| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The TNF R 2 associated factor TRAF 2 has been shown to mediate activation of NF kappa B by TNF R 2 and CD 40 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 is an intracellular signal transducing protein recruited to the TNFR 1 and TNFR 2 receptors following TNF stimulation . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TNF signal transduction is mediated by TRAF 2 which binds the TNF Receptor 2 ( TNFR 2 ) and activates NFkappaB . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The mutated form TNFR 2 ( 196ARG ) shows a reduction of inducible TRAF 2 recruitment upon TNF alpha stimulation . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The ability of TNFR 80 to cooperate with TNFR 60 could be mapped to the binding site for the TNF receptor associated factor 2 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The CAP 1 protein contains a C terminal domain that shares strong amino acid sequence homology with a unique domain found recently in two putative signal transducing proteins that bind to the TNF R 2 cytosolic tail , TRAF 1 and TRAF 2 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| A truncated derivative of TRAF 2 lacking an amino terminal RING finger domain was a dominant negative inhibitor of NF kappa B activation mediated by TNF R 2 and CD 40 . ^^^ Thus , TRAF 2 is a common mediator of TNF R 2 and CD 40 signaling . . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Our analysis indicates that TRAF 1 and TRAF 2 are associated with the cytoplasmic domain of TNF R 2 in a heterodimeric complex in which TRAF 2 contacts the receptor directly . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Interaction of TRAF 2 with TNF R 2 and TRADD requires sequences at the C terminus of the TRAF C domain , whereas interaction with the protein kinase receptor interacting protein 5 ( RIP ) occurs via sequences at the N terminus of the TRAF C domain . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here we show that the TNF R 2 signal transducers TRAF 2 and c IAP 1 are a part of the TNF R 1 signaling complex . ^^^ The recruitment of TRAF 2 and c IAP 1 to TNF R 1 is TNF dependent , is mediated by TRADD , and is independent of TNF R 2 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here we report the crystal structure of the TRAF domain of human TRAF 2 , both alone and in complex with a peptide from TNF receptor 2 ( TNF R 2 ) . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Apoptotic crosstalk of TNF receptors : TNF R 2 induces depletion of TRAF 2 and IAP proteins and accelerates TNF R 1 dependent activation of caspase 8 . ^^^ Life cell imaging and confocal microscopy revealed that both TNF R 1 and TNF R 2 recruited the anti apoptotic factor cIAP 1 in a TRAF 2 dependent manner . ^^^ Thus , TNF R 2 may compete with TNF R 1 for the recruitment of newly synthesized TRAF 2 bound anti apoptotic factors , thereby promoting the formation of a caspase 8 activating TNF R 1 complex . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| BLM exposure had no marked effect on AM expression of TNF R 1 or caspase 8 activation , but significantly increased the expression of TNF R 2 that was accompanied by a rise in c IAP 1 and a decrease in TRAF 2 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| To reveal signaling events mediated by TNF receptors ( TNF Rs ) in tendinocytes , we focused on four signaling factors , TNF R 1 , TNF R 2 , TNF R associated factor 2 ( TRAF 2 ) and nuclear factor kappa B ( NF kappaB ) , and investigated the distribution and production of these factors . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Signaling through tumor necrosis factor receptor 2 ( TNF R 2 ) results in ubiquitination of TRAF 2 by the E 3 c IAP 1 . ^^^ In this report , we confirm that TRAF 2 translocates to a Triton 10 100 ( TX ) insoluble compartment upon TNF R 2 engagement . ^^^ Furthermore , Ubc 6 co localized with translocated TRAF2 / c IAP 1 in the ER associated compartment in vivo , and a catalytically inactive Ubc 6 mutant inhibited TNF alpha induced , TNF R 2 dependent TRAF 2 degradation . ^^^ These results indicate that upon TNF R 2 signaling , translocation of TRAF 2 and c IAP 1 to an ER associated , Ubc 6 containing perinuclear compartment is required for the ubiquitination of TRAF 2 by c IAP 1 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor receptor 2 ( TNFR 2 ) signaling is negatively regulated by a novel , carboxyl terminal TNFR associated factor 2 ( TRAF 2 ) binding site . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Role of TNF receptor associated factor 2 in the activation of IgM secretion by CD 40 and CD120b . ^^^ Furthermore , although both CD 40 and CD120b can bind TRAF 2 , TRAF 2 dependent CD 40 signals can not substitute for TRAF 2 dependent CD120b signals in the activation of IgM secretion . ^^^ Our results indicate a potentially important role for CD120b in the activation of IgM secretion and that TRAF 2 is used by CD 40 and CD120b in distinct ways . . