| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| These results show TRAF 5 is functionally similar to TRAF 2 in that both mediate activation NF kappaB and implicate TRAF 5 as a signal transducer for LT betaR . . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In vitro binding assays revealed that TRAF 5 associates with the cytoplasmic tail of CD 40 , but not with the cytoplasmic tail of tumor receptor factor receptor type 2 , which associates with TRAF 2 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Deletion analysis showed that residues 246 269 of CD 40 which are required for its association with TRAF 2 , TRAF 3 , and TRAF 5 are dispensable for its interaction with TRAF 6 , whereas residues 230 245 were required . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The two conserved subdomains , D 1 and D 2 , in the C terminal cytoplasmic region of CD 30 were tested for interaction with two tumor necrosis factor receptor associated factor ( TRAF ) proteins with NFkappaB activating capacity , TRAF 2 and TRAF 5 . ^^^ TRAF 5 , as well as TRAF 2 , interacted with the D 2 subdomain of CD 30 in vitro and in vivo . ^^^ Deletion analysis by the yeast two hybrid system revealed that the C terminal 22 and 30 amino acid residues are dispensable for interaction of TRAF 5 and TRAF 2 with CD 30 , respectively . ^^^ Overexpression of the TRAF domain of TRAF 2 or TRAF 5 showed a dominant negative effect on CD 30 mediated NFkappaB activation . ^^^ Expression of TRAF 2 and TRAF 5 mRNA was demonstrated in T and B cell lines that express CD 30 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| ATAR , a novel tumor necrosis factor receptor family member , signals through TRAF 2 and TRAF 5 . ^^^ The intracellular domains of human and mouse ATAR share only 25 % identity , yet both interact with TRAF 5 and TRAF 2 . ^^^ Co expression of ATAR with TRAF 5 , but not TRAF 2 , results in synergistic activation of NF kappaB , suggesting potentially different roles for TRAF 2 and TRAF 5 in post receptor signaling . . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic region of HVEM bound to several members of the TNFR associated factor ( TRAF ) family , namely TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 5 , but not to TRAF 6 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The site also binds TRAF 2 and TRAF 5 , but not TRAF 6 . ^^^ Mutation of critical residues in the TRAF 3 binding site of LMP 1 that prevents binding of TRAF 2 , TRAF 3 , and TRAF 5 does not affect NF kappaB activating potential . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| A threonine residue at position 234 , previously shown to be important for CD 40 association with TNF receptor associated factor 2 ( TRAF 2 ) , TRAF 3 , and TRAF 5 , was not required for NF kappa B activation . ^^^ This suggests that in B cells , CD 40 induced NF kappa B activation can occur independently of TRAF 2 and TRAF 5 association . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In vitro binding experiments showed that tumor necrosis factor receptor associated factor ( TRAF ) 1 , TRAF 2 , TRAF 3 , and TRAF 5 but not TRAF 4 associated with glutathione S transferase OX 40 fusion protein . ^^^ The cotransfection experiments using human embryo kidney cell derived HEK 293T cells showed that TRAF 2 , TRAF 3 , and TRAF 5 associated with OX 40 in vivo . ^^^ The introduction of the dominant negative mutants of TRAF 2 and TRAF 5 into HSB 2 OX 40 cells suppressed NF kappaB activation in a dose dependent manner . ^^^ In addition , the introduction of TRAF 3 together with the dominant negative mutants of TRAF 2 or TRAF 5 further reduced NF kappaB activation . ^^^ These results indicate that the NF kappaB activation resulting from OX 40 stimulation is mediated by both TRAF 2 and TRAF 5 , and is likely to be negatively modulated by TRAF3 . . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| CD 27 , a member of the tumor necrosis factor receptor superfamily , activates NF kappaB and stress activated protein kinase / c Jun N terminal kinase via TRAF 2 , TRAF 5 , and NF kappaB inducing kinase . ^^^ Co transfection of a dominant negative TRAF 2 or TRAF 5 blocked NF kappaB and SAPK / JNK activation induced by CD 27 . ^^^ A kinase inactive mutant NIK blocked CD 27 , TRAF 2 , and TRAF 5 mediated NF kappaB and SAPK / JNK activation . ^^^ These results indicate that TRAF 2 and TRAF 5 are involved in NF kappaB and SAPK / JNK activation by CD 27 , and NIK is a common downstream kinase of TRAF 2 and TRAF 5 for NF kappaB and SAPK / JNK activation . . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| RIP 2 interacted with other members of the TNFR 1 signaling complex , including inhibitor of apoptosis protein cIAP 1 and with members of the TNFR associated factor ( TRAF ) family , specifically TRAF 1 , TRAF 5 , and TRAF 6 , but not with TRAF 2 , TRAF 3 , or TRAF 4 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| From this deletion analysis , we determined that TRAF 2 , TRAF 5 , and TRAF 6 interact with RANK at its C terminal 85 amino acid tail ; the binding affinity appeared to be in the order of TRAF 2 > TRAF 5 > TRAF 6 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 6 , but not TRAF 4 or TRAF 5 , bound directly to the CD 40 cytoplasmic domain . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments suggested potential interactions between the cytoplasmic tail of TRANCE R with TRAF 1 , TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 . ^^^ Dominant negative forms of TRAF 2 , TRAF 5 , and TRAF 6 and an endogenous inhibitor of TRAF 2 , TRAF interacting protein ( TRIP ) , substantially inhibited TRANCE R mediated NF kappaB activation , suggesting a role of TRAFs in regulating DC and osteoclast function . ^^^ Analysis of TRANCE R deletion mutants suggested that the TRAF 2 and TRAF 5 interaction sites were restricted to the C terminal 93 amino acids ( C region ) . ^^^ TRAF 6 also complexed to the C region in addition to several regions N terminal to the TRAF 2 and TRAF 5 association sites . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here we show that ASK 1 interacts with members of the TRAF family and is activated by TRAF 2 , TRAF 5 , and TRAF 6 overexpression . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Although four members of the tumor necrosis factor receptor associated factor ( TRAF ) family , including TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 , bind to the CD 40 cyt , how each TRAF protein contributes to the NFkappaB activation by CD 40 is not clear . ^^^ Here we report that TRAF 2 , TRAF 3 , and TRAF 5 bind cyt C , whereas TRAF 6 binds cyt N . cyt N is conserved poorly between human and mouse CD 40 , while cyt C is highly conserved . ^^^ Furthermore , NFkappaB activation by cyt C is inhibited by a kinase negative form of NFkappaB inducing kinase more efficiently than that by cyt N , consistent with the result that NFkappaB activation by TRAF 2 and TRAF 5 is inhibited by a kinase negative form of NFkappaB inducing kinase more efficiently than that by TRAF 6 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Several tumor necrosis factor receptor associated factor ( TRAF ) family proteins including TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 , as well as Jak 3 , have been implicated as potential mediators of CD 40 signaling . ^^^ An extensive in vitro binding study indicated that TRAF 2 and TRAF 3 bind to the CD 40 cytoplasmic tail ( CD40ct ) with much higher affinity than TRAF 5 and TRAF 6 and that TRAF 2 and TRAF 3 bind to different residues of the CD40ct . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that the cytoplasmic domain of receptor activator of NF kappaB ( RANK ) interacts with TRAF 2 , TRAF 5 , and TRAF 6 and that its overexpression activates NF kappaB and JNK pathways . ^^^ Through a detailed mutational analysis of the cytoplasmic domain of RANK , we demonstrate that TRAF 2 and TRAF 5 bind to consensus TRAF binding motifs located in the C terminus at positions 565 568 and 606 611 , respectively . ^^^ Furthermore , transfection experiments with RANK and its deletion mutants in human embryonic 293 cells revealed that the TRAF 6 binding region ( 340 358 ) , but not the TRAF 2 or TRAF 5 binding region , is necessary and sufficient for RANK induced NF kappaB activation . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| This is the same region of CD 40 required for binding the TNF receptor associated factor 2 ( TRAF 2 ) , TRAF 3 , and TRAF 5 adapter proteins . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In contrast to TRAF 2 and TRAF 3 , direct binding of TRAF 1 , TRAF 4 , TRAF 5 , or TRAF 6 to CD 40 was not detected . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Here we show that TANK synergized with TRAF 2 , TRAF 5 , and TRAF 6 but not with TRAF 3 in SAPK activation . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We report that TRAF 2 , TRAF 5 and TRAF 6 associate with apoptosis signal regulating kinase 1 ( ASK 1 ) , and a catalytically inactive ASK 1 mutant blocks stress activated protein kinase ( SAPK ) / Jun NH 2 terminal kinase ( JNK ) activation by these TRAFs . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| LMP 1 spontaneously aggregates in the plasma membrane and enables two transformation effector sites ( TES 1 and TES 2 ) within the 200 amino acid cytoplasmic carboxyl terminus to constitutively engage the tumor necrosis factor receptor ( TNFR ) associated factors TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 5 and the TNFR associated death domain proteins TRADD and RIP , thereby activating NF kappaB and c Jun N terminal kinase ( JNK ) . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Expression of transcripts for TRAF 1 , TRAF 2 , TRAF 3 , and TRAF 5 , as demonstrated by RT PCR , was noted in leukemic blasts that express CD30v . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Using CD 40 mutants with impairments in their ability to bind selected TNF receptor associated factor ( TRAF ) family proteins , we observed that CD 40 mediated transcriptional induction of the germ line Ig Cgamma 1 and Ig Cepsilon promoters was markedly reduced by mutations that prevent TRAF 2 , TRAF 3 , TRAF 5 or TRAF 6 binding . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Consistent with this we show that p 62 selectively interacts with the TRAF domain of TRAF 6 but not that of TRAF 5 or TRAF 2 in co transfection experiments . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| In fact , activation of nuclear factor kappaB was induced by the overexpression of TROY and inhibited by dominant negative forms of TRAF 2 , TRAF 5 , and TRAF 6 , indicating that TRAFs and nuclear factor kappaB are involved in the signal transduction of TROY . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The in vivo locations of TRAF 1 , TRAF 2 , TRAF 5 , and TRAF 6 were determined in human and mouse tissues by immunohistochemistry . ^^^ Dynamic regulation of TRAFs was observed in cultured PBLs , where anti CD 3 Abs , mitogenic lectins , and ILs induced marked increases in the steady state levels of TRAF 1 , TRAF 2 , TRAF 5 , and TRAF 6 . ^^^ TRAF 1 was also highly inducible by CD 40 ligand in cultured germinal center B cells , whereas TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 were relatively unchanged . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Our data show that TRX treatment inhibits NF kappaB dependent transcription at the level of downstream of TRAFs and upstream of NIK : TRX inhibited TRAF 2 , TRAF 5 , and TRAF 6 induced NF kappaB activation but does not inhibit NIK , IKKalpha , and MEKK induced activation . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| VP8 * blocked JNK activation directed by TRAF 2 or TRAF 5 but had no effect on JNK activation directed by TRAF 6 or MEKK 1 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| MUL and USP 7 are capable of binding in vitro via their TDs to all of the previously identified TRAF family proteins ( TRAF 1 , TRAF 2 , TRAF 3 , TRAF 4 , TRAF 5 , and TRAF 6 ) , whereas the TD of SPOP interacts weakly with TRAF 1 and TRAF 6 only . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Critical roles of TRAF 2 and TRAF 5 in tumor necrosis factor induced NF kappa B activation and protection from cell death . ^^^ However , the exact roles of TRAF 2 and TRAF 5 in TNF induced NF kappaB activation still remain controversial . ^^^ To address this issue , we generated TRAF 2 and TRAF 5 double knockout ( DKO ) mice . ^^^ Collectively , these results indicate that both TRAF 2 and TRAF 5 are involved in TNF induced NF kappaB activation and protection from cell death . . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Transiently expressed soluble trimeric CD 40 cytoplasmic domain was isolated as complexes that contained TRAF 2 , TRAF 3 , TRAF 5 , TRAF 6 , and the inhibitor of apoptosis protein ( c IAP 1 ) . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF proteins are thought to be important regulators of cell death and cellular responses to stress , and TRAF 2 , TRAF 5 and TRAF 6 have been demonstrated to mediate activation of NF kappaB and JNK . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We report that in H RS cells overexpression of CD 30 leads to self aggregation , recruitment of TRAF 2 and TRAF 5 , and NF kappaB activation , independent of CD 30 ligand . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We previously showed that murine embryonic fibroblasts ( MEFs ) from TNF receptor associated factor 2 ( Traf 2 ) and Traf 5 double deficient ( double knockout ( DKO ) ) mice were highly susceptible to TNF induced cell death . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| The AP 1 activation by TRAF 2 , TRAF 5 , and TRAF 6 was also greatly suppressed by the dominant negative TAK 1 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Thus , expression of this LMP 1 domain in TRAF 1 positive lymphoma cells promotes significant JNK activation , which is blocked by dominant negative TRAF 2 but not TRAF 5 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Dominant negative forms of TRAF 2 and TRAF 5 substantially inhibited TweakR mediated NF kappa B activation , suggesting a role of TRAFs in regulating smooth muscle and endothelial cell function . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Finally , we show that fibroblast growth factor inducible 14 with a mutation at its TNF receptor associated factor ( TRAF ) binding site can not activate NF kappaB and that TWEAK fails to induce the p 100 processing and IkappaBalpha phosphorylation in cells deficient for TRAF 2 and TRAF 5 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor alpha induced IKK phosphorylation of NF kappaB p 65 on serine 536 is mediated through the TRAF 2 , TRAF 5 , and TAK 1 signaling pathway . ^^^ The TNF alpha but not IL 1beta induced Ser 536 phosphorylation was severely impaired in murine embryonic fibroblasts derived from traf 2 / traf 5 / mice . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| We now use cells lacking expression of TRAF 2 , TRAF 5 , TRAF 6 , IKKalpha , IKKbeta , IKKgamma , TAB 2 , IL 1 receptor associated kinase ( IRAK ) 1 , or IRAK 4 to assess their roles in LMP 1 mediated NF kappaB activation . ^^^ More surprisingly , NF kappaB responses were near normal in TRAF 2 and TRAF 5 double KO MEFs , IKKgamma KO MEFs , TAB 2 KO MEFs , and IRAK 4 KO MEFs but were highly deficient in TRAF 6 KO MEFs and IRAK 1 KO HEK 293 cells . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| Most importantly , we show that the binding between PKR and TRAFs is functionally relevant , as observed by the absence of NF kappa B activity upon PKR expression in cells genetically deficient in TRAF 2 and TRAF 5 or after expression of TRAF dominant negative molecules . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| When overexpressed , PIAS 3 inhibits NF kappaB dependent transcription induced by treatment with tumor necrosis factor alpha ( TNF alpha ) or interleukin 1beta or by overexpression of TNF family receptors such as RANK , TNFR 1 , and CD 30 or signal transducers of TNF receptor associated factors ( TRAFs ) , including TRAF 2 , TRAF 5 , and TRAF 6 . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| TRAF 2 and TRAF 3 are required for induction of NF kappaB and associated cell survival , as well as Jun amino terminal kinase phosphorylation by vFLIP , whereas TRAF 1 , TRAF 5 and TRAF 6 are dispensable . ^^^ |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00463 and Q12933 |
Pubmed |
SVM Score :0.0 |
| NA |
|