| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Increased levels of TRAF 1 , TRAF 2 , and TANK / 1 TRAF were expressed in astrocytomas compared with levels in normal brain tissue ( p = 0 . 02 , 0 . 006 , and 0 . 01 , respectively ) . ^^^ The authors investigated the expression of NF kappaB , caspase 3 , TRAF 1 , TRAF 2 , and TRAF associated NF kappaB activator / TRAF interacting protein ( TANK / I TRAF ) , a regulator of TRAF activity , in human gliomas . ^^^ The NF kappaB activation was analyzed using the electrophoresis mobility shift assay ; TRAF 1 , TRAF 2 , TANK / I TRAF , and caspase 3 expression were studied using Western blot analysis . ^^^ A constitutive expression of TRAF 1 , TRAF 2 , and TANK / I TRAF in human gliomas was documented . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The C terminus possesses substantial homology to the tumor necrosis factor receptor associated factor ( TRAF ) domain that is found in two proteins ( TRAF 1 and TRAF 2 ) that associate with the cytoplasmic domain of the related 75 kDa tumor necrosis factor receptor . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The CAP 1 protein contains a C terminal domain that shares strong amino acid sequence homology with a unique domain found recently in two putative signal transducing proteins that bind to the TNF R 2 cytosolic tail , TRAF 1 and TRAF 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| A region of CRAF 1 was similar to the TNF alpha receptor associated factors TRAF 1 and TRAF 2 and so defined a shared TRAF C domain that was necessary and sufficient for CD 40 binding and homodimerization . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Overexpression of TRAF 2 , but not TRAF 1 or TRAF 3 , was sufficient to induce NF kappa B activation . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TNF receptor associated factor 1 ( TRAF 1 ) and TRAF 2 are the first two members of a novel protein family containing a novel C terminal homology region , the TRAF domain . ^^^ TNF receptor associated factor 1 ( TRAF 1 ) and TRAF 2 are the first two members of a novel protein family containing a novel C terminal homology region , the TRAF domain . ^^^ TRAF 1 and TRAF 2 can form homo and heterotypic dimers . ^^^ Our analysis indicates that TRAF 1 and TRAF 2 are associated with the cytoplasmic domain of TNF R 2 in a heterodimeric complex in which TRAF 2 contacts the receptor directly . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Two of these signal transducers , TRAF 1 and TRAF 2 , were isolated and characterized previously . ^^^ The c IAPs do not directly contact TNFR 2 , but rather associate with TRAF 1 and TRAF 2 through their N terminal BIR motif comprising domain . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Isolation and characterization of death domain ( TNF RI , Fas , TRADD , FADD / MORT 1 , RIP ) and TRAF domain containing proteins ( TRAF 1 , TRAF 2 , TRAF 3 ) have partially bridged a large molecular gap within one of several signaling pathways which originate at the plasma membrane and terminate in the nucleus . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| These results indicate that TRAF 1 and / or TRAF 2 play an important role in cell death in addition to their previously identified roles in cell proliferation . . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| As MIHB and MIHC were able to bind to the tumor necrosis factor receptor associated factors TRAF 1 and TRAF 2 in yeast two hybrid assays , these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE like proteases . . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The ability of TRAF 1 , TRAF 2 , and CRAF 1 to associate with CD 30 was confirmed using an in vitro coprecipitation assay , further demonstrating that the interaction was specific and direct . ^^^ CRAF 1 bound only a single site , which contained the sequence PEQET , whereas TRAF 1 and TRAF 2 were capable of binding to either the PEQET site or an additional downstream domain . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRAF 1 and TRAF 2 form an oligomeric complex that associates with the cytoplasmic domains of various members of the tumor necrosis factor ( TNF ) receptor superfamily . ^^^ Here we show that TRAF 1 and TRAF 2 interact with A 20 , a zinc finger protein , whose expression is induced by agents that activate NF kappaB . ^^^ Mutational analysis revealed that the N terminal half of A 20 interacts with the conserved C terminal TRAF domain of TRAF 1 and TRAF 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRAF 1 , TRAF 2 , and TRAF 3 share a conserved C terminal TRAF domain . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Association of TRAF 1 , TRAF 2 , and TRAF 3 with an Epstein Barr virus LMP 1 domain important for B lymphocyte transformation : role in NF kappaB activation . ^^^ We now show that in EBV transformed B lymphocytes most of TRAF 1 or TRAF 3 and 5 % of TRAF 2 are associated with LMP 1 and that most of LMP 1 is associated with TRAF 1 or TRAF 3 . ^^^ TRAF 1 , TRAF 2 , and TRAF 3 bind to a single site in the LMP 1 CT corresponding to amino acids ( aa ) 199 to 214 , within a domain which is important for B lymphocyte growth transformation ( aa 187 to 231 ) . ^^^ NF kappaB activation by LMP 1 ( 1 231 ) is likely to be mediated by TRAF1 / TRAF2 heteroaggregates since TRAF 1 is unique among the TRAFs in coactivating NF kappaB with LMP 1 ( 1 231 ) , a TRAF 2 dominant negative mutant can block LMP 1 ( 1 231 ) mediated NF kappaB activation as well as TRAF 1 coactivation , and 30 % of TRAF 2 is associated with TRAF 1 in EBV transformed B cells . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Induction of nuclear factor kappaB by the CD 30 receptor is mediated by TRAF 1 and TRAF 2 . ^^^ Coexpression of CD 30 with TRAF 1 or TRAF 2 but not TRAF 3 augmented NF kappaB activation through domain 2 but not domain 1 . ^^^ NF kappaB induction through domain 2 was inhibited by coexpression of either full length TRAF 3 or dominant negative forms of TRAF 1 or TRAF 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In this study , we report that two members of the TNFR associated factor ( TRAF ) family of proteins , TRAF 1 and TRAF 2 , independently bind to the intracellular domain of CD 30 ( CD30IC ) . ^^^ Transient overexpression of TRAF 2 , but not TRAF 1 , induced NF kappaB activation and HIV 1 long terminal repeat driven transcription in the T cell line , KT 3 . ^^^ Moreover , dominant negative mutants consisting of the TRAF domain of TRAF 1 and TRAF 2 inhibited CD 30 induction of NF kappaB activation and HIV 1 transcription . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor receptor ( TNFR ) associated factor 2 ( TRAF 2 ) and TRAF 1 were found as components of the TNFR 2 signaling complex , which exerts multiple biological effects on cells such as cell proliferation , cytokine production , and cell death . ^^^ In the TNFR 2 mediated signaling pathways , TRAF 2 works as a mediator for activation signals such as NF kappaB , but the role of TRAF 1 has not been previously determined . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Characterization of LMP 1 ' s association with TRAF 1 , TRAF 2 , and TRAF 3 . ^^^ We have characterized and mapped in detail a region of LMP 1 that associates with TRAF 1 , TRAF 2 , and TRAF 3 . ^^^ TRAF 1 , TRAF 2 , and TRAF 3 appear to associate independently with LMP 1 but bind an overlapping target site . ^^^ TRAF 3 associates with LMP 1 most avidly and can compete with TRAF 1 and TRAF 2 for binding to LMP 1 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic region of HVEM bound to several members of the TNFR associated factor ( TRAF ) family , namely TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 5 , but not to TRAF 6 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Clones corresponding to genes coding for TRAF 1 , TRAF 2 , and TRAF 3 molecules , postulated to be involved in signalling via the TNF and CD 40 receptors , were isolated . ^^^ The more amino terminal motif , 558PHYPEQET565 , binds TRAF 2 and 3 , while the more carboxyl terminal motif , 576MLSVEEEG583 , binds TRAF 1 and 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Casper interacts with FADD , caspase 8 , caspase 3 , TRAF 1 , and TRAF 2 through distinct domains . