| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Isolation and characterization of death domain ( TNF RI , Fas , TRADD , FADD / MORT 1 , RIP ) and TRAF domain containing proteins ( TRAF 1 , TRAF 2 , TRAF 3 ) have partially bridged a large molecular gap within one of several signaling pathways which originate at the plasma membrane and terminate in the nucleus . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here we show that the TNF R 2 signal transducers TRAF 2 and c IAP 1 are a part of the TNF R 1 signaling complex . ^^^ The recruitment of TRAF 2 and c IAP 1 to TNF R 1 is TNF dependent , is mediated by TRADD , and is independent of TNF R 2 . ^^^ These data establish the physiological involvement of TRAF 2 and c IAP 1 in TNF R 1 signaling and help provide a molecular explanation for both the overlapping and distinct signals generated by the two TNF receptors . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Our findings show that : ( 1 ) TNF RII is able to activate two members of the MAP kinase family : extracellular regulated kinase ( ERK ) and c jun N terminal kinase ( JNK ) ; ( 2 ) TRAF 2 , a molecule that binds TNF RII and associates indirectly with TNF RI , is sufficient to activate JNK upon overexpression ; ( 3 ) dominant negative TRAF 2 blocks TNF alpha mediated JNK activation and ( 4 ) TRAF 2 signals the activation of JNK and NF kappaB through different pathways . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The TNF R 1 associated death domain protein interacts with the p 55 TNF R 1 cytoplasmic domain and recruits the Fas associated death domain protein ( which directly activates the apoptotic proteases ) , the protein kinase receptor interacting protein , and TNF receptor associated factor 2 ( TRAF 2 ) . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| One of the proteins that binds to these sites is TRAF 2 , a signal transduction molecule that is also utilized by TNFR 1 to mediate the activation of several downstream kinases and transcription factors . ^^^ Depletion of intracellular TRAF 2 and its coassociated proteins also increased the sensitivity of the cell to undergoing apoptosis during activation of death inducing receptors such as TNFR 1 . ^^^ Consistent with this hypothesis , expression of a dominant negative form of TRAF 2 was found to potentiate TNFR 1 mediated death . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 is an intracellular signal transducing protein recruited to the TNFR 1 and TNFR 2 receptors following TNF stimulation . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| This report analyzes the gene expression of bcl xs , bcl xl , bax alpha , bax beta , fadd , fas , fas ligand ( fas L ) , ice , TNF alpha , TNF beta , TNFR 1 , TNFR 2 , TRAF 1 , TRAF 2 , TRAF 3 , cIAP 2 , and tradd at the level of mRNA in the single Reed Sternberg cells and their variants . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| RIP 2 interacted with other members of the TNFR 1 signaling complex , including inhibitor of apoptosis protein cIAP 1 and with members of the TNFR associated factor ( TRAF ) family , specifically TRAF 1 , TRAF 5 , and TRAF 6 , but not with TRAF 2 , TRAF 3 , or TRAF 4 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We show that the human SPS 1 homologue germinal center kinase ( GCK ) can interact in vivo with the TNFR 1 signal transducer TNFR associated factor 2 ( TRAF 2 ) and with MAPK / ERK kinase kinase 1 ( MEKK 1 ) , a MAPK kinase kinase ( MAPKKK ) upstream of the SAPKs , thereby coupling TRAF 2 to the SAPKs . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Interaction of the p 55 tumor necrosis factor receptor 1 ( TNF R 1 ) associated signal transducer TRADD with FADD signals apoptosis , whereas the TNF receptor associated factor 2 protein ( TRAF 2 ) is required for activation of the nuclear transcription factor nuclear factor kappa B . ^^^ TRAF 2 was both sufficient and necessary for activation of SAPK by TNF R 1 ; conversely , expression of a dominant negative FADD mutant , which blocks apoptosis , did not interfere with SAPK activation . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We have used the yeast interaction trap to isolate a protein , MADD , that associates with the death domain of TNFR 1 through its own C terminal death domain . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Most important , binding of IL 15 to IL 15Ralpha successfully competes with the TNFR 1 complex for TRAF 2 binding , which may impede assembly of key adaptor proteins to the TNFR 1 complex , and induces IkappaBalpha phosphorylation . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 deficiency results in hyperactivity of certain TNFR 1 signals and impairment of CD 40 mediated responses . ^^^ Furthermore , we could enhance the survival of TRAF 2 deficient mice by eliminating either TNF or TNFR 1 . ^^^ These data demonstrate two important roles of TRAF 2 , one as a negative regulator of certain TNFR 1 signals and the other as a positive mediator of CD 40 signaling . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The distinct roles of TRAF 2 and RIP in IKK activation by TNF R 1 : TRAF 2 recruits IKK to TNF R 1 while RIP mediates IKK activation . ^^^ To understand the mechanism by which RIP and TRAF 2 regulate TNF induced activation of the transcription factor NF kappaB , we investigated their respective roles in TNF R 1 mediated IKK activation using both RIP / and TRAF 2 / fibroblasts . ^^^ We found that TNF R 1 mediated IKK activation requires both RIP and TRAF 2 proteins . ^^^ Although TRAF 2 or RIP can be independently recruited to the TNF R 1 complex , neither one of them alone is capable of transducing the TNF signal that leads to IKK activation . ^^^ Moreover , we demonstrated that IKK is recruited to the TNF R 1 complex through TRAF 2 upon TNF treatment and that IKK activation requires the presence of RIP in the same complex . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| These data suggest that TNFR 1 , through a TNF receptor associated factor 2 NF kappaB signaling pathway , mediates TNFalpha induced expression of ICAM 1 on ASM cells by involving a thapsigargin sensitive signaling pathway . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Although fully capable of transducing apoptotic signals , which depend on the adapter Fas associated death domain protein ( FADD ) and on the subsequent recruitment / activation of the apoptotic proteases , TNF R 1 usually does not kill cells ; this is due to the induction of a complex cytoprotective response that requires TNF receptor associated factor 2 ( TRAF 2 ) , a signal transducer that couples TNF R 1 to both nuclear factor kappaB ( NFkappaB ) dependent and NFkappaB independent transcriptional events implicated in induction of genes protecting from TNF cytotoxicity . ^^^ Although absolutely required for cytoprotection , TNF receptor associated factor 2 is not sufficient to protect cells from TNF , thus suggesting that it may act in concert with additional TNF R 1 complex components . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TNF treatment released SODD from TNF R 1 , permitting the recruitment of proteins such as TRADD and TRAF 2 to the active TNF R 1 signaling complex . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Although both LMP 1 and TNFR 1 interact with TRADD and TRAF 2 , the different topologies of the signaling complexes correlate with substantial differences between LMP 1 and TNFR 1 signal transduction to JNK1 . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| RIP 3 could be co immunoprecipitated with RIP , TRAF 2 and TNFR 1 in mammalian cells . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Apoptotic crosstalk of TNF receptors : TNF R 2 induces depletion of TRAF 2 and IAP proteins and accelerates TNF R 1 dependent activation of caspase 8 . ^^^ Life cell imaging and confocal microscopy revealed that both TNF R 1 and TNF R 2 recruited the anti apoptotic factor cIAP 1 in a TRAF 2 dependent manner . ^^^ Thus , TNF R 2 may compete with TNF R 1 for the recruitment of newly synthesized TRAF 2 bound anti apoptotic factors , thereby promoting the formation of a caspase 8 activating TNF R 1 complex . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Adenosine also suppressed NF kappaB dependent reporter gene expression activated by TNF or by overexpression of TNFR 1 , TRAF 2 , NIK , and p 65 subunit of NF kappaB . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In an in vitro binding assay , the intracellular domain of TNF R 1 bound TRADD , RIP , and TRAF 2 but did not bind FADD or caspase 8 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here we show that upon TNFalpha binding , TNFR 1 translocates to cholesterol and sphingolipid enriched membrane microdomains , termed lipid rafts , where it associates with the Ser / Thr kinase RIP and the adaptor proteins TRADD and TRAF 2 , forming a signaling complex . