation . ^^^ NR 1 and NR2A subunit proteins were detected both in nondifferentiated progenitor cells and in neurons , while the mature form of NR2B subunit protein appeared only at the time of neuronal process elongation . ^^^ Despite the early presence of NR 1 and NR2A subunits , NMDA evoked responses could be detected in NE 4C neurons only after the sixth day of induction , coinciding in time with the expression of the mature NR2B subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Although early song deprivation ( which extends the sensitive period for song learning ) delays changes in NR2B gene expression and NMDAR physiology within the lMAN , it did not alter NR2A mRNA levels measured at PHD 40 , 45 , or 60 . ^^^ Early testosterone ( T ) treatment , which disrupts vocal development and accelerates the maturation of both NR2B levels and NMDAR physiology in lMAN , also significantly increased NR2A transcripts measured at PHD 35 in lMAN . ^^^ Together with our previous studies , these results show that in a pathway critical for vocal plasticity , the ratio of NR2A : NR2B mRNA rises abruptly early during the sensitive period for song learning . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blots indicated that rat dorsal root ganglia ( DRG ) contain NR 1 , NR2B , NR2C , and NR2D but not NR2A . ^^^ Real time RT PCR showed that NR2B and NR2D were expressed at higher levels than NR2A and NR2C in DRG . ^^^ Immunofluorescence with an antibody that recognized NR 1 and another that recognized NR2A and NR2B showed that NR 1 and NR2B colocalized in 90 % of DRG neurons , including most A fibers ( identified by the presence of neurofilament 200 kDa ) . ^^^ The percentage of cells immunoreactive for NR 1 , NR2A / NR2B , and NR2C / NR2D were the same in the T 9 , T 12 , L 4 , and L 6 DRG . ^^^ The intracellular distribution of the NR 2 subunits was strikingly different : Whereas NR2A / NR2B immunoreactivity was found in the Golgi apparatus and occasionally at the plasma membrane , NR2C / NR2D immunoreactivity was found in the cytoplasm but not in the Golgi . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Identification of a new site in the S 1 ligand binding region of the NMDA receptor NR2A subunit involved in receptor activation by glutamate . ^^^ To further define the amino acids through which glutamate activates the receptor , we generated single site mutations to the NR2A subunit , and expressed them with wild type NR 1 in HEK 293 cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In general , oestrogen had inhibitory effects on NR 1 and NR2a , and differential effects on NR2b subunit mRNA levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Trapping resulted in a significant reduction of NMDA receptor 1 ( NR 1 ) in each of the regions examined but did not affect the levels of either NMDA receptor 2A or B ( NR2A or NR2B ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Studies were conducted to evaluate the effects of s . c . injected recombinant human growth hormone ( GH ) on the expression of the gene transcript of N methyl D aspartate receptor subunits type 1 ( NR 1 ) , type 2A ( NR2A ) , and type 2B ( NR2B ) in the male rat hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To test this hypothesis the effect of hypoxia on the nitration of the NR 1 , NR2A and NR2B subunits of the NMDA receptor was determined . ^^^ There was a significant increase in the nitrated NR 1 , NR2A and NR2B subunits following hypoxia : 104+ / 11 vs . 275+ / 18 optical density ( OD ) xmm ( 2 ) for NR 1 ( P < 0 . 05 ) , 212+ / 36 vs . 421+ / 16 ODxmm ( 2 ) for NR2A ( P < 0 . 05 ) and 246+ / 44 vs . 360+ / 26 ODxmm ( 2 ) for NR2B ( P < 0 . 05 ) . ^^^ This increase in nitrated NR 1 , NR2A and NR2B subunits of the NMDA receptor was prevented by the administration of NNLA prior to hypoxia ( NR 1 160+ / 19 , P=NS , NNLA vs . normoxic ; NR2A 304+ / 49 , P=NS , NNLA vs . normoxic , and NR2B 274+ / 19 , P=NS , NNLA vs . normoxic ) . ^^^ The increase in nitration of the NR 1 , NR2A and NR2B subunits of the NMDA receptor increased as a function of decreased cerebral high energy phosphates , ATP and phosphocreatine , during hypoxia . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Protein levels of NR 1 , phosphorylated NR 1 897serine , NR2A and NR2B in striatum were measured by immunoblotting after piglets underwent hypoxic asphyxic cardiac arrest , cardiopulmonary resuscitation , and recovery for 3 , 6 , 12 or 24 h . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Their location in a modeled three dimensional structure suggests that ifenprodil binds in the cleft of the LIVBP like domain of NR2B by a mechanism ( Venus flytrap ) resembling that of the binding of Zn on the LIVBP like domain of NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N methyl D aspartate receptor subunit NR2A and NR2B messenger RNA levels are altered in the hippocampus and entorhinal cortex in Alzheimer ' s disease . ^^^ Quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) was used to determine the messenger RNA ( mRNA ) levels of the NMDA receptor subunits NR 1 , NR2A , and NR2B in the hippocampus and entorhinal cortex of postmortem brain samples from nine clinically well characterized AD patients and nine aged controls . ^^^ Reductions of NR2A ( 46 . 2 % , p < 0 . 01 ) and NR2B ( 43 . 2 % , p < 0 . 0001 ) mRNA levels were identified in the entorhinal cortex . ^^^ Reductions of NR2A ( 41 . 4 % , p < 0 . 05 ) and NR2B ( 40 . 6 % , p=0 . 058 ) mRNA levels were found in the hippocampus . ^^^ No significant changes of subunit NR2A and NR2B mRNA levels were identified in the cerebellum . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Sequence determinants on the NR2A and NR2B subunits of NMDA receptor responsible for specificity of phosphorylation by CaMKII . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To elucidate mechanisms controlling the number and subunit composition of synaptic NMDA Rs in hippocampal slice neurons , the NR 1 , NR2A , and NR2B subunits were optically and electrophysiologically tagged . ^^^ The NR 2 subunit directs delivery of receptors to synapses with different rules controlling NR2A and NR2B . ^^^ NR2A containing receptors whose expression normally increases with age replace synaptic NR2B containing receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ro 63 1908 selectively blocked recombinant receptors expressed in Xenopus oocytes containing NR1C + NR2B subunits with an IC ( 50 ) of 0 . 003 microM and those containing NR1C + NR2A subunits with an IC ( 50 ) of > 100 microM , thus demonstrating greater than 20 , 000 fold selectivity for the recombinant receptors expressing NR1C + NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 was co associated with NR2A , NR2B and NR2D but not NR2C , nor was NR2C detected in adult mouse hippocampal membranes . ^^^ NR2A and NR2B like immunoreactivity essentially co localised with that of NR 1 implying co assembly of all three subunits in this brain structure . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Colocalization of the three NMDAR subunits that are most abundant in the hypothalamus ( NR 1 , NR2A , or NR2B ) with GnRH perikarya was determined in female rats at different stages of the reproductive life cycle : young ( 3 4 months ) rats with regular estrous cycles , middle aged ( 8 10 months ) rats with regular estrous cycles , middle aged rats with irregular estrous cycles , and middle aged acyclic rats in persistent estrus . ^^^ The number , percent , and localization of GnRH perikarya expressing NR 1 , NR2A , or NR2B were mapped and quantified by double label immunofluorescence microscopy . ^^^ This may result in an age related increase in the ratio of the NR2B to the NR 1 and NR2A subunits on GnRH neurons . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RESULTS : There was an increase in NR2A and NR2B concentration in DY group compared to all other treatments . ^^^ CONCLUSIONS : NR2A and NR2B receptor concentrations in hippocampus of the rats maintained in dark showed significant increases compared to the control and functional pinealectomy groups but there was no significant increase in lipid peroxidation . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Furthermore , zinc exposure increased levels of total and tyrosine phosphorylated forms of NR2A and NR2B in a PP 2 dependent manner in both hippocampal slices and cell cultures . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptors are ionotropic glutamate receptors assembled of subunits of the NR 1 and of the NR 2 family ( NR2A NR2D ) . ^^^ As an overall result of studies on recombinant and native NMDA receptors , ethanol inhibits the function of receptors containing the subunits NR2A and / or NR2B to a greater extent than those containing NR2C or NR2D . ^^^ For example , in rat cultured mesencephalic neurons , NR2C expression was developmentally increased , whereas expression of NR2A and NR2B was decreased . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The patterns of CaMKII and NR1 / NR2A were better correlated than were CaMKII and NR2B , or CaMKII and GluR 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
METHODS : N methyl D aspartate gated currents were measured using whole cell voltage clamp recording in cortical neurons cultured for 1 4 weeks and HEK 293 cells transiently expressing NR 1 1a + NR2A or NR 1 1a + NR2B subunit containing receptors . ^^^ NMDA gated currents mediated by NR2A containing receptors were less sensitive to isoflurane than those mediated by NR2B containing receptors . ^^^ This effect of isoflurane was subunit dependent with the NR2B containing receptors more sensitive to isoflurane than the NR2A containing receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Electrophysiological experiments revealed that homoquinolinate activated human recombinant NR1a / NR2A , NR1a / NR2B and NR1a / NR2A / NR2B receptors with EC 50 values of 25 . 2 , 13 . 8 and 9 . 04 micro m , respectively , with intrinsic activities of 148 , 93 . 3 and 125 % , respectively , compared to glutamate ( =100 % ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A levels in the right hemisphere of P 7 pups were markedly reduced after 24 h of recovery , while NR 1 and NR2B remained unchanged . ^^^ In contrast , NR2B , but not NR2A , was reduced after HI at P 21 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Regarding the NR 2 subunit expression in controls , NR2B exhibited the highest expression , followed by decreasing proportions of NR2C , NR2A , and NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the present paper , we investigated whether the expression of NMDAR subunits NR 1 , NR2A , NR2B is influenced by neurotrophins in rat visual cortex . ^^^ Different antibodies were used to stain neurons expressing NR 1 , NR2A and NR2B . ^^^ We counted the number of neurons stained for NR2A and NR2B subunits and expressed this as percentage with respect to the total number of cresyl violet stained neurons in each cortical layer . ^^^ The staining level for both NR2A and NR2B , but not NR 1 , was reduced in all cortical layers in TrkB IgG treated animals . ^^^ In agreement with this result , the endogenous levels of NR2A and NR2B subunits were reduced in TrkB IgG treated animals as shown by Western blotting . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Overexpression of either NR2A or NR2B , but not splice variants of NR 1 , by transfection caused a significant increase in the total number of functional NMDA receptors and in surface NR 1 subunit cluster density in CGCs in primary culture . ^^^ Overexpression of either NR2A or NR2B significantly altered the deactivation kinetics of NMDA mediated miniature EPSCs ( NMDA mEPSCs ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using the anti NR3A antibody in immunoprecipitation studies , we find that 9 . 7 + / 0 . 8 % of NR 1 , 8 . 7 + / 1 . 8 % of NR2A , and 5 . 0 + / 0 . 6 % of NR2B are associated with NR3A at postnatal day 10 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Two regions in the D 1 receptor carboxyl tail can directly and selectively couple to NMDA glutamate receptor subunits NR 1 1a and NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
