Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Immunocytochemically , they expressed a number of neuronal antigens , such as 200 kDa neurofilament protein , neuron specific enolase , microtubule associated protein 2 , tau protein , tubulin , neural cell adhesion molecule , Thy 1 . 2 , saxitoxin binding sodium channel protein , ChAT , tyrosine hydroxylase , serotonin , and glutamate . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In immunocytochemical studies , both clones expressed 200 kD neurofilament protein , neuron specific enolase , microtubule associated protein 2 ( MAP 2 ) , tau protein , neuronal cell adhesion molecule ( N CAM ) , HNK 1 , Thy 1 . 2 , saxitoxin binding sodium channel protein , and glutamate . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Whether the diminished differentiation of granule cells must be attributed only to serum deprivation or also to other differences in the composition of the culture medium remains to be established . 3H GABA was avidly taken up also by a population of cells which were not recognized by antibodies raised against GFAP , glutamate decarboxylase , and microtubule associated protein 2 . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Cultures were exposed to glutamate for 10 min and evaluated for glutamate neurotoxicity ( GNT ) 18 24 hr later by tyrosine hydroxylase ( TH ) immunostaining , microtubule associated protein 2 ( MAP 2 ) immunostaining , and radiolabeled dopamine uptake assay . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
For microtubule associated protein 2 ( MAP 2 ) , neurogranin , and the glutamate receptor subtype B flip , no correlation was observed in any cell field between synaptic enhancement and hybridization to the mRNA . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In the experiments with 32P labeled cells , the phosphorylation of microtubule associated protein 2 ( MAP 2 ) and synapsin 1 was stimulated by the exposure to glutamate . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Microtubule associated protein 2 expression appeared 6 h after plating , while glutamate receptor subunit expression was present at 2 h . ^^^ This indicates that microtubule associated protein 2 does not regulate the initial distribution of glutamate receptor subunits into neurites . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Intracortical perfusion of glutamate in vivo induces alterations of tau and microtubule associated protein 2 immunoreactivity in the rat . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In the present studies exposure of fetal hippocampal neurons to glutamate ( 100 microM ) for 5 min resulted in increases in the neuron specific growth associated genes T alpha 1 alpha tubulin ( T alpha 1 ) , microtubule associated protein 2 ( MAP 2 ) and growth associated protein 43 ( GAP 43 ) . mRNA levels peaked at between 8 and 12 h following exposure as determined by competitive reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Dual immunocytochemistry ( ICC ) revealed that glutamate ( 1 microM ) increased nuclear pCREB ir in cells immunoreactive for microtubule associated protein 2 ( MAP 2 ir ) , but not other cells , indicating an effect predominantly on neurons . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Finally , glutamate receptor activation leads to a dephosphorylation of high molecular weight microtubule associated protein 2 at this site which can also be effectively prevented by okadaic acid . ^^^ These results are consistent with the participation of glycogen synthase kinase 3 , extracellular signal regulated protein kinases and protein phosphatases 1 and 2A , in the regulation of microtubule associated protein 2 phosphorylation within living neurons , which may be modulated by extracellular signals like the neurotransmitter glutamate . . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Intracerebroventricular ( i . c . v . ) administration of the Group 1 metabotropic glutamate receptor ( mGluR ) agonist , quisqualate , produced seizures , akinesia , destruction of hippocampal pyramidal cell dendritic microtubule associated protein 2 , and major loss of hippocampal CA sector neurons . 4 . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
The aim of the present work was to analyze the effects of chronic exposure to ammonia on phosphorylation of microtubule associated protein 2 ( MAP 2 ) in intact neurons in culture and to assess whether modulation of MAP 2 phosphorylation by glutamate receptor associated transduction pathways is altered in neurons chronically exposed to ammonia . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Neither a 24 h pretreatment nor posttreatment with gamma vinyl GABA ( 100 microM ) had any neuroprotective effects , as determined by counting microtubule associated protein 2 positive cells and lactate dehydrogenase assay 24 h after the glutamate treatment . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Using triple label immnocytochemistry for activated caspase 3 , fragmentation of DNA and the microtubule associated protein 2 , we characterized treatment paradigms as models of the apoptotic ( staurosporine , camptothecin ) , the oncotic ( high dose menadione , glutamate ) , and the mixed apoptotic and oncotic ( low dose menadione ) pathways of neuronal death . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Axonal and dendritic compartments acquire their distinct composition at stage 4 ( 4 5days in culture ) , when proteins such as the microtubule associated protein 2 ( MAP 2 ) and the glutamate receptor subunit GluR 1 localize to the dendrites and disappear from the axon [ 4 , 5 ] . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
