| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Direct binding of eps 8 to the juxtamembrane domain of EGFR is phosphotyrosine and SH 2 independent . ^^^ Here we report that eps 8 binds directly to the juxtamembrane region of EGFR through a domain that does not bear resemblance to SH 2 domains and by a mechanism that does not require the presence of phosphotyrosine residues . ^^^ Thus , the physical association between EGFR and eps 8 represents a novel interaction between RTKs and their substrates . . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps 8 and eps 15 , substrates lacking Src SH 2 homology domains . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Constitutive phosphorylation of eps 8 in tumor cell lines : relevance to malignant transformation . eps 8 , a recently identified tyrosine kinase substrate , has been shown to augment epidermal growth factor ( EGF ) responsiveness , implicating it in EGF receptor ( EGFR ) mediated mitogenic signaling . ^^^ In NIH 3T3 cells overexpressing EGFR ( NIH EGFR ) , eps 8 becomes rapidly phosphorylated upon EGF stimulation . ^^^ In human tumor cell lines , we detected constitutive tyrosine phosphorylation of eps 8 , with a stoichiometry ( approximately 5 % ) similar to that associated with potent mitogenic response in NIH EGFR cells . ^^^ Overexpression of eps 8 was able to transform NIH 3T3 cells under limiting conditions of activation of the EGFR pathway . ^^^ Our results indicate that eps 8 is physiologically part of the EGFR activated signaling and that its alterations can contribute to the malignant phenotype . . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| By applying this technique to the study of the epidermal growth factor receptor ( EGFR ) signaling pathway , we have isolated a cDNA , designated eps 8 , which predicts a approximately 92 kDa protein containing an SH 3 domain . ^^^ The product of the eps 8 gene is tyrosine phosphorylated in vivo following EGF stimulation of intact cells and associates with the EGFR , despite the lack of a functional SH 2 domain . ^^^ Adoptive expression of the eps 8 cDNA in fibroblastic or hematopoietic target cells expressing the EGFR resulted in increased mitogenic response to EGF , implicating the eps 8 gene product in the control of mitogenic signals . . ^^^ Eps 8 , a substrate for the epidermal growth factor receptor kinase , enhances EGF dependent mitogenic signals . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| The recently identified Eps 8 protein , which contains an SH 3 domain , is coupled functionally and physically to the EGFR and is tyrosine phosphorylated by this receptor and other receptors as well . ^^^ Conversely , expression of Eps 8 , but not of other EGFR substrates such as Shc or Eps 15 , is virtually extinguished in non proliferating , terminally differentiated murine myogenic cells . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| During the screening , we found that EPS 8 , which is a substrate for activated EGF receptor ( EGFR ) , specifically interacted with Dvl 1 . ^^^ Dvl 1 was hyperphosphorylated in the presence of EPS 8 , whereas the tyrosine phosphorylation of EPS 8 by activated EGFR was inhibited in the presence of Dvl 1 . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Eps 8 is a substrate of the EGFR , which is held in a complex with Sos 1 by the adaptor protein E3bl ( ref . 2 ) , thereby mediating activation of Rac . ^^^ By entering in a complex with Eps 8 , RN tre acts on Rab 5 and inhibits internalization of the EGFR . ^^^ Thus , depending on its state of association with E3b1 or RN tre , Eps 8 participates in both EGFR signalling through Rac , and trafficking through Rab5 . . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Some molecules , such as Grb 2 , were primarily found associated with surface EGFR , whereas others , such as Eps 8 , were found only with intracellular receptors . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Eps 8 , originally identified as a substrate for the kinase activity of the epidermal growth factor receptor ( EGFR ) , displays a domain organization typical of a signaling molecule that includes a putative N terminal PTB domain , a central SH 3 domain , and a C terminal `` effector region ' ' . ^^^ On the other hand , RN tre , a Rab 5 GTPase activating protein , by entering in a complex with Eps 8 , inhibits EGFR internalization . ^^^ Thus , depending on its engagement in different complexes , Eps 8 participates to EGFR signaling through Rac and endocytosis through Rab5 . . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| Epidermal growth factor receptor pathway substrate 8 ( Eps 8 ) expression in maturing testis . ^^^ AIM : Although epidermal growth factor receptors are expressed in the testes , whether they signal through epidermal growth factor receptor pathway substrate 8 ( Eps 8 ) is unknown . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| We have identified EGFR substrate protein 8 ( EPS 8 ) as a novel component of the EGFR localization complex that links receptor trafficking to cell fate specification . ^^^ EPS 8 expression is upregulated in the primary VPCs , where it creates a positive feedback loop in the EGFR / RAS / MAPK pathway . ^^^ The membrane associated guanylate kinase LIN 2 recruits EPS 8 into the receptor localization complex to retain the EGFR on the basolateral plasma membrane , and thus allow maximal receptor activation in the primary cell lineage . ^^^ Low levels of EPS 8 in the neighboring secondary VPCs result in the rapid degradation of the EGFR , allowing these cells to adopt the secondary cell fate . ^^^ |
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| Interacting proteins: Q12929 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
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