A 30 % reduction in alpha amino 3 hydroxy 5 methyl 4 iso xazolepropionate ( AMPA ) GluR 1 and 2 subunit immunoreactivities was observed in the preconditioned cortex ( p < 0 . 03 ) , but there was no significant change in the NMDA receptor subunits , NR 1 , NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analyses of cell homogenates indicated upregulation of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) , but downregulation of synaptic organizing proteins ( PSD 95 , densin 180 , and septin 6 homologue ) and a synapse enriched enzyme ( alphaCaMKII ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We have shown that an Src family kinase Fyn phosphorylates NR2A and NR2B subunits of the NMDA receptor . ^^^ We have identified tyrosine phosphorylation sites on NR2A and NR2B . ^^^ A phosphorylation of the other tyrosine residues of NR2A and NR2B would also be involved in brain development and function . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Delta catenin forms stable complexes with excitatory neurotransmitter receptors including ionotropic N methyl D aspartic acid receptor 2A ( NR2A ) , metabotropic glutamate receptor 1alpha ( mGluR1alpha ) , as well as PSD 95 in vivo . ^^^ Upon glutamate receptor activation , delta catenin becomes down regulated and its association with NR2A and mGluR1alpha in cultured neurons is diminished . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Although there was no apparent difference in the actions of light between wild type NR 1 NR2A and NR 1 NR2B subunit configurations , the light enhancement of NMDA induced currents was either completely abolished or substantially diminished in the redox site mutants NR1a ( C744A , C798A ) NR2B and NR1a ( C744A , C798A ) NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We investigated the effects of transient ( 15 min ) global ischemia on tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B , and the interaction of NR 2 subunits with the SH 2 domain of phosphatidylinositol 3 kinase ( PI 3 kinase ) in vulnerable CA 1 and resistant CA3 / dentate gyrus of the hippocampus . ^^^ The SH 2 domain of the p 85 subunit of PI 3 kinase bound to NR2B , but not to NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Postnatal development of NR2A and NR2B mRNA expression in rat auditory cortex and thalamus . ^^^ Sensory cortex in the rat undergoes rapid postnatal development , especially following the onset of sensory function during so called `` critical periods . ' ' To investigate potential mechanisms in the auditory forebrain involving different NMDA receptor subunits , we have used in situ hybridization to determine expression patterns of NR2A and NR2B mRNA at postnatal days 4 , 10 , 13 , 18 , 25 , and adult . ^^^ Initially , NR2A mRNA expression is very low , whereas NR2B mRNA expression is moderate ; both levels increase over approximately 2 weeks . ^^^ Among medial geniculate subdivisions , NR2A mRNA expression occurs preferentially in the medial division , whereas NR2B mRNA expression is strongest in the ventral division . ^^^ For auditory cortex and thalamus , NR2A and NR2B mRNA expression peaks about 1 week after the onset of hearing before declining slightly into adulthood . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ethanol decreased the effect of NMDA similarly in all brain areas tested and had similar effects on Chinese hamster ovary cells expressing NR2A or NR2B subunits with an NR 1 1a subunit . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Real time PCR data revealed that striatal NOS interneurons express the mRNAs encoding NR 1 , NR2A , NR2B , and NR2D but not NR2C . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In this study , we examined the antinociceptive and motor effects of cone snail venom derived peptides , conantokins G and T ( conG and conT ) , which are selective inhibitors of the NR2B or NR2A and NR2B subtypes of the NMDA receptor , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Interestingly , the decrease in the number of synaptic NR2B subunits is followed by a parallel increase in the number of NR2A subunits at synapses . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Although NR2B subunit expression is uniform throughout striatum , NR2A subunit expression is greater laterally . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In situ hybridization histochemistry was used to determine the mRNA levels of the NMDA receptor subunits NR 1 , NR2A , NR2B , the AMPA receptor subunit GluR 1 , and the kainate receptor subunit KA 2 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In support of this hypothesis , we found that tyrosine phosphorylation of both NR2A and NR2B subunits was significantly reduced following in situ exposure of hippocampal slices to 100 mm ethanol . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The N methyl d aspartate ( NMDA ) glutamate receptor ( NMDAR ) , long implicated in developmental plasticity , shows decay time kinetics that shorten postnatally as NR2A subunits are added to the receptor . ^^^ Age matched WT cells showed NR2A rich receptors predominating in minis , yet slow NR2B mediated currents persisted in evoked currents . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Six hours after eye opening , increased dendritic PSD 95 coimmunoprecipitates with the same proportions of stargazin , increased proportions of the N methyl d aspartate receptor subunit NR2A , and decreased proportions of NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Transcript levels for the constitutive NMDAR subunit ( NR 1 ) and two modulatory subunits ( NR2A , NR2B ) were measured in four song control nuclei : lMAN , Area 10 , HVc , and RA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Overexpression of the NMDA receptor NR2A subunit , but not the NR2B subunit , prevented NMDA mEPSC amplitude reduction when cotransfected with PSD 95gfp . ^^^ Surface staining of the epitope tagged NR 2 subunits revealed that colocalization with PSD 95gfp was higher for flag tagged NR2A subunit clusters than for flag tagged NR2B subunit clusters . ^^^ These data suggest that PSD 95 overexpression in CGCs favours synaptic maturation by allowing synaptic insertion of NR2A and depressing expression of NR2B subunits . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Prominent NMDA receptor mediated currents were recorded in PC 12 cells to which NR2A or NR2B cDNA was delivered without NR 1 cDNA . ^^^ The amplitudes of these responses were similar to those in PC 12 cells to which NR 1 cDNA was delivered together with NR2A or NR2B cDNA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 , NR2A and NR2B are the major NMDA receptor subunits expressed during development . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In situ hybridization was performed for additional gene expression studies of the NR 1 , NR2A , NR2B , NR2C , and NR2D subunits . [ 3H ] MK 801 binding was found to be increased after haloperidol treatment in the striatum and nucleus accumbens . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blot analysis of hippocampal postsynaptic densities ( PSD ) revealed a reduction in NR2B subunit protein , whereas the abundance of the other major N methyl D aspartate ( NMDA ) receptor subunits ( NR 1 , NR2A ) , AMPA receptor subunits ( GluR2 / 3 ) , scaffolding proteins , and Ca2+ / calmodulin dependent protein kinase 2 ( alphaCaMKII ) were unaltered . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using antibodies against NR 1 , NR2A , NR2B , NR2D , GluR 2 , 3 , and GluR 5 , 6 , 7 receptor subunits we demonstrated a pronounced disturbance of cortical immunostaining pattern in the cortical malformation . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Spatial learning induced phosphorylation of TrkB , Fyn , and NR2B , but not NR2A , in the hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
After 3 weeks of running , mRNA levels coding for NR2A and NR2B subunits were increased in the ventral tegmental area , while only NR2A mRNA levels were found to be elevated in the frontal cortex . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To test whether NMDARs prevalent in neonates ( NR 1 plus NR2B or NR2D subunits ) are inhibited by hypoxia compared with those in mature neurons ( NR2A and NR2C ) , we expressed these receptors in Xenopus oocytes . ^^^ Compared with responses in 21 % O ( 2 ) , hypoxia ( PO ( 2 ) 17 mm Hg ) reduced currents from neonatal type NR1 / NR2D receptors by 25 % , increased currents from NR1 / NR2C by 18 % , and had no effect on NR1 / NR2A or NR1 / NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The NR 1 gene deletion had no apparent effect on the levels of NR2A or NR2B mRNA but led to severe reductions of NR2A and NR2B protein in dendrites of CA 1 pyramidal cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of the NR2A and NR2B subunits of the N methyl d aspartate ( NMDA ) receptor by Src protein tyrosine kinases modulates receptor channel activity and is necessary for the induction of long term potentiation ( LTP ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Recent studies have provided evidence for the existence of both binary ( containing NR 1 and either NR2A or NR2B ) and ternary ( containing NR 1 , NR2A , and NR2B ) receptor complexes in the adult mammalian brain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Synapses in the central nucleus activate NMDA receptors that contain NR 1 and NR2B subunits , whereas synapses in the lateral nucleus contain receptors with both NR2A and NR2B subunits . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A but not NR2B N methyl D aspartate receptor subunit is altered in the visual cortex of BDNF knock out mice . 1 . ^^^ Aim of the present paper is to study the expression of N Methyl D Aspartate receptor ( NMDAR ) subunits NR2A and NR2B within mouse visual cortex . 2 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
N Methyl d aspartate ( NMDA ) receptor subunit 2A ( NR2A ) is an important modulatory component of the NMDA subtype of glutamate receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Mutations in NR2A confirmed that the analogous region controls the pH sensitivity of this subunit and also identified the linker region between the third transmembrane domain ( M 4 ) and the S 2 portion of the NR 2 glutamate binding domain as an additional determinant of proton sensitivity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
