| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Complex formation between junctin , triadin , calsequestrin , and the ryanodine receptor . ^^^ We recently purified and cloned a fourth component of this complex , junctin , which exhibits homology with triadin and is the major 125I calsequestrin binding protein detected in cardiac sarcoplasmic reticulum vesicles ( Jones , L . ^^^ By a combination of approaches including calsequestrin affinity chromatography , filter overlay , immunoprecipitation assays , and fusion protein binding analyses , we find that junctin binds directly to calsequestrin , triadin , and the ryanodine receptor . ^^^ It appears that junctin and triadin interact directly in the junctional sarcoplasmic reticulum membrane and stabilize a complex that anchors calsequestrin to the ryanodine receptor . ^^^ Taken together , these results suggest that junctin , calsequestrin , triadin , and the ryanodine receptor form a quaternary complex that may be required for normal operation of Ca2+ release . . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the proteins involved in the Ca2+ release cascade ( ryanodine receptor , junctin , and triadin ) were downregulated , whereas Ca2+ uptake proteins ( Ca2+ ATPase and phospholamban ) were unchanged or slightly increased . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Furthermore , there were increases in protein levels of SR Ca2+ ATPase , phospholamban , and calreticulin and decreases in FKBP 12 , without alterations in ryanodine receptor , junctin , and triadin levels in transgenic hearts . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| This change in Ca2+ spark amplitude distribution was not associated with any change in the density of ryanodine receptors , calsequestrin , junctin , triadin 1 , Ca2+ ATPase , or phospholamban . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| In dog , protein expression of triadin and junctin was lower in atrium vs ventricle . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Calsequestrin is a high capacity Ca ( 2+ ) binding protein in the junctional sarcoplasmic reticulum that forms a quaternary complex with junctin , triadin , and the ryanodine receptor . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Triadin is an integral membrane protein of sarcoplasmic reticulum shown to interact with the ryanodine receptor / Ca ( 2+ ) release channel , junctin , and calsequestrin . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Although the expression of calsequestrin , triadin , and phospholamban was not altered , we observed a progressive decrease in junctin abundance in beta 1 receptor transgenic mice ( Pbeta 1 adrenergic receptors . . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting identified the calsequestrin binding proteins of approximately 26 , 63 , 94 and 560 kDa as junctin , calsequestrin itself , triadin and the ryanodine receptor , respectively . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Overexpression of junctin led to down regulation of triadin and RyR but to up regulation of dihydropyridine receptor . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| As recently demonstrated by overlay assays using calsequestrin peroxidase conjugates , the major 63 kDa Ca ( 2+ ) binding protein of the sarcoplasmic reticulum forms complexes with itself , and with junctin ( 26 kDa ) , triadin ( 94 kDa ) and the ryanodine receptor ( 560 kDa ) [ Glover , L . , Culligan , K . , Cala , S . , Mulvey , C . & Ohlendieck , K . ( 2001 ) Biochim . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Triadin 1 is a protein in the cardiac junctional sarcoplasmic reticulum ( SR ) that interacts with the ryanodine receptor , junctin , and calsequestrin , proteins that are important for Ca ( 2+ ) release . ^^^ Triadin 1 overexpression was accompanied by time dependent changes in the protein expression of the ryanodine receptor , junctin , and cardiac / slow twitch muscle SR Ca ( 2+ ) ATPase isoform . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| A putative targeting mechanism of CS to jSR implies electrostatic interactions between negative charges on CS and positive charges on intraluminal domains of jSR integral proteins , such as triadin and junctin . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Junctin has recently been shown to bind directly to calsequestrin , the ryanodine receptor , and triadin . ^^^ These findings suggest that the putative transmembrane domain and subsequent initial portion of the putative luminal domain of junctin play an important role in the binding of junctin to calsequestrin , the ryanodine receptor , and triadin in the postnatal heart . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Triadin 1 is a major transmembrane protein in cardiac junctional sarcoplasmic reticulum ( SR ) , which forms a quaternary complex with the ryanodine receptor ( Ca ( 2+ ) release channel ) , junctin , and calsequestrin . ^^^ The levels of two other junctional SR proteins , the ryanodine receptor and junctin , were reduced by 55 % and 73 % , respectively , in association with triadin 1 overexpression , whereas the levels of calsequestrin , the Ca ( 2+ ) binding protein of junctional SR , and of phospholamban and SERCA2a , Ca ( 2+ ) handling proteins of the free SR , were unchanged . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Calsequestrin ( CSQ ) is a high capacity Ca ( 2+ ) binding protein in the junctional sarcoplasmic reticulum of striated muscles , and has been shown to regulate the ryanodine receptor ( RyR ) through triadin and junctin . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Junctin is a 26 kDa membrane protein that binds to calsequestrin , triadin , and ryanodine receptors ( RyRs ) within the junctional sarcoplasmic reticulum of calcium release units . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| In mammalian striated muscles , ryanodine receptor ( RyR ) , triadin , junctin , and calsequestrin form a quaternary complex in the lumen of sarcoplasmic reticulum . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Ca2+ release from the cardiac junctional sarcoplasmic reticulum ( SR ) is regulated by a complex of proteins , including the ryanodine receptor ( RyR ) , calsequestrin ( CSQ ) , junctin ( JCN ) , and triadin 1 ( TRD ) . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| The role of calsequestrin , triadin , and junctin in conferring cardiac ryanodine receptor responsiveness to luminal calcium . ^^^ In the present study , we investigated the potential role of the cardiac SR luminal auxiliary proteins calsequestrin ( CSQ ) , triadin 1 , and junctin in forming the luminal calcium sensor for the cardiac RyR . ^^^ When triadin 1 and junctin were added to the luminal side of purified channels , RyR Po increased significantly ; however , the channels still remained unresponsive to changes in luminal [ Ca ] . ^^^ In RyRs reassociated with triadin 1 and junctin , adding luminal CSQ produced a significant decrease in activity . ^^^ These results suggest that a complex of CSQ , triadin 1 , and junctin confer RyR luminal Ca sensitivity . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| This complex consists of the calcium release channel or ryanodine receptor ( RyR ) , the high capacity calcium binding protein calsequestrin located in the lumen of the junctional SR , and the junctional SR transmembrane proteins triadin 1 and junctin which are hypothesized to anchor calsequestrin to the RyR . ^^^ Transgenic mice with cardiac specific overexpression of triadin 1 or junctin show distinct cardiac phenotypes with altered cellular and subcellular morphology , changes in contractile properties and / or in the expression of junctional SR proteins suggesting that these junctional sarcoplasmic reticulum transmembrane proteins are of functional relevance for the regulation of calcium release in the heart . . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| In blot overlay assays , peroxidase conjugated calsequestrin specifically bound to the ryanodine receptor , triadin , calsequestrin itself , and junctin , illustrating that the biological binding affinities are retained in electrophoretically prepared muscle proteins . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Ca ( 2+ ) storage and release in muscle cells are controlled by a complex of junctional sarcoplasmic reticulum ( jSR ) proteins , that includes the calcium binding protein calsequestrin ( CSQ ) , the Ca ( 2+ ) release channel ( ryanodine receptor or RyR ) and two transmembrane proteins that bind to RyR : junctin ( JNC ) and triadin ( Tr ) . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Junctin is a major transmembrane protein in cardiac junctional sarcoplasmic reticulum , which forms a quaternary complex with the ryanodine receptor ( Ca ( 2+ ) release channel ) , triadin , and calsequestrin . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| The polymer is anchored at one end to ryanodine receptor ( RyR ) Ca2+ release channels either via the intrinsic membrane proteins triadin and junctin or by binding directly to the RyR . ^^^ When triadin and junctin are present , calsequestrin maximally inhibits the Ca2+ release channel when the free Ca2+ concentration in the SR lumen is 1mM . ^^^ In addition , calsequestrin activates purified RyRs lacking triadin and junctin . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| The release of Ca from the SR may be regulated by the ryanodine receptor , triadin , junctin , calsequestrin , and a histidine rich , Ca binding protein ( HRC ) . ^^^ Interestingly , HRC overexpresssion was accompanied by increased protein levels of junctin ( 1 . 4 fold ) and triadin ( 1 . 8 fold ) , whereas the protein levels of ryanodine receptor , calsequestrin , phospholamban , and sarco ( endo ) plasmic reticulum Ca ATPase remained unaltered . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Junctin is a transmembrane protein of the cardiac junctional sarcoplasmic reticulum ( SR ) that binds to the ryanodine receptor , calsequestrin , and triadin 1 . ^^^ We conclude that in 3 wk old atria , junctin overexpression was associated with a reduced expression of triadin 1 resulting in a higher SR Ca2+ load without changes in contractility or heart rate . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| This complex includes proteins that interact with the cytoplasmic part of the RyR directly or indirectly ( e . g . calmodulin ( CaM ) , FK 506 binding proteins , protein kinase A , Ca CaM dependent protein kinase , phosphatases 1 and 2A , mAKAP , spinophilin , PR 130 , sorcin , triadin , junctin , calsequestrin and Homer ) . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Although we found the two proteins to undergo similar glycosylation and phosphorylation , we discovered that Ca ( 2+ ) dependent binding of the D307H mutant to both triadin 1 and junctin was reduced by greater than 50 % compared to WT . ^^^ CONCLUSIONS : We conclude that the point mutation D307H leads to a profoundly altered conformation that no longer responds normally to Ca ( 2+ ) and fails to bind normally to triadin and junctin . . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Calsequestrin , the major calcium sequestering protein in the sarcoplasmic reticulum of muscle , forms a quaternary complex with the ryanodine receptor calcium release channel and the intrinsic membrane proteins triadin and junctin . ^^^ We provide electrophysiological and biochemical evidence that : 1 ) , luminal calcium concentration of > / =4 mM dissociates calsequestrin from junctional face membrane , whereas in the range of 1 3 mM calsequestrin remains attached ; 2 ) , the association with calsequestrin inhibits ryanodine receptor activity , but amplifies its response to changes in luminal calcium concentration ; and 3 ) , under physiological calcium conditions ( 1 mM ) , phosphorylation of calsequestrin does not alter its ability to inhibit native ryanodine receptor activity when the anchoring proteins triadin and junctin are present . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Triadin 1 ( TRD ) is an integral membrane protein that associates with the ryanodine receptor ( RyR 2 ) , calsequestrin ( CASQ 2 ) and junctin to form a macromolecular Ca signaling complex in the cardiac junctional sarcoplasmic reticulum ( SR ) . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Using transmission electron microscopy , this study followed the formation of CRUs and their accrual of four components : the L type channel dihydropyridine receptors ( DHPRs ) of plasmalemma / T tubules ; the RyRs of jSR ; triadin ( Tr ) and junctin ( JnC ) , two homologous components of the jSR membrane ; and calsequestrin ( CSQ ) , the internal calcium binding proteins . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| Expression of calsequestrin and triadin assessed by Western blotting was similar in the infant and adult groups , but junctin expression was considerably higher in the adult groups . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| In cardiac muscle , junctin forms a quaternary protein complex with the ryanodine receptor ( RyR ) , calsequestrin , and triadin 1 at the luminal face of the junctional sarcoplasmic reticulum ( jSR ) . ^^^ |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12797 and Q13061 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Complex formation between junctin , triadin , calsequestrin , and the ryanodine receptor . ^^^ We recently purified and cloned a fourth component of this complex , junctin , which exhibits homology with triadin and is the major 125I calsequestrin binding protein detected in cardiac sarcoplasmic reticulum vesicles ( Jones , L . ^^^ By a combination of approaches including calsequestrin affinity chromatography , filter overlay , immunoprecipitation assays , and fusion protein binding analyses , we find that junctin binds directly to calsequestrin , triadin , and the ryanodine receptor . ^^^ It appears that junctin and triadin interact directly in the junctional sarcoplasmic reticulum membrane and stabilize a complex that anchors calsequestrin to the ryanodine receptor . ^^^ Taken together , these results suggest that junctin , calsequestrin , triadin , and the ryanodine receptor form a quaternary complex that may be required for normal operation of Ca2+ release . . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the proteins involved in the Ca2+ release cascade ( ryanodine receptor , junctin , and triadin ) were downregulated , whereas Ca2+ uptake proteins ( Ca2+ ATPase and phospholamban ) were unchanged or slightly increased . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Furthermore , there were increases in protein levels of SR Ca2+ ATPase , phospholamban , and calreticulin and decreases in FKBP 12 , without alterations in ryanodine receptor , junctin , and triadin levels in transgenic hearts . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| This change in Ca2+ spark amplitude distribution was not associated with any change in the density of ryanodine receptors , calsequestrin , junctin , triadin 1 , Ca2+ ATPase , or phospholamban . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| In dog , protein expression of triadin and junctin was lower in atrium vs ventricle . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Calsequestrin is a high capacity Ca ( 2+ ) binding protein in the junctional sarcoplasmic reticulum that forms a quaternary complex with junctin , triadin , and the ryanodine receptor . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Triadin is an integral membrane protein of sarcoplasmic reticulum shown to interact with the ryanodine receptor / Ca ( 2+ ) release channel , junctin , and calsequestrin . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| A putative targeting mechanism of CS to jSR implies electrostatic interactions between negative charges on CS and positive charges on intraluminal domains of jSR integral proteins , such as triadin and junctin . