| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| Studies on LAT , SLP 76 , Itk and Vav have revealed their role in the activation of Ras and phospholipase Cgamma 1 Ca2+ signalling pathways . ^^^ |
|
| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| Itk / Emt / Tsk activation in response to CD 3 cross linking in Jurkat T cells requires ZAP 70 and Lat and is independent of membrane recruitment . ^^^ Lat , a prominent in vivo substrate of ZAP 70 that mediates assembly of multimolecular signaling complexes at the plasma membrane of T cells was also found to be required for TCR stimulated Itk activation . ^^^ Itk could not be activated by CD 3 cross linking in a Lat negative cell line , unless Lat expression was restored . ^^^ Lat and Itk were observed to co associate in response to CD 3 cross linking in Jurkat T cells , but not in P 116 T cells . ^^^ The Lat Itk association correlated with Lat tyrosine phosphorylation , which was deficient in the P 116 T cells . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| First , the inositide binding pocket of the Itk pleckstrin homology domain directs the constitutive association of Itk with buoyant membranes that are the primary site of TCR activation and are enriched in both Lck and LAT . ^^^ Second , the Itk proline rich region binds to Grb 2 and LAT . ^^^ Together , these observations suggest that multivalent interactions recruit Itk to LAT nucleated signaling complexes and facilitate the activation of LAT associated phospholipase Cgamma 1 by Itk . . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| However , phospholipase Cgamma 1 ( PLCgamma 1 ) and LAT were strikingly hypophosphorylated in CD 148 expressing cells following TCR stimulation , whereas the phosphorylation levels of Slp 76 and Itk were modestly reduced . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| In this report , we show by generation of LAT truncation mutants that the Syk family kinase ZAP 70 and the Tec family kinase Itk favor phosphorylation of carboxy terminal tyrosines in LAT . ^^^ Further , overexpression of a kinase deficient mutant of Itk in T cells reduced both the tyrosine phosphorylation of endogenous LAT and the recruitment of Vav to LAT complexes . ^^^ These data indicate that kinases from distinct PTK families are likely responsible for LAT phosphorylation following T cell activation and that Itk kinase activity promotes recruitment of Vav to LAT . . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| The studies on LAT , SLP 76 , ItK and Vav have shown their roles in the signal transduction of Ras and phospholipase Cx 1 to Ca2+ . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| Tec induced NFAT activation requires PLC gamma , but not the adapters LAT , SLP 76 , and BLNK , which are required for Btk and Itk to couple to PLC gamma . ^^^ |
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| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| In additions , we provide evidence that ZAP 70 is the primary in vivo kinase for LAT tyrosine 191 and that Itk plays a role in the phosphorylation of tyrosine 783 on phospholipase C gamma 1 . ^^^ |
|
| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and Q08881 |
Pubmed |
SVM Score :0.0 |
| NA |
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