| Interacting proteins: O00418 and Q13131 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00418 and Q13131 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00418 and Q13131 |
Pubmed |
SVM Score :0.0 |
| Therefore , the activation of eEF 2 kinase by AMPK , resulting in the phosphorylation and inactivation of eEF 2 , provides a novel mechanism for the inhibition of protein synthesis . . ^^^ |
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| Interacting proteins: O00418 and Q13131 |
Pubmed |
SVM Score :0.0 |
| Human recombinant eEF 2 kinase ( eEF2K ) was phosphorylated by AMPK in a time and AMP dependent fashion , and phosphorylation led to eEF2K activation , similar to that observed in extracts from ischemic hearts . ^^^ |
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| Interacting proteins: O00418 and Q13131 |
Pubmed |
SVM Score :0.0 |
| Here we show that AMPK directly phosphorylates eEF 2 kinase , and we identify the major site of phosphorylation as Ser 398 in a regulatory domain of eEF 2 kinase . ^^^ AMPK also phosphorylates two other sites ( Ser 78 and Ser 366 ) in eEF 2 kinase in vitro . ^^^ We develop appropriate phosphospecific antisera and show that phosphorylation of Ser 398 in eEF 2 kinase is enhanced in intact cells under a range of conditions that activate AMPK and increase the phosphorylation of eEF 2 . ^^^ Although cardiomyocytes appear to contain a distinct isoform of eEF 2 kinase , it also contains a site corresponding to Ser 398 that is phosphorylated by AMPK in vitro . ^^^ Stimuli that activate AMPK and increase eEF 2 phosphorylation within cells increase the activity of eEF 2 kinase . ^^^ |
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| Interacting proteins: O00418 and Q13131 |
Pubmed |
SVM Score :0.0 |
| We have recently shown that eEF 2 kinase is also controlled by phosphorylation by AMP activated protein kinase ( AMPK ) , a key regulator of cellular energy homeostasis . ^^^ Activation of AMPK also resulted in decreased p70S6 kinase phosphorylation and increased phosphorylation of eEF 2 , suggesting that inhibition of protein synthesis involves the eEF 2 kinase / eEF2 axis and / or the p70S6 kinase pathway . ^^^ |
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