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Solution structure of N TRADD and characterization of the interaction of N TRADD and C TRAF 2 , a key step in the TNFR 1 signaling pathway . ^^^ The N terminal domain ( N TRADD ) promotes the recruitment of TRAF 2 to TNFR 1 by binding to the C terminal of TRAF 2 , leading to the activation of JNK / AP1 and NF kappa B . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Enhanced osteoclastogenesis is associated with high expression of otherwise TNF and RANKL induced mediators , including c Src , TRAF 2 , TRAF 6 , and MEKK 1 , levels of which were notably reduced in TNFr 1 knockouts . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NF kappaB dependent reporter gene expression activated by TNF , TNFR 1 , TNF receptor associated factor 2 , and NF kappaB inducing kinase was also abrogated by IFN alpha pretreatment . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In this study , we further examined the interaction of the core protein with the signaling molecules of TNFR 1 , including FADD , TRADD , and TRAF 2 , in a human embryonic kidney cell line , HEK 293 , that overexpresses the HCV core protein . ^^^ TRAF 2 recruitment to the TNFR 1 signaling complex was also disrupted by the core protein . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The fusion of the transmembrane domain of LMP 1 to signaling domains of CD 40 , TNF R 1 and Fas activates their signaling ; we demonstrate that a fusion of LMP 1 with CD 40 recruits TRAF 2 intracellularly . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The initial plasma membrane bound complex ( complex 1 ) consists of TNFR 1 , the adaptor TRADD , the kinase RIP 1 , and TRAF 2 and rapidly signals activation of NF kappa B . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation of TNF R 1 revealed that in response to H2O2 , the adapter proteins , TRADD and TRAF 2 , and JNK were recruited to the receptor . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : These results suggest that physiological shear stress is antiinflammatory by specifically inhibiting MAP kinase signaling and inhibiting TRAF 2 interaction with TNFR 1 . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The first complex ( complex 1 ) is formed at the membrane by TNF R 1 , TRADD , RIP , TRAF 2 and c IAP 1 , while the second complex ( complex 2 ) , formed in the cytosol , predominantly contains FADD and pro caspases 8 / 10 but lacks TNF R 1 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Overexpression of TRAF 1 , however , had no effect on the interaction of TRADD and TRAF 2 , known to be important for tumor necrosis factor receptor 1 ( TNF R 1 ) mediated NF kappaB activation . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The cytokine tumor necrosis factor alpha ( TNF alpha ) stimulates the NF kappaB , SAPK / JNK , and p 38 mitogen activated protein ( MAP ) kinase pathways by recruiting RIP 1 and TRAF 2 proteins to the tumor necrosis factor receptor 1 ( TNFR 1 ) . ^^^ Genetic studies have revealed that RIP 1 links the TNFR 1 to the IkappaB kinase ( IKK ) complex , whereas TRAF 2 couples the TNFR 1 to the SAPK / JNK cascade . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| SCH 66336 also inhibited the NF kappaB dependent reporter gene expression activated by TNF , TNFR 1 , TRADD , TRAF 2 , NIK , and IKK but not that activated by the p 65 subunit of NF kappaB . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| An important regulatory step in control of antiapoptotic signaling is the assembly of the TNFR 1 TNFR 1 associated death domain protein ( TRADD ) TNFR associated factor 2 ( TRAF 2 ) receptor interacting protein ( RIP ) complex that controls NF kappaB activation . ^^^ Inhibition of either delta PKC or PI 3 kinase decreased TNF mediated recruitment of RIP and TRAF 2 to TNFR 1 . ^^^ Thus delta PKC and PI 3 kinase are positive regulators of TNF mediated association of TRAF 2 and RIP with TNFR 1 . ^^^ These results suggest that delta PKC and PI 3 kinase regulate TNF antiapoptotic signaling at the level of the TNFR 1 through control of assembly of a TNFR 1 TRADD RIP TRAF 2 complex . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| When overexpressed , PIAS 3 inhibits NF kappaB dependent transcription induced by treatment with tumor necrosis factor alpha ( TNF alpha ) or interleukin 1beta or by overexpression of TNF family receptors such as RANK , TNFR 1 , and CD 30 or signal transducers of TNF receptor associated factors ( TRAFs ) , including TRAF 2 , TRAF 5 , and TRAF 6 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Among the components of the TNFR 1 complex are proteins that possess ubiquitin protein isopeptide ligase ( E 3 ) activities , such as TNFR 1 associated factor 2 ( TRAF 2 ) , cellular inhibitor of apoptosis proteins ( c IAPs ) namely , c IAP 1 and c IAP 2 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We also found that RIP and TRAF 2 form a complex upon H ( 2 ) O ( 2 ) exposure , but without the participation of TNFR 1 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TNF binding induces release of AIP 1 from TNFR 1 , resulting in cytoplasmic translocation and concomitant formation of an intracellular signaling complex comprised of TRADD , RIP 1 , TRAF 2 , and AIPl . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Analyzing cell lines expressing an internalization deficient receptor ( TNF R 1 DeltaTRID ) revealed that recruitment of RIP 1 and TRAF 2 to TNF R 1 occurred at the level of the plasma membrane . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| BLM exposure had no marked effect on AM expression of TNF R 1 or caspase 8 activation , but significantly increased the expression of TNF R 2 that was accompanied by a rise in c IAP 1 and a decrease in TRAF 2 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In the normal rat cortex , a portion of TNFR 1 was present in lipid raft microdomains , where it associated with the adaptor proteins TRADD ( TNF receptor associated death domain ) , TNF receptor associated factor 2 ( TRAF 2 ) , the Ser / Thr kinase RIP ( receptor interacting protein ) , TRAF 1 , and cIAP 1 ( cellular inhibitor of apoptosis protein 1 ) , forming a survival signaling complex . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| To reveal signaling events mediated by TNF receptors ( TNF Rs ) in tendinocytes , we focused on four signaling factors , TNF R 1 , TNF R 2 , TNF R associated factor 2 ( TRAF 2 ) and nuclear factor kappa B ( NF kappaB ) , and investigated the distribution and production of these factors . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 downregulation , which has been proposed as the mechanism by which TNFR 2 enhances TNFR 1 signaling , was observed in PC 60 cells , but the TNRF 1 signal was not modulated . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Evodiamine also inhibited the NF kappaB dependent reporter gene expression activated by TNF , TNFR 1 , TRADD , TRAF 2 , NIK , and IKK but not that activated by the p 65 subunit of NF kappaB . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Moreover , TRAF 2 is absent from TNFR 1 complexes under stress conditions even after TNF alpha treatment . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| INTRODUCTION : TNF receptor associated factor 2 ( TRAF 2 ) is a signal transducer for RANK and for two TNF receptor isotypes , TNFR 1 and TNFR 2 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Zerumbone also inhibited the NF kappaB dependent reporter gene expression activated by TNF , TNFR 1 , TRADD , TRAF 2 , NIK , and IKK but not that activated by the p 65 subunit of NF kappaB . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The analysed factors included TNF alpha , TNFR 1 , TNF receptor associated death domain ( TRADD ) , caspase 3 , caspase 8 , TNF receptor associated factor 2 ( TRAF 2 ) and receptor interactive protein ( RIP ) , all of which are involved in the TNF alpha / TNFR 1 signalling pathway mediated apoptosis . ^^^ The quantitative RT PCR indicated that the infected muscle tissues up regulate the expression of pro apoptosis genes ( TNF alpha , TNFR 1 and TRADD , caspase 3 and caspase 8 ) , and anti apoptosis genes ( TRAF 2 and RIP ) at the beginning of cyst formation . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NF kappaB dependent reporter gene transcription induced by TNF , TNFR 1 , TRADD , TRAF 2 , NIK , and IKK was also blocked by guggulsterone but without affecting p 65 mediated gene transcription . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NF kappaB dependent reporter gene expression was also suppressed by 4 HPR , as was NF kappaB reporter activity induced by TNFR 1 , TRADD , TRAF 2 , NIK , and IKK but not that induced by p 65 transfection . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In this study , we focused on four signaling factors , TNFalpha , TNF R 1 , TNFR associated factor 2 ( TRAF 2 ) and caspase 3 , in order to elucidate the signaling events in tendinocytes . ^^^ In the normal tendon , large amounts of TRAF 2 were found in tendinocytes , but the amounts of TNF R 1 were small . ^^^ TNF R 1 , which was only faintly detected in the normal tendon , was detected at a high level in the inflamed tendon , and the amounts of TRAF 2 and caspase 3 also increased . ^^^ TNFalpha mRNA was also expressed in the scar formed tendon , though it showed weak signals , and the expression levels of TNF R 1 , TRAF 2 and caspase 3 proteins were very low . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Furthermore , SAHA inhibited the NF kappaB dependent reporter gene expression activated by TNF , TNFR 1 , TRADD , TRAF 2 , NF kappaB inducing kinase , IkappaBalpha kinase , and the p 65 subunit of NF kappaB . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The suppression of NF kappaB activation correlated with sequential inhibition of the tumor necrosis factor ( TNF ) induced activation of IkappaBalpha kinase , IkappaBalpha phosphorylation , IkappaBalpha degradation , p 65 phosphorylation , p 65 nuclear translocation , and the NF kappaB dependent reporter gene expression activated by TNF , TNFR 1 , TRAF 2 , NIK , IKK beta , and the p 65 subunit of NF kappaB . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NF kappaB reporter activity induced by TNFR 1 , TNF receptor associated death domain , TRAF 2 , TAK 1 , NF kappaB inducing kinase , and IKKbeta was inhibited by indirubin but not that induced by p 65 transfection . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The generation of the anti apoptotic signal on binding of TNF alpha to the TNF receptor ( TNFR ) 1 has been shown to be associated with the recruitment of TRAF 2 to the TNFR 1 in a process that requires ubiquitination of TRAF 2 with lysine 63 linked polyubiquitin chains . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of mRNA for TNF alpha receptors ( TNFR 1 and TNFR 2 ) and the adapter molecules , such as the TNF receptor associated death domain protein ( TRADD ) , Fas associated death domain protein ( FADD ) , receptor interacting protein ( RIP ) , and TNF receptor associated factor 2 ( TRAF 2 ) were analyzed by reverse transcriptase PCR ( RT PCR ) in PBMCs from control and RA cases . ^^^ PBMCs of RA patients showed a significant increase in TNF alpha and TNFR 1 expression as compared with that from control subjects along with significantly increased constitutive expression of TRADD , RIP , and TRAF 2 mRNA . ^^^ These data suggested enhanced signaling by the TNFR 1 TRADD RIP TRAF 2 pathway and suppressed signaling by the TNFR 1 TRADD FADD pathway in PBMCs of RA patients . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The CAP 1 protein contains a C terminal domain that shares strong amino acid sequence homology with a unique domain found recently in two putative signal transducing proteins that bind to the TNF R 2 cytosolic tail , TRAF 1 and TRAF 2 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Signaling via the TNF R family member CD 40 has recently been shown to result in recruitment of TRAF 2 to plasma membrane detergent resistant microdomains ( lipid rafts ) as well as to subsequently initiate TRAF 2 degradation . ^^^ As TRAF 2 associates with most members of the TNF R family , we wished to determine how this degradation occurs . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| To examine the role of the TNF R superfamily signaling protein TRAF 2 in mature B cell development and NF kappaB activation , conditionally TRAF 2 deficient mice were produced . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| One site is similar to activated TNFRII in associating with TNFR associated factors TRAF 1 and TRAF 2 , and the second site is similar to TNFRI in associating with the TNFRI death domain interacting protein TRADD . ^^^ TNFRI has been recently shown to activate NF kappaB through association with TRADD , RIP , and TRAF 2 ; activation of the NF kappaB inducing kinase ( NIK ) ; activation of the IkappaB alpha kinases ( IKKalpha and IKKbeta ) ; and phosphorylation of IkappaB alpha . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of TNF receptors ( TNFRI and TNFRII ) and the adapter molecules , including TNFR associated death domain protein ( TRADD ) , TNFR associated factor 2 ( TRAF 2 ) , and receptor interacting protein ( RIP ) , were analyzed both at the protein level by flow cytometry or Western blotting , and at the mRNA level using quantitative PCR or Northern blotting in lymphocytes from aged and young subjects . ^^^ An increased constitutive expression of TNFRI and TRADD and decreased expression of TNFRII and TRAF 2 were observed in lymphocytes from aged as compared with young controls . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor ( TNF ) receptor ( TNFR ) associated factors 1 and 2 ( TRAF 1 and TRAF 2 ) and inhibitor of apoptosis proteins cIAP 1 ( MIHB ) and cIAP 2 ( MIHC ) were recently identified as proteins that associate with the TNF alpha receptors TNFRI ( p 55 ) and TNFRII ( p 75 ) and inhibit TNF alpha induced programmed cell death or apoptosis . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Moreover , the transducing molecule TRAF 2 co immunoprecipitates with IL 15Ralpha and may be deflected to TNFRI using anti IL 15 blocking mAbs and TNF alpha . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Remarkably , TRAF 2 DN overexpression , which was found to inhibit the TNF dependent recruitment of endogenous wild type TRAF 2 to the TNF R 75 signaling complex , could neither block TNF R 55 or TNF R 75 induced NF kappaB activation nor granulocyte / macrophage CSF secretion . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| At least four different mechanisms of regulation can be distinguished : ( 1 ) NF kappaB mediated induction of proteins of the TNF R complex ; ( 2 ) NF kappaB independent protection against apoptosis by the TNF R associating factor 2 ( TRAF 2 ) mediated recruitment of antiapoptotic proteins ; ( 3 ) dual activation of apoptosis and NF kappaB by a single molecule ; and ( 4 ) amplification of the death signal by proteolytic inactivation of signaling proteins that are involved in NF kappaB activation or cell survival . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| By combined use of the yeast interaction trap system and a peptide scanning library , the novel WD repeat protein FAN has been identified , which specifically binds to a cytoplasmic nine amino acid binding motif of TNF R 55 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In addition , using the yeast interaction trap system , we have identified one protein ( FAN ) that binds to the NSD and mediates activation of N SMase as well as seventeen other potential interaction partners of TNF R 55 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The ability of TNFR 80 to cooperate with TNFR 60 could be mapped to the binding site for the TNF receptor associated factor 2 . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Overexpression of TRAF 2 and of a TRAF 2 mutant , deficient in nuclear factor kappaB activation , selectively desensitized and enhanced , respectively , TNFR 60 induced cell death in HeLa cells . ^^^ These data point to a critical role of TRAF 2 in the apoptotic TNFR cross talk , whereby the TNFR 80 dependent enhancement of TNFR 60 induced cell death is due to TNFR 80 mediated negative regulation of TRAF 2 function ( s ) . ^^^ An interference with TRAF 2 function was confirmed independently by analysis of c Jun amino terminal kinase activation via TNFR 60 upon prestimulation of TNFR 80 . ^^^ We propose that the apoptotic TNFR cross talk is based on TNFR 80 mediated abrogation of antiapoptotic TRAF 2 dependent signaling pathways initiated by TNFR 60 , but not Apo1 / Fas or the apoptotic TNF related apoptosis inducing ligand receptors . . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Cytoplasmic interaction of TNFRI with TRAF 2 and RIP allows for the activation of JNK and NFkappaB pathways . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of mRNA for TNF receptors ( TNFR ) , including TNFRI and TNFRII , and the adapter molecules , such as the TNF receptor associated death domain protein ( TRADD ) , Fas associated death domain protein ( FADD ) , receptor interacting protein ( RIP ) and TNF receptor associated factor 2 ( TRAF 2 ) were analyzed by reverse transcriptase ( RT ) PCR in bone marrow samples from control , MDS and AML cases . ^^^ These data suggested enhanced signaling by the TNFRI TRADD FADD pathway and suppressed signaling by the TRAF 2 pathway in RA . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Although both DR 3 and TNFRI activated TRAF 2 and NF kappaB to induce IL 8 gene transcription , the kinase cascades that transduce signals for DR 3 and TNFRI induced IL 8 gene transcription are different . ^^^ |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19438 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|