The number of differentiated retinal ganglion cells as determined by microtubule associated protein 2 labeling was significantly increased following exposure to low but not higher doses of glutamate . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In this report , we investigate the developmental time course of the dendritic bundles , visualized by immunohistochemistry for microtubule associated protein 2 ( MAP 2 ) and glutamate receptor subunits 2 / 3 ( GluR2 / 3 ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Cultures treated for up to 5 weeks with epidermal growth factor , fibroblast growth factor 2 , retinoic acid , and nerve growth factor displayed neuron like cellular processes and expressed neuronal markers , including the neuronal nuclear antigen NeuN , microtubule associated protein 2 , tau , synaptophysin , alpha ( 1A ) and alpha ( 1B ) calcium channel subunits , NR2A glutamate receptor subunits , and gamma aminobutyric acid . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Chronic exposure to ammonia alters the modulation of phosphorylation of microtubule associated protein 2 by metabotropic glutamate receptors 1 and 5 in cerebellar neurons in culture . ^^^ Hyperammonemia impairs signal transduction associated to glutamate receptors and phosphorylation of some neuronal proteins including microtubule associated protein 2 ( MAP 2 ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
To test whether the induction of multiple dopamine signaling molecules in the lateral ganglionic eminence was due to a general enhancement of neuronal differentiation by retinoic acid , we assayed the effects of retinoic acid on other differentiation markers , including glutamate decarboxylase 65 , NR 1 subunit of glutamate NMDA receptor and microtubule associated protein 2 . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Using microtubule associated protein 2 ( MAP 2 ) as a neuron specific marker , we assessed glutamate receptor mediated neurotoxicity in neuron enriched cultures and in mixed neuronal / glial cultures established from mouse forebrain and compared these results to neuronal death measured by lactate dehydrogenase ( LDH ) release . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Single label immunohistochemistry and double label immunofluorescence were used to study the expression of TRPC 1 among substantia nigra dopamine neurons and cellular processes using antibodies against tyrosine hydroxylase ( TH ) , substance P ( SP ) , enkephalin , synaptophysin , vesicular glutamate transporter 2 ( Vglut 2 ) , microtubule associated protein 2 and metabotropic glutamate receptor 1 ( mGluR 1 ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Measurement of the neuronal cytoskeletal microtubule associated protein , MAP 2 , was used to assess survival of neurons after exposure of neuronal cultures to glutamate . ^^^ Exposure of 7 day old neuronal cultures to 1 mM glutamate for 16 hours led to a three fold increase in released lactate dehydrogenase ( LDH ) and a 40 % decrease in cellular MAP 2 content . ^^^ A 16 hour exposure to 1 mM glutamate did not induce LDH release or changes in MAP 2 levels in sabeluzole treated cultures . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Both glutamate and chloride inhibit actin MAPs interaction , but this is compensated for in glutamate where the microtubules are longer than in chloride . 2 ) The low shear viscosity of mixtures of isolated MAPs and actin filaments is enhanced by acidic pH and inhibited by high ionic strength . 3 ) MAPs can be fractionated to yield four different fractions with actin cross linking activity : a subset of high molecular weight MAPs , purified `` MAP 2 ' ' and two different fractions of tau polypeptides . 4 ) We have reconstituted a gel from actin , purified tubulin , and whole MAPs , but have not yet been successful with actin , purified tubulin , and any single purified MAP . . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
The selective modulation of MAP 2 mRNA in dendrites reveals a mechanism allowing a sustained stimulation of dendritic outgrowth to be confined to those regions of a neuron ' s dendritic arbour local to glutamate receptor stimulation . . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Between E 15 and E 19 the following populations were distinguished in the plexiform layers : the subventricular zone displayed small neurons that reacted with MAP 2 and GABA antibodies ; the subplate ( prospective stratum oriens ) was poorly populated by MAP 2 and GABA positive cells ; the inner marginal zone ( future stratum radiatum ) was heavily populated by multipolar GABAergic cells ; the outer marginal zone ( stratum lacunosum moleculare ) displayed horizontal neurons that showed glutamate and calretinin immunoreactivities , their morphology being reminiscent of neocortical Cajal Retzius cells . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
M 213 2O is a multipolar , polygonal cell line which expresses SV 40 large T antigen and glutamate decarboxylase ( GAD ) at the permissive temperature ( 33 degrees C ) and GAD and MAP 2 immunoreactivity at the nonpermissive temperature ( 39 . 3 degrees C ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