All neurones studied appeared to express the N methyl D aspartate ( NMDA ) receptor subunit NR 1 , and while antibodies recognising the NR2A and NR2B splice variants gave strong labelling , immunoreactivity with a NR2B specific antibody was weaker . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The majority of N methyl D aspartate receptors ( NMDA R ) in the adult forebrain are di or triheteromers composed of NR 1 , NR2A and NR2B subunits . ^^^ Dextromethorphan ( weakly NR2A selective ) ( 10 and 30 mg / kg , i . p . ) and ifenprodil ( highly NR2B selective ) ( 1 , 3 and 5 mg / kg , i . p . ) were tested in the GAT . ^^^ Since the selective blockade of the NR2B subunit by ifenprodil had no clear anxiolytic effect , the anxiolytic potential of NMDA subunit non selective agents might involve NR2A containing receptors . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Stress and stress hormones alter the expression of mRNA for the NR 1 , NR2A and NR2B subunits of the N methyl D aspartate ( NMDA ) receptor in brain regions associated with the stress response . ^^^ Using in situ hybridization histochemistry , we measured NR 1 , NR2A and NR2B mRNA expression in the hippocampus and in the hypothalamic paraventricular nucleus ( PVN ) of rats that had undergone adrenalectomy ( ADX ) or sham surgery before or after puberty . ^^^ Only prepubertal rats had an increase in dentate gyrus NR2A mRNA and CA 3 region NR2B mRNA following high dose replacement . ^^^ These results provide evidence that glucocorticoids have differential effects on the regional expression of mRNA NMDA receptor subunits and elucidate a window during adolescence in which the NR 1 , NR2A and NR2B genes are responsive to glucocorticoids . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we describe the laminar distribution of the cells expressing the NMDAR subunits NR2A and NR2B in the rat primary visual cortex at postnatal day ( P ) 21 and 37 using anti NR2A and anti NR2B antibodies and a stereological method to count labelled neurons . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using preembedding and postembedding immunostaining , as well as electron microscopy , we quantified the volumetric densities of NR 1 , NR2A , and NR2B containing synapses in layers 4 and 2 / 3 of the ferret visual cortex at different postnatal ages . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The distributions of NR2A and NR2C showed temporal and spatial similarities to that of NR 1 , while the expression of NR2B showed differences in the intensity and distribution . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Glutamate receptors AMPA GluR2 / 3 and NMDA R2A : NR2A ( NMDAepsilon 1 ) , and GABA ( A ) ( GABA ( A ) Ralpha 1 ) receptors were examined by immunohistochemistry in the cerebral cortex ( frontal cortex ) entorhinal cortex , hippocampus and cerebellar cortex in nine patients with sporadic Creutzfeldt Jakob disease ( CJD ) and eight age matched controls obtained 3 8 h after death . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Our results indicate that clozapine inhibits the NMDA receptor , but that this inhibition is subunit specific , being significantly greater for receptors containing NR2A and NR2B than for those containing NR2C . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
For this purpose , the effect of OTA on N methyl D aspartate ( NMDA ) receptor subunits 2A ( NR2A ) and 2B ( NR2B ) in the hippocampus and the protective effect of melatonin were investigated . ^^^ The concentrations of NR2A and NR2B in the OTA group were significantly lower than in the control group . ^^^ Melatonin exhibited a partially protective effect on NR2A and NR2B against OTA . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In visual cortex , NMDA receptor ( NMDAR ) properties depend primarily on NR2A and NR2B subunits , and NR 2 subunit composition changes with age and visual experience . ^^^ Experiments using NR2B selective and NR 2 subunit nonselective NMDAR antagonists demonstrated that NR2A and NR2B containing NMDARs contribute selectively to inhibitory LTD field LTP and excitatory LTP , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We report here that postsynaptic density 93 protein ( PSD 93 ) , a postsynaptic neuronal MAGUK , is expressed abundantly in spinal dorsal horn and forebrain , where it colocalizes and interacts with NMDAR subunits NR2A and NR2B . ^^^ Targeted disruption of the PSD 93 gene reduces not only surface NR2A and NR2B expression but also NMDAR mediated excitatory postsynaptic currents and potentials , without affecting surface AMPA receptor expression or its synaptic function , in the regions mentioned above . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In central neurons , NMDA receptors at immature glutamatergic synapses contain NR2B subunits and are largely replaced by NR2A subunits with development . ^^^ At mature synapses , NMDA receptors are thought to be multimers that contain either NR1 / NR2A or NR1 / NR2A / NR2B subunits , whereas receptors that contain only NR1 / NR2B subunits are extrasynaptic . ^^^ In contrast , at synapses on pyramidal neurons in the lateral amygdala , whereas NMDA EPSCs at immature synapses are slow and blocked by NR2B selective antagonists , at mature synapses their kinetics are faster and markedly less sensitive to NR2B selective antagonists , consistent with a change from NR2B to NR2A subunits . ^^^ Using real time PCR and Western blotting , we show that in adults the ratio of levels of NR2B to NR2A subunits is greater in the central amygdala than in the lateral amygdala . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Thus spontaneous NMDA receptor mediated events in DGGC are predominantly mediated by NR2A or possibly NR2A / NR2B receptors while the activation of NR2B receptors reduces the excitability of entorhinal afferents either directly or through an effect on the entorhinal cells . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the cerebral cortex of flurothyl treated rats , the protein expressions of NR 1 , NR2A , and NR2B decreased significantly , but NR2C increased significantly . ^^^ In the hippocampus of flurothyltreated rats , the protein expression of NR2A decreased significantly , the protein expression of NR2C increased significantly , and NR 1 and NR2B were similar to that in the hippocampus of control group . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Expression of NR2A and NR2B receptor mRNAs up regulated in cortex after 3 h of reperfusion following middle cerebral artery occlusion ( MCAo ) . ^^^ This effect was accompanied by an increase in NR2A and NR2B immunoreactivity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
These findings point to yet unknown , NMDA receptor activity dependent mechanisms that separately regulate the localization of NR2A and NR2B subunits at synapses . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Following ischemia , tyrosine phosphorylation of NR2A and NR2B and activated Src family kinases ( SFKs ) and Pyk 2 were increased in post synaptic densities ( PSDs ) . ^^^ Intravenous injection of GF immediately following the ischemic challenge resulted in decreased phosphorylation of NR 1 on PKC phosphorylation sites and reduced ischemia induced increases in tyrosine phosphorylation of NR2A and NR2B without affecting the increase in total tyrosine phosphorylation of hippocampal proteins . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Expression of NMDA receptor NR 1 , NR2A and NR2B subunit mRNAs during development of the human hippocampal formation . ^^^ We used in situ hybridization to examine the distribution and relative abundance of NR 1 , NR2A and NR2B subunit messenger ribonucleic acids in the hippocampal formation and adjacent cortex of 34 human subjects at five stages of life ( neonate , infant , adolescent , young adult and adult ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Substitution for PCP , disruption of prepulse inhibition and hyperactivity induced by N methyl D aspartate receptor antagonists : preferential involvement of the NR2B rather than NR2A subunit . ^^^ In conclusion , it appears that the NMDA receptor containing the NR2B , rather than the NR2A subunit , may play a major role in the PCP like discriminative stimulus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We tested whether synaptic transmission or plasticity was affected by acute dissociation of the PSD 95 NMDA receptor interaction with various peptides that bound to the first two PDZ domains of PSD 95 and its homologs and with antibodies directed against the very C terminus of the NR2A and NR2B subunits of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Most GAD , presumed projection neurons expressed both NR2A and NR2B mRNAs , and this profile did not change during chronic ethanol exposure . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Laminar distribution of NMDA receptor subunit ( NR 1 , NR2A , NR2B ) expression during the critical period in cat visual cortex . ^^^ The present study used immunohistochemistry to determine developmental changes in the laminar distribution of the three major cortical NMDA subunits ( NR2A , NR2B , NR 1 ) during the critical period in cat visual cortex . ^^^ For NR2A and NR2B , immunoreactivity in layer 4 remained low until 10 weeks of age . ^^^ At 20 weeks and adult , layer 4 filled in and NR2A and NR2B label was rather uniform across all layers . ^^^ NR 1 showed a developmental pattern of expression different from NR2A and NR2B after 1 week . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Based on differences in voltage dependence , decay kinetics , apparent Mg2+sensitivity , and subunit specific ( NR2A , NR2B , and NR2C / D ) pharmacology , we found NMDARs at these inputs to be distinct . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Under physiological conditions , PSD 95 , nNOS , NR2A , and NR2B were unaltered in the ( / ) pups . ^^^ However , at 24 hours after HI , protein expression of PSD 95 , nNOS , and NR2A but not NR2B was markedly higher in the ( / ) than in the ( + / + ) pups . ^^^ In ( + / + ) pups , HI resulted in decreased expression of NR2A but not NR2B in cortex and decreased NR2A and NR2B expression in hippocampus , but this reduction was not observed in ( / ) pups . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In addition , Western blots show a reduction in phosphorylated NR 1 , NR2A , and NR2B subunit protein expression in amygdalas from fear conditioned animals . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we show that NR2B , but not NR2A or NR 1 subunits of the NMDAR , interacts in vivo and in vitro with RasGRF 1 , a Ca ( 2+ ) / calmodulin dependent Ras guanine nucleotide releasing factor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Proteins were transblotted and probed with NMDA receptor subunit 1 ( NR 1 ) , NR2A or NR2B antibodies . ^^^ As tissue PCr levels decreased from 3 . 5 to 0 . 5 micromol / g brain during hypoxia , NR 1 , NR2A and NR2B protein increased by 84 , 56 and 38 % , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Cultures treated for up to 5 weeks with epidermal growth factor , fibroblast growth factor 2 , retinoic acid , and nerve growth factor displayed neuron like cellular processes and expressed neuronal markers , including the neuronal nuclear antigen NeuN , microtubule associated protein 2 , tau , synaptophysin , alpha ( 1A ) and alpha ( 1B ) calcium channel subunits , NR2A glutamate receptor subunits , and gamma aminobutyric acid . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At hippocampal CA 3 to CA 1 synapses of adult mice , different NMDAR subtypes with distinct functionality assemble from NR 1 with NR2A and / or NR2B subunits . ^^^ Similarly , NR2B specific antagonists reduced LTP in young but only marginally in adult wild type mice , further demonstrating that in mature CA 3 to CA 1 connections LTP induction results primarily from NR2A type signaling . ^^^ This finding is also supported by gene targeted mutant mice expressing C terminally truncated NR2A subunits , which participate in synaptic NMDAR channel formation and Ca2+ signaling , as indicated by immunopurified synaptic receptors , postembedding immunogold labeling , and spinous Ca2+ transients in the presence of NR2B blockers . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Although examining NMDA receptor subunit proteins showed no change in NR 1 , NR2A , or NR2B in the striata of the symptomatic mice , we observed a decrease in phosphorylation of NR 1 at Ser 897 , previously reported to decrease NMDA receptor current . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In striatal neurons , NR 1 , NR2A and NR2B mRNAs and immunoreactivity , and NR2D mRNA were found and the maximal levels of NR 1 mRNA and immunoreactivity expression were found at 6 day in vitro ( DIV ) . ^^^ NMDA receptors found at this stage in striatal neurons are likely to contain NR 1 plus NR2A , NR2B and NR2D subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Subtype specific antibodies demonstrated that the NR2B subunit was cleaved in these primary cultures , but the NR2A subunit was not . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