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| During CD 30 signal transduction , we found that binding of TRAF 2 to the cytoplasmic domain of CD 30 results in the rapid depletion of TRAF 2 and the associated protein TRAF 1 by proteolysis . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| While 4 1BB bound TRAF 2 and TRAF 1 , Ox 40 interacted with TRAF 3 and TRAF 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| However , TRAF2A levels in transiently transfected COS 7 cells approach those of TRAF 2 upon coexpression with TRAF 1 and / or TRAF 2 , indicating that TRAF2A stability is regulated by the binding of other TRAF family proteins . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Mutation analysis showed that TRAF 1 , TRAF 2 , and TRAF 3 were associated with one of two runs of acidic residues found in the cytoplasmic domain of 4 1BB . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| This report analyzes the gene expression of bcl xs , bcl xl , bax alpha , bax beta , fadd , fas , fas ligand ( fas L ) , ice , TNF alpha , TNF beta , TNFR 1 , TNFR 2 , TRAF 1 , TRAF 2 , TRAF 3 , cIAP 2 , and tradd at the level of mRNA in the single Reed Sternberg cells and their variants . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Overexpression of TRAF 2 potently activates NF kappaB , although TRAF 2 did not induce expression of the EGFR either alone or in combination with TRAF 1 and TRAF 3 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In T cells , we find that association of TRAF 1 and TRAF 2 with 4 1BB requires 4 1BB cross linking . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| RIP 2 interacted with other members of the TNFR 1 signaling complex , including inhibitor of apoptosis protein cIAP 1 and with members of the TNFR associated factor ( TRAF ) family , specifically TRAF 1 , TRAF 5 , and TRAF 6 , but not with TRAF 2 , TRAF 3 , or TRAF 4 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| CARDIAK interacted with the TNFR associated factors TRAF 1 and TRAF 2 , and a dominant negative form of TRAF 2 inhibited CARDIAK induced NF kappa B activation . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| One site is similar to activated TNFRII in associating with TNFR associated factors TRAF 1 and TRAF 2 , and the second site is similar to TNFRI in associating with the TNFRI death domain interacting protein TRADD . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 6 , but not TRAF 4 or TRAF 5 , bound directly to the CD 40 cytoplasmic domain . ^^^ CD 40 interactions with TRAF 2 and TRAF 3 were stronger than the interactions with TRAF 1 and TRAF 6 . ^^^ TRAF 1 , TRAF 2 , and TRAF 3 interacted with the same region . ^^^ Using peptides with progressive deletions , a minimal TRAF 1 , TRAF 2 , and TRAF 3 binding region was mapped to the PVQET sequence in the CD 40 cytoplasmic domain . ^^^ These studies demonstrate that the CD 40 cytoplasmic domain contains two nonoverlapping TRAF binding regions and suggest that TRAF 1 , TRAF 2 , and TRAF 3 could bind competitively to one site . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NF kappaB antiapoptosis : induction of TRAF 1 and TRAF 2 and c IAP 1 and c IAP 2 to suppress caspase 8 activation . ^^^ TRAF 1 ( TNFR associated factor 1 ) , TRAF 2 , and the inhibitor of apoptosis ( IAP ) proteins c IAP 1 and c IAP 2 were identified as gene targets of NF kappaB transcriptional activity . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In epithelial cells , very little TRAF 1 is expressed , and only TRAF 2 , 3 , and 5 , are significantly complexed with LMP 1 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments suggested potential interactions between the cytoplasmic tail of TRANCE R with TRAF 1 , TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| This amino acid alignment contains the EEEG sequence , which is essential for interaction of CD 30 or CD 40 with TRAFs ( TRAF 1 and TRAF 2 , but not TRAF 3 ) , and also contains the PIQED sequence , which is similar to PXQXT that is known to be necessary for interaction of TNF receptor 2 and CD 30 with TRAFs ( TRAF 1 , 2 , and 3 ) . . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| AITR associates with TRAF 1 ( TNF receptor associated factor 1 ) , TRAF 2 , and TRAF 3 , and induces nuclear factor ( NF ) kappaB activation via TRAF 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The core binding site for TRAF 1 , TRAF 2 , and TRAF 3 in CD 40 could be minimally substituted . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In contrast to TRAF 2 and TRAF 3 , direct binding of TRAF 1 , TRAF 4 , TRAF 5 , or TRAF 6 to CD 40 was not detected . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Biochemical and biophysical analyses demonstrated that TRAF domains of TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 6 formed homo trimers in solution . ^^^ In surface plasmon resonance studies , a hierarchy of affinity of trimeric TRAFs for trimeric CD 40 was found to be TRAF 2 > TRAF 3 > > TRAF 1 and TRAF 6 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| A 20 also has been shown to associate with TRAF 1 and TRAF 2 . ^^^ A 20 did not affect the association of TRAF 1 with TRAF 2 but did displace TRAF 1 from the LMP 1 complex . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Interestingly , overexpression of TRAF 1 in HEK293T completely prevented NF kappaB activation induced by TNF , IL 1 , or overexpression of TRAF 2 or TRAF 6 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| LMP 1 spontaneously aggregates in the plasma membrane and enables two transformation effector sites ( TES 1 and TES 2 ) within the 200 amino acid cytoplasmic carboxyl terminus to constitutively engage the tumor necrosis factor receptor ( TNFR ) associated factors TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 5 and the TNFR associated death domain proteins TRADD and RIP , thereby activating NF kappaB and c Jun N terminal kinase ( JNK ) . ^^^ Mutant and wt LMP 1 proteins were also similar in their constitutive association with TRAF 1 , TRAF 2 , TRAF 3 , TRADD , and RIP . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Expression of transcripts for TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 5 , as demonstrated by RT PCR , was noted in leukemic blasts that express CD30v . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor ( TNF ) receptor ( TNFR ) associated factors 1 and 2 ( TRAF 1 and TRAF 2 ) and inhibitor of apoptosis proteins cIAP 1 ( MIHB ) and cIAP 2 ( MIHC ) were recently identified as proteins that associate with the TNF alpha receptors TNFRI ( p 55 ) and TNFRII ( p 75 ) and inhibit TNF alpha induced programmed cell death or apoptosis . ^^^ In the original reports , TRAF 1 expression , unlike the ubiquitous TRAF 2 , was restricted to specific tissues in the lung , spleen , and testis . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In normal tissue , expression of the known NF kappaB regulated antiapoptotic factors , TRAF 1 , TRAF 2 , c IAP 1 , and c IAP 2 , is most pronounced in outer epidermis . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| DTRAF 1 can also self associate and binds to human TRAF 1 , TRAF 2 , and TRAF 4 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRADD is specific for TRAF 1 and TRAF 2 , which ensures the recruitment of clAPs for the direct inhibition of caspase activation in the signaling complex . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The in vivo locations of TRAF 1 , TRAF 2 , TRAF 5 , and TRAF 6 were determined in human and mouse tissues by immunohistochemistry . ^^^ Dynamic regulation of TRAFs was observed in cultured PBLs , where anti CD 3 Abs , mitogenic lectins , and ILs induced marked increases in the steady state levels of TRAF 1 , TRAF 2 , TRAF 5 , and TRAF 6 . ^^^ TRAF 1 was also highly inducible by CD 40 ligand in cultured germinal center B cells , whereas TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 were relatively unchanged . