| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Solution structure of the HMG box domain in the SSRP 1 subunit of FACT . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| This approach resulted in the isolation of FACT , a heterodimer in humans comprised of Spt 16 and SSRP 1 . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Here we show that FACT comprises a new human homologue of the Saccharomyces cerevisiae Spt16 / Cdc68 protein and the high mobility group 1 like protein structure specific recognition protein 1 . ^^^ Consistent with our biochemical analysis of FACT , we provide evidence that Spt16 / Cdc68 is involved in transcript elongation in vivo . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| This kinase complex contains casein kinase 2 ( CK 2 ) and the chromatin transcriptional elongation factor FACT ( a heterodimer of hSpt 16 and SSRP 1 ) . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| The FACT complex of vertebrate cells , comprising the Cdc 68 ( Spt 16 ) and SSRP 1 proteins , facilitates transcription elongation on a nucleosomal template and modulates the elongation inhibitory effects of the DSIF complex in vitro . ^^^ The CP components Cdc 68 and Pob 3 closely resemble the FACT components , except that the C terminal high mobility group ( HMG ) box domain of SSRP 1 is not found in the yeast homolog Pob 3 . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Similar homologies were found with the other members of the Cdc 68 homologue family of transcriptional activators , including the human FACT protein . . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Spt16 / Cdc68 , Pob 3 , and Nhp 6 collaborate in vitro and in vivo as the yeast factor SPN , which is homologous to human FACT . ^^^ |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| The larger of the two subunits of FACT is Spt16 / Cdc68 , while the smaller is termed SSRP 1 ( vertebrates ) or Pob 3 ( budding yeast ) . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| The FACT ( facilitates chromatin transcription ) complex , a heterodimer of hSpt 16 and SSRP 1 , is a chromatin structure modulator whose involvement in transcription and DNA replication has been reported . ^^^ Here we show that nucleosome binding activity of FACT complex is regulated by poly ( ADP ribosyl ) ation . hSpt 16 , the large subunit of FACT , is poly ( ADP ribosyl ) ated by poly ( ADP ribose ) polymerase 1 ( PARP 1 ) resulting from physical interaction between these two proteins . ^^^ The enhanced hSpt 16 poly ( ADP ribosyl ) ation level correlates with the dissociation of FACT from chromatin in response to DNA damage . ^^^ Our findings suggest that poly ( ADP ribosyl ) ation of hSpt 16 by PARP 1 play regulatory roles for FACT mediated chromatin remodeling . . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| The published consensus steroid binding sequence previously identified in cytochromes is in fact 80 amino acids upstream from its previously defined position . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Previously , we purified a UV responsive p 53 serine 392 kinase from F 9 and HeLa cells and found that its activity is attributed to a high molecular weight protein complex containing the protein kinase CK 2 , along with the chromatin associated factors hSPT 16 and SSRP 1 . ^^^ Here we determine that these proteins interact in vitro and in cells via non overlapping domains and provide evidence consistent with the idea that hSPT 16 and SSRP 1 change the conformation of CK 2 upon binding such that it specifically targets p 53 over other substrates . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Earlier studies of yeast FACT , which indicated the apparent dispensability of conserved sequences at the N terminus of the FACT subunit Cdc68 / Spt16 , prompted genetic and biochemical studies reported here that suggest the domain organization for Spt 16 and the other FACT subunit Pob 3 , the yeast homolog of the metazoan SSRP 1 protein . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Using co immunoprecipitation we have identified in Arabidopsis thaliana cells the FAcilitates Chromatin Transcription ( FACT ) complex , consisting of the 120 kDa Spt 16 and the 71 kDa SSRP 1 proteins . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of SSRP 1 by CK 2 inhibited the nonspecific DNA binding activity of SSRP 1 and FACT ( facilitating chromatin mediated transcription ) complex in vitro . ^^^ |
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| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q08945 and Q9Y5B9 |
Pubmed |
SVM Score :0.0 |
| NA |
|