| Interacting proteins: Q6ZWJ1 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Synip : a novel insulin regulated syntaxin 4 binding protein mediating GLUT 4 translocation in adipocytes . ^^^ We have isolated a novel syntaxin 4 binding protein , Synip , which specifically interacts with syntaxin 4 . ^^^ Insulin induces a dissociation of the Synip : syntaxin 4 complex due to an apparent decrease in the binding affinity of Synip for syntaxin 4 . ^^^ In contrast , the carboxyterminal domain of Synip does not dissociate from syntaxin 4 in response to insulin stimulation but inhibits glucose transport and GLUT 4 translocation . ^^^ These data implicate Synip as an insulin regulated syntaxin 4 binding protein directly involved in the control of glucose transport and GLUT 4 vesicle translocation . . ^^^ |
|
| Interacting proteins: Q6ZWJ1 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 4 and Synip ( syntaxin 4 interacting protein ) regulate insulin secretion in the pancreatic beta HC 9 cell . ^^^ Furthermore , these cells express the selective syntaxin 4 binding protein Synip ( syntaxin 4 interacting protein ) , and Synip was specifically co immunoprecipitated with syntaxin 4 but not syntaxin 1 . ^^^ Overexpression of the full length Synip protein ( Synip / wild type ) inhibited VAMP 2 association with syntaxin 4 and decreased glucose stimulated insulin secretion . ^^^ This did not occur with a Synip mutant ( Synip / DeltaEF ) that was incapable of binding syntaxin 4 . ^^^ These data demonstrate that syntaxin 4 plays a functional role on insulin release and granule fusion in beta cells and that this process is regulated by the syntaxin 4 specific binding protein Synip . . ^^^ |
|
| Interacting proteins: Q6ZWJ1 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Although wild type Synip ( WT Synip ) undergoes an insulin stimulated dissociation from Syntaxin 4 , the Synip serine 99 to phenylalanine mutant ( S99F Synip ) is resistant to Akt 2 phosphorylation and fails to display insulin stimulated Syntaxin 4 dissociation . ^^^ These data demonstrate that insulin activation of Akt 2 specifically regulates the docking / fusion step of GLUT 4 containing vesicles at the plasma membrane through the regulation of Synip phosphorylation and Synip Syntaxin 4 interaction . . ^^^ |
|
| Interacting proteins: Q6ZWJ1 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Another potential connecting substrate is the protein Synip , which associates with the SNARE syntaxin 4 . ^^^ A recent study presents evidence that Akt phosphorylates Synip on serine 99 , at least in vitro , and proposes that this phosphorylation enables GLUT 4 translocation by causing the dissociation of Synip from syntaxin 4 . ^^^ |
|
| Interacting proteins: Q6ZWJ1 and Q12846 |
Pubmed |
SVM Score :0.0 |
| At the cell periphery , GLUT 4 containing vesicles tether , dock , and fuse with the PM assisted by the exocyst complex followed by engagement of a soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) complex [ with vesicle associated membrane protein ( VAMP ) 2 as the vesicular ( 5 ) SNARE and soluble NSF attachment protein ( SNAP ) 23 and syntaxin 4 as target ( t ) SNAREs ] regulated by the accessory proteins Munc18c , Synip and Tomosyn . ^^^ |
|