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Both CD 40 and CD120b bind TNF receptor associated factor 2 ( TRAF 2 ) upon ligand stimulation . ^^^ Role of tumor necrosis factor ( TNF ) receptor associated factor 2 ( TRAF 2 ) in distinct and overlapping CD 40 and TNF receptor 2 / CD120b mediated B lymphocyte activation . ^^^ Wild type and TRAF 2 deficient B cells expressing CD 40 or the hybrid molecule ( human ) CD 40 ( mouse ) CD120b were examined . ^^^ CD 40 and CD120b mediated IgM secretion were partly TRAF 2 dependent , but only CD 40 required TRAF 2 for c Jun N terminal kinase activation . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The TRAP identified in the present study shares considerable homology with , and may be the human homologue of , a mouse protein , TNF receptor associated factor 2 ( TRAF 2 ) , that binds mouse TNFR 2 . . ^^^ After in vitro transcription / translation and 35S labelling the TRAP was precipitated using a fusion protein consisting of glutathione S transferase and the intracellular domain of TNFR 2 ( TNFR 2IC ) , which showed that the two proteins directly interact in a mammalian cell free system and also that identification of the TRAP was not an artifact of the two hybrid system . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The c IAPs do not directly contact TNFR 2 , but rather associate with TRAF 1 and TRAF 2 through their N terminal BIR motif comprising domain . ^^^ The recruitment of c IAP 1 or c IAP 2 to the TNFR 2 signaling complex requires a TRAF 2 TRAF1 heterocomplex . . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor receptor ( TNFR ) associated factor 2 ( TRAF 2 ) and TRAF 1 were found as components of the TNFR 2 signaling complex , which exerts multiple biological effects on cells such as cell proliferation , cytokine production , and cell death . ^^^ In the TNFR 2 mediated signaling pathways , TRAF 2 works as a mediator for activation signals such as NF kappaB , but the role of TRAF 1 has not been previously determined . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor receptor ( TNFR ) associated factor 2A ( TRAF2A ) , a TRAF 2 splice variant with an extended RING finger domain that inhibits TNFR 2 mediated NF kappaB activation . ^^^ Also in contrast to TRAF 2 , TRAF2A is unable to stimulate NF kappaB activity when overexpressed in 293 cells and acts as a dominant inhibitor of TNFR 2 dependent NF kappaB activation . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| This report analyzes the gene expression of bcl xs , bcl xl , bax alpha , bax beta , fadd , fas , fas ligand ( fas L ) , ice , TNF alpha , TNF beta , TNFR 1 , TNFR 2 , TRAF 1 , TRAF 2 , TRAF 3 , cIAP 2 , and tradd at the level of mRNA in the single Reed Sternberg cells and their variants . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| One site is similar to activated TNFRII in associating with TNFR associated factors TRAF 1 and TRAF 2 , and the second site is similar to TNFRI in associating with the TNFRI death domain interacting protein TRADD . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of TNF receptors ( TNFRI and TNFRII ) and the adapter molecules , including TNFR associated death domain protein ( TRADD ) , TNFR associated factor 2 ( TRAF 2 ) , and receptor interacting protein ( RIP ) , were analyzed both at the protein level by flow cytometry or Western blotting , and at the mRNA level using quantitative PCR or Northern blotting in lymphocytes from aged and young subjects . ^^^ An increased constitutive expression of TNFRI and TRADD and decreased expression of TNFRII and TRAF 2 were observed in lymphocytes from aged as compared with young controls . ^^^ |
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| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor ( TNF ) receptor ( TNFR ) associated factors 1 and 2 ( TRAF 1 and TRAF 2 ) and inhibitor of apoptosis proteins cIAP 1 ( MIHB ) and cIAP 2 ( MIHC ) were recently identified as proteins that associate with the TNF alpha receptors TNFRI ( p 55 ) and TNFRII ( p 75 ) and inhibit TNF alpha induced programmed cell death or apoptosis . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Structural studies have revealed a trimeric nature of TRAF 2 and a symmetrical mode of receptor binding , suggesting the involvement of trivalent TNFR 2 receptor interaction in the signal transduction . ^^^ In this study , using isothermal titration calorimetry ( ITC ) , we report thermodynamic characterization of the interaction between TRAF 2 and monomeric peptide sequences from TNFR members , including TNFR 2 , CD 40 , CD 30 , Ox 40 , and 4 1BB , and the Epstein Barr virus ( EBV ) transforming protein , latent infection membrane protein 1 ( LMP 1 ) . ^^^ |
