| Recently , we isolated a novel protein , HAP 95 , that specifically binds to the nuclear transport domain of RHA and up regulates CTE mediated gene expression . ^^^ Here , using truncation and deletion mutations , we mapped the domains of HAP 95 that are important for RHA binding , transactivation of CTE , and nuclear cytoplasmic shuttling . ^^^ We report evidence for a novel nuclear export signal in HAP 95 and showed that the domains involved in RHA binding and nuclear localization are required for CTE activation . ^^^ Finally , we showed that HAP 95 synergizes significantly with RHA on CTE mediated reporter gene expression and promotes nuclear export of unspliced mRNA in transfected cells . ^^^ Taken together , these data support the proposal that HAP 95 specifically facilitates CTE mediated gene expression by directly binding to RHA . . ^^^ |