R6 / 2 mice had decreased levels of mRNAs encoding epsilon 1 and epsilon 2 NMDA receptor subunits ( mouse orthologs of rat NR2A and NR2B subunits ) , but not the zeta 1 subunit ( mouse ortholog of NR 1 ) , as assessed by gene expression profiling and Northern blotting . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Neuronal injury and expression of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) mRNA were detected by using in situ staining and RT PCR , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Activity dependent changes in expression and localization of the largest major isoform of the neural cell adhesion molecule NCAM 180 and three subtypes of glutamate receptors predominantly expressed in the outer part of the molecular layer of the dentate gyrus of adult rats the NMDA receptor NR2A , the AMPA receptor GluR2 / 3 , and the metabotropic glutamate receptor mGluR 1 were investigated using postembedding immunogold labeling , and electron microscopy . ^^^ Thus , changes in synaptic strength are associated with concomitant changes in the expression and distribution of NCAM 180 and glutamate receptors , particularly of the NR2A subtype . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Replacement of the biphenyl group of PBPD with a 9 oxofluorene ring system resulted in small changes in receptor affinity and subtype selectivity . 2 ' Bromo substitution on the biphenyl group of PBPD reduced antagonist affinity 3 to 5 fold at NR2A , NR2B and NR2D containing receptors , but had little effect on NR2C containing receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In striatal tissue from DARPP 32 depleted mice , basal tyrosine and serine phosphorylation of striatal NMDA receptor subunits NR 1 , NR2A , and NR2B was normal , and activation of dopamine D 1 receptors with the agonist SKF 82958 [ ( + / ) 6 chloro 7 , 8 dihydroxy 3 allyl 1 phenyl 2 , 3 , 4 , 5 tetra hydro 1H benzazepine ] produced redistribution of NMDA receptors from vesicular compartments ( P 3 and LP 2 ) to synaptosomal membranes ( LP 1 ) . ^^^ In the Fyn knockout mice , basal tyrosine phosphorylation of NR2A and NR2B was drastically reduced , whereas serine phosphorylation of these NMDA subunits was unchanged . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the absence of extracellular calcium , substitution of Arg for Asn 598 ( NR1R ) slowed desensitization by 2 to 3 fold compared with wild type NR1 / NR2A , and glutamate evoked peak current EC 50 and deactivation rate were also affected . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
A series of cysteine > alanine NR 1 2a mutants was generated , and the NR 1 2a mutants were co expressed with NR2A or NR2B subunits in mammalian cells and characterized with respect to cell surface expression , cell cytotoxicity post transfection , co association by immunoprecipitation , and immunoblotting following SDS PAGE under both reducing and non reducing conditions . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Rule learning regulates the composition of synaptic NMDARs in the piriform cortex , resulting in receptors with a higher complement of the NR2a subunit protein relative to NR2b . ^^^ As NR2a containing NMDARs mediate shorter excitatory postsynaptic currents than those containing NR2b , we suggest that learning induced regulation of NMDAR composition constrains subsequent synaptic plasticity , thereby maintaining the memory encoded by experience . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Thus , we investigated the expression of the NR 1 , NR2A , and NR2B subunits after combined prenatal and postnatal exposure to EtOH . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using quantitative Western blotting , the levels of NR 1 , NR2A and NR2B subunits were measured in the cortex and hippocampus of rats at different times after PTZ injection . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This study examined the effects of streptozotocin diabetes and insulin or gliclazide treatment on the hippocampal NMDA receptor subunit 2A and 2B ( NR2A and NR2B ) concentrations . ^^^ Eight weeks after the induction of diabetes MDA , levels were increased , and NR2A and NR2B concentrations were reduced . ^^^ Insulin and gliclazide treatment partially prevented the reduction of NR2A and NR2B expression and prevented the elevation of MDA levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We show here that an increase in NR2A subunit mRNA and a decrease in NR2B subunit mRNA within the vocal motor pathway accompany song learning in zebra finches ; however , manipulations that can alter the timing of song learning did not alter the course of these developmental changes . ^^^ We report that NR2A and NR2B mRNA levels did not change within the anterior forebrain or vocal motor pathways after adult deafening , even after substantial changes in song structure . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Pharmacological and genetic evidence indicates that combined inhibition of NR2A and NR2B subunit containing NMDA receptors is required to disrupt prepulse inhibition . ^^^ NR2A and NR2B , on sensorimotor gating in mice . ^^^ METHODS : These experiments utilised the non subtype selective NMDA antagonist dizocilpine , a line of NR2A KO mice and the selective NR2B antagonist Ro 63 1908 , in the study of pre pulse inhibition of the startle response ( PPI ) . ^^^ Conversely , selective genetic or pharmacological inhibition of either the NMDA NR2A or NR2B receptor subunit containing receptors , respectively , had no effect on PPI . ^^^ However , selective inhibition of NR2A and NR2B by administration of Ro 63 1908 to NR2A KO mice significantly disrupted PPI . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
For the inferior group , expression of NR2A and NR2B was positively correlated with place learning and probe trial performance in the water maze . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunocytochemical analysis confirmed expression of NR 1 , NR2A , and NR2B subunits in neurospheres cultured for 4 12 DIV . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Therefore , the present study was designed to investigate the effect of estrogen on the synaptic and subsynaptic distributions of the NMDA receptor subunits , NR2A and NR2B in CA 1 pyramidal cells , within the context of aging . ^^^ Our results demonstrated that the overall synaptic levels of NR2A and NR2B are similar in young and aged female rats , regardless of estrogen treatment . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The levels of cortical NR 1 NR2A and NR2B subunit proteins were detected by immunoblotting . ^^^ For the 50 mg / kg group , both the NR2A and NR2B subunits proteins increased at 1 h in the cortex and then decreased ; the protein levels returned to normal after 48 h . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In recent papers , alterations in subunit composition of NMDA receptors were reported after long term ethanol exposure . mRNA and / or protein levels of NR2A and NR2B types of subunits were found elevated both by in vivo and in vitro experiments . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This article focuses on NMDA receptor subunit changes that occur in the forebrain and midbrain during development , namely the switch from predominance of NMDA receptors rich in NR2B subunits to that of NMDA receptors rich in NR2A subunits . ^^^ In this model , different NMDA receptor scaffolding and signaling complexes effect the trafficking and synaptic localization of NR2A rich and NR2B rich receptors , leading to tangential compartmentalization of these receptors and their movement between synaptic and extrasynaptic compartments . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This included relatively lower levels of all four AMPA subunits in lMAN , strikingly higher levels of the kainite subunit GluR 5 in the robust nucleus of the arcopallium ( RA ) , higher and lower levels respectively of the NMDA subunits NR2A and NR2B in most vocal nuclei and lower levels of the metabotropic group 1 subtypes ( mGluR 1 and 5 ) in most vocal nuclei and the group 2 subtype ( mGluR 2 ) , showing a unique expression pattern of very low levels in RA and very high levels in HVC . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Taken together with our recent molecular studies , our data are consistent with a shift away from NMDA receptor subunit ( NR ) NR2B and 2C function toward increased NR2A subunit expression or function after chronic morphine , a process that could alter excitability and integrative properties and may represent a neuroadaptation of NAcc medium spiny neurons underlying morphine dependence . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Acutely after injury significant decreases were observed in the GluR 1 , GluR 2 , and GluR 4 subunits of the alpha amino 3 hydroxy 5 methyl 4 isoxazole proprionate ( AMPA ) receptor , and the NR2A and NR2B subunits , but not the NR 1 subunit , of the N methyl d aspartate ( NMDA ) receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Significantly lower expression of NR2A and NR2B transcripts was found in susceptible regions of AD brain , whereas expression of NR2C and NR2D transcripts did not differ from that in controls . ^^^ Western blot analysis confirmed a lower expression of the NR2A and NR2B isoforms at the protein level . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In this study , we identify a conserved family of membrane proximal endocytic signals , two within the NMDAR type 1 ( NR 1 ) subunit and one within the NR2A and NR2B subunits , necessary and sufficient to drive the internalization of NMDARs . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Environmental enrichment reversed the effects of maternal care on synaptic density and this effect was , in turn , associated with a reversal of the effect of maternal care on the NR2A and NR2B subunits of the NMDA receptor , as well as effects on ( RS ) alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid ( AMPA ) receptor subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Compared with the control rats , the protein expressions of NR 1 , NR2A and NR2B in the cerebral cortex and NR2A in the hippocampus of the recurrent seizure group was significantly decreased , but NR2C protein expression in the cerebral cortex and hippocampus significantly increased . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The aim of this study was to investigate whether L Asp and NMDA inhibit histamine stimulated GMBF and to examine the expression patterns of NMDA receptor subunits NR 1 , NR2A , and NR2B in rat stomach . ^^^ In addition , the distribution patterns of NR 1 , NR2A , and NR2B contaning NMDA receptors in rat stomach were determined immunohistochemically by using specific antibodies against NR 1 , NR2A , and NR2B . ^^^ The fact that NMDA receptor subunits NR 1 , NR2A , and NR2B were found to be localized in the rat stomach as visualized immunohistochemically with specific antibodies against NR 1 , NR2A , and NR2B is consistent with this hypothesis . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR2A and NR2B protein concentrations were significantly decreased by about 30 % in diabetic rats . ^^^ Dietary LC PUFAs partially restored NR2A and NR2B in diabetic rats whereas the most significant increase was seen in nondiabetic rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The increased levels of extracellular glutamate , NMDA receptor zeta subunit ( NR 1 ) mRNA , NMDA receptor epsilon 1 subunit ( NR2A ) protein , phosphorylated Ca ( 2+ ) / calmodulin kinase 2 ( p CaMKII ) protein , c fos mRNA , c Fos protein , are observed in the specific brain areas of mice and / or rats showing signs of naloxone precipitated withdrawal . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Switching of the NMDA receptor 2A ( NR2A ) and NR2B subunits at NMDA receptors is thought to underlie the functional changes that occur in NMDA receptor properties during the developmental epoch when neural plasticity is most pronounced . ^^^ The cellular expression of NR2A and NR2B and the NR 2 synaptic binding protein postsynaptic density 95 ( PSD 95 ) was examined in the mouse somatosensory cortex and thalamus from postnatal day 2 ( P 2 ) to P 15 using reverse transcription PCR , in situ hybridization histochemistry , and immunocytochemistry . ^^^ The localization of NR2A and NR2B subunits and PSD 95 was then studied at synapses in layer 4 of somatosensory cortex and in the ventral posterior nucleus of the thalamus using high resolution immunoelectron microscopy . ^^^ At both cortical and thalamic synapses , a quantitative switch in the dominant synaptic subunit from NR2B to NR2A was accompanied by a similar change in the cellular expression of NR2A but not of NR2B . ^^^ Synaptic PSD 95 developed independently , although both NR2A and NR2B colocalized with PSD 95 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We demonstrate here that the NMDA receptor subunits NR2A and NR2B are not routinely expressed in cultured spinal neurons and that transfection with NR2A or NR2B reconstitutes the synaptic targeting of NMDA receptors and confers on exogenous application of the immediate early gene product Narp the ability to cluster both AMPA and NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The k ( + , app ) for NR1 / 2D receptors was similar to but moderately slower than the k ( + , app ) obtained from cortical NMDA receptors composed of NR 1 , NR2A and NR2B subunits at all voltages tested . ( 3 ) Mg ( 2+ ) ( o ) blocking events induced an additional component in the closed duration distribution ; this component was used to estimate the apparent unblocking rate of Mg ( 2+ ) ( o ) ( k ( , app ) ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Long term NR2B expression in the cerebellum alters granule cell development and leads to NR2A down regulation and motor deficits . ^^^ To analyze the importance of NR2B replacement by NR2C and NR2A subunits until the end of the first month of age , we generated mice with lasting NR2B expression but deficiency for NR2C ( NR2C 2B mice ) . ^^^ Increased NR2B subunit levels led also to a gradual down regulation of cerebellar NR2A levels , preceding the development of motor impairment in adult animals . ^^^ Therefore , cerebellar NR2A is important for proper motor coordination and can not be replaced by long term expression of NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In situ hydridization experiments demonstrated overexpression of the mRNAs for the N methyl D aspartate ( NMDA ) receptor NR2A and NR2B subunits in mice devoid of normal cellular prion protein . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Since the normal function of NMDA receptor is critical for synaptogenesis , the long term effects of toluene exposure during synaptogenesis on the neurobehavioral function and the expression of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) were examined . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analysis revealed that prolonged toluene exposure increased levels of NR2A and NR2B NMDA receptor subunits with no change in NR 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Selective subunit antagonists suggest an inhibitory relationship between NR2B and NR2A subunit containing N methyl D : aspartate receptors in hippocampal slices . ^^^ Here , we have examined the effects of selective antagonists of NR2A and NR2B subunits ( NVP AAM 07 and Ro 25 6981 respectively ) on the effects of NMDA in the CA 1 field of rat hippocampal slices . ^^^ The results suggest that , at both presynaptic and postsynaptic sites in the rat hippocampus , NR2B subunit containing receptors limit NMDA receptor function by inhibitory restraint over NR2A subunit containing receptors , via calcineurin activation , and that LTP induction critically involves primarily receptors containing the NR2A subunit . ^^^ Endogenous factors or drugs that modify this NR2B / NR2A interaction could have a major influence on synaptic transmission and plasticity in the brain . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Pretreatment with NT 3 enhanced the vulnerability of ES neurons to NMDA induced death ( 100 microM NMDA for 10 min ) and enhanced the NMDA induced increase in neuronal [ Ca2+ ] 1 , but did not alter expression of NMDA receptor subunits NR2A or NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We examined the ability of postsynaptic density 95 ( PSD 95 ) protein to alter the calpain mediated cleavage of NR2A and NR2B . ^^^ Coexpression of PSD 95 with NMDA receptors in human embryonic kidney 293 cells blocked cleavage of NR2A and NR2B by NMDA receptor activated calpain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We studied the distribution pattern of different subunits of the NMDA receptor within the rat retina including quantifying the pattern of labelling for all the NRI splice variants , the NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Synaptic distribution of the NR 1 , NR2A and NR2B subunits of the N methyl d aspartate receptor in the rat lumbar spinal cord revealed with an antigen unmasking technique . ^^^ We have used an antigen unmasking method involving pepsin treatment to reveal NR 1 , NR2A and NR2B subunits and have compared their distribution with that of the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionic acid receptor GluR 2 subunit , which is thought to be present at most glutamatergic synapses throughout the spinal cord . ^^^ Although NR 1 puncta were present throughout the grey matter , NR2A was concentrated in laminae 3 4 and NR2B in laminae 1 2 . ^^^ In laminae 1 2 , most NR2B puncta were also NR 1 immunoreactive and a similar arrangement was found for NR2A / NR1 in laminae 3 4 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Cellular and synaptic distribution of NR2A and NR2B in macaque monkey and rat hippocampus as visualized with subunit specific monoclonal antibodies . ^^^ Toward this end , mouse monoclonal antibodies were raised against fusion protein antigens corresponding to the putative amino acid sequences of human NMDA receptor subunits NR2A and NR2B . ^^^ At the light microscopic level , both NR2A and NR2B exhibit a distribution in monkey and rat hippocampus very similar to NMDA receptor subunit NR 1 , and both are highly colocalized with NR 1 . ^^^ Electron microscopic immunogold studies demonstrated that both NR2A and NR2B are often present in asymmetric synapses in CA 1 , commonly colocalized with NR 1 , and often colocalized with each other in the same asymmetric synapses . ^^^ The confocal and ultrastructural data suggest that whereas NR 1 , NR2A , and NR2B are essentially uniformly colocalized in hippocampal projection neurons , there is extensive heterogeneity at the synaptic level that would lead to multiple functional classes of NMDA receptor mediated synapses , and extensive capacity for plasticity at the synapse . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Consistent with the depressant effects of L Phe , expression of NMDA receptor NR2A and ( RS ) amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid ( AMPA ) receptor Glu 1 and Glu2 / 3 subunits was significantly increased , whereas expression of the NR2B subunit was decreased . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In NMDA receptors , the NTDs of NR2A and NR2B form modulatory domains binding allosteric inhibitors . ^^^ Whereas the NTD of NR2A binds zinc with high ( nanomolar ) affinity , the NTD of NR2B binds the synthetic neuroprotectant ifenprodil and its derivatives . ^^^ Given that zinc is accumulated and released at many glutamatergic synapses in the CNS , these findings suggest that zinc is the endogenous ligand of the NTD of both NR2A and NR2B , the two major NR 2 subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Whole cell NMDA current density decreased with development in both strains though currents from NR2A / neurones demonstrated greater sensitivity to CP 101 606 , an NR2B subunit specific blocker . ^^^ Qualitatively the overall NR2B subunit staining pattern was similar in + / + and NR2A / neurones throughout development , suggesting that the NR2B subunit distribution is not mediated by the NR2A subunit . ^^^ In addition , staining with markers for excitatory synapses showed that expression of NR2A subunit ( but not NR2B ) increases at both synaptic and extrasynaptic sites in + / + neurones during development . ^^^ In parallel , NMDA mEPSCs were faster in + / + compared with NR2A / neurones at all time points studied , suggesting that the NR2A subunit begins to replace NR2B rich NMDA receptors even at early stages of development . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Myosin RLC co localized with NR 1 in the dendritic spines of isolated hippocampal neurons , and was co immunoprecipitated from brain extracts in a complex with NR 1 , NR2A , NR2B , PSD 95 , Adaptor protein 2 and myosin 2 heavy chain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The expression of NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) at 5 14 days in vitro ( DIV ) were also determined . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The present study characterized the long term effects of toluene exposure during synaptogenesis on the expression of NMDA receptor subunits ( NR 1 , NR2A and NR2B ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Structural features of the glutamate binding site in recombinant NR1 / NR2A N methyl D aspartate receptors determined by site directed mutagenesis and molecular modeling . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B was also increased , suggesting that ShcC mutant mice have enhanced NMDA receptor function in the hippocampus . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
All antagonists displayed the potency order ( high to low affinity ) of NR2A > NR2B > NR2C > NR2D , however the longer chain antagonists ( having 7 instead of 5 bond lengths between acidic groups ) displayed much greater subunit selectivity than their short chain homologues . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RESULTS : Our data confirm the mRNA expression of the NR 1 , NR2A , NR2B and NR2D subunits of NMDA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of the NR2A and NR2B subunits of the NMDAR increased 3 4 fold over control values during SE , continued to increase during the first hour following SE and then declined to control levels by 24 h . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