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| We investigated the constitutive expression of TRAF 1 and TRAF 2 in Hodgkin and Reed Sternberg ( HRS ) cells from archived paraffin embedded tissues obtained from 21 patients diagnosed with classical Hodgkin ' s disease ( HD ) . ^^^ In a selective portion of cases , examination of HRS cells for Epstein Barr virus ( EBV ) encoded RNA was performed by in situ hybridization , and the results were compared with the magnitude of TRAF 1 and TRAF 2 staining . ^^^ Moderate to high constitutive expression of TRAF 1 and TRAF 2 were found in 19 of 21 and 20 of 21 cases of classical HD , respectively . ^^^ These data demonstrate that constitutive expression of TRAF 1 and TRAF 2 is a characteristic feature of HRS cells from both patient and cell line specimens . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Caspase cleaved TRAF 1 negatively regulates the antiapoptotic signals of TRAF 2 during TNF induced cell death . ^^^ Importantly , we demonstrate that the cleavage product of TRAF 1 coimmunoprecipitates with TRAF 2 that is released from the TNF R 1 complex in response to prolonged TNF treatment . ^^^ These results indicate that caspase dependent cleavage of TRAF 1 generates TRAF 1 c fragments that are able to bind TRAF 2 , and then sequester TRAF 2 from the TNF R 1 complex , rendering cells , at least in part , sensitive to TNF . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| MUL and USP 7 are capable of binding in vitro via their TDs to all of the previously identified TRAF family proteins ( TRAF 1 , TRAF 2 , TRAF 3 , TRAF 4 , TRAF 5 , and TRAF 6 ) , whereas the TD of SPOP interacts weakly with TRAF 1 and TRAF 6 only . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In order to determine whether TRAF 1 and also TRAF 2 were expressed at the protein level in HD and whether there was any relationship to EBV status , immunohistochemistry has been used to detect these proteins in a series of HD specimens . ^^^ Using a sequential labelling approach , co localization of LMP 1 with either TRAF 1 or TRAF 2 was also demonstrated in HRS cells from EBV positive tumours . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Previous evidence indicates that the combined transcriptional activation of TRAF 1 , TRAF 2 , IAP 1 , and IAP 2 is required to suppress cell death by tumor necrosis factor ( TNF ) . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Using this system we demonstrate that unlike DIABLO / Smac , other proteins which interact with mammalian IAPs ( TAB 1 , Zap 1 , Traf 1 and Traf 2 ) do not act to antagonise IAP mediated caspase inhibition . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Overexpression of either TRAF 1 , 3 or an dominant negative mutant of TRAF 1 , 3 inhibited the expression of EGFR , while TRAF 2 or negative mutants of TRAF 2 did slightly so . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Although c IAP 1 bound TRAF 2 and TRAF 1 in vitro , it ubiquitinated only TRAF 2 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRAF 1 is unique among TRAFs because it lacks a RING finger domain present in TRAF 2 6 that has been shown to be required for TRAF 2 and TRAF 6 mediated activities . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Regulation of the subcellular localization of tumor necrosis factor receptor associated factor ( TRAF ) 2 by TRAF 1 reveals mechanisms of TRAF 2 signaling . ^^^ Although TRAF 1 can displace TRAF 2 and CD 40 from raft fractions , it promotes the ability of TRAF 2 activate signaling over a sustained period of time . ^^^ TRAF 1 ( / ) dendritic cells show attenuated responses to secondary stimulation by TRAF 2 dependent factors and increased stimulus dependent TRAF 2 degradation . ^^^ These findings offer insights into the mechanism of TRAF 2 signaling and identify a physiological role for TRAF 1 as a regulator of the subcellular localization of TRAF2 . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRAF 1 and to a lesser extent TRAF 2 were overexpressed in B CLL lymphocytes as compared with normal CD 19 ( + ) B cells . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| It is proposed that similar adaptive ' hot spots ' are also present on the binding surfaces of TRAF 1 , TRAF 2 and TRAF5 . . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| A novel anti apoptotic mechanism of FLIP in the recruitment of RIP and TRAF 1 and TRAF 2 to activate ERK and NF kappaB has been defined . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In this study we demonstrate the ability of the TRAF binding domain of LMP 1 to signal on the JNK / AP 1 axis in a cell type dependent manner that critically involves TRAF 1 and TRAF 2 . ^^^ Thus , expression of this LMP 1 domain in TRAF 1 positive lymphoma cells promotes significant JNK activation , which is blocked by dominant negative TRAF 2 but not TRAF 5 . ^^^ The critical role of TRAF 1 in the regulation of TRAF 2 dependent JNK signaling is particular to the TRAF binding domain of LMP 1 , since a homologous region in the cytoplasmic tail of CD 40 or the TRADD interacting domain of LMP 1 signal on the JNK axis independently of TRAF 1 status . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| We found that CDDP treatment affected neither NF kappaB activity nor the expression levels of antiapoptotic proteins , including TRAF 1 , TRAF 2 , and cFLIP , in B 88 cells . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Here , we show that TRAF 1 differentially affects TRAF 2 recruitment and activation of NF kappaB by members of the TNF receptor family . ^^^ Our results suggest that TRAF 1 and TRAF 1 ( 164 416 ) exert their regulatory effects on receptor induced NF kappaB activation not only by modulation of TRAF 2 receptor interaction but especially TRAF 1 ( 164 416 ) also by directly targeting the IKK complex . . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| We carried out comparative in situ hybridization analysis of five Traf genes Traf 1 , Traf 2 , Traf 3 , Traf 4 and Traf 6 during murine odontogenesis from the formation of the epithelial thickening to the early bell stage . ^^^ Expression of Traf 1 , Traf 2 and Traf 6 were observed in the outer edges of the bud epithelium whereas Traf 3 was strongly expressed at the tip of the bud epithelium . ^^^ Traf 2 showed restricted expression in the internal enamel epithelium of the bell stage while expression of Traf 1 , Traf 3 , Traf 4 and Traf 6 were observed in both the internal and the external enamel epithelium . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Of the 6 members of the TRAF family , TRAF 1 , TRAF 2 , and TRAF 3 are most commonly associated with LMP 1 . ^^^ OBJECTIVE : To characterize the expression patterns of these proteins in PTLDs , we studied a series of well characterized cases for expression of LMP 1 , TRAF 1 , TRAF 2 , TRAF 3 , and NFkappaB by immunohistochemical analysis . ^^^ METHODS : A total of 27 specimens from 25 patients were analyzed for LMP 1 , TRAF 1 , TRAF 2 , TRAF 3 , and NFkappaB ( active form ) by immunohistochemical analysis . ^^^ TRAF 1 , TRAF 2 , and TRAF 3 expression did not correlate with either EBER or LMP 1 expression . ^^^ TRAF 2 , but not TRAF 1 or TRAF 3 , expression correlated with NFkappaB expression ( P = . 02 ) . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| The majority of cytoplasmic TRAF 2 GFP and , to a lesser extent , TRAF 3 GFP , but not TRAF 1 GFP or TRAF 4 GFP , translocated into CD 40 signaling complexes within a few minutes after CD 40 triggering with the CD 40 ligand . ^^^ An excess of TRAF 2 allowed recruitment of TRAF 1 GFP to sites of CD 40 signaling , whereas an excess of TRAF 1 abrogated the interaction of TRAF 2 and CD 40 . ^^^ Overexpression of TRAF 1 , however , had no effect on the interaction of TRADD and TRAF 2 , known to be important for tumor necrosis factor receptor 1 ( TNF R 1 ) mediated NF kappaB activation . ^^^ Because of the trimeric organization of TRAF proteins , we propose that the stoichiometry of TRAF 1 TRAF2 heteromeric complexes ( ( TRAF 2 ) 2 TRAF 1 versus TRAF 2 ( TRAF 1 ) 2 ) determines their capability to mediate CD 40 signaling but has no major effect on TNF signaling . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Discrete functions of TRAF 1 and TRAF 2 in Drosophila melanogaster mediated by c Jun N terminal kinase and NF kappaB dependent signaling pathways . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Cellular inhibitor of apoptosis protein 1 ( c IAP 1 ) can regulate apoptosis through its interaction with downstream TNF receptor effectors ( TRAF 1 and TRAF 2 ) , by binding to and inhibiting certain caspases , and by controlling the levels of specific proapoptotic stimuli ( e . g . , Smac / DIABLO ) within the cell . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Notably , our results demonstrated that the CTAR 2 region appears to inhibit the recruitment of TRAF 1 and TRAF 2 to membrane rafts by the CTAR 1 region . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Caspase 2 interacts with TRAF 1 , TRAF 2 , and RIP 1 . ^^^ |
