| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.83902235 |
| The biochemical consequences of SNARE protein phosphorylation included a reduced interaction between SNAP 25 and phosphorylated syntaxin 4 and an enhanced interaction between phosphorylated syntaxin 1A and the synaptic vesicle protein synaptotagmin 1 , a potential Ca2+ sensor in triggering synaptic vesicle exocytosis . 0.83902235^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.52099544 |
| These results suggest that the interaction between syntaxin 4 , SNAP 23 and VAMP 2 is fairly weak and their concentrations in the cell lysate are insufficient to make a readily detectable complex , and that bindings between these proteins are hindered by other proteins in parotid acinar cells . . 0.52099544^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Insulin responsive tissues contain the core complex protein SNAP 25 ( synaptosomal associated protein 25 ) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2 . ^^^ It describes the isolation and characterization of cDNA molecules encoding SNAP 25 A and B isoforms , syntaxin 4 and synaptobrevins ( also known as vehicle associated membrane proteins ) from two major human insulin responsive tissues , skeletal muscle and fat . ^^^ Flow cytometry of low density microsomes isolated from fat cells was used to demonstrate the binding of syntaxin 4 to a subset of vesicles carrying GLUT 4 protein ; whereas SNAP 25 on its own bound poorly to these vesicles , the syntaxin 4 SNAP 25 complex gave a strong interaction . . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 4 , SNAP 23 and / or SNAP 25 are also implicated in this process . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| By using a combination of immunoblotting , detergent extraction , and anion exchange chromatography , we identified p 35 as Syntaxin 4 and p 25 as the recently identified murine SNAP 25 homologue , Syndet ( mSNAP 23 ) . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We found that platelet membranes contain a full complement of interacting proteins VAMP , SNAP 25 , and syntaxin 4 that are necessary for vesicle docking and fusion with the plasma membrane . ^^^ This platelet Sec 1 protein ( PSP ) bound to syntaxin 4 and thereby excluded the binding of SNAP 25 with syntaxin 4 , an interaction critical to vesicle docking . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Synaptosome associated 23 kDa protein ( SNAP 23 ) is a widely expressed isoform of SNAP 25 , the principal t SNARE of neuronal cells , and colocalizes with syntaxin 4 in the plasma membrane of 3T3 L 1 adipocytes . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| A combination of reverse transcription polymerase chain reaction ( RT PCR ) and northern and western blotting is used to show expression of the target membrane SNARE ( soluble NSF attachment protein receptor ) , proteins SNAP 25 and syntaxin 4 and the vesicular SNARE protein VAMP ( synaptobrevin ) , the minimum molecular requirements for core exocytotic complex formation . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We demonstrated expression of plasma membrane proteins ( syntaxin 4 and syntaxin 6 ) and specific / gelatinase granule membrane proteins ( SNAP 25 and VAMP 2 ) in the peripheral blood neutrophils of patients with chronic myeloid leukemia . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| These cells did not express syntaxin 1A , vesicle associated membrane protein 1 ( VAMP 1 ) , or synaptosomal associated protein of 25 kDa ( SNAP 25 ) , but did express a family of SNARE proteins that has been implicated in glucose transporter trafficking , including syntaxin 4 , vesicle associated membrane protein 2 ( VAMP 2 ) , and synaptosomal associated protein of 23 kDa ( SNAP 23 ) . cDNAs encoding variants of SNAP 23 were co transfected with Myc tagged EAAC 1 to determine if SNAP 23 function was required for maintenance of EAAC 1 surface expression . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Further analyses demonstrate that cytLEK 1 and SNAP 25 colocalize and coprecipitate with Rab11a , myosin Vb , VAMP 2 , and syntaxin 4 , components of the plasma membrane recycling pathway . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The plasma membranes of insulin responsive cells have also been shown to contain syntaxin 4 and the 25 kDa synaptosome associated protein ( SNAP 25 ) , two proteins that form a complex with VAMP 2 . ^^^ The potential functional involvement of VAMP 2 , SNAP 25 and syntaxin 4 in the trafficking of GLUT 4 was assessed in the present study by determining the effect on GLUT 4 translocation of microinjection of toxins that specifically cleave VAMPs or SNAP 25 , or microinjection of specific peptides from VAMP 2 and syntaxin 4 . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 4 , VAMP 2 , and / or VAMP3 / cellubrevin are functional target membrane and vesicle SNAP receptors for insulin stimulated GLUT 4 translocation in adipocytes . ^^^ Together , these data demonstrate that syntaxin 4 , VAMP 2 , and / or VAMP3 / cellubrevin can function as target membrane and vesicle SNAP receptors , respectively , for insulin responsive GLUT 4 translocation to the plasma membrane . . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Roles of vesicle associated membrane protein , SNAP 23 , and syntaxin 4 . ^^^ To understand the molecular basis of granule release from platelets , we examined the role of vesicle associated membrane protein , SNAP 23 , and syntaxin 4 in alpha granule secretion . ^^^ A vesicle associated membrane protein , SNAP 23 , and syntaxin 4 were detected in platelet lysate . ^^^ These results show that vesicle associated membrane protein , SNAP 23 , and syntaxin 4 form a heterotrimeric complex in platelets that disassembles with activation and demonstrate that alpha granule release is dependent on vesicle SNAP receptor target SNAP receptor ( vSNARE tSNARE ) interactions . . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation of syntaxin 4 and SNAP 23 shows association of these two proteins in rat adipose cell plasma membranes , and insulin stimulation does not alter the SNAP 23 / syntaxin 4 complex . ^^^ In addition , we demonstrate for the first time the participation of SNAP 23 , along with syntaxin 4 and VAMP2 / 3 , in the formation of 20S SNARE complexes prepared using rat adipose cell membranes and recombinant alpha SNAP and NSF proteins . ^^^ These data demonstrate that rat SNAP 23 associates with syntaxin 4 before insulin stimulation and is present in the SNARE complexes known to mediate the translocation of GLUT 4 from intracellular vesicles to the plasma membrane of rat adipose cells . . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| An interaction of SNAP 23 and syntaxin 4 on the plasma membrane with vesicle associated synaptobrevin 2 and / or cellubrevin , known as SNAP ( soluble N ethyl maleimide sensitive factor attachment protein ) receptors or SNAREs , has been proposed to provide the targeting and / or fusion apparatus for insulin stimulated translocation of the GLUT 4 isoform of glucose transporter to the plasma membrane . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The SNARE isoforms VAMP 2 , SNAP 23 , and syntaxin 4 have been described in secretory processes in myeloid cells . ^^^ OBJECTIVE : We sought to determine whether eosinophils express the t SNARE isoforms SNAP 23 and syntaxin 4 as potential binding targets for VAMP 2 during exocytosis . ^^^ METHODS : Human peripheral blood eosinophils ( > 97 % ) from atopic subjects were subjected to RT PCR and sequence analysis by using specific primers for SNAP 23 and syntaxin 4 . ^^^ RESULTS : Nucleotide sequences obtained from PCR products exhibited nearly identical ( > 95 % ) homology with reported sequences for human SNAP 23 and syntaxin 4 . ^^^ Both SNAP 23 and syntaxin 4 were present in plasma membranes , with some staining in endoplasmic reticulum and Golgi membranes . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| At the cell periphery , GLUT 4 containing vesicles tether , dock , and fuse with the PM assisted by the exocyst complex followed by engagement of a soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) complex [ with vesicle associated membrane protein ( VAMP ) 2 as the vesicular ( 5 ) SNARE and soluble NSF attachment protein ( SNAP ) 23 and syntaxin 4 as target ( t ) SNAREs ] regulated by the accessory proteins Munc18c , Synip and Tomosyn . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 2 , Syntaxin 3 , Syntaxin 4 , vesicle associated membrane protein ( VAMP ) 2 , VAMP 3 , and synaptosome associated protein ( SNAP ) 23 could be readily detected in normal human PC obtained from intestinal lamina propria and blood , as well as in human PC lines . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Previous studies indicated that the 5 SNARE , vesicle associated membrane protein ( VAMP ) 2 , as well as t SNAREs ( SNAP 23 , syntaxin 4 and 6 ) are implicated in exocytosis from human granulocytes . ^^^ Syntaxin 4 , but not syntaxin 6 , was found to interact with SNAP 23 and was partially involved in mediator secretion from multiple compartments . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We also review recent findings showing that the candidate exocytotic SNAREs SNAP 23 and syntaxin 4 colocalize with SCAMPs , especially SCAMP 2 , and that SCAMP 2 is likely to be a partner in the compound exocytotic process . . ^^^ |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P60880 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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