| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.97694013 |
| We also demonstrated that e3B1 associates with eps 8 in vivo . 0.97694013^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :1.1879303 |
| Eps 8 has been shown to form a Rac specific guanine nucleotide exchange factor complex with Abi 1 and Sos 1 , which seems essential for ruffling formation induced by oncogenic Ras . 1.1879303^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Recently , e3B1 , an eps 8 SH3 binding protein 1 , was also isolated as a human homolog to mouse Abi 1 . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| EPS 8 and E3B1 transduce signals from Ras to Rac . ^^^ Eps 8 ( relative molecular mass 97 , 000 ) is a substrate of receptors with tyrosine kinase activity which binds , through its SH 3 domain , to a protein designated E3b1 / Abi 1 . ^^^ Here we show that Eps 8 and E3b1 / Abi 1 participate in the transduction of signals from Ras to Rac , by regulating Rac specific guanine nucleotide exchange factor ( GEF ) activities . ^^^ We also show that Eps 8 , E3b1 and Sos 1 form a tri complex in vivo that exhibits Rac specific GEF activity in vitro . ^^^ We propose a model in which Eps 8 mediates the transfer of signals between Ras and Rac , by forming a complex with E3b1 and Sos 1 . . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| It possesses a Src homology 3 ( SH 3 ) motif , proline rich region , serine rich region and no catalytic domain , suggesting that it seems to be a signaling protein most similar to e3B1 , an eps 8 SH3 binding protein . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Thus , depending on its state of association with E3b1 or RN tre , Eps 8 participates in both EGFR signalling through Rac , and trafficking through Rab5 . . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| This is achieved through the formation of a tricomplex consisting of Eps 8 E3b1 Sos 1 , which is endowed with Rac guanine nucleotide exchange activity . ^^^ We propose a model whereby the engagement of Eps 8 in a tricomplex with E3b1 and Sos 1 facilitates the interaction of Eps 8 with Sos 1 and the consequent activation of an Sos 1 Rac specific catalytic ability . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Son of sevenless ( Sos 1 ) , a bifunctional guanine nucleotide exchange factor ( GEF ) , activates Ras in vivo and displays Rac GEF activity in vitro , when engaged in a tricomplex with Eps 8 and E3b1 Abi 1 , a RTK substrate and an adaptor protein , respectively . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Eps 8 binds , through its SH 3 domain , to either Abi 1 ( also called E3b1 ) or RN tre . ^^^ Abi 1 scaffolds together Eps 8 and Sos 1 , a dual specificity guanine nucleotide exchange factor for Ras and Rac proteins , thus facilitating the formation of a trimeric complex , in turn required for activation of Rac . ^^^ Furthermore , RN tre competes with Abi 1 for binding to Eps 8 , diverting the latter from its Rac activating function . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Phosphoinositide 3 kinase activates Rac by entering in a complex with Eps 8 , Abi 1 , and Sos 1 . ^^^ Here , we show that Abi 1 ( also known as E3b1 ) recruits PI3K , via p 85 , into a multimolecular signaling complex that includes Eps 8 and Sos 1 . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Sos 1 , a guanine nucleotide exchange factor ( GEF ) , eps 8 and Abi 1 , two signaling proteins , and the lipid kinase phosphoinositide 3 kinase ( PI 3 K ) , assemble in a multimolecular complex required for Rac activation leading to actin cytoskeletal remodeling . ^^^ Similarly to eps 8 , eps8Ls interact with Abi 1 and Sos 1 ; however , only eps8L1 and eps8L2 activate the Rac GEF activity of Sos 1 , and bind to actin in vivo . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| E3b1 , a binding partner of Eps 8 , plays a critical role in receptor tyrosine kinase ( RTK ) mediated Rac activation by facilitating the interaction of Eps 8 with Sos 1 and the consequent activation of the Rac specific guanine nucleotide exchange factor activity of Sos 1 . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Conversely , full length Eps 8 is auto inhibited in vitro , and interaction with the Abi 1 protein relieves this inhibition . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| E3b1 is a regulator of Rac because it can form a complex with Sos 1 and eps 8 , and such a Sos 1 e3B1 eps 8 complex serves as a guanine nucleotide exchange factor for Rac . ^^^ |
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| Interacting proteins: Q12929 and Q8IZP0 |
Pubmed |
SVM Score :0.0 |
| Regulation of actin cytoskeleton architecture by Eps 8 and Abi 1 . ^^^ The morphology of actin structures induced by Eps 8 was modulated by interactions with Abi 1 , which stimulated formation of actin cables in cultured cells and star like structures in Xenopus . ^^^ |
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