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Junctin has recently been shown to bind directly to calsequestrin , the ryanodine receptor , and triadin . ^^^ These findings suggest that the putative transmembrane domain and subsequent initial portion of the putative luminal domain of junctin play an important role in the binding of junctin to calsequestrin , the ryanodine receptor , and triadin in the postnatal heart . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Triadin 1 is a major transmembrane protein in cardiac junctional sarcoplasmic reticulum ( SR ) , which forms a quaternary complex with the ryanodine receptor ( Ca ( 2+ ) release channel ) , junctin , and calsequestrin . ^^^ The levels of two other junctional SR proteins , the ryanodine receptor and junctin , were reduced by 55 % and 73 % , respectively , in association with triadin 1 overexpression , whereas the levels of calsequestrin , the Ca ( 2+ ) binding protein of junctional SR , and of phospholamban and SERCA2a , Ca ( 2+ ) handling proteins of the free SR , were unchanged . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Calsequestrin ( CSQ ) is a high capacity Ca ( 2+ ) binding protein in the junctional sarcoplasmic reticulum of striated muscles , and has been shown to regulate the ryanodine receptor ( RyR ) through triadin and junctin . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Junctin is a 26 kDa membrane protein that binds to calsequestrin , triadin , and ryanodine receptors ( RyRs ) within the junctional sarcoplasmic reticulum of calcium release units . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Although the expression of calsequestrin , triadin , and phospholamban was not altered , we observed a progressive decrease in junctin abundance in beta 1 receptor transgenic mice ( Pbeta 1 adrenergic receptors . . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting identified the calsequestrin binding proteins of approximately 26 , 63 , 94 and 560 kDa as junctin , calsequestrin itself , triadin and the ryanodine receptor , respectively . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Overexpression of junctin led to down regulation of triadin and RyR but to up regulation of dihydropyridine receptor . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| As recently demonstrated by overlay assays using calsequestrin peroxidase conjugates , the major 63 kDa Ca ( 2+ ) binding protein of the sarcoplasmic reticulum forms complexes with itself , and with junctin ( 26 kDa ) , triadin ( 94 kDa ) and the ryanodine receptor ( 560 kDa ) [ Glover , L . , Culligan , K . , Cala , S . , Mulvey , C . & Ohlendieck , K . ( 2001 ) Biochim . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Triadin 1 is a protein in the cardiac junctional sarcoplasmic reticulum ( SR ) that interacts with the ryanodine receptor , junctin , and calsequestrin , proteins that are important for Ca ( 2+ ) release . ^^^ Triadin 1 overexpression was accompanied by time dependent changes in the protein expression of the ryanodine receptor , junctin , and cardiac / slow twitch muscle SR Ca ( 2+ ) ATPase isoform . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| This complex consists of the calcium release channel or ryanodine receptor ( RyR ) , the high capacity calcium binding protein calsequestrin located in the lumen of the junctional SR , and the junctional SR transmembrane proteins triadin 1 and junctin which are hypothesized to anchor calsequestrin to the RyR . ^^^ Transgenic mice with cardiac specific overexpression of triadin 1 or junctin show distinct cardiac phenotypes with altered cellular and subcellular morphology , changes in contractile properties and / or in the expression of junctional SR proteins suggesting that these junctional sarcoplasmic reticulum transmembrane proteins are of functional relevance for the regulation of calcium release in the heart . . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| In blot overlay assays , peroxidase conjugated calsequestrin specifically bound to the ryanodine receptor , triadin , calsequestrin itself , and junctin , illustrating that the biological binding affinities are retained in electrophoretically prepared muscle proteins . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Ca ( 2+ ) storage and release in muscle cells are controlled by a complex of junctional sarcoplasmic reticulum ( jSR ) proteins , that includes the calcium binding protein calsequestrin ( CSQ ) , the Ca ( 2+ ) release channel ( ryanodine receptor or RyR ) and two transmembrane proteins that bind to RyR : junctin ( JNC ) and triadin ( Tr ) . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Junctin is a major transmembrane protein in cardiac junctional sarcoplasmic reticulum , which forms a quaternary complex with the ryanodine receptor ( Ca ( 2+ ) release channel ) , triadin , and calsequestrin . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| In mammalian striated muscles , ryanodine receptor ( RyR ) , triadin , junctin , and calsequestrin form a quaternary complex in the lumen of sarcoplasmic reticulum . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Ca2+ release from the cardiac junctional sarcoplasmic reticulum ( SR ) is regulated by a complex of proteins , including the ryanodine receptor ( RyR ) , calsequestrin ( CSQ ) , junctin ( JCN ) , and triadin 1 ( TRD ) . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| The role of calsequestrin , triadin , and junctin in conferring cardiac ryanodine receptor responsiveness to luminal calcium . ^^^ In the present study , we investigated the potential role of the cardiac SR luminal auxiliary proteins calsequestrin ( CSQ ) , triadin 1 , and junctin in forming the luminal calcium sensor for the cardiac RyR . ^^^ When triadin 1 and junctin were added to the luminal side of purified channels , RyR Po increased significantly ; however , the channels still remained unresponsive to changes in luminal [ Ca ] . ^^^ In RyRs reassociated with triadin 1 and junctin , adding luminal CSQ produced a significant decrease in activity . ^^^ These results suggest that a complex of CSQ , triadin 1 , and junctin confer RyR luminal Ca sensitivity . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| The polymer is anchored at one end to ryanodine receptor ( RyR ) Ca2+ release channels either via the intrinsic membrane proteins triadin and junctin or by binding directly to the RyR . ^^^ When triadin and junctin are present , calsequestrin maximally inhibits the Ca2+ release channel when the free Ca2+ concentration in the SR lumen is 1mM . ^^^ In addition , calsequestrin activates purified RyRs lacking triadin and junctin . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| The release of Ca from the SR may be regulated by the ryanodine receptor , triadin , junctin , calsequestrin , and a histidine rich , Ca binding protein ( HRC ) . ^^^ Interestingly , HRC overexpresssion was accompanied by increased protein levels of junctin ( 1 . 4 fold ) and triadin ( 1 . 8 fold ) , whereas the protein levels of ryanodine receptor , calsequestrin , phospholamban , and sarco ( endo ) plasmic reticulum Ca ATPase remained unaltered . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Junctin is a transmembrane protein of the cardiac junctional sarcoplasmic reticulum ( SR ) that binds to the ryanodine receptor , calsequestrin , and triadin 1 . ^^^ We conclude that in 3 wk old atria , junctin overexpression was associated with a reduced expression of triadin 1 resulting in a higher SR Ca2+ load without changes in contractility or heart rate . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| This complex includes proteins that interact with the cytoplasmic part of the RyR directly or indirectly ( e . g . calmodulin ( CaM ) , FK 506 binding proteins , protein kinase A , Ca CaM dependent protein kinase , phosphatases 1 and 2A , mAKAP , spinophilin , PR 130 , sorcin , triadin , junctin , calsequestrin and Homer ) . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Although we found the two proteins to undergo similar glycosylation and phosphorylation , we discovered that Ca ( 2+ ) dependent binding of the D307H mutant to both triadin 1 and junctin was reduced by greater than 50 % compared to WT . ^^^ CONCLUSIONS : We conclude that the point mutation D307H leads to a profoundly altered conformation that no longer responds normally to Ca ( 2+ ) and fails to bind normally to triadin and junctin . . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Calsequestrin , the major calcium sequestering protein in the sarcoplasmic reticulum of muscle , forms a quaternary complex with the ryanodine receptor calcium release channel and the intrinsic membrane proteins triadin and junctin . ^^^ We provide electrophysiological and biochemical evidence that : 1 ) , luminal calcium concentration of > / =4 mM dissociates calsequestrin from junctional face membrane , whereas in the range of 1 3 mM calsequestrin remains attached ; 2 ) , the association with calsequestrin inhibits ryanodine receptor activity , but amplifies its response to changes in luminal calcium concentration ; and 3 ) , under physiological calcium conditions ( 1 mM ) , phosphorylation of calsequestrin does not alter its ability to inhibit native ryanodine receptor activity when the anchoring proteins triadin and junctin are present . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Triadin 1 ( TRD ) is an integral membrane protein that associates with the ryanodine receptor ( RyR 2 ) , calsequestrin ( CASQ 2 ) and junctin to form a macromolecular Ca signaling complex in the cardiac junctional sarcoplasmic reticulum ( SR ) . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Using transmission electron microscopy , this study followed the formation of CRUs and their accrual of four components : the L type channel dihydropyridine receptors ( DHPRs ) of plasmalemma / T tubules ; the RyRs of jSR ; triadin ( Tr ) and junctin ( JnC ) , two homologous components of the jSR membrane ; and calsequestrin ( CSQ ) , the internal calcium binding proteins . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| Expression of calsequestrin and triadin assessed by Western blotting was similar in the infant and adult groups , but junctin expression was considerably higher in the adult groups . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| In cardiac muscle , junctin forms a quaternary protein complex with the ryanodine receptor ( RyR ) , calsequestrin , and triadin 1 at the luminal face of the junctional sarcoplasmic reticulum ( jSR ) . ^^^ |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13061 and Q12797 |
Pubmed |
SVM Score :0.0 |
| NA |
|