PKC site mutations reveal differential modulation by insulin of NMDA receptors containing NR2A or NR2B subunits . ^^^ The NR2A C terminus contains two residues homologous to the NR2B PKC targets . ^^^ Insulin modulation of NR2A containing receptors is mediated solely by PKCs , whereas insulin modulation of NR2B containing receptors is mediated by PKCs and tyrosine kinases ( PTKs ) . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Quantitative immunoblotting was used to determine the subcellular abundance of NR 1 , NR2A and NR2B subunits in striata from unlesioned , MPTP lesioned ( parkinsonian ) and MPTP lesioned , levodopa treated ( dyskinetic ) macaques . ^^^ In parkinsonian macaques , NR 1 and NR2B subunits in synaptosomal membranes were decreased to 66 + / 11 % and 51 . 2 + / 5 % of unlesioned levels respectively , while the abundance of NR2A was unaltered . ^^^ Levodopa treatment eliciting dyskinesia normalized NR 1 and NR2B and increased NR2A subunits to 150 + / 12 % of unlesioned levels . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Although the expression levels of NMDA receptor subunits and interaction of NMDA receptor and postsynaptic density 95 did not alter quantitatively , there was a specific alteration in NR2A , but not NR2B , subunit tyrosine phosphorylation after NMDA stimulation in the FRFS group . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Following ischemia , the phosphorylation of NR 1 by protein kinase C and tyrosine phosphorylation of NR2A and NR2B was increased in both lipid rafts and PSDs , with a greater increase in tyrosine phosphorylation occurring in the raft fraction . ^^^ Following ischemia , NR 1 , NR2A and NR2B levels were elevated in PSDs and reduced in lipid rafts . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Typical antagonists such as ( R ) AP 5 have a subunit selectivity of NR2A > NR2B > NR2C > NR2D . ^^^ The competitive NMDA receptor antagonist ( 2R , 3S ) ( 1 biphenylyl 4 carbonyl ) piperazine 2 , 3 dicarboxylic acid ( PBPD , 16b ) displays an unusual selectivity with improved relative affinity for NR2C and NR2D vs NR2A and NR2B . ^^^ A phenanthrenyl 2 carbonyl analogue , 16e , had > 60 fold higher affinity for NR2C and NR2D and showed 3 5 fold selectivity for NR2C / NR2D vs NR2A / NR2B . ^^^ The phenanthrenyl 3 carbonyl analogue ( 16f ) was less potent but more selective , having 5 and 7 fold selectivity for NR2D vs NR2A and NR2B , respectively . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Functional roles of NMDA receptor NR2A and NR2B subunits in the acute intoxicating effects of ethanol in mice . ^^^ The present study examined the roles of NR2A and NR2B subunit containing NMDA receptors in the mediation of the sedative / hypnotic effects of ethanol in mice . ^^^ The ability of the competitive NMDA antagonist , CGP 37849 ( 0 , 1 , or 3 mg / kg ) , and the NR2B selective antagonist , Ro 25 6981 ( 0 , 3 , or 10 mg / kg ) , to alter ( 3 g / kg ) ethanol induced sleep time was measured in C57BL / 6J mice and NR2A knockout ( KO ) mice . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To determine whether neuropsychiatric manifestations in patients with systemic lupus erythematosus ( SLE ) are influenced by antibodies against the human N methyl D aspartate ( NMDA ) receptor types NR2a or NR2b . ^^^ A decapeptide was synthesized containing a sequence motif present in the extracellular ligand binding domain of NMDA receptors NR2a and NR2b , bound by the monoclonal murine anti DNA antibody R4A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 , NR2A or NR2B antisense ODNs were incubated alone or with PCP for 48h . ^^^ Co incubation of PCP with either NR 1 or NR2A antisense significantly reduced PCP induced apoptosis , while neither NR2B antisense ODN nor NR 1 sense ODN used as a control had an effect . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
To test this hypothesis further , we examined the effects of 10 daily s . c . injections of IGF 1 on NMDA receptor subunits ( NR 1 , NR2A , and NR2B ) , GH receptor ( GHR ) , GH binding protein ( GHBP ) and type 1 IGF receptor ( IGF 1R ) gene transcripts in the hippocampus . ^^^ The NR2B subunit mRNA increased in young ( 11 weeks ) but not in older ( 14 16 months ) rats and the expression of the NR2A mRNA was decreased in both groups . ^^^ The ratio of NR2B to NR2A is suggested to mirror the potential for synaptic plasticity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Using coimmunoprecipitation , Western blotting , and mass spectrometry , we show that inhibition of tyrosine phosphatases triggers disassembly of NR 1 , NR2A , and NR2B in cortical NMDA receptor complexes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
MK 801 not only decreased the expression of the NR 1 subunit of NMDA receptors but also increased NR2A expression , without affecting NR2B expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Two different models of brain ischemia were used to examine the evoked changes in the tyrosine phosphorylation of NMDA receptor subunits 2A and 2B ( NR2A and NR2B ) , as well as their interactions with non receptor tyrosine kinases ( NRTKs : FAK , PYK 2 Src ) , and PSD 95 protein . ^^^ In contrast , ischemia of longer duration ( up to 30 min ) caused an immediate decrease in the protein levels as well as tyrosine phosphorylation of both NR2A and NR2B subunits which was accompanied by the marked attenuation of the association with their investigated molecular partners PSD 95 and NRTKs . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Expression of NR2A , NR2B , and NR2D was observed in all three cell types while NR3A was much more abundant in pre Botzinger complex interneurons , which belong to the rhythm generator of respiratory activity . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Both GRGDSP and fibronectin caused rapid Src kinase dependent increases in tyrosine phosphorylation of NMDA receptor subunits NR2A and NR2B in synaptoneurosomes and acute hippocampal slices . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast , the extent of desensitization of NMDARs containing NR2A in place of NR2B is significantly decreased by co expression of CaMKIIalpha . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The results showed that NR 1 , NR2A , and NR2B subunits could form homodimers when they were expressed alone in HEK 293 cells . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The hybridization signals for NR2A mRNA and NR2B mRNA were significantly elevated in the deep layer of the dorsal horn of diabetic rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Western blot analysis of NMDA receptor subunit expression showed that , at 7 days of withdrawal , the level of protein for NR2A and NR2B subunits was elevated in the CA 1 region of hippocampal slices from CIE treated animals compared with slices from age matched controls . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast to NRI and NR2C , very weak immunoreactivity of NR2A and NR2B subunits was observed in the LC . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Differential roles of NR2A and NR2B containing NMDA receptors in Ras ERK signaling and AMPA receptor trafficking . ^^^ In mature cultured neurons , NR2A NMDARs promote , whereas NR2B NMDARs inhibit , the surface expression of GluR 1 , primarily by regulating its surface insertion . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At CBF 30 > 9 min , we found downregulation of mRNA for NR2A , NR2B , and NR3B at 3 5 h after SAH , whereas the levels of NR 1 mRNA were unaffected . ^^^ The downregulation of NR2A and NR2B mRNA may result in a reduced NMDA receptor function . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Basolateral and central amygdala tissues were assayed for protein expression of the alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid ( AMPA ) receptor subunits ( GluR 1 and GluR 2 ) and the NMDA receptor subunits ( NR 1 , NR2A and NR2B ) . ^^^ In the basolateral amygdala , GluR 1 but not GluR 2 levels were increased on days 1 and 30 , NR2A levels were increased on day 1 , and NR2B levels were decreased on day 30 of withdrawal from cocaine . ^^^ In the central amygdala , GluR 2 but not GluR 1 levels were increased on days 1 and 30 , NR 1 levels were increased on day 30 and NR2A or NR2B levels were not altered after withdrawal from cocaine . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we report that covalent modification of NR 1 A652C or the analogous mutation in NR2A , 2B , 2C , or 2D by methanethiosulfonate ethylammonium ( MT SEA ) occurs only in the presence of glutamate and glycine , and that modification potentiates recombinant NMDA receptor currents . ^^^ The degree of potentiation depends on the identity of the NR 2 subunit ( NR2A < NR2B < NR2C , D ) inversely correlating with previous measurements of channel open probability . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NOS activities in the hippocampus , the cerebral cortex , the cerebellum and the brain stem were determined with fluorescence measurement and levels of mRNA expression of the NMDA receptor 2A ( NR2A ) subunit and NMDA receptor 2B ( NR2B ) subunit in the rat hippocampus were measured with Retro translation ( RT PCR ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
At PN 2 , expression of whole brain NMDA receptor subunits NR 1 , NR2A , and NR2B was lower in pups from dams fed ZD and MZD compared to controls , as analyzed using relative RT PCR and immunoblotting . ^^^ At PN 11 , whole brain and hippocampi NR 1 , NR2A , NR2B and PSA NCAM ( polysialic acid neural cell adhesion molecule ) expression and the number of PSA NCAM immunoreactive cells were lower in pups from dams fed ZD compared to controls . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The ethanol sensitive NMDA receptor subunits NR 1 , NR2A and NR2B were quantified by Western immunoblot analysis . ^^^ Exposure to ethanol ( 50 mM ) caused an increase in the levels of NR 1 ( 137 + / 11 % of untreated control , P = 0 . 009 ) , NR2A ( 128 + / 14 % , P = 0 . 022 ) and NR2B ( 136 + / 19 % , P = 0 . 012 ) . ^^^ Coincubation with memantine ( 10 microM ) completely blocked the ethanol induced up regulation of NR 1 ( 102 + / 4 % ) , NR2A ( 95 + / 7 % ) and NR2B ( 105 + / 13 % ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we show that exposure of hippocampal neurons to ethanol increases the internalization of the NR2A but not NR2B subunit of the NMDAR via the endocytic pathway . ^^^ Importantly , ethanol treatment alters functional subunit composition from NR2A / NR2B to mainly NR2B containing NMDARs . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptor ( NMDAR ) 2A ( NR2A ) and NR2B type NMDARs coexist in synapses of CA 1 pyramidal cells . ^^^ Recent studies using pharmacological blockade of NMDAR subtypes proposed that the NR2A type is responsible for inducing long term potentiation ( LTP ) , whereas the NR2B type induces long term depression ( LTD ) . ^^^ This contrasts with the finding in genetically modified mice that NR2B type NMDARs induce LTP when NR2A signaling is absent or impaired , although compensatory mechanisms might have contributed to this result . ^^^ Partial blockade of NMDA EPSCs by 40 % , either by preferentially antagonizing NR2A or NR2B type NMDARs or by the nonselective antagonist D AP 5 , did not impair LTP , demonstrating that hippocampal LTP induction can be generated by either NMDAR subtype . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This study administered chronic nicotine to determine the alterations of N methyl D aspartate receptor subunit 2A and 2B ( NR2A , NR2B ) and the alterations of alpha7nAChR receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Roles of NMDA receptor NR2A and NR2B subtypes for long term depression in the anterior cingulate cortex . ^^^ In the hippocampus , the NR2A subunit is believed to be involved in the induction of LTP , whereas the NR2B subunit contributes to the formation of LTD . ^^^ Furthermore , our studies provide direct physiological evidence that both NR2A and NR2B subunits are involved in the induction of LTD in the ACC . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunoblot analyses from behaviorally symptomatic Mecp 2 null mice reveal altered expression of N methyl d aspartate receptor subunits NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we show that in transgenic mice overexpressing the human AD gene APPswe ( Tg 2576 ) , safflower oil induced n 3 PFA deficiency caused a decrease in N methyl D aspartate ( NMDA ) receptor subunits , NR2A and NR2B , in the cortex and hippocampus with no loss of the presynaptic markers , synaptophysin and synaptosomal associated protein 25 ( SNAP 25 ) . n 3 PFA depletion also decreased the NR 1 subunit in the hippocampus and Ca2+ / calmodulin dependent protein kinase ( CaMKII ) in the cortex of Tg 2576 mice . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Thus , we report two NMDAR 1 genes ( NR1 . 1 and NR1 . 2 ) , and eight NMDAR 2 genes , designated NR2A . 1 and NR2A . 2 , NR2B . 1 and NR2B . 2 , NR2C . 1 and NR2C . 2 , and NR2D . 1 and NR2D . 2 . ^^^ NR 2 developmental gene expression varies : both paralogs of the NR2A are expressed at 48 hpf in the retina , only one paralog of the NR2B is expressed at low levels in the heart at 48 hpf . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We studied adsorption capacity of 5 latexes to synthetic peptide fragments of mu and delta opioid receptors and to GluR 1 and NR2A subunits of glutamate receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
