| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| The pro 4 / pro 5 motif of Shb binds in vitro particularly well to the Src , p 85 alpha PI 3 kinase and Eps 8 SH3 domains expressed as GST fusion proteins . ^^^ In vivo association between Shb SH 3 domain proteins 5 Src and Eps 8 was detected by coimmunoprecipitation . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps 8 and eps 15 , substrates lacking Src SH 2 homology domains . ^^^ Phosphorylation of two newly identified epidermal growth factor ( EGF ) receptor substrates , eps 8 and eps 15 , which do not possess Src homology ( SH 2 ) domains , was investigated using EGF receptor mutants of the autophosphorylation sites and deletion mutants of the carboxyl terminal region . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| The expression of the Shb binding proteins Eps 8 , Src , and p 85 PI3 kinase , PI 3 kinase activity , and MAP kinase activation were assessed in wild type NIH3T3 cells and in NIH3T3 cells overexpressing the Shb cDNA . ^^^ The Eps 8 protein content and Eps 8 mRNA steady state levels were downregulated , whereas the protein contents of Src and p 85 PI3 kinase were unaffected by Shb overexpression . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| Enhancement of tyrosyl phosphorylation and protein expression of eps 8 by 5 Src . ^^^ Analysis of eps 8 phosphotyrosine content in 5 Src transformed cells ( IV 5 ) revealed that both isoforms were highly tyrosyl phosphorylated and their readiness to be phosphorylated by Src in vitro further indicated that they were putative Src substrates as well . ^^^ Further in vitro binding assays demonstrated that p68eps8 was the major eps 8 isoforms that could be precipitated by bacterial fusion protein containing Src SH 3 . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| It possesses a Src homology 3 ( SH 3 ) motif , proline rich region , serine rich region and no catalytic domain , suggesting that it seems to be a signaling protein most similar to e3B1 , an eps 8 SH3 binding protein . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| Through its src homology 3 domain , Eps 8 interacts with RN tre . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| Further analysis demonstrated that , although TSA reduced the expression of Eps 8 in a dose and time dependent manner , both the protein expression and kinase activity of 5 Src remained constant , and the abundance and phosphotyrosine levels of Src substrates , including cortactin , focal adhesion kinase , p 130 ( Cas ) , paxillin , and Shc , were not altered . ^^^ When active Src expressing chicken embryonic cells were forced to overexpress p 97 ( Eps 8 ) , they became resistant to TSA mediated anti proliferation . ^^^ Thus , our results highlight a critical role for p 97 ( Eps 8 ) in TSA exerted growth inhibition of 5 Src transformed cells . . ^^^ |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q12929 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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