|
| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| In the normal rat cortex , a portion of TNFR 1 was present in lipid raft microdomains , where it associated with the adaptor proteins TRADD ( TNF receptor associated death domain ) , TNF receptor associated factor 2 ( TRAF 2 ) , the Ser / Thr kinase RIP ( receptor interacting protein ) , TRAF 1 , and cIAP 1 ( cellular inhibitor of apoptosis protein 1 ) , forming a survival signaling complex . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Multiple survival factor genes , including cellular inhibitor of apoptosis protein ( c IAP 1 ) , c IAP 2 , TNF receptor associated factor 1 ( TRAF 1 ) , TRAF 2 , B cell leukemia / lymphoma 2 ( Bcl 2 ) , Bcl 10 , A 1 , and cellular Fas associated death domain ( FADD ) like interleukin 1beta converting enzyme like inhibitory protein ( c FLIP ) , were expressed in basal conditions in both cultured RPE cells and RPE cells in situ , whereas survivin was expressed only by cultured cells . ^^^ TNF alpha upregulated expression of TRAF 1 , TRAF 2 , c IAP 1 , c IAP 2 , c FLIP , and A 1 . ^^^ TRAF 1 , c FLIP , and to a lesser extent c IAP 2 protein levels were increased by TNF alpha in a time dependent manner , whereas c IAP 1 , survivin , Bcl 10 ( L ) , and TRAF 2 protein levels were not influenced by TNF alpha treatment at any time point tested . ^^^ Overexpression of mutant IkappaB blocked TNF alpha induced TRAF 1 , TRAF 2 , c IAP 1 , c IAP 2 , c FLIP , and A 1 gene expression and downregulated TRAF 1 protein levels . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Foamy cells positive for the DEC 205 marker also expressed high levels of TNFR associated factor 1 ( TRAF 1 ) , TRAF 2 , and TRAF 3 , markers associated with resistance to apoptosis . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 and TRAF 3 are required for induction of NF kappaB and associated cell survival , as well as Jun amino terminal kinase phosphorylation by vFLIP , whereas TRAF 1 , TRAF 5 and TRAF 6 are dispensable . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Furthermore , mTNF alpha upregulated the expression of TNF receptor associated factor ( TRAF ) 1 and failed to induce TRAF 1 and TRAF 2 membrane translocation , but led to cytoplasmic colocalization . ^^^ In contrast , sTNF alpha stimulated the expression of TRAF 1 and TRAF 2 , recruiting both molecules onto the cell membrane poststimulation . ^^^ These results suggest that the increased susceptibility of HL 60 cells to mTNF alpha may be due to the failure of TRAF 2 membrane translocation caused by the upregulation of TRAF 1 expression and formation of a TRAF1 / TRAF2 complex in the cytoplasm , thereby inhibiting NF kappaB activation and inducing apoptosis . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| Using a new method for gene targeting by homologous recombination in somatic cells , we produced and studied signaling by CD 40 and its viral oncogenic mimic , latent membrane protein 1 ( LMP 1 ) in mouse B cell lines lacking TRAF 1 , TRAF 2 , or both TRAFs . ^^^ In the absence of TRAF 1 , an increased amount of TRAF 2 was recruited to lipid rafts , and subsequently , more robust degradation of TRAF 2 and TRAF 3 was induced in response to CD 40 signaling . ^^^ Taken together , our findings indicate that TRAF 1 and TRAF 2 cooperate in CD 40 but not LMP 1 signaling and suggest that cellular levels of TRAF 1 may play an important role in modulating the degradation of TRAF 2 and TRAF 3 in response to signals from the TNFR superfamily . . ^^^ |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92844 and Q13077 |
Pubmed |
SVM Score :0.0 |
| NA |
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