There are at least eight splicing variant isoforms of the NR 1 subunit and four types of NR 2 subunits : NR2A , NR2B , NR2C and NR2D . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Secondly , we used immunoblotting and immunohistochemistry to investigate the effects of nerve injury on NMDA receptor subunit expression , revealing increased expression of NR2B , but not NR2A and reduced NR 1 in the superficial dorsal horn . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Consistent with this specific pattern of effects caused by L Phe in neuronal cultures , the expression of NMDA receptor NR2A and AMPA receptor Glu 1 and Glu2 / 3 subunits in brain of hyperphenylalaninemic PKU mice ( Pah ( enu 2 ) strain ) was significantly increased , whereas expression of the NMDA receptor NR2B subunit was decreased . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction ( RT PCR ) analysis of single neurons revealed that the N methyl d aspartate ( NMDA ) receptor NR2B subunit is expressed more frequently in enkephalinergic neurons , and the NR2A subunit more frequently in non enkephalinergic neurons . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast , the NR2A subunit localizes to the synapse in the absence of PDZ binding and is not altered by mutations in its motif corresponding to YEKL of NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In particular , it has been suggested that NR2A containing NMDA receptors are involved in LTP induction , whereas NR2B containing receptors are involved in LTD induction in the hippocampus . ^^^ Here we report that the activation of the NR2B and NR2A subunits of the NMDA receptor is critical for the induction of cingulate LTP , regardless of the induction protocol . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Allosteric interaction between zinc and glutamate binding domains on NR2A causes desensitization of NMDA receptors . ^^^ Previous work suggests that fast desensitization of NR1 / NR2A receptors is caused by ambient zinc , and that a positive allosteric interaction occurs between the extracellular zinc binding amino terminal domain and the glutamate binding domain of NR2A . ^^^ Here we demonstrate that this allosteric coupling reflects interactions within the NR2A subunit , and that the affinity of zinc for its binding site is regulated by glutamate binding and not by glycine binding nor by channel pore opening . ^^^ We subsequently use this model to evaluate the effects of extracellular zinc on NR1 / NR2A excitatory postsynaptic currents ( EPSCs ) by simulating the response to a brief synaptic like pulse of glutamate . ^^^ Modelling results show that zinc at a steady state concentration of at least 100 nm has a significant effect on the amplitude of NMDA EPSCs but that concurrent release of 10 microm zinc with synaptic glutamate release has little effect on the amplitude of a single NR1 / NR2A NMDA EPSC . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We investigated in the rat the effect of axotomy on the different subunits of excitatory glutamatergic AMPA ( GLuR 1 4 ) , NMDA ( NR 1 , NR2A D ) receptors , post synaptic density 95 , vesicular glutamate transporter 2 , beta catenin and cadherin . mRNA levels and / or protein production were analyzed 1 , 3 , 8 , 30 and 60 days after facial nerve axotomy by in situ hybridization and immunohistofluorescence . mRNAs coding for the GLuR 2 4 , NR 1 , NR2A , B , D subunits of glutamatergic receptors and for post synaptic density 95 , were less abundant after axotomy . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The same compounds were preliminarily assayed using Xenopus oocytes expressing cloned rat NMDA receptors containing the NR 1 subunit in combination with either NR2A , NR2B , NR2C , or NR2D subunit . ^^^ In this assay , all three derivatives showed high antagonist potency with preference for the NR2A and NR2B subtypes , with derivative ( ) 4 behaving as the most potent antagonist . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the developing hippocampus , the N methyl D aspartate ( NMDA ) receptor subunit NR2B triggers long term potentiation , fundamental to learning and memory ; this is supplemented by the less plastic NR2A subunit in the adult . ^^^ To understand the mechanism of learning deficits in FAS , we evaluated NR2B and NR2A expression in embryonic and adult mice . ^^^ Calibrator normalized relative real time polymerase chain reaction was performed for NR2B and NR2A with glyceraldehyde 3 phosphate dehydrogenase standardization . ^^^ In the adult brains there was a relative decrease in NR2B ( P = . 03 ) and an increase in NR2A ( P < . 01 ) . ^^^ CONCLUSION : Prenatal alcohol exposure during development induces NR2B expression deregulation in the embryos that persists until adulthood , when a relative increase in the less modifiable subunit NR2A occurs . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The objective of this study is to determine the effects of Pb2+ on N methyl d aspartate receptor ( NMDAR ) subunits NR1C1 , NR2A and NR2B in primary cultured neuronal cells . ^^^ Aliquots of NR 1 , NR2A and NR2B proteins from cell homogenate were immunoprecipitated with protein A agarose and detected by Western blotting . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Switching synaptic NR2B containing NMDA Rs that bind CaMKII with high affinity with those containing NR2A , a subunit with low affinity for CaMKII , dramatically reduces LTP . ^^^ The switch from NR2B to NR2A content in synaptic NMDA Rs normally observed in many brain regions may contribute to reduced plasticity by controlling the binding of active CaMKII . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here , we show that the stoichiometry of NR2A and NR2B tyrosine phosphorylation is significantly higher in synaptosomal membranes than intracellular microsomal / light membranes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Within the nigrostriatal pathway , NR 1 , NR2A , NR2B , GluR 1 , GluR6 / 7 and KA 2 were increased in the dorsal CPu , whereas no significant changes were observed in the SN . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Here we report crystal structures of the ligand binding core of NR2A with glutamate and that of the NR 1 NR2A heterodimer with glutamate and glycine . ^^^ The NR2A glutamate complex defines the determinants of glutamate and NMDA recognition , and the NR 1 NR2A heterodimer suggests a mechanism for ligand induced ion channel opening . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RT PCR analysis showed that human resting peripheral blood lymphocytes ( PBL ) and Jurkat T cells express genes encoding for both NR 1 and NR2B subunits : phytohemagglutinin ( PHA ) activated PBL also expresses both these genes and the NR2A and NR2D genes . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the adult cerebral cortex , the receptor properties are regulated by relative expression of NR2A and NR2B subunits . ^^^ Here , we examined the effect of learning induced cortical reorganization upon expression of NR2A and NR2B NMDA receptor subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In surviving neurons , multiple changes occurred in the proportion of individual NMDA receptor subunit 1 ( NR 1 ) splice variant mRNA expression , whereas the overall expression of NR 1 , NR2A and NR2B transcripts remained unaffected . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
While there were no alterations in the glutamate sensitivity , channel open probability or the single channel conductance of the receptor , a crosslinking assay demonstrated that the NR 1 and NR2A subunits of NMDA receptors were preferentially retained in the cytoplasm after SD , indicating that SD alters NMDAR surface expression . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We also found that the PDZ 2 domain of PSD 95 interacted with the NR2A and NR2B subunits of NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In this study , we examined if traumatic mechanical injury would produce regional activation of calpain and caspase 3 in the in vitro hippocampus and studied how the mechanically induced activation of NR2A and NR2B containing N methyl d aspartate receptors ( NMDARs ) affects the activation of these proteases following mechanical injury . ^^^ Together , these studies suggest that the injury induced activation of NR2A containing NMDARs functions as a pro survival signal , while the activation of NR2B containing NMDARs is a competing , anti survival , signal following mechanical injury to the hippocampus . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
This decrease involved NR 1 C2 , NR 1 C2 ' , NR2A , and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NR 1 , NR2A , NR2B , NR2C , NR2D and NR3A subunits showed clustered expression in cell processes , but NR3B was absent . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In contrast , the Ca2+ increase in myelin was abolished by broad spectrum NMDA receptor antagonists ( MK 801 , 7 chlorokynurenic acid , d AP 5 ) , but not by more selective blockers of NR2A and NR2B subunit containing receptors ( NVP AAM 077 and ifenprodil ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The NR2A pre M 1 region , the linker between the glutamate binding domain and the channel pore , plays a critical role in desensitization . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Perinatal exposure to PTU delays switching from NR2B to NR2A subunits of the NMDA receptor in the rat cerebellum . ^^^ PTU induced up regulation of NR2B mRNA and down regulation of NR2A and BDNF mRNAs in the cerebellum on PND 22 , but there were no changes in the other genes ( growth associated protein 43 , L 1 , neuronal cell adhesion molecule , synaptophysin , post synaptic density 95 ) . ^^^ Examination of the effects of PTU on maturation of NMDAR subunits ( NR2A / NR2B ) demonstrated changes in relative expression on PND 14 , but not on PND 4 , with recovery after maturation . ^^^ The profile of NMDAR subunits in vehicle dosed rats showed a shift from NR2B to NR2A during development . ^^^ These results suggest PTU can delay this switching from NR2B to NR2A subunits in the maturation of NMDA receptors . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Recent work has shown that a subset of lupus Abs can crossreact with the NR2A and NR2B subunits of the NMDA receptor . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Furthermore , brain regions enriched in NMDAR 2A ( NR2A ) and NMDAR 2B ( NR2B ) subunits , such as the hippocampus , are particularly vulnerable . ^^^ Using cultured rat hippocampal cells and HIV 1 infected human monocyte derived macrophages ( HIV / MDM ) , we examined the role of NR2A and NR2B in HIV / MDM induced hippocampal neuronal death . ^^^ We found the following : ( 1 ) hippocampal neuronal susceptibility to HIV / MDM excitotoxins varies according to the developmental expression patterns of NR2A and NR2B ; ( 2 ) NMDAR activation by HIV / MDM results in neuronal calpain activation , which results in neuronal death ; and ( 3 ) selective antagonists of homomeric NR2B / NR2B and heteromeric NR2A / NR2B containing NMDARs , as well as an inhibitor of calpain activity , afford neuroprotection against HIV / MDM . ^^^ These studies establish a clear link between macrophage HIV infection , neuronal NR2A and NR2B activation , and calpain mediated hippocampal neuronal death . ^^^ They further suggest a dominant role for NR2A and NR2B in determining neuronal susceptibility in HIV infected brain . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The most prominent increase was of two 180 kDa phosphoproteins , which were identified as the NR2A and NR2B subunits of NMDA R . ^^^ We also found that the increase in NR2A and NR2B proteins in heterozygous reeler was dependent on Fyn , because this change was absent in heterozygous reeler / homozygous Fyn deficient double mutant mice . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the first presentation , Dorit Ron presented data showing how activation of Fyn or Src tyrosine kinases differentially regulated the cell surface expression and activity of NR2A and NR2B containing NMDA receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In this study we examined the effects of chronic morphine administration on gene and protein expression of three major NMDA receptors subunits ( NR 1 , NR2A , and NR2B ) in NAcc and CeA . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the auditory cortex , the properties of NMDA receptors depend primarily on the ratio of NR2A and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We did not detect any differences in immunoreactivity in any of the non phosphorylated NR 1 , NR2A , NR2B , NR2C or the NR2D subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In both HEK 293 cells and Xenopus laevis oocytes expressing recombinant NMDA receptors , Ni ( 2+ ) ( < 100 microM ) caused a potentiation of NR2B containing channels but a voltage independent inhibition in those containing NR2A . ^^^ We took advantage of this different response to investigate the developmental switch between NR2B and NR2A subunits in neonatal rat cerebellar granule cells up to 16 days in vitro ( DIV ) and in rat embryo cortical neurons up to 35 DIV . ^^^ All these data indicate that Ni ( 2+ ) is a suitable marker for the identification of NR2A and NR2B native channel subunits and can be used to trace the development of NMDA receptor composition . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Furthermore , the association between PSD 95 and NMDAR subunits ( NR 1 , NR2A , and NR2B ) in the hippocampal CA 1 was also markedly altered by perinatal hypoxia . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
We evaluated whether the mechanism of the peptide protection included NR2B , NR2A , and GABAAalpha 5 . ^^^ Calibrator normalized relative real time polymerase chain reaction ( PCR ) was performed using primers for NR2B , NR2A , and GABAAalpha 5 with GAPDH standardization . ^^^ In the adult , the peptides prevented NR2B down regulation ( P = . 01 ) and NR2A up regulation ( P < . 001 ) . ^^^ CONCLUSION : VIP related peptides prevented alcohol induced changes in NR2B , NR2A , and GABAAalpha 5 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Dyskinetic animals have significantly higher levels of NR2A subunit in the postsynaptic compartment than all other experimental groups , whereas NR2B subunit shows a significant reduction in both dopamine denervated and dyskinetic rats . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA induced changes in slices from young rats were blocked completely by the NMDA receptor antagonist ( + ) 5 methyl 10 , 11 dihydro 5H dibenzo [ a , d ] cyclohepten 5 , 10 imine maleate ( MK 801 ) and by the antagonists of NR2B receptor ifenprodil and R ( R , S ) alpha ( 4 hydroxyphenyl ) beta methyl 4 ( phenylmethyl ) 1 piperidine propranol and were partly blocked by calpain inhibitor 3 but were not affected by the NR2A specific antagonist [ ( R ) [ ( S ) 1 ( 4 bromo phenyl ) ethylamino ] ( 2 , 3 dioxo 1 , 2 , 3 , 4 tetrahydroquinoxalin 5 yl ) methyl ] phosphonic acid . ^^^ NMDA induced changes in Nissl staining were also different in slices from young and adult rats and blocked by NR2B but not NR2A antagonists . ^^^ Our results are consistent with the idea that NMDA mediated toxicity is caused by activation of NR2B but not NR2A containing NMDA receptors leading to calpain activation and that developmental changes in NMDA toxicity reflect developmental changes in NMDA receptor subunit composition . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Ventral compared with dorsal hippocampus showed for NMDA receptors : 1 ) lower levels of mRNA and protein expression for NR2A and NR2B subunits in CA 1 with the ratio of NR2A / NR2B differing between the two poles and 2 ) lower levels of [ ( 3 ) H ] MK 801 binding in the ventral hippocampus , with the lowest value observed in CA 1 , apparently resulting from a decreased receptor density since the B ( max ) value was lower in ventral hippocampus . ^^^ Consistently , the lower value of the ratio NR2A / NR2B seen in the ventral part would imply that the ventral hippocampus NMDA receptor subtype is functionally different than the dorsal hippocampus subtype , as supported by our intracellular recordings . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Experiments in vitro indicate that selective antagonism of NMDA receptors containing NR2A subunits blocks perirhinal long term potentiation ( LTP ) , whereas antagonism of NMDA receptors containing NR2B subunits blocks long term depression ( LTD ) . ^^^ However , recognition memory after a 24 h delay was impaired only when both an NR2A and an NR2B antagonist were infused together , not when either was infused separately . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
SE was induced using the Li / pilocarine model and phosphorylation of the NMDA receptor subunits NR2A and NR2B determined . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NMDA receptors composed of NR1 / NR2C exhibit a low conductance and reduced sensitivity to Mg ( 2+ ) , compared with the more commonly studied NR2A and NR2B containing receptors . ^^^ This residue is located adjacent to the consensus PDZ ligand , a region that regulates protein protein interactions and receptor trafficking in NR2A and NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Density of NMDA receptors was evaluated using [ 3H ] MK 801 autoradiography in 52 brain areas and expression of NR2A and NR2B subunits was studied with in situ hybridization in the same brains . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
According to the quantitative changes of NMDA receptor subunits , including NR 1 , NR2A , and NR2B , induced by diverse types of reinforcers , we chose NR2B subunit as the target of research . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Hippocampal mRNA expression of the NMDA subunits NR 1 , NR2A and NR2B , insulin like growth factor type 1 receptor ( IGF 1R ) , and postsynaptic density protein 95 ( PSD 95 ) was then measured in the animals by Northern blot analysis . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Blockers of tyrosine phosphatases also induce NMDA subunit trafficking , but this effect is nonselective and alters both NR2A and NR2B containing receptors . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Immunohistochemistry demonstrated that the NT 2 neurons are mostly GABAergic ; they predominantly express the NMDA receptor subunits NR2B and NR2C , and lower levels of NR 1 and , particularly , of NR2A . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the present study we used RT PCR methods on single cells and tissue to compare the expression of NMDAR subunits , NR 1 , NR2A and NR2B , in the striatum of R6 / 2 transgenic mice with their wild type ( WT ) littermates at three different age groups corresponding to different symptomatic milestones ( 19 25 days showing no overt evidence of abnormal behavior , 38 45 days at the onset of the overt phenotype and 78 90 days displaying the full behavioral phenotype ) . ^^^ Semi quantitative RT PCR studies on striatal tissue in the oldest age group confirmed the significant decrease in NR2A and also showed a decrease in NR2B . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Mutation of Ser 670 to glycine ( S670G ) in NR2A reduced the potency of glutamate by 124 fold . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Furthermore , the expression of mRNA for NR2A and NR2B subunits of the NMDA glutamatergic receptor in hippocampus of aged Prnp ( 0 / 0 ) animals showed an increase compared to the wild type . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
In the present study , we performed western blot analysis to determine whether protein levels of NMDA receptor subunits ( NR 1 , NR2A , NR2B ) and associated PSD proteins ( NF L , PSD 95 , SAP 102 ) are altered in schizophrenia . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
RT PCR analysis showed no significant changes in NR 1 , NR2A , or NR2B gene expression ; however , as a positive control , hsp 70 was induced more than twofold in ipsilateral cortex and hippocampus on PID 1 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
The IC 50 values for NVP AAM 077 acting at NR1 / NR2A NMDA receptors were , as expected , dependent on the glutamate concentration used to evoke responses , being 31 + / 2 nM ( with glutamate at its EC 50 concentration ) and 214 + / 10 nM ( at 10 times the EC 50 concentration ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
For the prefrontal cortex , no difference in mRNA expression was observed for NMDA NR2A and NR2B or AMPA GluR 1 and GluR 2 . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
METHODS : The effects of chronic antipsychotic drug administration ( haloperidol and clozapine ) on protein levels of NR 1 , NR2A and NR2B proteins were evaluated in the nucleus accumbens ( NAc ) , putamen ( PUT ) , dorsolateral prefrontal cortex ( DLPFC ) , superior temporal gyrus ( STG ) , and entorhinal cortex ( EC ) of rhesus monkeys using Western blot analysis . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of the NMDAR NR2A and NR2B subunits was reduced upon PTPalpha ablation , indicating a positive effect of this phosphatase on NMDAR phosphorylation via intermediate molecules . ^^^ In a human embryonic kidney ( HEK ) 293 cell expression system , PTPalpha enhanced fyn mediated NR2A and NR2B tyrosine phosphorylation by several fold . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Double immunostaining for HDC and NMDA receptor subunits showed that histamine neurons of the rat tuberomammillary nucleus express NMDA receptor subunit 1 ( NR 1 ) with NMDA receptor subunit 2A ( NR2A ) and NMDA receptor 2B subunit ( NR2B ) . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
A selective increase in the NR2B subunit was present in all cortical dysplasia , whereas the expression level of NR2A and NR2B subunits was significantly downregulated in all patients with heterotopia . ^^^ In cerebral heterotopia , the NR2A and NR2B downregulation was accompanied by less evident reduction of the SAP 97 and PSD 95 proteins of the MAGUK family , thus suggesting that NMDA impairment was associated with altered molecular structure of the postsynaptic membrane . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Also , the expression level of NMDA receptors 1 ( NR 1 ) , NMDA receptors 2A ( NR2A ) , NMDA receptors 2B ( NR2B ) and AMPA ( GluR 1 subunit ) were determined by grayscale analysis in immunohistochemical stained slices . ^^^ CONCLUSION : The protective effects of ketamine midazolam anesthesia on ischemic brain injury may related to decreasing NR2A and NR2B expression . . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
Previous work showed that permeant ions have profound effects on Mg2+ ( o ) block of NMDA receptors composed of NR 1 , NR2A , and NR2B subunits . ^^^
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q12879 and Q13224	Pubmed	SVM Score :0.0
NA