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| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Upon TNF treatment , the TRAF 2 caveolin 1 complex transiently associates with TRADD , and upon overexpression of TNFR 2 , the TRAF 2 caveolin 1 complex stably associates with and causes redistribution of this receptor as detected by confocal fluorescence microscopy . ^^^ In human embryonic kidney 293 cells , which have minimal endogenous expression of caveolin 1 , cotransfection of TRAF 2 and caveolin 1 results in spontaneous association of these proteins which can further associate with and redistribute transfected TNFR 2 molecules . ^^^ The association of caveolin 1 with TNFR 2 depends upon TRAF 2 . ^^^ |
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| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The C terminal 16 amino acid residues of TNFR 2 are critical for Etk association and activation , and this Etk binding and activating motif in TNFR 2 is not overlapped with the TNFR associated factor type 2 ( TRAF 2 ) binding sequence . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| When TNFalpha bound TNFR 2 interacts with TNFR associated factor 2 ( TRAF 2 ) , expression of survival / antiapoptotic genes is up regulated . ^^^ In the present study we determined the changes in localization of TNFalpha and TRAF 2 and their mRNAs and the expression of TNFR 2 in granulosa cells during follicular atresia in pig ovaries . ^^^ In atretic follicles , decreased or trace staining for TRAF 2 and its mRNA and decreased expression of TNFR 2 were observed in the granulosa layer , where many apoptotic cells were seen . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of mRNA for TNF receptors ( TNFR ) , including TNFRI and TNFRII , and the adapter molecules , such as the TNF receptor associated death domain protein ( TRADD ) , Fas associated death domain protein ( FADD ) , receptor interacting protein ( RIP ) and TNF receptor associated factor 2 ( TRAF 2 ) were analyzed by reverse transcriptase ( RT ) PCR in bone marrow samples from control , MDS and AML cases . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| This article details the use of green fluorescent protein ( GFP ) mutants cyan fluorescent protein ( CFP ) and yellow fluorescent protein ( YFP ) to measure the association of the signaling component TRAF 2 with the TNFR 2 receptor to illustrate the versatility of this methodology . . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 downregulation , which has been proposed as the mechanism by which TNFR 2 enhances TNFR 1 signaling , was observed in PC 60 cells , but the TNRF 1 signal was not modulated . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| INTRODUCTION : TNF receptor associated factor 2 ( TRAF 2 ) is a signal transducer for RANK and for two TNF receptor isotypes , TNFR 1 and TNFR 2 . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Microscopical analyses of TNFR 2 clustering and TNF receptor associated factor 2 recruitment at contact sites to adjacent cells revealed the formation of stable TNFR complexes by target bound , processed prodrug , resembling the increased signal capacity of natural , membrane expressed TNF . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of mRNA for TNF alpha receptors ( TNFR 1 and TNFR 2 ) and the adapter molecules , such as the TNF receptor associated death domain protein ( TRADD ) , Fas associated death domain protein ( FADD ) , receptor interacting protein ( RIP ) , and TNF receptor associated factor 2 ( TRAF 2 ) were analyzed by reverse transcriptase PCR ( RT PCR ) in PBMCs from control and RA cases . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Remarkably , TRAF 2 DN overexpression , which was found to inhibit the TNF dependent recruitment of endogenous wild type TRAF 2 to the TNF R 75 signaling complex , could neither block TNF R 55 or TNF R 75 induced NF kappaB activation nor granulocyte / macrophage CSF secretion . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In transfected cells , TTRAP inhibits in a dose dependent manner the transcriptional activation of a nuclear factor kappaB ( NF kappaB ) dependent reporter mediated by CD 40 , TNF R 75 or Phorbol 12 myristate 13 acetate ( PMA ) and to a lesser extent by TRAF 2 , TRAF 6 , TNF alpha , or interleukin 1beta ( IL 1beta ) . ^^^ |
|
| Interacting proteins: P20333 and Q12933 |
Pubmed |
SVM Score :0.0 |
| However , upon costimulation of TNFR 80 , which mediates activation of nuclear factor kappaB and the c Jun amino terminal kinase via TRAF 2 , TNF induced cell death is drastically enhanced in parental and TRAF 2 transfected , but not in TRAF 2 ( 87 501 ) transfected cells . ^^^ These data point to a critical role of TRAF 2 in the apoptotic TNFR cross talk , whereby the TNFR 80 dependent enhancement of TNFR 60 induced cell death is due to TNFR 80 mediated negative regulation of TRAF 2 function ( s ) . ^^^ An interference with TRAF 2 function was confirmed independently by analysis of c Jun amino terminal kinase activation via TNFR 60 upon prestimulation of TNFR 80 . ^^^ We propose that the apoptotic TNFR cross talk is based on TNFR 80 mediated abrogation of antiapoptotic TRAF 2 dependent signaling pathways initiated by TNFR 60 , but not Apo1 / Fas or the apoptotic TNF related apoptosis inducing ligand receptors . . ^^^ |
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