With time in culture , these neurons establish a complex dendritic and axonal network , as indicated by staining with antibodies against microtubulin associated protein ( MAP 2 ) and neurofilaments ( SMI 32 and SMI 33 ) as well as GABA and glutamate decarboxylase mRNA immunoreactivity . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Postsynaptic mechanisms for bidirectional control of MAP 2 phosphorylation by glutamate receptors . ^^^ Here , the postsynaptic , neuron specific microtubule associated protein MAP 2 is identified as a target of bidirectional calcium dependent signaling pathways activated by glutamate . ^^^ Glutamate produced a biphasic change in MAP 2 : a rapid , transient increase in phosphorylation mediated by metabotropic receptors and attenuated by inhibitors of calcium / calmodulin dependent protein kinases and protein kinase C , followed by a persistent dephosphorylation of MAP 2 mediated by NMDA receptors and activation of the calcium / calmodulin dependent protein phosphatase 2B ( calcineurin ) . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In intact explants , maintained for up to 14 days in vitro , the preservation of neurones , neuronal subtypes and glia was confirmed with immunofluorescence staining for neurone specific nucleoprotein ( NeuN ) , microtubule associated protein ( MAP 2 ) , a glutamate receptor subunit ( GluR 1 ) , GABA and glial fibrillary acidic protein ( GFAP ) respectively . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
These data indicate that the mechanisms coupling glutamate release to MAP 2 dephosphorylation are relatively lacking in the neonatal hippocampus . ^^^ The neonatal propensity toward glutamate stimulated MAP 2 phosphorylation may serve to reduce cytoskeletal stability and permit dendritic arborization early in postnatal development . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Moreover , exposure to APV ( an antagonist of NMDA glutamate receptors ) counteracted the effect of NMDA and induced an increase in the phosphorylation state of these sequences in MAP 2 . ^^^ Since phosphorylation regulates the interaction of MAPs with cytoskeleton , the results suggest that the modulation of the phosphorylated state of MAP 2 by NMDA glutamate receptors may be implicated in dendritic plasticity . . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Finally , treatment of animals with riluzole , an inhibitor of glutamate release , before surgery reduced significantly the loss of MAP 2 levels observed at 24 h after injury . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Neuronal damage and MAP 2 changes induced by the glutamate transport inhibitor dihydrokainate and by kainate in rat hippocampus in vivo . ^^^ In the present work we studied the neurotoxic effects of the intrahippocampal injections of the glutamate transport inhibitor dihydrokainate as compared to those of kainate , as well as the concomitant changes in the microtubule associated protein MAP 2 . ^^^ These results suggest that early MAP 2 disruption may be a marker of the excitotoxicity due to activation of different glutamate receptors located in discrete hippocampal regions . . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Nicotine prevents glutamate induced proteolysis of the microtubule associated protein MAP 2 and glutamate neurotoxicity in primary cultures of cerebellar neurons . ^^^ Nicotine prevents glutamate induced proteolysis of the microtubule associated protein MAP 2 and disaggregation of the neuronal microtubular network . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Similarly , adult neurons can be cultured that fire action potentials and display immunoreactivity for neurofilament , MAP 2 , tau , and glutamate . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Neuron like cells were immunoreactive for neuron specific enolase , neurofilament 200 , glutamate , MAP 2 , and tau after 2 weeks in culture . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In hippocampus , MAP 2 has been reported to be dephosphorylated by activation of the NMDA type glutamate receptor , a key player in synaptic plasticity . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Similarly , glutamate ( 100 microM ) increased phospho CREB immunoreactivity selectively in MAP 2 immunopositive cells . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
The possible neuropathological consequences of these two early MAP 2 changes are discussed in relation to the both excessive stimulation of glutamate receptors and subsequent dendritic spine alterations occurring in hippocampal neurons soon after soman intoxication . . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Interestingly , the properties of basal neurotransmission are normal in the Mecp 2 null mice , consistent with our observations that the levels of expression of synaptic and cytoskeletal proteins , including glutamate receptor subunits GluR 1 and GluR 2 , PSD 95 , synaptophysin 1 , synaptobrevin 2 , synaptotagmin 1 , MAP 2 , betaIII tubulin and NF 200 , are not significantly altered . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Exposure of cells , after 5 days in vitro , to glutamate 0 . 5 mM for 10 min caused morphological alterations , including disaggregation of beta tubulin and MAP 2 cytoskeletal protein assembly , loss of neurites and cell shrinkage . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
The aim is to elucidate 1 ) to what extent the functional disturbance of the cerebral cortex , as measured with electrocortical brain activity ( ECBA ) , is related to cerebral cortical tissue damage , as estimated by MAP 2 ; and 2 ) whether there is a relationship between the glutamate , nitric oxide ( NO ) , cGMP pathway and the development of cerebral cortical tissue damage after hemorrhagic hypotension . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
Neither PKA nor MAP2B were detected in immunoprecipitates of alpha amino 3 hydroxy 5 methylisoxazole 4 propionic acid type glutamate receptors or class B N type Ca ( 2+ ) channels . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
In this study , we examined the ability of G protein coupled metabotropic glutamate receptors ( mGluRs ) to inhibit the formation of processes induced by the expression of the microtubule associated protein MAP2c . ^^^
Interacting proteins: P11137 and Q13224 Pubmed SVM Score :0.0
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