| TNF receptor associated factor 1 ( TRAF 1 ) negatively regulates Toll / IL 1 receptor domain containing adaptor inducing IFN beta ( TRIF ) mediated signaling . ^^^ TNF receptor associated factor 1 ( TRAF 1 ) negatively regulates Toll / IL 1 receptor domain containing adaptor inducing IFN beta ( TRIF ) mediated signaling . ^^^ The TRAF C domain of TRAF 1 and the TIR domain of TRIF were responsible for their interaction . ^^^ Overexpression of TRAF 1 inhibited TRIF and TLR 3 mediated activation of NF kappaB , IFN stimulated response element and the IFN beta promoter . ^^^ Overexpression of TRIF caused caspase dependent cleavage of TRAF 1 . ^^^ |