| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| Using the two hybrid system , we show that RalGDS RBD interacts with H ras , K ras , and Rap , and with active but not with inactive point mutants of these Ras like GTPases . ^^^ These data indicate that RalGDS is a putative effector molecule for R ras , H ras , K ras , and Rap . . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| This new GDS , ralGDS , is at least 20 fold more active on the ralA and ralB GTPases than on any other GTPase tested , including other members of the ras family ( H ras , N ras , K ras , R ras , rap1a and rap 2 ) , members of the rho family ( rhoA , rhoB and CDC 42 Hs ) and members of the rab family ( rab3a and ypt 1 ) . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| We examined the effects of the Gly 60 to Ala mutation on the interaction of H Ras with Ras GTPase activating protein ( GAP ) , neurofibromin 1 ( NF 1 ) , Raf 1 , and ral guanine nucleotide dissociation stimulator ( ralGDS ) , factors that interact with GTP bound form of H Ras . ^^^ The G60A mutation moderately reduces the binding of H Ras to Raf 1 Ras binding domain ; however , the binding of H Ras to ralGDS Ras binding domain was more significantly affected by the same mutation . ^^^ These results indicate that although GAP , NF 1 , Raf 1 , and ralGDS all interact with H Ras in a GTP dependent manner and they are able to compete against each other for binding to H Ras , these factors share overlapping but not identical binding domains on H Ras . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| Here we report that a non Raf binding Ras effector loop variant ( H Ras G12V , E37G ) , which retains interaction with the Ral guanine nucleotide dissociation stimulator ( RalGDS ) , inhibits the conversion of MyoD expressing C3H10T1 / 2 mouse fibroblasts to skeletal muscle . ^^^ We show that H Ras G12V , E37G , RalGDS , and the membrane localized RalGDS CAAX protein inhibit the activity of alpha actin Luc , a muscle specific reporter gene containing a necessary E box and serum response factor ( SRF ) binding site , while a RalGDS protein defective for Ras interaction has no effect on alpha actin Luc transcription . ^^^ Interestingly , RalGDS , RalGDS CAAX , and RalA G23V inhibit H Ras G12V , E37G induced expression of an SRF regulated reporter gene , demonstrating that signaling through RalGDS does not duplicate the action of H Ras G12V , E37G in this system . ^^^ As additional evidence for this , we show that H Ras G12V , E37G inhibits the expression of troponin 1 Luc , an SRF independent muscle specific reporter gene , whereas RalGDS and RalGDS CAAX do not . ^^^ Although our studies show that signaling through RalGDS can interfere with the expression of reporter genes dependent on SRF activity ( including alpha actin Luc ) , our studies also provide strong evidence that an additional signaling molecule ( s ) activated by H Ras G12V , E37G is required to achieve the complete inhibition of the myogenic differentiation program . . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| Neither Ha Ras ( G12V , T35S ) ( Ha RasV12S35 ) , which activates the Rafl signaling pathway , nor Ha Ras ( G12V , E37G ) ( Ha RasV12G37 ) , which stimulates the RalGDS pathway , did not have significant effects on factor withdrawal apoptosis of myeloid cells . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| Only the RalGDS 37G N Ras protein protected the N Ras knockout cells from apoptosis and restored transient rather than sustained JNK activation . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| The transduction of the H Ras mutants that retain certain functions ( V12S35 , V12G37 , and V12C40 retain the ability to activate Raf or RalGDS or phosphatidylinositol 3 kinase , respectively ) as well as the activated or dominant negative mutants of RalA ( V 23 and N 28 , respectively ) has revealed that the activation of Ras RalGEFs Ral pathway was responsible for the attenuation of the G ( 2 ) arrest induced by ethyl metanesulfonate or doxorubicin . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| Our results show that the activation of a PI3K related enzyme is crucial for H Ras induced MPF activation , whereas the recruitment of either MAPK or RalGDS is not . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| The interactions of H Ras , R Ras , and Rap1A with the Ras binding domains ( RBD ) of the c Raf kinase and of the Ral guanine nucleotide exchange factor ( RGF ) was studied biochemically in solution . ^^^ Interestingly , whereas H Ras binds with high affinity ( KD = 20 nM ) to Raf RBD and with low affinity ( KD = 1 microM ) to RGF RBD , Rap1A shows the opposite behavior . ^^^ |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12967 and P01112 |
Pubmed |
SVM Score :0.0 |
| NA |
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