| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.55915709 |
| VAMP 2 and VAMP3 / cellubrevin ( 5 SNARE ' s ) have been shown to interact with the t SNARE ' s syntaxin 4 and SNAP 23 . 0.55915709^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.52992015 |
| Activation of neutrophils led to the interaction of VAMP 2 with the plasma membrane Q SNARE syntaxin 4 , and anti syntaxin 4 Abs inhibited exocytosis of specific and tertiary granules in electropermeabilized neutrophils . 0.52992015^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.53820986 |
| Using a yeast two hybrid assay we demonstrate that the VAMP 2 like domain in Tomosyn facilitates the interaction with Syntaxin 4 . 0.53820986^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| In contrast , syntaxin 4 , VAMP 2 , and the 39 kDa isoform of secretory carrier membrane protein ( SCAMP ) were present . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The demonstrated localization of syntaxin 4 in the apical plasma membrane of collecting duct principal cells , coupled with previous demonstration of syntaxin 4 ' s putative cognate receptor VAMP 2 in aquaporin 2 containing vesicles , supports the view that these proteins could play a role of aquaporin 2 vesicle targeting to the apical plasma membrane . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization using isoform specific oligonucleotide probes to different exocytotic protein messenger RNAs ( mRNAs ) showed strong hybridization signal for synaptotagmin 1 , cysteine string protein ( CSP ) , VAMP 2 ( vesicle associated membrane protein ) , cellubrevin , munc 18 ( mammalian homologue of unc 18 ) , SNAP 25a ( synaptosomal associated protein of 25 kDa ) , syntaxin 1A , syntaxin 4 , syntaxin 5 , and alpha SNAP ( soluble NSF attachment protein ) in the anterior and intermediate , but not in the posterior lobe of the pituitary . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Recently , several SNARE proteins were found in adipocytes : vesicle associated membrane protein ( VAMP 2 ) , its related homologue cellubrevin , and syntaxin 4 . ^^^ Furthermore , treatment of the permeabilized adipocytes with glutathione S transferase fusion proteins encoding soluble forms of VAMP 2 or syntaxin 4 also effectively blocked insulin regulated GLUT 4 translocation . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The specific interaction between Munc 18c and syntaxin 4 is of interest because aside from syntaxin 1A , which is not expressed in adipocytes , syntaxin 4 is the only syntaxin that binds to VAMP 2 . ^^^ Using a three way binding assay , it was shown that Munc 18c inhibits the binding of syntaxin 4 to VAMP 2 . ^^^ These data suggest that syntaxin 4 / Munc 18c / VAMP 2 may play a role in the docking / fusion of intracellular GLUT 4 containing vesicles with the cell surface in adipocytes . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 4 , VAMP 2 , and / or VAMP3 / cellubrevin are functional target membrane and vesicle SNAP receptors for insulin stimulated GLUT 4 translocation in adipocytes . ^^^ Together , these data demonstrate that syntaxin 4 , VAMP 2 , and / or VAMP3 / cellubrevin can function as target membrane and vesicle SNAP receptors , respectively , for insulin responsive GLUT 4 translocation to the plasma membrane . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The plasma membranes of insulin responsive cells have also been shown to contain syntaxin 4 and the 25 kDa synaptosome associated protein ( SNAP 25 ) , two proteins that form a complex with VAMP 2 . ^^^ The potential functional involvement of VAMP 2 , SNAP 25 and syntaxin 4 in the trafficking of GLUT 4 was assessed in the present study by determining the effect on GLUT 4 translocation of microinjection of toxins that specifically cleave VAMPs or SNAP 25 , or microinjection of specific peptides from VAMP 2 and syntaxin 4 . ^^^ These data taken together strongly suggest a role for VAMP 2 in GLUT 4 trafficking and also for syntaxin 4 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| By using surface plasmon resonance we show that VAMP 2 , Syntaxin 4 , and Syndet form a ternary SDS resistant SNARE complex . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Binary interactions of the SNARE proteins syntaxin 4 , SNAP 23 , and VAMP 2 and their regulation by phosphorylation . ^^^ In this study , we defined the binding characteristics of three SNARE proteins : SNAP 23 , VAMP 2 , and syntaxin 4 . ^^^ Binary , saturable interactions among all three partners ( VAMP 2 syntaxin 4 , VAMP 2 SNAP 23 , and SNAP 23 syntaxin 4 ) were measured in vitro . ^^^ Unlike its neuronal counterpart , SNAP 23 did not potentiate VAMP 2 binding to its putative t SNARE partner , syntaxin 4 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulates glucose transporter ( GLUT ) 4 vesicle translocation from intracellular storage sites to the plasma membrane in 3T3L1 adipocytes through a VAMP 2 and syntaxin 4 dependent mechanism . ^^^ We have observed that Munc18c , a mammalian homolog of the yeast syntaxin binding protein n Sec1p , competed for the binding of VAMP 2 to syntaxin 4 . ^^^ Together , these data suggest that Munc18c inhibits the docking / fusion of GLUT 4 containing vesicles by blocking the binding of VAMP 2 to syntaxin 4 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNARE complex proteins , including the cognate pair VAMP 2 and syntaxin 4 , are expressed in cultured oligodendrocytes . ^^^ During differentiation , expression of the vesicular SNARE VAMP 2 , the small GTP binding protein Rab3a , and the target SNARE syntaxin 4 were up regulated . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| A measurable fraction of SNAP 23 was sedimented with cytoskeletal elements when extracted with Triton 10 100 , unlike VAMP 2 and syntaxin 4 , which were exclusively soluble in detergent . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Vesicle shuffling to the plasma membrane involves proteins such as vesicle associated membrane protein VAMP 2 , syntaxin 4 and the small GTPase Rab3a . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulated glucose transport and GLUT 4 translocation require regulated interactions between the 5 SNARE , VAMP 2 , and the t SNARE , syntaxin 4 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Mediation of complex formation between syntaxin 4 and VAMP 2 . ^^^ Both syntaxin 4 and VAMP 2 are implicated in insulin regulation of glucose transporter 4 ( GLUT 4 ) trafficking in adipocytes as target ( t ) soluble N ethylmaleimide sensitive factor attachment protein receptors ( SNARE ) and vesicle ( 5 ) SNARE proteins , respectively , which mediate fusion of GLUT 4 containing vesicles with the plasma membrane . ^^^ Wild type SNAP 23 ( SNAP 23 WT ) promoted the interaction between syntaxin 4 and VAMP 2 both in vitro and in vivo . ^^^ Although SNAP 23 DeltaC49 bound to neither syntaxin 4 nor VAMP 2 , the SNAP 23 DeltaC8 mutant bound to syntaxin 4 but not to VAMP 2 . ^^^ In addition , although SNAP 23 DeltaC8 bound to syntaxin 4 , it did not mediate the interaction between syntaxin 4 and VAMP 2 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| However , when vesicle associated membrane protein ( VAMP ) 2 was immunoprecipitated from lysates of parotid acinar cells , syntaxin 4 and SNAP 23 were not coprecipitated with VAMP 2 , although syntaxin 1 and SNAP 25 , t SNAREs in neuronal cells , were clearly coprecipitated with VAMP 2 from brain lysates . ^^^ Inversely , when syntaxin 4 was immunoprecipitated from parotid lysates , SNAP 23 , Munc18c , and N ethylmaleimide sensitive fusion protein ( NSF ) were coprecipitated , but VAMP 2 was again undetectable . ^^^ These results suggest that the interaction between syntaxin 4 , SNAP 23 and VAMP 2 is fairly weak and their concentrations in the cell lysate are insufficient to make a readily detectable complex , and that bindings between these proteins are hindered by other proteins in parotid acinar cells . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Studies have identified key roles for vesicle associated membrane protein 2 ( VAMP 2 ) and syntaxin 4 in this event , and more recently have also implicated SNAP 23 and Munc18c in this process . ^^^ Such analyses suggest that in 3T3 L 1 adipocytes there are approximately 374 , 000 copies of syntaxin 4 , 1 . 15 10 10 ( 6 ) copies of SNAP 23 , 495 , 000 copies of VAMP 2 , 4 . 3 10 10 ( 6 ) copies of cellubrevin and 452 , 000 copies of Munc18c per cell , compared to previous estimates of 280 , 000 copies of Glut 4 . ^^^ Thus , the main SNARE proteins involved in insulin stimulated Glut 4 exocytosis ( syntaxin 4 and VAMP 2 ) are expressed in approximately equimolar amounts in adipocytes , whereas by contrast the endosomal 5 SNARE cellubrevin is present at approximately 10 fold higher levels and the t SNARE SNAP 23 is also present in an approximately 3 fold molar excess . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Studies have suggested an important role for synaptobrevin and syntaxin homologues in this event , particularly the 5 soluble N ethylmaleimide attachment protein receptors ( SNAREs ) cellubrevin and vesicle associated membrane protein 2 ( VAMP 2 ) and the t SNARE syntaxin 4 , but the expression of these proteins has not been studied in insulin resistant tissues . ^^^ In contrast , cellubrevin , VAMP 2 , and syntaxin 4 protein levels were elevated ( 2 . 8 fold , P = 0 . 02 ; 3 . 7 fold , P = 0 . 01 ; and 2 . 2 fold , P < 0 . 05 , respectively ) in skeletal muscle from ZDF rats compared with lean controls . ^^^ Restoration of normoglycemia and normoinsulinemia in ZDF rats with rosiglitazone ( 30 micromol / kg ) normalized cellubrevin , VAMP 2 , and syntaxin 4 protein to levels approaching those observed in lean control animals . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Identification and cloning of the SNARE proteins VAMP 2 and syntaxin 4 from HL 60 cells and human neutrophils . ^^^ As with VAMP 2 , expression of syntaxin 4 ( determined by Northern blots ) also increased , but by only 50 % , during differentiation of HL 60 cells . ^^^ These studies demonstrate that neutrophils and their progenitors possess mRNA for the fusion proteins VAMP 2 and syntaxin 4 , and that their transcription increases during differentiation , concurrent with the functional maturation of myeloid cells . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The SNARE isoforms VAMP 2 , SNAP 23 , and syntaxin 4 have been described in secretory processes in myeloid cells . ^^^ OBJECTIVE : We sought to determine whether eosinophils express the t SNARE isoforms SNAP 23 and syntaxin 4 as potential binding targets for VAMP 2 during exocytosis . ^^^ CONCLUSIONS : The plasma membrane associated t SNAREs SNAP 23 and syntaxin 4 are expressed in human eosinophils and are likely candidates for association with VAMP 2 during docking , which is followed by exocytosis . ^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Glut 4 vesicle fusion is thought to be catalyzed by the interaction of the vesicle soluble N ethyl maleimide sensitive fusion protein attachment protein receptor VAMP 2 with the target soluble N ethyl maleimide sensitive fusion protein attachment protein receptors SNAP 23 and syntaxin 4 . ^^^ Insulin stimulation had no effect on the plasma membrane raft association of SNAP 23 or syntaxin 4 but promoted VAMP 2 insertion into plasma membrane rafts . ^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| VAMP 2 , syntaxin 4 , and SNAP 23 are plausible candidate SNARE proteins for non neuronal exocytosis . ^^^ The protein protein interaction between syntaxin 4 and VAMP 2 with their transmembrane domains was markedly inhibited on coexpression of munc18c . ^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| This occurred concomitantly with a block in insulin stimulated association of syntaxin 4 with its cognate GLUT 4 vesicle receptor protein ( 5 SNARE ) , VAMP 2 . ^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| This fusion event is mediated by SNARE proteins , syntaxin 4 , SNAP 23 and VAMP 2 , through a process regulated by accessory proteins whose roles are still unclear . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNAP 23 interacted with syntaxin 3 , syntaxin 4 , VAMP 2 , VAMP 3 and VAMP 8 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the full length Synip protein ( Synip / wild type ) inhibited VAMP 2 association with syntaxin 4 and decreased glucose stimulated insulin secretion . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNAREs were found either excluded ( syntaxin 2 ) , equally distributed between raft and non raft fractions ( syntaxin 4 , VAMP 8 , VAMP 2 ) , or selectively enriched in rafts ( syntaxin 3 , SNAP 23 ) . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Several `` soluble N ethylmaleimide sensitive fusion protein attachment protein receptor ' ' ( SNARE ) proteins have been identified in rat parotid acinar cells , including VAMP 2 , syntaxin 4 , and SNAP 23 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| They also express components of the insulin sensitive vesicular transport machinery , namely , VAMP 2 , syntaxin 4 , and IRAP , the last of these being the other marker of insulin regulated vesicular traffic along with Glut 4 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We demonstrated expression of plasma membrane proteins ( syntaxin 4 and syntaxin 6 ) and specific / gelatinase granule membrane proteins ( SNAP 25 and VAMP 2 ) in the peripheral blood neutrophils of patients with chronic myeloid leukemia . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The fusion of Glut 4 containing vesicles at the plasma membrane is known to involve the target SNAREs syntaxin 4 and SNAP 23 and the vesicle SNARE VAMP 2 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Although triggering exocytosis did not alter the absolute amount of SNAP 23 bound to SNAREs , after stimulation essentially all of the SNAP 23 bound to the plasma membrane SNARE syntaxin 4 and the vesicle SNARE VAMP 2 was phosphorylated . ^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Fusion of the GLUT 4 containing vesicles with the plasma membrane is mediated by a soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) complex consisting of vesicle associated membrane protein 2 ( VAMP 2 ) , 23 kDa synaptosomal associated protein ( SNAP 23 ) , and syntaxin 4 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| These cells did not express syntaxin 1A , vesicle associated membrane protein 1 ( VAMP 1 ) , or synaptosomal associated protein of 25 kDa ( SNAP 25 ) , but did express a family of SNARE proteins that has been implicated in glucose transporter trafficking , including syntaxin 4 , vesicle associated membrane protein 2 ( VAMP 2 ) , and synaptosomal associated protein of 23 kDa ( SNAP 23 ) . cDNAs encoding variants of SNAP 23 were co transfected with Myc tagged EAAC 1 to determine if SNAP 23 function was required for maintenance of EAAC 1 surface expression . ^^^ |
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| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Further analyses demonstrate that cytLEK 1 and SNAP 25 colocalize and coprecipitate with Rab11a , myosin Vb , VAMP 2 , and syntaxin 4 , components of the plasma membrane recycling pathway . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| These data indicate that at least two SNARE complexes , made up of syntaxin 4 / SNAP 23 / VAMP 1 and syntaxin 4 / SNAP 23 / VAMP 2 , are involved in the exocytosis of specific and tertiary granules , whereas interactions between syntaxin 4 and VAMP 1 / VAMP 7 are involved in the exocytosis of azurophilic granules . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Insulin stimulated glucose uptake requires the fusion of GLUT 4 transporter containing vesicles with the plasma membrane , a process that depends on the SNARE ( soluble N ethylmaleimide sensitive fusion factor attachment receptor ) proteins VAMP 2 ( vesicle associated membrane protein 2 ) and syntaxin 4 ( Stx 4 ) / SNAP23 ( soluble N ethylmaleimide sensitive fusion factor attachment protein 23 ) . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Coupled to the recent detection of vesicle associated membrane protein 2 and cellubrevin in skeletal muscle , syntaxin 4 may play a role in membrane traffic in this tissue . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we have confirmed the presence of syntaxin 4 in fat and of synaptobrevin 2 in skeletal muscle by PCR amplification and Southern hybridization analysis . ^^^ Using the yeast two hybrid system , an interaction was observed between the full length cytoplasmic domains of syntaxin 4 and synaptobrevin 2 , a vesicle membrane SNARE previously shown by others to be associated with vesicles carrying the GLUT 4 glucose transporter protein , but no interaction was seen with synaptobrevin 1 . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The vesicle targeting proteins synaptobrevin 2 and syntaxin 4 are proposed to play roles in this process . . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| An interaction of SNAP 23 and syntaxin 4 on the plasma membrane with vesicle associated synaptobrevin 2 and / or cellubrevin , known as SNAP ( soluble N ethyl maleimide sensitive factor attachment protein ) receptors or SNAREs , has been proposed to provide the targeting and / or fusion apparatus for insulin stimulated translocation of the GLUT 4 isoform of glucose transporter to the plasma membrane . ^^^ |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63027 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|