| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The recruitment of c IAP 1 or c IAP 2 to the TNFR 2 signaling complex requires a TRAF 2 TRAF1 heterocomplex . . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The tumor necrosis factor receptor 2 signal transducers TRAF 2 and c IAP 1 are components of the tumor necrosis factor receptor 1 signaling complex . ^^^ Here we show that the TNF R 2 signal transducers TRAF 2 and c IAP 1 are a part of the TNF R 1 signaling complex . ^^^ The recruitment of TRAF 2 and c IAP 1 to TNF R 1 is TNF dependent , is mediated by TRADD , and is independent of TNF R 2 . ^^^ These data establish the physiological involvement of TRAF 2 and c IAP 1 in TNF R 1 signaling and help provide a molecular explanation for both the overlapping and distinct signals generated by the two TNF receptors . . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NF kappaB antiapoptosis : induction of TRAF 1 and TRAF 2 and c IAP 1 and c IAP 2 to suppress caspase 8 activation . ^^^ TRAF 1 ( TNFR associated factor 1 ) , TRAF 2 , and the inhibitor of apoptosis ( IAP ) proteins c IAP 1 and c IAP 2 were identified as gene targets of NF kappaB transcriptional activity . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In normal tissue , expression of the known NF kappaB regulated antiapoptotic factors , TRAF 1 , TRAF 2 , c IAP 1 , and c IAP 2 , is most pronounced in outer epidermis . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Transiently expressed soluble trimeric CD 40 cytoplasmic domain was isolated as complexes that contained TRAF 2 , TRAF 3 , TRAF 5 , TRAF 6 , and the inhibitor of apoptosis protein ( c IAP 1 ) . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Individual CTL undergoing apoptosis exhibit a dramatic and concurrent : ( 1 ) positive staining with Annexin 5 and propidium iodide ; ( 2 ) transformation to a smaller cell size characteristic of apoptosis ; and ( 3 ) a nearly complete loss of Bcl 2 , c IAP 1 , and TRAF 2 . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TNF RII and c IAP 1 mediate ubiquitination and degradation of TRAF 2 . ^^^ Although c IAP 1 bound TRAF 2 and TRAF 1 in vitro , it ubiquitinated only TRAF 2 . ^^^ Expression of wild type c IAP 1 , but not an E 3 defective mutant , resulted in TRAF 2 ubiquitination and degradation . ^^^ Moreover , E 3 defective c IAP 1 prevented TNF alpha induced TRAF 2 degradation and inhibited apoptosis . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The first complex ( complex 1 ) is formed at the membrane by TNF R 1 , TRADD , RIP , TRAF 2 and c IAP 1 , while the second complex ( complex 2 ) , formed in the cytosol , predominantly contains FADD and pro caspases 8 / 10 but lacks TNF R 1 . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Cellular inhibitor of apoptosis protein 1 ( c IAP 1 ) can regulate apoptosis through its interaction with downstream TNF receptor effectors ( TRAF 1 and TRAF 2 ) , by binding to and inhibiting certain caspases , and by controlling the levels of specific proapoptotic stimuli ( e . g . , Smac / DIABLO ) within the cell . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The release of cytochrome c from mitochrondria , the activation of caspase 3 , 8 , and 9 , the cleavage of nuclear poly ( ADP ribose ) polymerase ( PARP ) , and the expression of TNF receptors ( TNF R1 / p55 and TNF R2 / p75 ) , TNF R associated factor 2 ( TRAF 2 ) , and cellular inhibitor of apoptosis 1 ( c IAP 1 ) were determined by immunoblotting . ^^^ BLM exposure had no marked effect on AM expression of TNF R 1 or caspase 8 activation , but significantly increased the expression of TNF R 2 that was accompanied by a rise in c IAP 1 and a decrease in TRAF 2 . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Transient transfection studies with wild type and E 3 defective c IAP 1 revealed that c IAP 2 is a direct target for c IAP 1 mediated ubiquitination and subsequent degradation , which are potentiated by the adaptor function of TRAF 2 . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Multiple survival factor genes , including cellular inhibitor of apoptosis protein ( c IAP 1 ) , c IAP 2 , TNF receptor associated factor 1 ( TRAF 1 ) , TRAF 2 , B cell leukemia / lymphoma 2 ( Bcl 2 ) , Bcl 10 , A 1 , and cellular Fas associated death domain ( FADD ) like interleukin 1beta converting enzyme like inhibitory protein ( c FLIP ) , were expressed in basal conditions in both cultured RPE cells and RPE cells in situ , whereas survivin was expressed only by cultured cells . ^^^ TNF alpha upregulated expression of TRAF 1 , TRAF 2 , c IAP 1 , c IAP 2 , c FLIP , and A 1 . ^^^ TRAF 1 , c FLIP , and to a lesser extent c IAP 2 protein levels were increased by TNF alpha in a time dependent manner , whereas c IAP 1 , survivin , Bcl 10 ( L ) , and TRAF 2 protein levels were not influenced by TNF alpha treatment at any time point tested . ^^^ Overexpression of mutant IkappaB blocked TNF alpha induced TRAF 1 , TRAF 2 , c IAP 1 , c IAP 2 , c FLIP , and A 1 gene expression and downregulated TRAF 1 protein levels . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Signaling through tumor necrosis factor receptor 2 ( TNF R 2 ) results in ubiquitination of TRAF 2 by the E 3 c IAP 1 . ^^^ Furthermore , Ubc 6 co localized with translocated TRAF2 / c IAP 1 in the ER associated compartment in vivo , and a catalytically inactive Ubc 6 mutant inhibited TNF alpha induced , TNF R 2 dependent TRAF 2 degradation . ^^^ These results indicate that upon TNF R 2 signaling , translocation of TRAF 2 and c IAP 1 to an ER associated , Ubc 6 containing perinuclear compartment is required for the ubiquitination of TRAF 2 by c IAP 1 . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 has previously been demonstrated to activate both transcription factor nuclear factor kappaB ( NFkappaB ) and the c Jun N terminal kinase / stress activated protein kinase ( JNK / SAPK ) pathway , which in turn stimulates transcription factor activating protein 1 ( AP 1 ) mainly via phosphorylation of the c Jun component . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Cellular resistance to TNF requires TNF receptor associated factor 2 ( TRAF 2 ) , which has been hypothesized to act mainly by mediating activation of the transcription factors nuclear factor kB ( NFkB ) and activator protein 1 ( AP 1 ) . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| T2BP , a novel TRAF 2 binding protein , can activate NF kappaB and AP 1 without TNF stimulation . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The AP 1 activation by TRAF 2 , TRAF 5 , and TRAF 6 was also greatly suppressed by the dominant negative TAK 1 . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Since vFLIP physically interacts with tumor necrosis factor receptor associated factor 2 ( TRAF 2 ) and TRAF 2 mediates activation of the jun NH ( 2 ) terminal kinase ( JNK ) / activation protein 1 ( AP 1 ) pathway , we hypothesized that vFLIP might also activate this pathway . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : Tumor necrosis factor alpha ( TNF alpha ) exerts apoptosis throughout an intracellular transduction pathway that involves the protein kinases TRAF 2 ( integration point of apoptotic and survival signals ) , signal regulating kinase ( ASK 1 ) ( pro apoptotic protein ) , mitogen activated protein kinase kinase 4 ( MEK 4 ) ( p 38 activator and metastasis suppressor gene ) , Jun N terminal kinase ( JNK ) ( stress mitogen activated protein kinase ) and the transcription factor activator protein 1 ( AP 1 ) . ^^^ RESULTS : In normal prostates immunoreactions to TRAF 2 , ASK 1 , MEK 4 and JNK were positive , while no immunoreaction to AP 1 was detected . ^^^ Although in benign prostatic hyperplasia the percent of immunostained specimens and intensity of immunoreactions to TRAF 2 , ASK 1 , MEK 4 and JNK decreased , the immunoreaction to AP 1 was positive in 27 . 3 % . ^^^ In most carcinomatous specimens the immune reaction was negative for all proteins of the TRAF 2 / AP 1 pathway . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The functional importance of TRAF 2 TRAF3 heterotrimerization was demonstrated by the finding that TRAF 3 inhibited spontaneous NF kappaB , but not AP 1 , activation induced by TRAF 2 . ^^^ Together , these results reveal a novel association between TRAF 2 and TRAF 3 that is mediated by unique portions of each protein and that specifically regulates activation of NF kappaB , but not AP 1 . . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Introduction of a TRAF 2 expression vector in CD 95 sensitive Colo 357 cells resulted in ( 1 ) resistance to CD 95 induced apoptosis ; ( 2 ) increased constitutive NF kappaB and AP 1 activity ; and ( 3 ) higher basal secretion of matrix metalloproteinases ( MMPs ) , urokinase type plasminogen activator ( uPA ) , and IL 8 , leading to increased invasiveness . ^^^ Stimulation of TRAF 2 overexpressing cells with CD 95 ligand led to induction of NF kappaB and AP 1 , enhanced IL 8 and uPA secretion , and a further increased invasiveness . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 mediates activation of the transcription factors NF kappaB and AP 1 by TNF . ^^^ |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In summary , insertion of sequence 962 to 868 from the TRAP gene into the U 3 region of the MMLV LTR confers RANK L induced retroviral gene expression via Fos family protein interaction at AP 1 sites . . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Presence of AP 1 or metalloprotease treatment showed an inhibitory effect when platelets were activated with a low concentration of thrombin or TRAP . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Interestingly , xFADD and xRIP 1 interacted with each other via their DDs , and the expression of a mutant xRIP 1 containing only the DD ( xRIP 1 DD ) repressed the xFADD induced activation of NF kappaB and AP 1 . xFADD and xRIP 1 synergistically induced the activation of AP 1 and NF kappaB , both of which were partially mediated by TRAF 2 ( tumour necrosis factor receptor associated factor 2 ) and TAK 1 ( transforming growth factor beta activated kinase 1 ) . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We found that siRNA targeting the TRAF 6 C terminal ( siTRAF6C ) receptor interaction domain specifically reduced only TRAF 6 protein expression , without affecting TRAF 2 or 5 levels , and substantially interfered with IL 1 induced NF kappaB and c Jun / AP 1 activation . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The C terminal region of TRUSS interacted with TNF receptor associated factor 2 ( TRAF 2 ) and co expression of dominant inhibitory TRAF 2 with TRUSS inhibited AP 1 activation , suggesting that TRUSS signaling occurs upstream of TRAF 2 . ^^^ These findings suggest that TRUSS activates JNK in a TRAF 2 dependent fashion and is involved in TNF alpha induced AP 1 activation via JNK kinases . . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| RIP 2 interacted with other members of the TNFR 1 signaling complex , including inhibitor of apoptosis protein cIAP 1 and with members of the TNFR associated factor ( TRAF ) family , specifically TRAF 1 , TRAF 5 , and TRAF 6 , but not with TRAF 2 , TRAF 3 , or TRAF 4 . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor ( TNF ) receptor ( TNFR ) associated factors 1 and 2 ( TRAF 1 and TRAF 2 ) and inhibitor of apoptosis proteins cIAP 1 ( MIHB ) and cIAP 2 ( MIHC ) were recently identified as proteins that associate with the TNF alpha receptors TNFRI ( p 55 ) and TNFRII ( p 75 ) and inhibit TNF alpha induced programmed cell death or apoptosis . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In addition , the expression of TRAF 2 and cIAP 1 , which are transcriptionally regulated by NF kappaB and function as anti apoptotic molecules through the interruption of caspase pathway , was also inhibited by 5 FU . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Life cell imaging and confocal microscopy revealed that both TNF R 1 and TNF R 2 recruited the anti apoptotic factor cIAP 1 in a TRAF 2 dependent manner . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Endogenous association of TRAF 2 , TRAF 3 , cIAP 1 , and Smac with lymphotoxin beta receptor reveals a novel mechanism of apoptosis . ^^^ Four of five proteins identified , TRAF 2 , TRAF 3 , cIAP 1 , and Smac , are reported here . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The inhibitor of apoptosis proteins cIAP 1 and cIAP 2 were also recruited by TRAF 2 to sites of CD 40 signaling . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 , cIAP 1 and cIAP 2 all have RING finger domains known to possess E 3 ubiquitin ligase activity , implying that ubiquitination may play an important role in the TNF signaling pathway . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In the normal rat cortex , a portion of TNFR 1 was present in lipid raft microdomains , where it associated with the adaptor proteins TRADD ( TNF receptor associated death domain ) , TNF receptor associated factor 2 ( TRAF 2 ) , the Ser / Thr kinase RIP ( receptor interacting protein ) , TRAF 1 , and cIAP 1 ( cellular inhibitor of apoptosis protein 1 ) , forming a survival signaling complex . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| HGF / SF induced the expression of several known NF kappaB target genes ( cIAP 1 ( cellular inhibitor of apoptosis 1 ) , cIAP 2 , and TRAF 2 ( TNF receptor associated factor 2 ) ) in an NF kappaB dependent manner ; HGF / SF blocked the inhibition of expression of these genes by ADR . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here , we compared the structural features of cIAP 1 responsible for its interactions with two known target proteins , TRAF 2 and SMAC . ^^^ The N terminal ( BIR 1 ) and C terminal ( BIR 3 ) BIR domains of cIAP 1 were determined to be necessary and sufficient for binding TRAF 2 and SMAC , respectively . ^^^ Using these mutants , cIAP 1 mediated ubiquitination of TRAF 2 and SMAC in vitro was determined to be correspondingly dependent on intact binding sites on BIR 1 and BIR 3 . ^^^ Because TRAF 2 regulates NF kappaB activation , the effects of cIAP 1 on TRAF 2 mediated induction of NF kappaB transcriptional activity were studied using reporter gene assays . ^^^ Expression of a fragment of cIAP 1 encompassing the three BIR domains ( but not full length cIAP 1 ) greatly enhanced TRAF 2 induced increases in NF kappaB activity , providing a convenient assay for monitoring BIR dependent effects of cIAP 1 on TRAF 2 in cells . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| As MIHB and MIHC were able to bind to the tumor necrosis factor receptor associated factors TRAF 1 and TRAF 2 in yeast two hybrid assays , these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE like proteases . . ^^^ |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13490 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|